How to Build a Laboratory (for CHEAP)

[@Exzeph]

Hooray more Glorb videos :-)

[@TheTubejunky]

Used an old PC fan with neodymium magnets glued to it and made a DIY magnetic stirrer. Used a 10k potentiometer for speed control.

[@lukerXIII]

Bro 😎 that's awesome 💯💯💯💯💯

[@ronrampfel2197]

Genius my friend.

[@lukerXIII]

@ronrampfel2197 right lol

[@SmirkInvestigator]

Can’t skimp on precision control

[@nittani.]

Explain how to do it im genuinely interested for an animation im making

[@wraith313]

Honestly im pretty impressed because your home lab is more equipped than half the ones i worked in when i was in grad school.

[@SteveEh]

I was about to despair, but then, you said not to, so thank you for that!

[@Vinc94Machinima]

As a mushroom grower. We basically have the same setup. If you need cheap lamilar flow hood look for mushrooms growers. We usually are forced to build it ourself so we are pros at it xD

[@walkerfeet8729]

Since people are sharing... I built a laminar flow hood for the cost of the filter, $100. Repurposed the blower motor from an old downdraft kitchen island cooktop, and some discarded white vinyl wrapped kitchen cabinet doors. I'm not a braggart but i'm proud of that. I lust for an autoclave. So tired of pressure cookers. Have some idea to make a pressure sensor to control a hotplate with a timer, so i can set it and forget it. Yeah, it's not a good idea. Also I set my first cardboard box SAB on fire with an alcohol lamp. Fortunately, no one saw me throw the little inferno onto the suburban front lawn.

[@alreadyghosts7727]

You got specs or designs for that cheap version of a lfh?

[@L0wSkiller]

​@@alreadyghosts7727replying to poke the original poster, even if its incredibly hard to read handwriting / hand drawn, you've piqued my interest :)

[@clutchboi4038]

It's a good idea just put a pressure release valve for added safety. But tbh I got mine dialed in most of the time where I just set the knob and forget it. I usually run it hot af until it builds up pressure and then immediately turn it down to a set level that I eventually discovered works with my burner and cooker and just walk away and I can tell if it's burning too hot by how much the weight wobbles. You don't want it going crazy and a small wobble is all you need. The main thing is you need to run it hot enough to get the pressure built up because it won't reach that pressure at the pressure maintenance heat level. Once you do it enough times it becomes second nature.

[@christophermiller2075]

I totally relate! I caught my homemade Still Air Box on fire too…although I was using a mini torch to sterilize tools and I would hose down the inside of the box in alcohol between uses. Things were more prohibitively expensive back then so I did what I could but not using an open flame is honestly for the best, if at all possible.

[@lilyinthewater161]

Do you think a uv lamp within a sab will work.

[@cosmicaug]

9:04 Regarding using the Instant Pot as a sterilizer, at least one model will work if you make sure to increase times. See _Assessment_ _and_ _Verification_ _of_ _Commercially_ _Available_ _Pressure_ _Cookers_ _for_ _Laboratory_ _Sterilization_ (or DOI 10.1371/journal.pone.0208769 ).

[@mikeselectricstuff]

Aliexpress is a good source of very cheap equipment and consumables- quality can vary but typically cheap enough that the occasional bit of rubbish isn't a big deal

[@angeltorres9372]

Hello Glorb, I stumbled on to your channel last week and I am intrigued. My some is working on his micro biology PHD and when he talks about I am lost. What you're presenting is something I can do and can have a descent conversation on the subject especially the tissue cultures and cloning. Thanks for sharing looking forward to watch all your current and future content. 😊

[@Milkex]

I make induction heaters for like $30 which are great for scalpels, inoculation loops, syringe needles, etc. never had an issue with contamination

[@Brick001]

Video?

[@Milkex]

@Brick001 nah, it's extremely common and you could find 50 with no effort. if you're cool you'll look for a dynavap induction heater diy plan

[@Brick001]

@@Milkex thanks for pointing me in the right direction!

