How to Make Sterilized Rye Grain Spawn for Growing Mushrooms

[@ThatsNotSmoke]

Finally, someone to tell me how to sterilize my rye berries! Videos everywhere on how to do everything else but this. Seems this is the first most important step. Thank you!

[@SporenSprout]

No problem! I’m literally making a bunch of grain right now using this same method! Gonna be up all night lol 🍄

[@Mistral434]

And every element laid out in this vid is borderline necessary. except measuring. for that i go by divine intuition (guessing) I know because Ive messed up each of these steps at some point - soaking too long, not long enough, boiling too long, not laying grain out flat to dry evenly, letting it dry too much.

[@hermanv.b.376]

Although, touching all the grain to spread them out and dry them out kind of UNsterilizes them, no?

[@Shane_Kerr]

Guys... Spreading the grain out into a mushroom is a necessary step. This promotes a emotional connection with the grain and provides happy grains, which in turn promotes happy mycelium. DO NOT FORGET THIS STEP!

[@brianreba6591]

What does that mean

[@sk8urbike935]

You will only understand at 2:50

[@gelhope5510]

Are the jar lids shut tight when pressure cooking?

[@Murphy_.]

Thats true. Every time I skip this step it's a failure.

[@DankMemer42013]

@@gelhope5510no, only close to where you first feel friction of the lid than stop

[@operationstayalive]

worked great, perfect moisture, very fast colonization, no contam. also love the towel dry method, makes gathering grain into piles so easy. thanks.

[@Wippersnapper88]

Back when we first started, there were no videos like this haha there was ONE video that definitely didn't detail anything. Thanks!

[@logancanada1826]

Haha you must not have known Roger rabbit then

[@jobbingactor]

Confirmed. Followed each step verbatim. Tested and perfect results using Golden Oyster LC.

[@Grimreaper420ful]

Amazing easy right to the point video 👑

[@SporenSprout]

Thanks for watching! 🍄

[@SethSongwriter]

Thank you 😊

[@SporenSprout]

Thanks for watching! 🍄

[@montygore1200]

I used whole wheat. It was 1/4 colonized in 24 hours. Wild.

[@KraziJoker]

Thanks for the video... Just made my first and so far I'm seeing some cultivation. Bouta make some more jars

[@SporenSprout]

Good luck and thanks for the feedback!

[@amberroll9945]

So weird. I just received an order from you guys today, and this just popped up at the top of my videos 😂

[@loulou7606]

Great video. Informative and to the point. Thank you.

[@SporenSprout]

@@loulou7606 Thanks for watching! 🍄

[@CasperBenfatah]

i was high and fell asleep for 9 hours and left them out to drie for 10 hours instead of 30 minutes , how cooked am i?

[@SporenSprout]

You are fully cooked, the grain will be too dry. You could try to soak the for a couple minutes and dry again but I would probably start over

[@zero1957]

so I definitely need to buy a pressure cooker?

[@SporenSprout]

Yes

[@GsHops0328]

Can a ninja or instant pot work or it has to be one of those?

[@SporenSprout]

@@GsHops0328 it can work but at 12 psi you will have to cook a little longer.

[@GsHops0328]

@@SporenSprout awesome. Thanks for the prompt reply. I appreciate it. Do you by chance know how much longer or at what time I should set it at?

[@merzhoykin]

hmm so bursted grains can cause contamination? I wonder if that was the reason behind both of my bags had to be thrown out.

[@SporenSprout]

@@merzhoykin Yes and when touching the bag you have to be careful not to smush the grains, like when shaking and mixing. The best way to prevent burst grains is to boil water and then turn off the stove before adding the grain. Stir frequently for 10-20 minutes until soft but not burst. They come out perfect every time if you do that.

[@LeonPhythian69]

​@@SporenSproutso don't turn the heat back onto a simmer? Or just use the already created heat?

[@SporenSprout]

@@LeonPhythian69 Yea sometimes the low simmer is too much so I just turn it off completely but the water is still very hot which is good.

[@LeonPhythian69]

@@SporenSprout cheers man, another quick, I've heard of peeps popping gypsum and a touch of old coffee water (so already used to make a brew then re brewed coffee) in at the soak stage with Rye have you ever given this a whirl?

