High Performance Liquid Chromatography (HPLC) - Operations by Dr. Sejal P. Gandhi

[@azhar17kkk]

I work on hplc and by far this is the best hplc demonstration I've found. Thanks ma'am

[@rajaryanbiotech]

First filtration followed by sonication since its role is to remove air traps. If you do first sonication again filtration then environmental air entraps the solution and you will get negative peaks due to air bubbles.

[@moabiekj8180]

I think that the vacuum created by the vacuum pump not only allows suction/filtering, but also catching any air in the solution. But you're right, first filtering and then sonication seems better. Have you noticed that the filter is smaller than the opening of the bottle and some of the solution may have flowed sideways, bypassing the filter?

[@shashiranjan2123]

Very much effective teaching method.....I tried to learn and understand the concepts.... thank you so much Ma'am

[@ahshanalikhan5708]

Sir now this is your turn to teach us 😅

[@varungowdahb9221]

Excellent teaching with instrumentation love from Karnataka ❤️

[@gaurihire2629]

Thank you so much ma'am By watching this video I am much clarified about the instrument 👍

[@LabKiBaat]

50 Subscriber completed. Thank you everyone.

[@parulsharma3143]

So much clear cut interpretation on this soft ware..thank u mam

[@matteof6340]

Does the vacuum pump has a trap? This content is absolute gold for the clarity by how it is explained

[@joydipbiswas-ki2hv]

Thanks for practical demonstration!

[@RAJGAMING5.0]

Mam excellent teaching ❤

[@sonalimahaparale4765]

Excellent information about working of HPLC

[@kalpeshnerkar4062]

Mam HPLC shimandzu LC CHT 2010 for the purging valve open krte hai ya nahi

[@chemistry9540]

it is very nice teaching . can you add auto- sampler injection ?

[@yaridosti-4867]

Thank you for the details explanation mam, I look forward to watch more videos like these. Best luck

[@rothmanfamilies7679]

can solvent be filtered if there is acetonitrile?

[@adhixxsanju]

What is the standard taken here?? And how did u arrive at the formula. Generally if we have a linear equation of standard samples of different conc we can find out the unknown concentration.

[@pratibhameshram6205]

Nice explanation mam, thank you for such important content

[@lakykhatun7119]

Mam how to choose mobile for diferent analysis like for vitamin B group and for vitamin E

[@hninmaylwin123]

Thank you so so much for excellent teaching

[@mohdimranansari5264]

Nice demo of paracetamol analysis........Is there any further video related to HPLC?

[@From_the_city_of_Queen_Jhansi]

Easy to understand in first view, Thank you Mam for the video

[@ashudada9482]

Nice explanation mam thanku so much mam

[@chitigori7349]

sir mujy hplc ka software chahye... sir i need the software of hplc.. ( Hitachi )

[@Safi_01]

How much blood required for HPLC

[@tandeldevang6083]

Nice way to present the HPLC

[@malathibalu7467]

very much thank you madam, very good explanation.

[@rachittyagi9436]

What is purge

[@Dr.B.Sunitha]

Removal of undesirable products

[@memories41941]

It's really helpful Mam Thank you

[@dr.reshama6427]

very nice explaination

[@sarojkumarraul3165]

Thank you so much mam 😊 for Amazing teaching 🙏

[@ydb6061]

Thank u so much mam plz make the videos on UV like as these video.

[@gutemajebe4365]

IS THERE ANY EQUIPMENT THAT CAN REPLACE IT?

[@anupamshukla803]

Maim apka sop or guidelines copy mil skti h

[@rajasaravanan1967]

Calibration curve start with 1 MCG 2 MCG ....... 5 MCG. But actual concentration 5 MCG , 10 MCG,...... 50 mcg

[@suryaindian8457]

Very nice मैम, upload more videos. 👌👌👌👌I am working in pharmacetucal industry (laboratory)

[@Life-journey-vivek]

Hello Reply me I have to asked some questions

[@swathinanu665]

Excellent mam 👏👏👏

[@boringperson9779]

Hamare college me to ye sab sikhaya hi nhi gaya

[@pharmapreparations]

For more details for Pharma industry interview preparation channel

[@sonajigaikwad6863]

Excellent session

[@amolgujar211]

Nothing like actual in company

[@Prince-hv8di]

Instruments use krna difficult hota hai kya bro???

[@shrinivasshinde2001]

Everything is same

[@vaishnavidhimmar1027]

Great video.

[@akashnawkhare1785]

U should sonicate the m.p after filtration, not before that

[@carlosjust83]

Can you helpme to operatehaplc?

[@sanjaysanjaygomtiwal4453]

Yess bro !

[@aashupatel1003]

First sonicate the buffer solution then filtration then sonicate then make mobile phase

[@MEMEMAKER-l6q]

​@@aashupatel1003 where can i learn full hplc freee i dont have that money to learn for method develeopemnt and validation by rp hplc

[@sheila4139]

Watching from kenya

[@mohammedraheesmuthanikat3258]

Is Any vacancies available?

[@aynomwolda8250]

Nice explanation , thank you .

[@mariahassanmariahassan6536]

Excellent Madam

[@ketanvyas1566]

Nice info mam✌

[@ganeshyeole5902]

Nice explanation mam👍

[@knoweasy]

Informative session 👍

[@MUSMAN-pc6wf]

Good job mam.

[@ajithpharmacist6204]

Excellent teaching mam I'm so happy tqq so much

[@kamleshthakur3871]

Nicely explain

[@puspitachal9314]

Calculation part is not clear..

[@amitamitkumar1261]

Real

[@gulshansinghrajput9403]

Supper ❤️

[@aasmohammadsaifi7068]

Thanks mam

[@ko-fanta]

Thanks 😊

[@VivekKumar-lj1ft]

Can u make this vedio in hindi language

[@pharmasolutionpakistan7668]

Good effort

[@educationpoint778]

appreciated from china

[@pharmapreparations]

Nice

[@sowmiyashanker150]

Thank you mam

[@safiahameed1536]

Explain it deeply kindly an Jazakallah

[@LailaAlly]

Thanks more

[@qamaruddinast5696]

👍

[@nirajpatil3757]

👍👍

[@naturevibesbypatil]

Are hindi mai explain karana chahiye tha indian hokar english mai explain kiya bakiyoko bhi samaj aana chahiye......

[@ChaunceyPrevatt-s5s]

Jones Sandra Harris Scott Hall Anthony

[@MaheshYadav-z5g]

Hi

[@dr.nileshjadhav9569]

Go slow ur are talking as if u have rattofy it

[@VirendraKumar-ni5ss]

Your lab is very old model use in instrument

[@smruti011]

Thank you