How a gene gun works

Kyle Taylor from the Glowing Plant project explains how a gene gun, which is used to transform cells with synthetic DNA, works.

[@JulianoVitiello]

Here is a good tip: wrap electric tape on the top part where you screw in the rupture discs. That way it wont sound like nails on a chalkboard each time.

[@lakshayaindrapugal542]

Thank you soo much. Actually I was thinking how does this thing work?. It was referred as " bombarded " in my book 😂.

[@anushankar8364]

Neet aspirant things😅

[@devikadevuz4612]

NCERT😂🤍

[@mewantbrains]

I believe in the video you said you will demonstrate it firing I don't see that anywhere in the video

[@DJMouthwash]

***** 0:28 seconds.

[@mewantbrains]

That's where he says he will demonstrate the firing process but it did not actually fire

[@TAXABiotechnologies]

It does fire, it's just not that dramatic!

[@ashdroid]

The machine is turned off and not running at that specific time 0:28, it even has the door open. EDIT: I just saw your other video.

[@abefromansausagekingofchic6480]

Riddlemethis yea I was kind of blue balled too. Found the other video though

[@birk_lab4805]

You stopped before the bombardment! WHHHHYYYYY??? :,,-(

[@KatherinePierce_81]

You serial killer

[@realryder2626]

Obviously he got a txt and had to reply

[@adventuresoflife486]

If there is no vector present we use Gene gun/bolistic for plant and microinjection for amimal... 🌷❤️❤️

[@DipanshuGhosh]

You should have demonstrated the whole process. You didn't fire the nano particle coated with preferred DNA.

[@lolpotato]

Generally gold or tungsten with attached desired gene is used to fire into the vector. This is what i have been taught but never saw it in practical

[@Noveshd]

​@@lolpotato yep its only done when u choose biotechnology as career.....not in medical also

[@callahancovington4278]

It would be nice if there was a laser to help you align the tissue directly into the gun's trajectory.

[@theashmedai007]

Can u combine mamels with plants

[@blazearmoru]

aww. you didn't show us the process with the onion D: i hope it's in the next video ^~^

[@KaitokiNohara]

Now I know how it works

[@kellycarpenter9350]

Hey I would like to learn how to inject flavonoids into plant tissue to create flaver into plants .like strawberries..I want to cross flaver with pink pepper etc. Kel

[@axanachernyavskaya3556]

thanks a lot! very useful!

[@Ekergaard]

I have heard a Ph.D. student say: "This is our nanodrop there're many like it but this one is our". So it's temting to ask if anyone has said: "this is our gene gun ..."

[@PrasetyoMuhammadDwiBiology]

real men use heatshock :D

[@jazzmessenger8]

What genes were on the tungsten?

[@jayesper4390]

Bill Ricci Dunno, but gold's the way to go. Tungsten should be out of the question.

[@FoxCurtailed]

It looks like a tiny, old school gas pump.

[@deborahjudson350]

It does not ever fire

[@sapnamenghwar8219]

Oh I was waiting for them to bombard the tissue, just to see the machine in action 🤔

[@biologyinhindiandenglish8787]

Ho sakta you can follow any responses to you and

[@smilesinger3652]

Guys who are from vyshavi college 😀

[@NavinJohnson_thethird]

Dr. John Sanford waa the primary inventor of the gene gun. A former Atheist who became a Christian.

[@David-ck3gv]

The prototype was also just a .22 caliber that shot blanks into plant tissue lol

[@timdwyer3516]

Anyone here from 7/8 b

[@KatherinePierce_81]

Do you need a gun license for this?

[@AntonyEvansUK]

No

[@sarahross975]

you arent shooting anything, it wont hurt you very badly. Dont shoot yourself with one but still

[@MinxInSinx]

@@sarahross975 dose it change your genetics or the lineages of your genes? Yes!!! Known as gene frequency/allele frequency The human body is natural IMPERMEABLE!! The way all humans are made! You think it's wise to make yourself vulnerable to foreign genetics 🤦🏻‍♂️😂 You can't be that arrogant(can you) Natural immunity has been observed to be successful 99.93% of the time When you change how your body is supposed to respond you increase the death toll! Due to mutations > these mutation result in illnesses and diseases & metabolic diseases *CANCER Death in lab rats = death in humans 💔 Biolistics are used on plants & people! ( vaccines ) to change the genetics > you think eating those food isn't going to change your genetics or the lineages of your offspring 🤦🏻‍♂️ If you knew your work was killing people would you continue your career Choices?