[@codysmith1279]

Thanks man. Good vid

[@monobatiz]

Thank you for all this lessons!!!

[@miked5580]

Great vid

[@SporenSprout]

Thanks for watching! 🙏

[@Baudiahd]

Hi, what tape did you use for the top of the jar? Mine melted in the pressure cooker. I used transpore

[@SporenSprout]

@@Baudiahd that’s the wrong tape. Use this: amzn.to/3NNTQl4

[@Emilianoo8]

Appreciate it man 💪🏽

[@SporenSprout]

Thanks for watching! 🍄

[@idodo500]

First thank you so much for thia tutorial Second, i think my cooker pressure highest psi is 13, that wiil also work?

[@SporenSprout]

Cook for 2 hours at 13 psi and it should be ok

[@sterher]

Awesome

[@Fourpey]

Hey really cool video! If I don't have a pressure cooker, how would you proceed? Can I just boil the jars as I would when canning pickled stuff?

[@SporenSprout]

You can try that but it is not recommended. Grain is highly nutritious and needs to be sterilized under pressure.

[@MN_Grow_Bro]

Did you try it? How'd it work out if so?

[@valentinat3790]

One more question! Is it ok to use a preserving machine, like those used to make jam?which is not a pressure cooker but a boiling one

[@SporenSprout]

If you use popcorn kernels instead of rye you can use the preserving machine. Rye needs pressure

[@g1itch3d]

once the mycelium grows in mason jar how would you get it out? I assume it would be solid like a brick. Would you break it up with your hands?

[@SporenSprout]

Yeah you can smack the jar on a bike tire or something to break up the grains. I use bags and those are easy to break up.

[@sfrie1]

I add gypsum 1/4 tsp per quart.

[@gwilcox123]

Your videos a great! Thanks so much for producing them. I purchased some of your cultures and I'm beginning to sterilize substrates for inoculation. One question: you don't add any water to the jars after drying the rye and before sterilization? Does the grain get back moisture in the autoclave? In the Stamets book they add 2/3 - 3/4 c. Water. But they also don't cook it before, so this may change things. Thanks for your work!

[@SporenSprout]

I don’t add any water after drying. All of the moisture the grain needs is absorbed during soaking and stirring in the near-boiling water. There are many different techniques but this one works great for me still.

[@gwilcox123]

@@SporenSprout If it worked for u I'm going to go ahead and try it. Thanks for the quick response!

[@darkcity925]

How do you keep the grains dry for cultures that colonize slowly? Wet rot eventually gets to mine before all the grains are colonized.

[@SporenSprout]

After straining the grains from the hot water the steam helps evaporate moisture while laying out to dry. I mix up the grains 3 times every 10 mins while they are drying on the table. Also it helps to use 4-5 ml of liquid culture or a whole agar plate full of mycelium cut into many small wedges to inoculate the grains.

[@DARKW1ZARD616]

Hey man rookie question here what size are those jars??

[@SporenSprout]

@@DARKW1ZARD616 They are quart jars

[@Shambo44]

How did you make those self healing injection ports on the jar lids?

[@SporenSprout]

@@Shambo44 How to Make Self Healing Injection Jar Lids for Growing Mushrooms kzbin.info/www/bejne/p4vbd2N9o8yoiLc

[@garyballard3484]

I think it's good to add coffee grounds to it too, save some old coffee grounds for a couple days and dumb those into your bucket along with your grains and gypsum, it will make a world of difference.

[@judyhowell7075]

How much gypsum

[@garyballard3484]

@@judyhowell7075 Just a pinch between your thumb and finger is all I used, I used the pellet type because I couldn't find powder anywhere and it worked great. I've heard it's not really precise so just a pinch or maybe a half a teaspoon something like that

[@ericz9856]

Can I skip the cooking process? just soak drain and pressure cooking?

[@SporenSprout]

You can try but the grains don’t seem to swell enough without the 10-20 min hot water soak.

[@ambercombs5346]

When you pressure cook the grain do you tighten the lid all the way or loose with foil ?

[@SporenSprout]

A little bit loose with foil.

[@jucerble633]

how do you dry i mean either in the sun or in the shade? please help

[@خيراتالارض-ص6د]

Hello I am from IraqI wish I could grow mushroomsHow do I make this liquid inside the bottle

[@SporenSprout]

kzbin.info/www/bejne/fIuzgJWjd7ZmgdE watch this video and you will know.

