The Agar Files - Inoculating Agar to Grain

Today's class will go over your colonized agar dishes and putting them in grain jars in order to make grain spawn.
⬙ PGT Links ⬙
🧫Links to all products/gear I use: kit.co/PhillyG...
(Using these links will help sensei earn a small commission and support the channel)
👕Merch Shop: teespring.com/...
📱Instagram: / phillygoldenteacher
📄Patreon: / phillygoldenteacher
💻Discord: / discord
⚪️Microppose Link for Injection Ports, use code PGT for a 10% discount.
microppose.com...
💉Looking for Spores? Use code PGT for a discount here.
inoculatethewo...
🦠DrMyc Products
www.drmyc.com?sca_ref=1761105.0L3Ag7LnMw Use code PGT for a discount here.
🌿Fungstrate
fungstrateco.c... Use code PGT for 5% off subs and grains.
🌱BoomerShroomer Inflatable Tubs
boomershroomer... Use code PGT for discount here.
🍄MushroomSupplies
mushroom-suppl... Use code PGT for 10% off.
Vivosun - Code PGT gets you 12% off.
bit.ly/3Fqa1BD
10z Ultra Fine Mister
amzn.to/2Io5apI
Glass Mason Jars
amzn.to/3lUTKaH
Brown Rice
amzn.to/36ZRNWu
Mirro Pressure Cooker
amzn.to/39QVMqb
Coco Coir
amzn.to/36VGUEQ
Vermiculite
amzn.to/3gu7qbS
Gypsum
amzn.to/3oCIVvY
Presto Dehydrator
amzn.to/39SL42q
(Using these links will help me out with a minor commission at no cost to you, thanks for the support)

[@matthewrick]

Sensei! You’re the only channel on KZbin that actually gets me amped for a premier! Thanks for the quality content!

[@naradax2364]

Same here

[@ezranator123]

Yo so true

[@connorhunter9394]

What do you do with the bird seed is it just raw from the bag or are they soaked or what ?

[@thirteen60oyvey]

Growing mushrooms is very satisfying. I'm about a month into my journey. It all started by being blessed with some fresh fungi n I made my first spore prints. Now I've just inoculated my first agar dishes! With a little more confidence I hope to be documenting and creating content to add to the interwebs, ty for the inspiration sensei!!

[@phantomhawk01]

Sounds awesome, I'm curious how did you come across fresh mushrooms and do you know what species is it?

[@zach1776]

Gopher it!!!

[@derekshrewsbury9539]

Are you making content yet?

[@DJ_Stroodle]

This is the most wholesome place on the internet right now, period. ✌🏻🍄 Thank you sensei!

[@Rain-di2bq]

I was literally just on reddit where someone had an amazing flush using your methods, outstanding!

[@dethyl]

Methods he read on shroomery****

[@BBCforyoHoe]

@@dethyl the real OG’s

[@michaelevans6216]

@@dethyl whats shroomery?

[@dethyl]

@@michaelevans6216 its a fourum site for information pertaining to cultivation of mushrooms

[@JayP7.62]

Spawned my agar to grain 9 days ago and they're colonizing FAST! Was already about 25% at day 7 so I gave them a very good shake and now a couple days after the shake I have mycelium growing from all over the grains. I didn't even do any transfers, but I did have lots of rhyzomorphic growth straight from the start. Went from MSS to agar. And I had almost gave up after two failed attempts at putting MSS straight to grain. Agar is the way to go! I have more on the way and will be doing transfers next, can't wait. I'm hooked on this hobby now!! Thank you so much for your videos! If it wasn't for you, home mycology and willymyco I wouldn't have given this hobby a try! Yall three are my top three channels for mycology info!

[@remingtonfrog3170]

So you unscrew the jars a tad & leave them; with temperature controlled space? Thanks, this will be my first attempt and I’m stuck with Temperature issues. Small space & on a budget 🙃…. Any possible ideas or suggestions for Temperature ? A small space heater 🤔😅🍄🩷🙏🏻

[@JayP7.62]

@@remingtonfrog3170 I use modified jar lids with the little stick on filters. Or just drill the hole about the size of a pencil and use two layer of micropore tape. As far as temperature low to mid 70’s. I use a cabinet in my kitchen next to the refrigerator. The fridge puts off a little heat and the back of that cabinet is a wall and on the other side of the wall is my garage. So I get heat from there also. Usually right at 75F there.