[@sarahross975]

@@MinxInSinx its been proven that eating gmos isnt bad stop living in the past

[@ducktheduck9792]

kuku

[@zombiebillofthedead]

Filming on a phone I see, wouldn't have known if the picture didn't shake and make that vibrating phone sound. Not that it matters.

[@user-vw9jl9du9y]

الي ما يعرف انكلش شلون يتابعة🥺

[@balbaks]

بتقدري تدوسي على علامة CC وبعدين روحي اعدادات الفيديو > الترجمات > ترجمة تلقائية، هي فيها شوية تخبيص بس راح تساعد بالفهم

[@user-vw9jl9du9y]

@@balbaks تطلعلي كتابه انكلش ما يصير عربي ؟

[@balbaks]

@@user-vw9jl9du9y لا موجود عربي بس ترجمة تلقائية بعد ما تدوسي على CC روحي لاعدادات الفيديو (إشارة المسنن) واختاري ترجمة تلقائية أنا فاتح من الحاسوب ممكن من الهاتف يكون مختلف

[@user-vw9jl9du9y]

@@balbaks يمكن الموبايل يختلف راح اجرب الحاسوب شكرا جزيلا الك

[@anusandhanwithdevantiwari181]

Can u tell me hindi

[@christurnblom4825]

politics of gmo vs non gmo aside, I find it humorous that I've actually heard a scientist on the radio say that this is no different than what farmers have done for thousands of years when breading plants for a desired outcome. This, in response to being asked what he has to say to people who are concerned about the safety of gmo's. They weren't taking calls but if they had been my question would have been "What force of nature causes a similar effect to a gene gun and achieves the same result?" Whether or not there is net harm to the environment aside, I just found it insulting that he would play on people's ignorance like that.

[@christurnblom4825]

So one of those you mentioned, blasts the same "clip" of DNA into other DNA at over 600 mph, blasting apart the DNA while getting random pieces to stick in the desired place? I guess I do have a lot to learn.

[@christurnblom4825]

What are you talking about? Sarcasm is great! Example: Damn you for knowing stuff & making me think about what I've said!! Sarcasm helps me feel like I'm doing well while your schooling me. ...and it's occasionally funny. All I got left is the thing I put aside at first ie my concern that being selective in that process has a different outcome than in nature or even when we selectively breed in the old method because we don't have all the other iterations that would result, out their doing their thing. I doubt we have any tech. that can figure out if those two ways are any more or less harmful to the environment than the gene gun method but my hunch is that it probably puts things out of balance. At the same time, I'm pretty sure that nature will just shake us off or swat us with her tail and go back into balance if we become too much of a problem.

[@FringeWizard2]

I don't think there is a problem with genetically modifying organisms but the specific traits they choose to develop in plants are often really stupid traits they should not have aimed for, whether it be through selective breeding or GMO. Stuff like tomatos that produce more crop with the same amount of nutrients; the result is tomatoes that have significantly less nutritional value. GMOs plants the produce their own pesticide are also a stupid "innovation" as pesticides are often (not always) not very good and harm our health. When they try to develop plants with commercial interests in mind it often times has a lot of drawbacks. It's not also just GMO that is bad but many varieties of plant which are grown for commercial production are in my opinion inferior to heirloom varieties developed by gardeners who just wanted a tasty or beautiful tomato.

[@selenaburke2956]

There are foods you eat with transgenes that got there naturally. like potatoes

[@MinxInSinx]

@@FringeWizard2 do you think it is ok to genetically modify your genetics & the lineages of your offspring? The only way for your dna to change is if you inject it into the user. We are what we eat

[@user-nr6kc2ie4k]

PDS1000$$

[@miguelpatrao8999]

ahhhhhhhhhhh cromo