EMS Mutagenesis explained

[@1KittySugar1]

Thanks a lot for explaining something my professor couldn't!! honestly a lifesaver

[@dorrielycos1534]

Thank you so much for a simple and visually informative video!

Very well explained, congratulations. The only thing I miss is mentioning the DNA repair hypothesis :)

[@henrikslab]

Good point!

[@colbysmith4002]

Hello there! Because EMS is known to cause genetic defects and /or mutagenesis it could be applied to other plant species other than crop species, correct? Theoretically, would this work with something like monstera?

[@henrikslab]

Hello there, yes you are absolutely right! Crop plants were taken as an example. But EMS is also used with Drosophila melanogaster (fruit fly) or C. elegans (worm) to generate mutant lines. So, I personally don´t see why it shouldn´t work for other plants such as monstera. I would say: Yes

[@serjiviejoven9594]

1:20 You told about ETHYL methanesulfonate. but the you draw METHYL methanesulfonate

[@bennettgraff7883]

Maybe a dumb question, biology isn't generally my thing, but why do you have to wait for the second generation to screen for phenotype changes?

[@spulwasser]

Hi, point mutations, such as the ones caused by EMS, are almost always recessive. This means, that as long as the plant still has one allele without the mutation, the mutant phenotype won't show. The second generation is achieved by selfing a plant with the desired mutation (you can screen them for mutations before). By doing this some of the resulting seedlings will be homozygous, so have only the mutated alleles of the gene. As a consequence, there is no "backup" copy of a healthy allele anymore and the mutant phenotype will show, which is what you want to investigate/select for.

[@bennettgraff7883]

@@spulwasser thanks! That was super helpful

[@mahbubafatema7006]

@@spulwasser Hi, what do you mean by after selfing the seedlings could be homozygous, so does it mean the seedlings could be homozygous for having mutated alleles?