[@Supremebeing47]

Did you use a laminar flow hood when moving agar to grain?wondering if it’s necessary

[@JayP7.62]

@@Supremebeing47 Still air box.

[@FunGuyFruits]

I love it! I tell everyone you need to get into agar. Its crucial for mycology absolutely crucial

[@PhillyGoldenTeacher]

and it's a lot of fun too

[@JayP7.62]

For sure! I almost gave up after two failed attempts putting MSS straight to grain. So glad I gave agar a try. Will be using agar every time from now on.

[@michaelpoffenberger2482]

Man, I love what you're doing with educating everyone and helping people find a new hobby! I'm glad I found your channel back when you only had 3 episodes out. Mush love homie 💙

[@PhillyGoldenTeacher]

Thanks for being an OG!

[@kaneraymond6711]

Yo i had no idea this channel is less than 6 months old......dude holy shit, i thought this was maybe a channel built up over 2 years maybe, by far the best mushroom channel on yt, ur amazing keep it up teach!

[@PhillyGoldenTeacher]

Thanks! I appreciate it. I put in a lot of effort on my videos 😃

[@NBA-LejonBrames]

@@PhillyGoldenTeacher yea we can tell bro. Thanks . Spread that knowledge!

[@BlakeFlud]

Woohooo!! So thankful for you and everything you do for the community. Thank you PGT! 👽🙏

[@sin_mintz8299]

Love waking up to find out that I missed class lulz love the series

[@prestonkorey6799]

Even now that I've gotten this down , I still come back and enjoy this time and time again . Great content , thanks for doing what you do.

[@martintrevino6688]

Your the reason my first grow is going so well. Thanks PGT, you’re necessary for the mycology community

[@thecakeredux]

Did this today for the first time. Pleurotus pulmonarius that I cloned to agar from a wild specimen I foraged, six perfectly clean cultures (even smelling wonderfully mushroomy) into three jars of popcorn. I'm so excited. Thank you for opening up this endeavor to me through your knowledge and efforts.

[@cookieboy7102]

“Yayyyy” haha always love the endings

[@philipernst9515]

Your advice has helped me a lot, especially on fruiting conditions, got 60g dried out of a 1200 cc tub in 4 flushes. When I move to a new place ill start doing agar, thanks to your videos. You are a great teacher and I cannot wait for more videos!

[@samocupcakes]

Help! I keep getting wet contam, what was your process?

[@adrianjerry3652]

Quality content. Straight and to the point, no fluff. Keep it up chief. 👍

[@beckleyland1033]

The sound of you shaking the jar sped up, certainly got my cats attention, shes currently hunting the sound of your jar. Keep up the Good work

[@PhillyGoldenTeacher]

Lol thats funny, my cat gets startled when I shake my jars.

[@JayWes580]

Very chill yet informative vid, thanks man 🍄

[@danielable1676]

I just watched all your videos and they are awesome. Super informative and very soothing. Top notch! Really interested in learning about liquid cultures too so hopefully will see a video in the future from you!

[@PhillyGoldenTeacher]

Liquid culture is coming next month. I'm still testing out some recipes. Want the best for you guys.

[@agnidas5816]

agar to grain was my fav method back in the day :) . But I had a good hermetically sealed glovebox. I would drop one chunk per jar. I found that worked faster than spores anyway.

[@buildlittlebig8070]

PGT!!! Needed this vid for my first APE adventure I’m about to endeavor on. Perfect timing. Keep posting man your channel is the best out there and your knowledge is invaluable. Mush love!

[@markchick634]

Best channel on getting started on Shrooms.They have changed my life and after a car wreck Severe Brain trauma My Life was a Mess.I wasn't into doing mushrooms but with all the nerve damage and Meds the docs had Me on was Crazy.Not helping at all. For 3 months now I fruited my shrooms and been micro dosing 4 on 3 off. Amazing what it has done. Thank You for saving my life.