[@Sporophore_Hunter]

Induction sterilizers for the win 🙌

[@gargoylekingGWO]

Alot of knowledge dropped on this one

[@JohnWeland]

A whole segment on KZbin I didn't even know was a thing. I am intrigued now

[@collaborativeclockworks]

I used your recommended shelves and liners.. good call, thanks.

[@ghostmantagshome-er6pb]

When I was a teen I took some of my moms houseplants and took cuttings. I put them in little medicine cups with different nutrients in them and the one with the most root growth was the one with artificial sweetener. Don't remember what kind. about 1980. This is actually what i did when I had the house alone for an entire week. Party.

[@kathykickingwoman9120]

That is really interesting! I wasn't that curious then, but as my old a$$ gets older I am wondering why ...why was I such a heathen lol. Thanks for sharing that!

[@h1234e1234]

Nice

[@therandomguy4988]

Hard drives go spin very fast. Fans also spin fairly fast. If you want spinny lab devices of either variety, I recommend these. Be safe with hard drive spinny! If it breaks the shrapnel may go fast enough to punch a hole in you.

[@TheTubejunky]

For the sterilization of scalpels use an induction heater. Much cheaper and do exactly the same thing.

[@charliemagoo7943]

How do you know when to stop with the induction heater??? When glowing red??

[@TheTubejunky]

@@charliemagoo7943 Yes!

[@anonimoqualquer5503]

using a thermometer or just setting the configurations right turns out is very easy to configure a induction heater for Just the right temperature .... and they are REALLYA cheap, like daam china the forced labour is off the chart

[@M-PASTA]

Don’t know anything about plants or how this was recommended to me but the sense of humor is A1 so have a sub 😂

[@xToTaLBoReDoMx]

everything glorb puts out has been A1 so far (especially the music videos) so I'm not surprised

[@ChuanfaBuddha]

Awesome, I appreciate your content. I learn so much. Can’t wait for the affordable home videos. Thank you

[@gefginn3699]

Greetings Laur, I just found your channel. So happy to see you here. Very interesting. I have some binging to do getting comfortable with terminology, etc. Happy Holidays! 💛😇

[@smallhands2160]

Best and most informative video Ive seen in a while. Subscribed immediately after I heard about the surplus websites

[@glenndhooge6787]

ther is indeed a big upfront const. But we are talking here about an actual lab. not a small hoby setup. I love the fact that we get an insight in your actual lab setup. It is not something you're able to see every day as an outsider. Also absolutly love the comparering between how most people think a lab looks like vs how an actual lab looks like 😜

[@cameronlee-ming5249]

"Trash goblins" 🤣 I feel attacked

[@WEB4plus]

Awesome guide, thank you for sharing!

[@Neuroguy100]

Thank you so much for making this video! I have been buying equipments brand new.

[@harryhashin]

QVC style was very helpful thanks Glorb

[@mitomon]

what is it

[@bhuvanachandrabr4890]

Thank u so much for the hope ! I truly appreciate it, I had to see like 20 of your videos to have hope for my research and a farm to execute the growth process ! I sincerely appreciate it

[@yearofthegarden]

This is great, I am about to build my lab again, but it's specific task is for mycology bulk inoculating. I am going to try and make it as small as I can so it can be on a porch, probably 6x6ft with a pass through atmospheric steam sterilizer in the wall so I can load large batches of substrate sterilize and open it up clean inside. It is made out of a Aluming baking proofing rack with 24" aluminum flashing and foodgrade high temp silicone. The bottom has a steam pan with a 2000watt water heating element and stainless steel float valve. I used to do spore to grow, but the amount of space for dishes, slants and fridges is to much upkeep and space. I dont miss dealing with flaming rubbing alcohol which is why I've outsourced that aspect of the process, just make sure you have a small can of fire extinguisher, they make aerosol sized. This video is defiantly getting me excited to start lab work again. It's a good skill to know for niche product productions, but someone like me who has moved twice this year, it's hard to have space for a lab, particularly because houses in general are almost worse for spore load than outdoor air. I used to do my mycology outside at 2am when the air is still back when I started, because that is the calmest time with the lowest spore load. That can be dealt with by having a well made room and positive pressure HEPA filters though.