[@خيراتالارض-ص6د]

@@SporenSprout Thank you 🍄🍄🍄🍄

[@suasponte6002]

Well done.

[@5dvz460]

Hi there, can you store sterilised grain jars for a long period say a week or two ? If so, how ? Thanks :)

[@SporenSprout]

Yes store in a cold room, but the grains tend to dry out over time pretty fast so I would use before 7 days

[@5dvz460]

@@SporenSprout ok thanks, I’ve put them in a refrigerator so hopefully that works :))

[@gobblenater]

Any tips for sourcing bulk grain like you have? Ive seen a few places selling 50 lb bags but all are $60-$80 rather than $20

[@SporenSprout]

Call grain and feed stores, or grain elevators, make sure to ask for “rye berries” not “rye”. Or you can try using wild bird seed or oats.

[@richardeimantas893]

Hi there, how can I sterilise rye and then use it in a grow box instead of jars?

[@SporenSprout]

Not quite sure what you mean. You can use filter patch bags or jars for grain spawn.

[@sphyre99]

Hi. I love your stuff. Which mushrooms work best with this method?

[@SporenSprout]

Much appreciated. They all work best with this method! 😄

[@ThatsNotSmoke]

@SporenSprout you, that was my question as well!🙂

[@BadddDoggg-id4po]

EXCELLENT!

[@HaydenRandall-qh4fn]

Do you 100% need a pressure cooker to do this? Or are there any alternatives? :)

[@SporenSprout]

100% need a pressure cooker.

[@taylorneal5825]

I'd say you 98% need a pressure cooker.

[@whereintheworldisbrennan3363]

Why do you open the bag outside? Just wondering if that was on purpose or not lol

[@SporenSprout]

No reason other than the grains being dusty and I didn’t want to get dust everywhere inside the house.

[@whereintheworldisbrennan3363]

Thanks!!

[@mrowkay]

after that leave the jar in a dark place?

[@SporenSprout]

Yeah that’s fine. It’s not 100% necessary, indirect light is ok.

[@loopedinlove8]

Before putting the jars into the pressure cooker, do you have the lids on tightened all the way or just "finger tight" to let it breathe? I've heard both so I'm not sure, great video btw!!

[@SporenSprout]

I leave them a tad loose, it not a big deal if you forget. You can add an extra piece of micropore tape if it becomes blown out after pressure cooker.

[@valentinat3790]

If i haven't covered the lid with the aluminium foil when put to sterilize what should i expect??

[@SporenSprout]

Depends what kind of air filter you have in the jar lid. You don’t want water wetting micropore tape. Excess water entering the jar can also cause the moisture content to exceed normal levels.

[@thecoolest1980]

How much liquid culture should I use per jar?

[@SporenSprout]

2 ml

[@ziggysplace3858]

Is it normal for the rye grain jars to stink after pressure cooking?

[@SporenSprout]

Yeah it smells like cooked grain.

[@ziggysplace3858]

@@SporenSprout There's a little visible moisture in the jars that wasn't there before pc, is this normal as well? The filter on the lids were slightly wet, hoping that wasn't an issue. Thanks

[@SporenSprout]

@@ziggysplace3858 Yes that is normal, you should shake the jars to mix up the moisture right after pressure cooking. If there is still pooling water after shaking then that’s bad. Its good to use tinfoil to cover the jar lids but sometimes the filter still gets wet. I usually put the jar in front of the flow hood and let the filter dry if it got wet or just rip it off and put a couple new pieces of tape.

[@MostWantedOHA]

pretty damn good ! video right here .... !!! '''

[@SporenSprout]

Man I really appreciate that! 🙏🏽

[@devinoliver1376]

i couldnt tell but how do you know if your pot is at 15 PSI? do you have a gauge or is it high temp thing or hows that work

[@SporenSprout]

@@devinoliver1376 Yeah it has a gauge:amzn.to/3MuqVl6 The weight will also start jiggling consistently once it reaches 15 psi.

