How To Grow Mushrooms Part Two: Sectoring

[@badmandansanchez1823]

Super neat. My question is about the neat tools you have to do this. It does not appear that you cleanse them between stuff. I kinda skipped around so perhaps you explained that, but it looks a lot like your punching multiple dishes without sanitizing. I'm trying to figure this out. Petri dishes are neat

[@TroubleSome-ft9kv]

I appreciate the awesome vids ✌️😁☮️ Love the scalpel, I just went to your site and picked one up with a extra pack of blades. Love the content ☮️🍄☮️

[@TheMenezees]

Thanks for the video!

[@tysonpekarek8218]

Great video! Super informative! Thank you for your time and efforts :)

[@jamestavernier6182]

Good stuff. Thank you for sharing.

[@PickingFruits]

Thanks for watching!!

[@fernetstock1]

What agar substrate are you using? I guess you could make it thinner, more economic also the puncture cakes would less likely tip over. I discovered similar transfer method myself using sterile pasteure pipette cut slant to puncture but the relese mechanism is not perfect, pressing the balloon shoots it out often too suddenly and the cake is bouncing away.

[@PickingFruits]

Hello, I am not exactly sure what you mean by agar substrate, but what I use is agar powder. I have tried thinner pours in the past, but they dry out too quickly, so I prefer a standard thickness. The pipette idea sounds interesting, if I ever have some around ill give it a try!

[@fernetstock1]

@@PickingFruits Thanx for your answer and for your great videos, I've learned and 'm going to learn a lot in future I have the feeling. I mean what nutritients do you use for agar, pepton, yeast extract, malt extract, antibiotics maybe?

[@PickingFruits]

@@fernetstock1 these plates were all LMY. we don't use antibiotics, instead we use charcoal. As far as peptone, we don't use it either. We only use animal based products, (soon we will be trying soya peptone) so we just change up the recipe from grain water, to PD, to water back to LME. If you keep tricking your mycelium it will be harder for it to experience senescence.

[@PlasmaOne]

Doesn't the mycelium grow along the top of the agar? Would simply scraping off a section and transferring only the mycelium damage it too much?

[@ghostofaman4984]

This is a brilliant question. I pondered it for a while. He’s more trying to have the best genetics over general transfer. You could cut a “wedge” or so, but the method is proper for preserving the agar for future mycelium growth and transfer. If you’re just trying to grow; you’d use all the growth and transfer to bulk. Hope this is what you were asking and I didn’t over think it