High Performance Liquid Chromatography (HPLC) - Operations by Dr. Sejal P. Gandhi

This video is a virtual tour to Shimadzu HPLC system available at Central Instrumentation Facility of Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune. Viewers will be able to understand Standard Operating Procedure of HPLC system. It involves all practical aspects in operation of HPLC by demonstration practical of Assay of Paracetamol by RP-HPLC method.

[@azhar17kkk]

I work on hplc and by far this is the best hplc demonstration I've found. Thanks ma'am

[@varungowdahb9221]

Excellent teaching with instrumentation love from Karnataka ❤️

[@parulsharma3143]

So much clear cut interpretation on this soft ware..thank u mam

[@sonalimahaparale4765]

Excellent information about working of HPLC

[@yaridosti-4867]

Thank you for the details explanation mam, I look forward to watch more videos like these. Best luck

[@gaurihire2629]

Thank you so much ma'am By watching this video I am much clarified about the instrument 👍

[@hninmaylwin123]

Thank you so so much for excellent teaching

[@rajaryanbiotech]

First filtration followed by sonication since its role is to remove air traps. If you do first sonication again filtration then environmental air entraps the solution and you will get negative peaks due to air bubbles.

[@moabiekj8180]

I think that the vacuum created by the vacuum pump not only allows suction/filtering, but also catching any air in the solution. But you're right, first filtering and then sonication seems better. Have you noticed that the filter is smaller than the opening of the bottle and some of the solution may have flowed sideways, bypassing the filter?

[@matteof6340]

Does the vacuum pump has a trap? This content is absolute gold for the clarity by how it is explained

[@shashiranjan2123]

Very much effective teaching method.....I tried to learn and understand the concepts.... thank you so much Ma'am

[@ahshanalikhan5708]

Sir now this is your turn to teach us 😅

[@pratibhameshram6205]

Nice explanation mam, thank you for such important content

[@mohdimranansari5264]

Nice demo of paracetamol analysis........Is there any further video related to HPLC?

[@tandeldevang6083]

Nice way to present the HPLC

[@aynomwolda8250]

Nice explanation , thank you .

[@malathibalu7467]

very much thank you madam, very good explanation.

[@chemistry9540]

it is very nice teaching . can you add auto- sampler injection ?

[@vaishnavidhimmar1027]

Great video.

[@adhixxsanju]

What is the standard taken here?? And how did u arrive at the formula. Generally if we have a linear equation of standard samples of different conc we can find out the unknown concentration.

[@sonajigaikwad6863]

Excellent session

[@memories41941]

It's really helpful Mam Thank you

[@swathinanu665]

Excellent mam 👏👏👏

[@ashudada9482]

Nice explanation mam thanku so much mam

[@ydb6061]

Thank u so much mam plz make the videos on UV like as these video.

[@suryaindian8457]

Very nice मैम, upload more videos. 👌👌👌👌I am working in pharmacetucal industry (laboratory)

[@knoweasy]

Informative session 👍

[@RAJGAMING5.0]

Mam excellent teaching ❤

[@kalpeshnerkar4062]

Mam HPLC shimandzu LC CHT 2010 for the purging valve open krte hai ya nahi

[@From_the_city_of_Queen_Jhansi]

Easy to understand in first view, Thank you Mam for the video

[@rothmanfamilies7679]

can solvent be filtered if there is acetonitrile?

[@sarojkumarraul3165]

Thank you so much mam 😊 for Amazing teaching 🙏

[@ganeshyeole5902]

Nice explanation mam👍

[@LailaAlly]

Thanks more

[@ko-fanta9829]

Thanks 😊

[@ketanvyas1566]

Nice info mam✌

[@MUSMAN-pc6wf]

Good job mam.

[@dr.reshama6427]

very nice explaination

[@sowmiyashanker150]

Thank you mam

[@lakykhatun7119]

Mam how to choose mobile for diferent analysis like for vitamin B group and for vitamin E

[@mariahassanmariahassan6536]

Excellent Madam

[@kamleshthakur3871]

Nicely explain

[@pharmasolutionpakistan7668]

Good effort

[@nirajpatil3757]

👍👍

[@safiahameed1536]

Explain it deeply kindly an Jazakallah

[@rajasaravanan1967]

Calibration curve start with 1 MCG 2 MCG ....... 5 MCG. But actual concentration 5 MCG , 10 MCG,...... 50 mcg

[@gulshansinghrajput9403]

Supper ❤️

[@educationpoint778]

appreciated from china

[@gutemajebe4365]

IS THERE ANY EQUIPMENT THAT CAN REPLACE IT?

[@qamaruddinast5696]

👍

[@amolgujar211]

Nothing like actual in company

[@Prince-hv8di]

Instruments use krna difficult hota hai kya bro???

[@Safi_01]

How much blood required for HPLC

[@chitigori7349]

sir mujy hplc ka software chahye... sir i need the software of hplc.. ( Hitachi )

[@ajithpharmacist6204]

Excellent teaching mam I'm so happy tqq so much

[@mohammedraheesmuthanikat3258]

Is Any vacancies available?

[@boringperson9779]

Hamare college me to ye sab sikhaya hi nhi gaya

[@akashnawkhare1785]

U should sonicate the m.p after filtration, not before that

[@carlosjust83]

Can you helpme to operatehaplc?

[@sanjaysanjaygomtiwal4453]

Yess bro !

[@anupamshukla803]

Maim apka sop or guidelines copy mil skti h

[@VivekKumar-lj1ft]

Can u make this vedio in hindi language

[@rachittyagi9436]

What is purge

[@bsunitha118]

Removal of undesirable products

[@puspitachal9314]

Calculation part is not clear..

[@amitamitkumar1261]

Real

[@naturevibesbypatil]

Are hindi mai explain karana chahiye tha indian hokar english mai explain kiya bakiyoko bhi samaj aana chahiye......

[@VirendraKumar-ni5ss]

Your lab is very old model use in instrument

[@dr.nileshjadhav9569]

Go slow ur are talking as if u have rattofy it