[@devinoliver1376]

@@SporenSproutappreciate you, also when pressure cooking, is it necessary to have the jars raised on a lil wire rack inside the pressurizer or can they sit on the bottom with the water. i assume the rack is necessary and they need to be elevated in the chamber but i just wanted to see if it mattered

[@SporenSprout]

@@devinoliver1376 Yeah the jars should be elevated off the bottom to prevent cracking the glass.

[@devinoliver1376]

@@SporenSproutthank you sir, and one more question, after 90 minutes in pressure cooker, do you remove lid and let cool? or do you leave lid on until everything is cooled down?

[@SporenSprout]

@@devinoliver1376 Let it cool down a bit.

[@mcdthedev]

Is there a way around stabilising jars since I don’t have a pressure cooker? Does boiling the jars in a pot work ?

[@user-jn7nf6xu8l]

tell me please, if the temperature is 121 degrees Celsius, will it be enough to sterilize in 90 minutes?

[@cedricboucherie437]

What the size of the jar fella?

[@SporenSprout]

Quart

[@brandonjohnson1665]

How much liquid spores do I need per jar?

[@SporenSprout]

2 ml is good

[@Leo-gg5nv]

Hey thanks for the video do you have any information on spawn grain to substrate ratios? I don't know how much grain to prepare? I have a huge mono tub its 35 inch long, 11 inch wide and will be 2.5 inch depth of my substrate. Im new and lost with this particular aspect. Thanks

[@SporenSprout]

I would do 4-6 quart jars of spawn in a mono tub

[@grantshort]

I thought we needed to add about 10% gypsum to the mix

[@SDNKado]

how much lb of substrate does this make?

[@ZeStaticWolf]

What happens if I forgot to boil the grain?

[@SporenSprout]

It may not be fully hydrated or the germinated endospores may not have been sufficiently destroyed.

[@raquiti]

Once your done with this can you take the jars out of the still air box and leave them anywhere? I was wondering if when using micropore tape you need to cover the lid with aluminium foil or if the micropore tape is enough. Thanks for the vid!

[@SporenSprout]

yup you can leave them in room temp or hotter. always use tin foil

[@toribowers9766]

About 5/10% of my rye grains split open during the process. I know now my temperature was too high. I’m picking out as much of the split ones as I can. Should I start over or do you think I’d be okay?

[@SporenSprout]

I usually pick out a hand full of bursted grains and it works fine so I think you will be ok. You should boil the water first and then shut off the stove completely and then dump the grains in while stirring frequently for 20-30 minutes. That should help cut down on bursted grains in the future. Sometimes while laying out the grains to dry, the steam will dry the grains too well so I recently started rinsing the cooked grains with cold water to eliminate the excess loss of moisture from steaming, this helps retain moisture if the grains become too dry over time.

[@toribowers9766]

@@SporenSprout thank you!!

[@vk081064]

​@@SporenSproutThat's a great tip while often missed. Thanks

[@luluhernandez1790]

I’m going to use this method but with brown rice because it’s cheaper in my area. I watched a lot of your videos and I’m ready to try growing some king oyster mushrooms. Thanks !😊

[@luluhernandez1790]

I see you have it on your website, nice

[@jakelooter5139]

rice is a junk grain spawn it is better ground into a powder and mixed with vermiculite. I have attempted using rice as grain spawn a million times, it is not good. I have made it work but it is more or less junk

[@jakelooter5139]

use millet

[@devildiaries6563]

Hey. Is it okay if preassure is 0.6Bar (under 1psi)

[@DS-xg9kf]

No

[@ZenAndPsychedelicHealingCenter]

Nope and do not take advice from this channel. It's using debunked, outdated ideas and practices.

[@raimondtracar8703]

On your cooker, do you have psi meter or how do you know if the pressure is 15 psi. And can you inaculate the jars with spawn plugs/dowels?

[@SporenSprout]

My new pressure cooker has a a psi gage, but some do not have one. There are two weights on the pressure cooker in this video that creates 15 lbs of pressure. No matter what kind of pressure cooker you use, you need to use 15 psi for 2.5 hours.

[@SporenSprout]

Yes you can do that.

[@raimondtracar8703]

Thanks man. Appreciate it! Good luck

[@SporenSprout]

@@raimondtracar8703 No problem! Good luck to you as well!

[@maaz1010]

@@SporenSproutwhat if we don’t know how much psi we are doing? Cause in my region the cookers dont have the psi gauges.