[@samy92879]

Hey Mark where did you get your starter kit. been trying to find some but they're all in europe. any suggestions

[@markchick634]

@@samy92879 I don't understand the question Kit?I got everything to do it which is very expensive for real.I mean by the time you know it one has 1000 dollars into just basic stuff.Oder Spores syringe tubs Substrate torch scales knives vermiculite gypsum,coir, alcohol papertowels,I mean everything.making a still air box is 200 in itself jars grain lids.

[@markchick634]

@@samy92879 Try Canada Colorado,Oregon to name a few plaCES that have all one needs to get started.Shroom Supply,Quality Spores is a couple places I use.

[@markchick634]

@@samy92879 No kit order spores and start.

[@kerimacgill7489]

Lovin it PGT! Gonna get spores as well as clones going on some agar in the next week or two. Great lessons!

[@bensheppard8182]

I've learned more from your videos than most all of the others combined. Love the way you explain things and make it super simple to follow!! Thank you for what you do, keep them coming.

[@brianpotter2135]

Always pleased to get a notification about a new video of yours, you never disappoint 👍

[@simonzowie]

Thank you for the content! I have learned so much from your videos , and wish you ever expanding success!

[@roci4209]

Thanks so much PGT, I found my love for mycology with you.

[@txanghellictx7283]

Everytime I watch one of your videos I always have something new to learn or take from it , and is ways explained in a manor I feel must can understand or learn from. Great video once again 👏

[@joseluis7118]

Basic, simple and easy to follow content. Great Channel for this topic.

[@kymfarmer4305]

Hey there! I am new to this but utterly obsessed, you are such a wonderful source and your voice so soothing! Thank you 🥰🍄🙏

[@lga_8885]

Thanks a lot for your dedication, your passion and all your Mycology clases PGT ! I'm honored to learn all this knowledge from you and sponsored by amazing people! 🙏✨🍄

[@aw6743]

I just got my hands on a spore print and instantly found your channel. Have been passively looking for spores for a year or so, and now there's your video series. If this isn't cosmic alignment idk what is. Thank you for your work! A question: when working with prints, would simply using a burner to work underneath (instead of still air box) be sufficient? I fear it's only useful in Lab conditions, where inherent pollution is very low from the start, compared to the multiverse of things circulating around in my room. Thanks again, and peace with you!

[@PhillyGoldenTeacher]

Still air box is best option unless you have a laminar flowhood.

[@jaimedelgado7529]

YO PGT .... *please* do a SAB tek. do's and dont's etc. I'm getting way too much contams on agar. gotta be doing something wrong here. my SAB is quite small as well. very frustrating

[@belahu]

Beautiful! So clear and easy to follow. Keep up the great work for all of us out there PGT!

[@turbo558]

*Zero thumbs down* Me: Damn right

[@FrontseatLearner]

Hell yeah! Love the content man, truly one of the best shroom KZbinrs out there!🤙🏻 Keep up the great work

[@682logan]

please keep making content T.T this is beautiful, thank you PGT!

[@plainlogic]

Thanks brother! It's working better than I ever expected! You've been a wealth of knowledge and you've helped me immensely.

[@PhillyGoldenTeacher]

I'm glad to hear of your success! Wish you the best on your journey! 🙏🍄

[@plainlogic]

@harrisonvictor4916 it's laced with fentanyl

[@beverleygreenaway4514]

I am new to learning how to grow Mushrooms. I have watched many videos on this on KZbin, all been amazing, until yours popped up. The way you explain everything in each of your videos, I find sO easy to understand. I have learnt so much in such a short time. Your videos are brilliant. Thank you for sharing your work and knowledge. P.S I have subscribed to your channel.

[@kerino1]

Sensei - you're the best! Thanks so much!!!!

[@jesschambers6551]

Thank you for all of the knowledge you share! 1st time I went with brf cakes and have been regretting it ever sense i saw your video on bods unmodified shoebox. I just knocked up 5 jars last night using broke boi tek. This process seems much better imo. Much love PGT! Canr wait for the next video!

[@robinhoode9364]

Another great content creator in the community. Thanks for the great video and useful information!

