How to use a Soxhlet to Extract Mushrooms

[@ricomanuel7567]

I'm going through mushroom infusion therapy, and I can confirm that it does work real good..

[@morgancr1993]

You can get out of habits easier after a mushroom trip and get a different routine going, I couldnt even finish a cigarette afterwards because it almost made me throw up with how horrible it tasted.

[@johnnyherbaugh3804]

Can it help lungs and liver

[@amirziarati3663]

@@morgancr1993 چچچ

[@NotAVeryGoodSkater]

For cubensis mushrooms? I'm looking for some magic in my medicine.

[@coneman4978]

The vibe here is next level, actual chemistry explained well enough for non-chemists to understand. I'll be scouring this channel and watching any new content. Mush love 🍄

[@Fungiacademy]

Thanks Coneman! Appreciate it✌

[@KieranChallis]

Apart from the fact that he got a vital step wrong.....alcohol extraction shouldn't be first for most medicinal mushrooms which he would know if he understood the chemistry.

[@cognitivedissonancecamp6326]

Very generous dispensation of clear experience! I feel like I could do this tomorrow.

[@Fungiacademy]

You could!

[@Sto0v]

Good stuff! Handy tip: Use a pan with water to hang the flask in. And if the warmthbath needs to be higher than 100 degrees, C there's always oil you can use.

[@MedoKojiZiviOvde]

You can also use ether for the extraction. It boils at a much lower temperature and less likely to denature the compounds due to extended heating periods.

[@Fungiacademy]

Great tip!

[@WarlordEnthusiast]

Yea but then you need ether, good luck buying that and storing it can be quite dangerous.

[@MedoKojiZiviOvde]

@@WarlordEnthusiast You can make your own ether and if storage is an issue, you can make when you need it. Once the extraction done, you can let it evaporate and add ethanol

[@MaxG-jk8ty]

Ether is definitely something to be respected in a lab setting. My understanding is the reason it's "hard to get" is to deter accidents. There are also legality issues in many countries.

[@MedoKojiZiviOvde]

@@MaxG-jk8ty Correct as the peroxide formation in an old bottle can require a bomb squad for its removal. Making it, using it and getting rid of it in a short period is the safest way for average joe. Also, it was used a recreational drug.

[@GnarGreen]

Man this video is off the charts. Love the vibe and the knowledge. I’ve been looking for this option. Just didn’t know it existed. Thank you. Mush love!!

[@Fungiacademy]

Thanks man! Really appreciate it🙌 Mush Love🍄

[@Rawi888]

Very informative. Very positive energy, thank you for sharing.

[@Fungiacademy]

Appreciate it man!✌

[@Rawi888]

You guys are living my dream. I'm just here having trouble fruiting my stuff and here you guys doing alchemy 🥰

[@Fungiacademy]

You got this! Practice makes perfect :)

[@girrrrrrr2]

Where is the issue? Are you getting full colonization?

[@yrisolivera9490]

Holaaa!! Soy de Uruguay !! Recién coseché x primera vez hongos mágicos ❤ explícame como hago micro dosis 🙏🙏🙏 gracias gracias gracias

[@michaelgetman826]

I live in florida, water abound. I sill use a cheap $5 water pump in a 5 gallon bucket for my soxhlet coolant system. No reason to waste water regardless of where you are

[@dronelabs556]

How do you get it cold enough. Need extra cold for reduced pressure.

[@michaelcarey9359]

@@dronelabs556 Pressure? You just need a pump to keep it going through the condenser. A five gallon bucket is gonna get warm, fast, recirculating it. Liquid water really does not compress

[@dronelabs556]

@@michaelcarey9359 no the soxlet is under vacuum. Reduced pressure. So there is no air in the soxlet allowing me to boil solvents at lower temps or control the extraction temp. By controlling the vacuum. But I’m Just tired of switching out the warm water in the bucket.

[@dronelabs556]

@@michaelcarey9359 this guy figured it out. Cold battery with a cheap freezer. Instead of buying a chiller. kzbin.info/www/bejne/kGXZfI1umbB6qJYsi=3tf8D0qzXTqisDed

[@SporeWhore661]

​@@dronelabs556nitrogen tank

[@cobrakai8049]

I made my 1st batch of tincture last Fall. Used a method you showed 12 years ago. My tincture came out great!!! I would to try this set up.