[@HasF.4kZ]

Dude why nobody says how to store stuff 😢 liquid culture thats colonizing or grain spawn

[@SporenSprout]

@@HasF.4kZ It’s as simple as asking a question. Liquid culture is stored on a shelf in a room that is 70-80 F while colonizing, once enough mycelium has grown the jars are moved to a. Cooler room 60-65 F to stay for up to 6 months. Grain spawn is basically the same, but should be used within 3 months.

[@HasF.4kZ]

@@SporenSprout thanks alot 👍🏻

[@DevoutJourney]

Hey brotha, quick question. So Inoculated brown rice grain spawn about 3 days ago with Lions Mane. 3 of the 5 jars are showing mycelium growth pretty strong spots and 2 of them I can’t see them doing anything. Is this normal? Temp between 63-70 degrees F, humidity between 80-90 RH

[@SporenSprout]

3 days is a very short period of time for lions mane. The fact that you are already seeing spots on a few of the jars is good. Give them more time (1-2 weeks). Also lions mane mycelium is very thin and hard to see at first.

[@DevoutJourney]

@@SporenSprout thanks brotha

[@flopilop3808]

Why brown rice specifically?

[@SporenSprout]

This is not brown rice, it is rye seed.

[@flopilop3808]

@@SporenSprout oh I know but a lot of people specify brown rice when talking about spawn media, why not white rice ?

[@SporenSprout]

@@flopilop3808 brown is just more nutritious.

[@dr.ragnar]

Of all the grain spawn preparation methods, I like yours the best. I have tweaked it a little by i.e. adding some gypsum. I then use large grain bags which after sterilization I inoculate with some Uncle Ben’s tek material. This multiplies the colonization (by 3-4x) which I then multiply further (by 2-3x) by adding the grain bags to coco coir + vermiculite monotubs. I really like and use the relevant bits of the revised Gordo-tek by Rick James kzbin.info/www/bejne/hp6Ud5p8e9-EmpYfeature=shared and Boomer Shroomer’s technique kzbin.info/www/bejne/hp6Ud5p8e9-EmpYfeature=shared. I tend to be liberal with the use of hydrogen peroxide during both the preparation and the growing processes and seldom get contamination. My own extra tweak is to use a small tanning lamp (Fiji Sun) which I turn on for 10 minutes 2x per day - I haven’t measured it but the growth seems faster and the mushrooms more potent with a little sunshine, even if just imitated ☀️🤓

[@LeonPhythian69]

Can I, ask you a quick question? How much more weight do you get from the grains once hydrated? And have to tried a little used coffee water as well as Gypsum in the soak? I got some pre prepped bags that had used this prep method and they went completely crazy super quick

[@saandychaleen7668]

Any one know where I scan get liquid culture psilocybe cubensis at bought spore syringes about four weeks ago and did everything and still no sign of colonization I did research and heard liquid culture or agar if faster and easier and I’d like to give it a go

[@SporenSprout]

Liquid culture is king. Try “high desert spores” he’s the man.

[@greghalfman7669]

I’m going rogue I boiled wheat berries dried them for a bit into a alcohol stabilized jar with a dry magic cap Will let you know what happens because it looks like you read everything Idk what to do if it works I got a bucket 🤷‍♂️

[@SporenSprout]

If you are not sterilizing the grains I recommend using a lot of mycelium to inoculate the grain. It will be a race against contamination. If the mycelium dominates the grain within 1 week it may have a chance. Good luck! 🍄

[@revltech]

Hi... I from Malaysia. Is that possible, sell me some mushrooms sprout prints.

[@SporenSprout]

Etsy.me/3fbMzsV

[@revltech]

Noted with thanks

[@Dangerouswildarachnids]

Great video but you could do with some sharper scissors lol cheers

[@infectedpuddle1638]

You forgot whole oats

[@TheCollapse410]

U truly don't need a hood like that or anything. I've never noticed a dif. Sometimes infection just happens.

[@tonyreptilesrfriends9329]

I don't get it. All that sterilisation, just to leave it out on contaminated towel only to put contaminated hands to leave it in contaminated air? That makes no sense

[@SporenSprout]

lol it’s sterilized after using the towel…

[@amosusunday2669]

what do you inject into the bottle

[@SporenSprout]

Liquid culture or spores