[@IndiaMikePapa]

What's the next step after grains? I could not find a video in your profile.

[@tannerdouglas2625]

Just about to try this for the first time, your excellent content is much appreciated!

[@03obietrice]

these videos are an excellent resource. much love!

[@PhillyGoldenTeacher]

Mush love 🍄

[@bakelietebakfiets3688]

Does loosening up the lid for gass exchange cause a risk for contaminatoin? Love your videos. Very to the point information

[@jesshuber2050]

contam isn't good at turning corners

[@hopperthemarxist8533]

This looks so much cleaner and easier than making syringe needles thanks b

[@Lagger-HD]

looks clean, didn't realize it was this simple, I just need a instant pot

[@naradax2364]

What a qualitative content ! As always in PGTs class ! 🙏

[@onigashima6603]

Thank you fro the hard work and dedication sensei

[@mikehoncho2149]

Hell yeah! Just gotta wait 11 hours, see y'all then

[@lonewretch]

_3 months laiterr_ :o

[@kicknadeadcat]

I have 8 different mushroom varieties at different stages growing.

[@seancostello26]

im up to 4 myself right now :)

[@NightWolf-vv5me]

Can you store agar w/ mycelium growth at room temperature for long periods? What is a good way to store it?

[@Dmyra]

"You can never be too careful, Sometimes" el PGT

[@lonewretch]

Haha I caught that too :)

[@smile-xo8jl]

Thank you from morocco.god bless you

[@ericabarnes9359]

Can you do a video on the bird seed jars?

[@Jess.moont2]

thanks for caption because i'm brazilian is i know i'll learn a lot from you

[@crktritual]

Dang....that Agar took off, especially compared to spore inoculation.

[@shockakhan]

I'm days from taking the first leap and getting my feet wet. I have most of the supplies, trying to decide what grain to use...

[@phantomhawk01]

I'm excited for you, I made that leap too now just getting ready to put my colonised jars to bulk sub, 😁

[@JayP7.62]

If you don't have a pressure cooker yet, or just want to get a fast start with pre sterilized grains. I used the 5 grain jars from midwestgrowkits and am having awesome results. Looks like a wild bird seed mix but doesn't have sunflower seeds or corn in it and it does have some rye grains in it.

[@FungalNetwork]

I would imagine any nice whole grain that isn’t too small (like quinoa).The pre sterilized bags I got off of north spore just to get going for the first time was millet I believe and it’s been working good so far.

[@niklashultkrantz7766]

I'm trying different grains. I got corn, wheat, sorghum and millet. Apparently rye is the best but they didn't have that in the animal food store.

[@JayP7.62]

@@niklashultkrantz7766 Actually millet is best. Smaller, more inoculation points.

[@denniswinters5883]

Ok im gonna ask a stupid question. After i put the agar in the grain jar does it need to be placed in a dark place or just regular light from a room or window ?

[@jham33333]

can you put those new plastic lids in the pressure cooker?

[@MyTube4Utoo]

Very good tutorials. Thanks.

[@ivanrico961]

At 1:40 when you said the amount you put will influence the amount of time it takes for the agar to be fully colonized. If I put more agar in the cup will I have a shorter or longer colonization time???

[@stephenclowes7216]

gday mate can u do a video with agar dishes on wheres the best place to sit em to speed up or generate best ideal spawn , cheers mate

[@erikbarajas8499]

Hey sensei I love the videos. I was wondering if you would ever consider making videos on how to make and sterilize agar and how to use a teK WITH a pressure cooker. Keep it up brotha

[@typerk]

That fluffy snow mycelium is beautiful

[@Mr.Gandy.]

Danke.

[@MM-uv3su]

You are a blessing

[@joeafkjr5021]

Hey buddy i just grew my first golden teather using your awsome Skills they turned off very nice but i think that i have some potency problems my friend took 1.5gram and had a mild effect when i was younger 1.5gram of mexican blew my soks off what am i doing wrong is it the way i try them or just normal for this strain hope you can help me by the way love your Channel bedst regards Joe 🙏🙏🙏

[@uriallon]

Thanks for the video! I always learn new things from you. I read that it's possible to use dried mushrooms in ager and they will make mycelium, do you know if it'a true? and can you show us how?