[@cobrakai8049]

This turns my 2 month wait time into a 2 day wait!!!

[@cobrakai8049]

With my 1st round I did a 50/50 mix of alcohol to water. And I agree, it's to strong with alcohol. Makes it so I don't like taking it straight.

[@mateuszm980]

It's important to note that active compounds, such as polysaccharides and triterpenes, can degrade at specific temperatures. For example, polysaccharides may begin to break down at temperatures above 80-90°C (176-194°F), while triterpenes can degrade at temperatures above 50-60°C (122-140°F).

[@mskinetik]

Will this workd for the magical variety?

[@Shenwinchester]

This is what I want to know.

[@coralsihaya]

​@@ShenwinchesterMe too...following

[@AaronBaron-e7y]

I use a chiller set at -20c and pull a vacuum on the condenser as well. The vacuum lowers the vapor temputure using 190 proof ethanol and the chiller condenses the the vapor before the third bubble in the Allihn condenser

[@Fungiacademy]

Amazing!

[@MrForeverOnline]

Hi! Great video, was looking for something I can do @ home for a productive healthy hobby that is still relatively small scale. I was having a bit of trouble finding information on how and if it's possible to turn the tinctures into a powder through a dehydrating machine or something to cook off the alcohol & scrape up bits to capsule it? I rather take it as a capsule pill. Other methods I saw require using a freeze drying machine (2-3k) or vacuum extraction pump to remove the alcohol ($900-1K), a $60-$80 dehydrater sounds like a more affordable price range. Thanks again for your video!

[@griffintwalsh]

This videos is amazing. Small thing to edit, the Erlenmeyer flask amazon link goes to small chese cloth.

[@Fungiacademy]

Are they not the same thing?

[@yatuza]

Great Information man!! Thanksa lot!

[@Fungiacademy]

Happy you liked it!

[@smoothbrain8519]

Awesome love it, and thanks for providing the equipment list and links. Happy making!

[@Fungiacademy]

Our pleasure! Happy making to you :)

[@mike31593]

Have you heard about using an altrasonic to break the cell walls of the mushroom.

[@Fungiacademy]

Oh yea, we briefly mentioned it in our last video but the Ultrasonic cleaners aren't as effective as we thought. The real deal are these Hielscher machines but they go for $10k easily😅 Not really in our budget and doesn't seem applicable for everyone to have one of those in their homes just yet.

[@jake_runs_the_world]

amazing ! i run a mushroom company in india this really helps me to start selling awesome extracts !

[@Fungiacademy]

Keep it up! Spread that Fungal Love to India!

[@truealchemy-jayhanks2653]

Thank you for the vid!! I use this also for plants. Wild lettuce does an amazing soxhlet extract. Just started mushrooms. I was using the soxhlet with alcohol, but then doing a 4 day decoction with water, then adding the two together. I love this shit. I also make spagyric essences, so all of this equipment I already have. If you have the distillation equipment, you can distill any alcohol to 95% and use it for your solvent. I will do this with old tinctures or ones I think could be better. Great way to recycle!

[@Fungiacademy]

I wish we had a rotovap :(

[@Chris-no2li]

Thanks for this great video! I am running my first soxhlet extraction at the moment. I can`t find this rosin bags, your amazon link didn`t work. Can you give me an explanation it`s normally used for so that I can look for it here in Germany...Is it a tea bag? And my liquid running down is almost clear - has circled twice at the moment. Does this mean it`s ready? you explained abaut 40 circles till it`s clear..

[@KatieJones89]

Thanks for this inspiring video! Im wondering if you have you used this method to successfully extract psilocybin?

[@elbonbon914]

That’s what I wanna know myself I see nothing about it but might not be something they say 🤨

[@p_peja]

Thank you for this useful video. I have question regarding alcohol extraction. The alcohol boiling temperature is somewhere around 78°C when it is pure. During soxhlet extracting method all substances extracted with alcohol are "boiling" for a long period of time. Are they affected/degraded by this?