[@nosuchuserid]

Thanks for posting this video. I have a Corning stirrer/hotplate that costs about the same as the Fisher isotemp you have. I think the reason for the high cost of these models is that, at that price point, they come with a temperature probe so you can have it monitor and adjust the heat output to maintain a specific temperature. It's not a feature I've used a lot, but it has come in handy at times. The other thing I can comment on is the Ohaus Scout vs the cheaper scale that you mentioned. I have both and I much prefer the Scout despite the higher cost. The really nice feature of the Scout is I can leave it running all day and it doesn't drift like the cheaper models tend to do. Mine came with a power supply, so I don't have to worry about batteries either.

[@chrispycryptic]

Thank you SO MUCH for this video! Those websites are amazing.

[@jimparsons6803]

Well done. My efforts don't rise to the level of your efforts; I use a selection of test tubes and glasses of warm water with corks for the test tubes followed by decanting. I fool with sents and flavorings. I have been thinking of trying for distillation, though. Thanks for your advice and insights.

[@SIM31r]

My hobby is electronics. I plan to have a laboratory, but something simpler. I have a magnetic stirrer, some chemicals. Three 3D printers, a laser engraver, a CNC milling machine for milling printed circuit boards with a 0.1 mm cutter. But in the video the laboratory is better and the projects are more interesting 😀

[@timbambantiki]

oh wow this plant thing is cool. subscribing

[@stevef3521]

Ultum Nature Systems is another aquarium plant tissue culture company. They’re based in the US. You mentioned an overseas company in a past video. Propagating and researching “dry start” planted aquariums (inexpensive way to grow carpet plants) ended up with you in the algorithm. PLANT ON! 🤓

[@plantsinjars]

I’m working with some aquatic tc plants for the first time ever tomorrow 😄

[@stevef3521]

That’s awesome! Couldn’t have timed that comment any better if my Micranthemum Tweediei’s life depended on it. 😂 Any chance we’ll see a video on it?

[@Mark-rg1qh]

This was a very informative video, thank you for your efforts.

[@romualdaskuzborskis]

Ok, im sold - subscribed.

[@olleassistans7467]

I'm not into biology, but it has some resemblance of electronics labs, i turned into electronics land :) You have the same desire to do your thing as people in my field have, good to have you on the same planet :) One can see the happiness.

[@assertchris]

I don't understand any of this, but you're an engaging speaker and I ang I sub on this basis.

[@JeronimoStilton14]

This seems quite advanced for just growing a few plants. I am going to start watching your other videos to find out what makes them special lol.

[@PlantCellTechnology]

Loved this video! Flashbacks to *you ARE the hot plate*

[@FMFvideos]

Men of science, we are united again.

[@JP-vx2sr]

I have a chinesium stir plate from ten years ago that i bought for jump starting yeast cultures for making beer. Still works. No hot plate though.

[@parkerellis5716]

I just saw this video on my homepage. ❤ Following.

[@Primitivemycology]

Love your channel!!! I have multiple 23 quart PCs and two 53 liter industrial PCs nowadays, but I started with an insta pot several years ago. They usually achieve 10psi and they work just fine. The time must be extended to get the same results IE a ratio of 1:1.7 is the technical adjustment for the loss in pressure

[@ashishacharya0001]

Good format with humor.

[@J_Quinn_Creates]

Shout out to government surplus and trash goblins 😊

[@aaronwilliams7062]

The 60hz hum of that autoclave was satisfying

[@PlantyMcPlanterson]

I continue to watch all your videos they are so helpful. Thank you so much!

