⚖️ How to PREPARE SAMPLES FOR MASS SPECTROMETRY || Proteomics || Protein Analysis Via Mass Spec

[@adwoabiotech]

REFERENCE Protein Sample Prep for Mass Spectrometry: bit.ly/3n1yrX3

[@tylerfield6447]

Trypsin is used because it is robust (works at a wide temp range) and most importantly cuts specifically and reliably at the C termini of Lysine and Arginine residues. That specific cutting is important because the mass spec software data base of peptides is based on this type of cutting.

[@adwoabiotech]

Thanks for schooling us, Tyler!

[@tylerfield6447]

@@adwoabiotech Thanks for the great video!

[@sohaalali4339]

I assume Trp is a more reliable enzyme since it produces positively charged amino acids Hence, the chance will be higher for these amino acids to be detected.

[@sohaalali4339]

Thanks so much for your great presentation!=) I really enjoyed it. ^_^ Would you please also make a video for mass spect data analysis? Thank you!

[@adwoabiotech]

Thanks for your comment, Soha! Absolutely, I'll get on that in the new year (inshallah).

[@sohaalali4339]

@@adwoabiotech Thanks so much dear ^-^

[@sohaalali4339]

@@adwoabiotech Thanks so much dear ^-^

[@sohaalali4339]

@@adwoabiotech Thanks so much dear ^-^

[@bakhtnama8325]

Thanks

[@adwoabiotech]

Happy to help.