[@Fungiacademy]

It seems so, but there is a sweetspot around the 50 cycle mark where you don't lose that much of the constituents

[@neiljoy8465]

Great vid! Thank U for the shared knowledge😌🙏🏻 I actually found a cordycep up in the forest behind your place there.I wonder if you've been able to find them yourself and cultivate that local strain🤔

[@Fungiacademy]

You shouldve brought it over!

[@bm4845]

there are two types of condenser. Is this type that is in the video recommended? Thank You for sharing the knowledge. I love Your flow :) all the best for You

[@Fungiacademy]

Yea i love the bubble one myself

[@vazap8662]

That was a fantastic video, clear and super informative. I like your style, friendly, efficient and fun. Thanks!

[@Fungiacademy]

Awesome, thank you!

[@kim2796]

does it work to extract psilocybe ?

[@joesucher]

so it do not affect the extraction process if you do not press dry the satchel in between the alcohol and water extraction. Nor it affects the extraction process if you do the water or alcohol extraction first or second?

[@Fungiacademy]

I press mine a little bit but I don't think it matters. I do prefer using alcohol first before water for this reason

[@manshamakhija3701]

Hello i wanted yo ask for extraction of cordycepin using soxhelet what ratio of cordyceps and ethanol u used and in that ethanol did u even mixed water if yes than how much water can u yell please

[@ianwynne5483]

Hi there, really appreciate the video, it was a great demonstration of double extraction. Is there a way of removing the alcohol post extraction for those who cannot take alcohol, or is it possible to use citric acid or glycerin instead of alcohol to extract the same nutrients.

[@Fungiacademy]

yea you can cook it out, by leaving the tincture slightly heated. Glycerin will not work in the soxhlet tho

[@ianwynne5483]

Thanks.

[@HBARbarian]

Thank you for sharing! I must've missed how you ended up with more water than alcohol. Did you fill the flask to 750ml for both solvents? If so, was the difference in final volume due to the 5 hour cycle time for alcohol and 3-4 hour cycle time for water? I don't want mine ending up with a 50/50 mixture and like your idea of 1/3 alcohol to water😅

[@Fungiacademy]

exactly, just add less alcohol to the flask. 500ml seems to be the minimum but I lose like 150ml in the process

[@ronenbarr3896]

Can you advise on 'correct tubing'? Thanks!

[@Fungiacademy]

Do it, I guess

[@ronenbarr3896]

​@@Fungiacademy Which type of tubing you recommend, specification?

[@griffintwalsh]

Wonderful video! Just wondering are you drying out things like lions mane before grinding for a tincture?

[@Fungiacademy]

Oh yea, always make sure the mushrooms you are planning to extract are fully dehydrated, for why check our other video on mushroom tinctures 👉 kzbin.info/www/bejne/p4Xak4aYesqhrLM&t

[@Piedra302]

Great videos and great channel!! Can I ask, what kind of bags are those that youre putting the mushrooms in? What are they actually made of? Also what total volume (plant:liquid) do you recommend for the most potent? I just made a 1:1 spagyric which I think may be way too strong lol

[@Fungiacademy]

Resin bags they're called. You can also make or buy small cheese cloths or tea bags

[@profesao2000]

The whole reason I was interested in getting a soxhlet was to make my home month long double extraction into something closer to a gram per milliliter. Your ratios are still 1:5-1:7, depending on what kind of mushroom, which means it will take at least 5 mL, or full droppers in a tincture, to make it equal to 1 g of dry. Do I need to get a bigger soxhlet than you or just replace the bags like 6 times which seems like will take a while?..

[@Fungiacademy]

People like to get bigger soxhlets, altho ratios might be similar the product is more potent after soxhlet extraction in my experience

[@profesao2000]

⁠ I understand it may be stronger, but I need to put down how many milligrams a milliliter is on my labels. I am trying to get a 1:1 with a ml equal to a gram of mushrooms. So I would actually have to make it stronger than that with the alcohol because I’m going to have to introduce twice the amount of water.

[@amitbarikeri3]

@@profesao2000, hey did you get ur ratio to 1:1 ? What proportions did you go with ?

[@GooseyPrime]

Awesome educational and entertaining video! Thank you!!

