How to Grow Cerebral Organoids from Human Pluripotent Stem Cells

[@Charlotte-wv1dl]

I have no idea how I got here and I don’t understand any of this but I was entertained as hell

[@jonathanrivera1607]

My man a year later and another fell victim being amused by science I don’t understand😂😂😂

[@Xisk77]

Same! Are they making stem cells ?

[@arcturus4067]

@@Xisk77 no. They use human stem cells to grow brain tissue("cerebral organoid")in the lab. The cerebral organoid is supposed to simulate life brain tissue. Hence research can be done on live human brain tissue without taking off brain tissue from a living human( which is unethical). Applications would be to study human brain development at the cellular and molecular level , developing medicines to treat diseases of the central nervous system and potentially regenerative medicine and "personalized medicine ". Hope my comment is helpful.

[@yashvibhat7162]

really great initiative! We need this science communication

[@jzeerod]

this is amazing! some one should take this video, then do it on a budget. of course would have to buy the package. looks like you need a lot of stainless steel. air ventilation and cleaning scrubbers. various instruments and apparatuses, some mechanical items, heaters. disposables. then to a whole youtube segment on it! try starting a go-fund-me on this very very sensitive topic. the implications are enormous, ethically, a whole new area. can we should we?

[@Fanaz10]

@Iwontsaymyname Sodontaskagain lol, who cares

[@isaacyoung1868]

this is so cool! # of people who watched this, 26,000.. number of people who will actually get to make cerebral organoids.. my guess no more than 10..

[@comosediceesoo681]

Well 11

[@atomicblonde9667]

My son has a regulation lab where they prepare stem cells for many holistic health treatments. I sent him this

[@oompalumpus699]

Thank you for this video.

[@nicewater894]

very much needed, thanks!

[@ayazmuhannad8254]

Very good job

[@KiranShehnazKaur]

At time stamp 7:41 black spots can be observed in few of the organoids, what exactly is that? And why don't all organoids have that? Is it normal to observe that while genrating organoids?

[@STEMCELLTechnologies]

The black spots observed in some of the organoids generated with our STEMdiff™ Cerebral Organoid Kit are attributed to retinal pigment epithelium which was reported in the Lancaster et al., 2013 publication (pubmed.ncbi.nlm.nih.gov/23995685/) our kit is based on. This is in alignment with the dorsal anterior characteristics of the organoids. It is common to see pigment in the organoids, however, this varies from cell line to cell line and they arise spontaneously within the organoids. If you have any more questions, feel free to email us at [e-mail].

[@hiraltalati440]

How to measure the diameter of formed Embryoid bodies or Cerebral organoid?

[@STEMCELLTechnologies]

EB/Organoid size may be measured by fitting the microscope with a calibrated ocular micrometer or stage micrometer. Alternatively, if images are captured using a microscope camera, a stage graticule may be used to measure the field of view. Some cameras and software will also automatically extract and provide magnification and scale bar measurement data. If you have more questions, please email [e-mail] and we'll be happy to assist!

[@carlfreer8600]

One of the most comprehensive explanatory videos. Thank you

[@gara5318]

Use 3D printed Biopolymer scaffolding in the correct anatomical arrangements so that entire central and peripheral nervous systems will formulate.

[@Perfectslim7days]

can copy our own brain with this TECHNIQUE?

[@bruno9005]

@@Perfectslim7days not exactly

[@hanumakumar8997]

Very nice explanation. Thank you.

[@cccl457]

Very nice explanation. Thanks.

[@zakirzak1494]

Very informative , but the background music is loud and annoying . Plz avoid it or make it minimal ,thanks

[@STEMCELLTechnologies]

Thank you and we'll share this feedback with our team.

[@maheshbhanushali1082]

Very much needed. Thanks a lot, can this be used for neurological disorders

[@rocklobster1976]

Where can I buy the whole kit.... and a pcr, thermocycler Oh.. and a copy of this track

[@STEMCELLTechnologies]

Hi, thank you for your interest in our products! Please visit www.stemcell.com/contact-us.

[@hiraltalati440]

How to conclude that formed Embryoid bodies are appropriate so as to avoid mistakes at very step?

[@STEMCELLTechnologies]

Embryoid bodies should be round, smooth, and symmetrical, with the edges appearing brighter than the centers, as shown in Figure 1 of our Scientific Poster (bit.ly/2EegQJz). There should not be any budding of the surface or amorphous outgrowth during the EB formation or induction stages. Formation of a few additional small EBs in the well is normal, as long as there is also one large, central EB with the characteristics listed above. If you have more questions, please email [e-mail] and we'll be happy to assist!

[@ashm5576]

Imagine how long it took scientists to figure out this process

[@shiwalikaushal9718]

How did you make the stem cell to make only the cerebral cells, aren't totipotent stem cells capable of making the whole body.. sorry if I'm wrong i haven't studied about this much just curious 😬

[@STEMCELLTechnologies]

Hi Shiwali, totipotent cells can indeed form all the different cell types found in the body, plus the extraembryonic, or placental, cells. Embryonic cells within the first couple of cell divisions after fertilization are the only cells that are totipotent. The cell culture media used in this protocol has been optimized to provide the ingredients and messages to ensure the human pluripotent cells differentiate to cerebral organoids rather than other cell types. If you have any more questions, feel free to email us at [e-mail]!

[@hiraltalati440]

How can one demark Neural rosettes from Embryoid bodies apart from morphology?

[@STEMCELLTechnologies]

Rosettes may be identified by immunostaining for neural progenitor markers such as PAX6 and SOX2. They should be visible as radialized structures within mature organoids, as depicted in Figure 3 of our Scientific Poster (bit.ly/2EegQJz). Our Technical Bulletin outlines a detailed protocol for performing this staining (bit.ly/3l0JZZj). If you have more questions, please email [e-mail] and we'll be happy to assist!

[@gara5318]

Use 3D printed Biopolymer scaffolding in the correct anatomical arrangements so that central and peripheral nervous systems can formulate.