SDS-PAGE explained - Protein Separation Technique

Hey Friends,
SDS-PAGE (Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis) is used to separate proteins in a sample according to their molecular weight.
SDS binds to linearised proteins and masks the native charge. 1.4 g SDS bind per 1g protein, so that all proteins have a nearly similar mass-to-charge ratio.
After an SDS-PAGE, the gel is frequently used for Western Blot analysis to confirm the presence of a specific protein of interest.
Western Blot:
• Western Blot / Protein...
Here is a helpful explanation on SDS-PAGE:
ruo.mbl.co.jp/bio/e/support/m...
Thanks & Bye
Henrik

[@amrobay152]

wow, after each video, i ask myself "why professors can not explain it that simply", thanks a lot!

[@demaamro8750]

Are we related? 👀

[@nikpelekanou]

exactly that I'm Greek I study in Greek university and it s easier for me to learn all this in English than our notes that she gave us its not make sense she put so much useless details and they lost the mane subjects thanks for every one that really want to teach and not go to just do their job take the money and go

[@talkbiology01]

Because they have to pass the time

[@livh.7163]

Many professors are researchers first and foremost, but I agree it’s definitely an issue. They may be excellent mentors and teachers to their grad student researchers, but when asked to explain basic principles of biology to a class of undergraduates, they often falter. This is because 1) professors do not receive formal training as educators at the K-12 levels do (absurd right?!) and 2) there is no incentive to go out and seek that training in their own time. Professors are made to wear many hats, and the most important and pressing one is obtaining grant money to fund their research. The good professors people have at university are often those who either did seek some sort of formal training during their PhD/postdoc or who just have a knack for presenting complex concepts. What’s that mean saying? “Those who can’t do, teach.” Well at university it’s the inverse: “Those who CAN do, [often] suck at teaching.” 😆

[@rahman1314]

at this point , i think the prof don't understand the process, if you can't explain it this simply as in this video, then at least give us link to videos that explains it better to make our life easier

[@iguana1677]

Clear & concise. Well-done!

[@SaraMonicaLeonUlloa]

Excellent material! This is a great job explaining and illustrating the topic. Very clear information.

[@kimbokjoo6817]

I watch all the ads for Henrik. He’s been very helpful.

[@henrikslab]

Ahahahaha

[@galaxyeyes1457]

Thanks for making these nice videos. They’re very clear and the diagrams/animations are really helpful

[@wissammukaddam1393]

What a brilliantly concise explanation, thank you.

[@dermdoc3637]

bro this video is so good and so easy to understand thanks a ton dude

[@philbertnshimiyimana155]

I like this tutorial much. Very hepful in my current research project on p38 protein kinase

[@lotti7819]

thank you so much, you helped me a lot with my lab report!!

[@ahmetziyaaktas9179]

U explaining so clearly thank you 🙏

[@refad333]

Very accurate and clear explanation. Thank you

[@metarasouli]

Such a wow video should be appreciated. Thanks for sharing this video

[@jiinnyyk9483]

your video was so helpful!!! thank u very much❤

[@aqsanadir153]

You just made my day useful

[@henrikslab]

Well, that´s nice to hear!

[@omolemoratsoana4668]

Thank you so much 🙏🏽

[@fordmustang4945]

Thank you very much you just save my marks for viva

[@henrikslab]

Amazing!

[@razasyed575]

amazing video and explanation, thanks hoss

[@isadorah4969]

I want to become a teacher which explains clear and easy like u!!!

[@graemelaubach3106]

You make the most informative and easy to understand videos. Thank you :D

[@vivavampira249]

thank you very much!

[@3003wertpro]

Perfect Video!

[@bioscienceswithshahtareenswati]

👏👏Wellstone. Well explained

[@Matt-tv5ww]

this is the best SDS-Page explanation on the entire internet, thank you

[@dereksniper]

Great channel

[@iidmukhtarjama9245]

Your videos are absolutely amazing Please I didn’t see video about southern and northern blotting

[@storieswithmusab]

Thank you professor

[@user-le1yx1bu8s]

Super nice video!!! ❤

[@ellios5734]

thank you so much amazing

[@aliciacampelo5324]

thank you!