[@PatrikL456]

I watch this with great interest even though I have no concrete plans whatsoever to cultivate plants this way (I mostly do hydro). I don't know if it is you presenting it so well that it becomes interesting or if it is just the topic itself. Well done either way!

[@Organize-pv7ys]

I’m so grateful for your hard work

[@infiniteworfare-el3hi]

i have more respect for a video gamer cause at least they arent torturing plants like this. we shouldnt alter plants when we dont know plant physiology. they are living and sentient organisms.

[@alldecentnamestaken]

Not going to lie, the also fed this to me randomly. This isn't my hobby or interest but the intro was great so I subbed.

[@TheTubejunky]

I bet you would fit in with us Mycology ppls just fine! Similar clean works. Awesomeness!

[@whatabouttheearth]

You mycology people are as obsessed as the birders 😂

[@whatabouttheearth]

You mycology people are as obsessed as the birders 😂

[@brooksmiller5597]

Your merch design is *_really cool_*

[@JP-vx2sr]

How did KZbin know this was up my alley. Many levels of catnip in this video

[@alreadyghosts7727]

This is great! I am interested in doing very basic experiments with soil microbes.

[@johanjotun1647]

I'm trying to get the fixins to start my mom on tissue culture for Xmas, she was growing a leak suspended by toothpicks at the surface of a jar of water, and my everpresant desire to expand and complicate things lead me to you, the biocoupler kit is on its way, but my budget says the flow hood, stirplates, pipepettel she will have to do with out...Hopfully theres some luck to be had with a primitave go at it.

[@Asterrayx]

cool stuff glorb thanks for this information that I will maybe someday use

[@EarthlingNews]

Thank you so much

[@careyearthman3421]

I flipping love lab equipment. Don't feel bad about falling for the brand new Isotemp. We use them in one of the Micro labs (for college students), and I would have so much trouble denying myself the pretty pretty (except I'm broke). We had a terrible time with our autoclaves not getting up to temp or not staying at temp long enough (while we were waiting on a whole new set of them to be bought and installed), and multiple labs bought Instant Pots as a backup. We still use ours when we have very small things to sterilize and we want to save some energy. (This is a BSL 1-2 lab where we need to sterilize media for bacterial growth and a genetics lab that I think uses agar plates with their C. elegans.)

[@phillipsmith21]

I'm looking to build a small lab for our family farm, that's what brought me here. Now I'm looking at an Autoclave thinking I can pressure can a lot of food in that thing. 😆

[@keeganmaclean]

MORE VIDEOS PLEASE ❤

[@Ciclify]

What. This is so cool. I don't know why this was recommended to me but would I build a lab? Yes.

[@rmt3589]

I made a joke Wednesday about not having a lab. Have a degree in Chemical Technologies I don't use. Plants aren't my thing, as I can't keep Mint alive, but the plant stuff looks cool! I prefer scalpels. I'm cursed(not literally) and can't use scissors precisely. Love exactoknives and scalpels.

[@michaelriddick7116]

QVC was so amusing to watch at 3:15am 😁🤣😂🤣😂🤣

[@Saiper1990]

thanks for this

[@sviatoslaviigorevich7360]

Hey, I know you said that you can tissue culture any plant, but what about mosses and lichens? Are those even considered plants? I love moss, but it grows so slow. Could tissue culture be a faster way of cultivating mosses/lichens?

[@Speed001]

Cool, very helpful

[@kevin_horticulture]

orbital shaker's got some moves

[@Speed001]

5:27 orbital shaker getting groovy

[@anotherguy9402]

If the instapot has an exposed wattle you can add weight to help achieve the 15psi but depending on the manufacturer the gasket might disagree

[@chinupduck4849]

Omg. Public surplus? How did i not know about this. Hold me back.