[@aCleverFishingPun]

Did you pressure cook the jars with those lids on them?

[@derrickmcadoo3804]

Why choose inoculated agar over straight spores?

[@SarcasticExecution]

The 7 day growth shown at 4:53 is why you'd use inoculated agar. Growth from spores will be A LOT slower.

[@jonathanfortner7461]

Faster

[@mzdznugz7365]

Thanks for sharing 🙏🏽

[@303otto]

thx for all your videos :) Whats the name of your funny little plastic AGAR Dishes or how are they called?

[@jamestavernier6182]

Great content! Thanks PGT.

[@Dakota_rides_the_gain]

For A2G, do the jars need a filter or no? I see no filter on the lids. I’d like to start agar process with jars, would be nice if I could skip making a filter for the lids. Thank you

[@evok_trip_toke]

Haha my corn just finished in the pressure cooker (((: gonna let it sit overnight and innoc tomorrow

[@fish9468]

LOVE YOUR VIDS MAN

[@quagenxd]

so my question for you is, can't you just do a spore syringe to one of your agar cups? that way instead of using a full cc of liquid in a jar you can just maybe put a few drops into an agar cup and use that to colonize a quart jar?

[@quagenxd]

would one drop of liquid be enough to colonize a whole agar cup? or will i need to transfer to another agar dish to fully colonize. If one drop fully colonizes the top of the agar than id only need one drop of solution per quart jar. any info is great thanks.

[@NightWolf-vv5me]

I see you tighten the jar after allowing the gas release for a week or so first, Is it okay to keep the jar tightened after that for the remainder of the colonization?

[@holdonasecondamigo599]

Thank you sensei 🙏

[@patrickmanasco8772]

I did not expect the Agar to just fall out so easy

[@jorgenio11]

Beautiful!!!

[@MikeMcc-c8w]

Are you inoculating your agar wedges in front of a flow hood? I’m going to use a hepa filtered iphone work station for to minimize contamination. I’m growing Lions mane for personal use. It’s helped my cognition dramatically. It took a while to work but I’m eager to grow more. Thank you for your videos, teaching time and generosity.

[@pernyqvist5677]

Very educational and well made video. You unscrew the lids of the containers to exchange air. As the containers are not in front of a flew hood, are you not afraid of contamination? This is only a storage for the containers in room temperature?

[@GGg-fx2fn]

Thank you teacher I am in Canada, Montreal and I wonder where can I purchase lion’s mane spores to cultivate them at home

[@josephparker2614]

How do you avoid contam after innoculation with the lid loose ?

[@geekay101]

Thanks you for this clear information. May I ask, your lids do not have any vents, is this not necessary, and do the lids need to be tight or loose please.

[@rgon541tube]

Where did the agar chunks go when u shook that jar a week later? Did see any agar chunks in that jar. Maybe i Missed it

[@bc8620]

I'm surprised how dry those grains seem versus how well it works. Those jars sound like maracas!

[@RSmith312]

Great video, thank you sir!

[@anthonybowling131]

Absolutely awesome thank you

[@danjf1]

Great videos teach!

[@chemicallyenlightened7025]

I love your channel man:)

[@user-jn7nf6xu8l]

If I understand correctly, you do not put filters on jars with mycelium?

[@tiagopewpew6232]

letss gooo thanks for sharing the wisdom bra

[@rustysmith-kc6ys]

So you do you PC you grain with the lids on and tighten them and after adding agar, you don’t have to worry about any breather holes?

[@c.cllayne9981]

The hot scalpel doesn’t damage the mycelium?? Can you cool it down on a paper towel with rubbing alcohol on it?

[@xj11w2c7]

I have not been using enough agar. Thanks for the video

[@BRIKSFYOS]

Can I use bags instead of jars. I tend to buy mine pre made. How about using uncles Ben’s with agar

[@robotrader9490]

Can you take spoon of the “ready” jar and add to new jar to start up the process again? And how long is it good to store ? Thanks for your channel man great stuff

[@elijahantony4300]

he has another video on this topic. Its called grain 2 grain transfer.