[@akhlasalarbi5690]

Wonderful ❤

[@sterlingteall3462]

Very well made video

[@ataraxie4086]

Danke!!!!

[@amooooeba]

God bless u

[@bighooligan100]

What site did you use to produce that sds image with the bands I’m doing my dissertation on cspg4 protein and have ran sds page for analysis and would like a neat version like yours on your animation as an image to my real life image

[@henrikslab]

Microsoft Powerpoint

[@salmaali-cf3od]

thank uuu for the videoo

[@bradding578]

But how do we take the separated protein out of the gel? or we cannot?

[@soulboken4670]

good shit

[@svitlanababych4051]

Can you turn on subtitles?

[@IlanHirschfield]

Very well-explained! Only comment/suggestion I have is that the negative terminal at the top is the anode, which is the negative terminal. The cathode is the positive terminal. The positive and negative terminals are labelled properly but the terms should be flipped in terms of their positions. Keep up the good wor!k!

[@washuttleworth]

This is the case for a galvanic cell it is reversed in electrophoresis, the positive pole is the anode. It is confusing but below is the explanation. In a galvanic cell, the anode is (-), and the focus is on pushing away electrons from the anode to supply power. In an electrolytic cell and gel electrophoresis, the anode is (+) and the focus is on attracting the negative ions in solution from inputted power.

[@biplabnayak315]

After watching this... i thought why my professor has to make every simple things present in a such complicated manner...why...

[@henrikslab]

In many cases, because they were never really taught how to teach...

[@laithibraheem7476]

but why cant we use a normal gel elektrofores for it. why must the protien be unfolded

[@henrikslab]

If the proteins are unfolded it is difficult to sort them accoriding to their size (in kD)... Why? Because some proteins may folded like a tightly packed ball whereas others have a longer structure... to make that equal unfolding is important. Apart from that, the unfolding is essential to mask the charges (this would not be possible when they are tightly packed).

[@josefinesfilmtube]

can you use this method to find protein protein interactions?

[@henrikslab]

This technique is part of another method that can be used for that: Immunoprecipitation... you direct an antibody against the protein of interest (want to find out the interaction partners of that protein)... you isolate this protein and everything that is binding to it. Then you can run SDS PAGE to see the size of the proteins you have pulled down with your target.

[@josefinesfilmtube]

@@henrikslab thank you so much for your explanation!

[@presleymumba5995]

I love you ❤❤

[@mad.i.mp3]

min 1:48 i cannot understand what he says: having the equal _______ help please

[@jasperpauli]

"the equal mass to charge ratio"

[@arathy592]

Wht does buffer soln contain.?

[@henrikslab]

Usually, for SDS one uses the TRIS-GLYCIN BUFFER: It contains Tris, Glycin and SDS (0.1%)

[@arathy592]

@@henrikslab and u didnt mention about bromophenol blue..glycerol..gel preparation...or is there any another video?can u help me?

[@TheLa0la]

Du könntest ein bisschen an deinem deutschen Akzent arbeiten :P Aber super Video!!! vielen Dank!!

[@user-lw7so7de6n]

you need to adjust the content

[@karthikmatta509]

Low Voice

[@emirkaplan9844]

I've seen some buffoons that couldnt explain in 30 mins. But all bro neded was 4 mins

[@BossAwesome11]

Isn’t anode negative and Cathode positive

[@ineskaineska8140]

لي جا من عند معماش يخبط جام 😂😂😂

[@Th3Cryton]

deutsches voiceover wäre auch nice :)

[@ffynab2733]

würde Sinn machen, es gibt nämlich kein vernünftiges Vid auf deutsch

[@michaelafehringer3679]

In der Wissenschaft sollte man aber schon Englisch können ^^

[@kohlrabenschwanz]

Sei doch froh dass du gleich bisschen scientific English lernst. Hilft auf jeden Fall wenn du mal vorhast papers zu lesen.