How to measure SEED AREA in imageJ| Area measurement| Tips on how to capture seed image for analysis

[@vishwanathiari]

Thank you, there are many videos on YT, this was very good and easiest.

[@AliceritaE]

You're welcome, I'm glad you like the video. Feel free to ask for more ☺️

[@MyI2016]

Very helpful 😸

[@AliceritaE]

I'm glad 😊

[@douglassancho2894]

Thank you very much for this video, it was very helpful. I have some questions. When I transformed my image into binary, in my case the seeds appeared white on a black background. I was able to follow the following steps Binary >Options > Unselected Black background, to have something similar to the video. But I was left with the doubt if working with a black or white background affects the analysis, or is it just a visual thing depending on the sample, or does it depend on the analysis that determines the background? Do you consider that it catches the eye faster the fact that the seeds are black or white? Thanks in advance.

[@AliceritaE]

You did it correctly. The black on white background or vice versa is just for aesthetics. ImageJ is able to read the 0 and 1s of the pixel.

[@nanditanegi1041]

Yours was the best video for seed area measurement. Can u upload video on seed shape analysis for different shapes. I am having the images. Can you guide me with it.

[@AliceritaE]

Hello, In the video, there is a part where I set measurements. You can add Shape description to the set measurement.

[@AnneBesantyaCarroline]

thank you. Btw is there any tips to fill the black part (in this case the area that we want to count) bcs sometimes it is less to the black part that we want to count.

[@AliceritaE]

Yes, there is a way. I will make a video and upload it in 3 hours time.

[@danheights]

Very informative video. The video provides valuable information that I intend to utilize for analyzing my rice grains. I have a question concerning the Specific Leaf Area (SLA). I am interested in measuring the SLA of the rice leaves; however, I am unsure about the appropriate amount of leaf sample (fresh weight) required to determine the area of the leaf sample (cm) in the SLA formula, which is defined as follows: Specific Leaf Area = SLA (cm/g) = Area of Leaf Sample (cm) / Dry Weight of Leaf Sample (g). Thank you

[@AliceritaE]

hi Dan, For the SLA calculation, my advice would be to do the following; 1. Cut a fixed length of the all freas leaf (assign numbers S/N to each leaf) 2. Using the S/N of each leaf, measure the area of each fresh leaf and document the values 3. Weigh the leaf to determine the weight It is important that you stick to the numbering of each leaf. so that you can get the correcty SLA for each individual leaf and then you can plot the distribution of the SLA in a chart or find the average SLA of all the leaves. cheers!

[@danheights]

@@AliceritaE Thanks Dear

[@AliceritaE]

You are welcome

[@danheights]

@@AliceritaE I would like to inquire about Stomata Analysis in Image J. Do you possess a protocol for this procedure? Specifically, I am interested in understanding how the initial leaf imprint image is obtained. Is the imprint achieved through the application of nail varnish, or is the image captured using a scanner or camera?

[@AliceritaE]

Hi Dach, you can capture the image of the leeeaf with a camera or scanner. all that is important is that you add a ruler beside the same before scanning or capturing the image

[@virtual.fruiting.chamber]

You are so cool. Thank you for the video

[@AliceritaE]

You're welcome 😊

[@FrankyScience]

Hi Alicerita! Your tutorial has been very useful for my passed and current work in lab! Do you have an instagram account where we can follow you?

[@AliceritaE]

Thank you for your kind comment. It made my evening. I'm on LinkedIn as Alice Eseola