[@TDogsYard]

You should check out safety scalpels if you need to make some very tiny specimens or what not. Fisher scientific has a nice one, but a bit pricey! 😝

[@dowhilegeek]

scalpel lore/trauma continuing to make valuable content ⚰

[@abdulkhalid6596]

Does temperature play a significant role in tissue culture? How can we successfully cultivate plants from different regions of the world, each adapted to specific temperature ranges, and enable them to thrive in controlled environments like containers in varying climatic conditions?

[@hashmaster27]

Is it possible to propogate black mondo grass via tissue culture? Also, thank you for your very informative videos!

[@LakelandRussell]

Where were you forty years ago when I needed to know this. LOL

[@timbone4925]

Hello Laur, great videos, well presented! have you heard of the Wollemi Pine? (the dinosaur tree) do you think it would be possible to use your techniques to propagate this plant?

[@curtiscampbell2558]

Awesome!!!

[@worldboy9684]

Omg, perfect guide

[@brentboudreau4360]

I love your videos, very informative and your sense of humour is the best 😊…..have you ever used Keiki Paste, and if so, what are your thoughts on it? Thanks!

[@fairbanj]

lol I have no idea what this is but I enjoyed the vid... very cool you are doing this. I've subbed and will lurk some 🙂

[@mrrith6840]

here in asia, i use to try and make a lab but after trying to set it up for a while. that when i realize I don't have enough buget for it even for a super small one as for 1 small machine like sterling machine, and gas autoclave cost like 1 pay check per machine 😢

[@hurakann]

Your lab is incredible, someday I'll have one too, for now no house, no lab, just avocado because in Brazil it's free kkk

[@scotthenry3401]

What about a UV steriliser or a silver ion? Sterile oven, i think they are used in operating rooms

[@robert-wr9xt]

Fun stuff. Thanks

[@abcramirexz25]

Educational ,humor and funny

[@Mochi-wf3ys]

would it be possible to make a video on testing different sterilizing methods for explants? ie. bleach vs UV vs bleach fumes vs ozone

[@trontrontrontron4]

love this how to make your own covid-19 series

[@KarlKarsnark]

Is there a reason you use the older "blurple"(purple) growing lights in some areas, rather than using white, full-spectrum throughout? Depending on how precise the pH readings need to be, there are also rolls of pH paper tape(litmus paper) that change color to indicate the pH of the solution. It can be accurate to within .5, if that is sufficient. Cheers from up the road in N. FL. Go Noles! ;)

[@isazisempi3896]

when you said horrors humanity wasnt meant to see I instantly subscribed. But you knew that would happen didn't you.

[@johnnylego807]

Does the plant cloning work across many species? I have some extremely rare specimens and the first part of your video got me excited if this may be a possibility. Any info would be appreciated. Got my sub.

[@plantsinjars]

Yes it does!

[@johnnylego807]

@ Thankyou! I know this is rather specific but do you think it would work with monocotyledons, meaning they have a single cotyledon, or embryonic seed leaf. Type plants, specifically herbaceous flowering plant of the arum family? Thankyou for the replies.

[@robertfrazee232]

I love the top!!!! Venus fly TRAP!

[@ianfarrell1182]

If you don’t want to use a laminar hood just work under your stovetop fan:) same goes for hot plate

[@HomeDistiller]

there is a 4th type of sterilizer that is good for anything slightly larger than a loop, and thats an induction sterilizer, they are very fast and zero heat up time

[@charliemagoo7943]

How hot do you get the items in induction heater,???? Just stop when red or what,,????

[@kevin_horticulture]

I'm so ready to make this.

[@swim2]

I like plants and jars and plants in jars

[@samshanker5753]

8:35 " heat to sterilize material " So I will use oxyacetylene torch😅 or can I..?

[@JKTCGMV13]

That transformer joke was definitely hilarious for sure

[@unconditionallove3820]

Makes more sense bumble bee would turn into something that helps plants along.

[@thrawn8840]

Very interesting

[@xspager]

I learn so much by clicking on videos