[@Fungiacademy]

Really appreciate the kind words🙏

[@mamafunky]

thank you very much, i have a question: what if i use the final extract again in another extraction soxhlet for more concentration? can i do that?

[@Fungiacademy]

For sure! i do cover the multiple cycle idea in the video but make sure not to overdo it since youll be exposing your extract to too much heat

[@mamafunky]

​@@Fungiacademythank you so much, you're so kind and generous. Greetings from Chile

[@GIANMANSUPER]

6:36 blue tincture spotted, what you extracting??? ;)

[@Fungiacademy]

😏

[@senseialexsprfk]

Have a couple of questions if you could be so kind as to enlighten me. Where can I find quality extracts if I'm not to do it myself? Would you recommend a blend of mushroom extracts or buying each one individually? Would you lose potency and effectiveness with blends? Thank you for your time and great content!

[@Fungiacademy]

Each one individually for sure, you'd def lose potency with blends. Check out Mindfull Mycology

[@gollgmias8666]

Very nice follow up Video! Thanks! Did you get your extracts tested for compounds by a laboratory? I tested your previous method with the ultrasonic cleaner but wasnt satisfied aswell. This Method seems more promising. Greetings and love from Austria!

[@Fungiacademy]

No, we're in Guatemala unfortunately so testing is really difficult. It feels better tho! Let me know if you get to test it and what the results are!

[@gollgmias8666]

@@Fungiacademy Thanks for the reply, keep you updated.

[@caden4898]

I use a ultrasonic cleaner to do my alcohol extracts. Is there a benefit to using the soxhlet method over ultrasound or does it just look a lot cooler? Thanks for the tutorial!

[@Fungiacademy]

Ultrasonic cleaners aren't the best in my opinion, all mushrooms require different frequencies so you need to find the sweetspot

[@ManaMotionFilmproduktion]

Looks great! But isn't Cordycepin and many other compounds that you would want to have in your extract breaking down at this high temperatures?

[@Fungiacademy]

I think the opinions are divided on this topic. I know plenty of organizations blasting their product with heat and still get great test results from the lab

[@bmartin2304]

Thank you for the info! Is it possible to process this further and get all the alcohol out and make a resin style extract? Also a good tip for the people watching this, If you have a good food processor it works great for powdering the dehydrated mushrooms in a fairly decent volume quickly! I have the cheap, mini kitchenaid and it works great.

[@Fungiacademy]

Yea, you'd need a rotovap for making a resin style extract. Food processors are expensive compared to the spice grinder and they tend to break when trying to blend high chitinous mushrooms like reishi or turkey tail

[@bmartin2304]

@@Fungiacademy Ok good to know, I haven't had the issue with breaking and my food processor is 4 times cheaper then that grinder which seems painfully inefficient by comparison. the fact you have to run it for 5 mins in such small quantity seems like a waste of time.

[@joesucher]

what are the lab tests on the extract called? to test the process.

[@Fungiacademy]

HPLC generally. Some people use Mas Spec

[@reaganadams379]

A video with your sonication method would be great.

[@AllenReza]

what adaptor are you using to connect from the water faucet to the condenser? ....very cool video. Thanks!

[@Fungiacademy]

Just something we yangled together, would probably be different for whatever faucet you have

[@jasne2992]

Does the extract get its dark color from heating it up, maybe? And what are the benefits of this method compared to just doing it in jars, and wait 2 weeks? (Without all the labor and electricity costs?)

[@AlteredVibez]

6:37 What size and micron rosin bag are you using? The linked ones seem too small.

[@chadbussie]

i purchased the 120 micron ones from the link. they are all 2" x 4.5". The 1st cycle produced a little bit of sediment.2nd cycle was pretty clean. also 70g of cordyceps fits in 3 bags and my chamber will only fit 2 so i have to run an additional swap. 6 bags total. Hope that helps. i may look into getting so bigger bags

[@Fungiacademy]

I forgot🙈

[@mshore74]

Is there a reason you need to use a soxholet for the water extraction? I was thinking that just using a pressure cooker and the amount of water would work very well and be an easier second extraction for the home extractor. Also, how is the potency of the soxhlet extraction compared to a standard tincture extraction?

[@Fungiacademy]

Pressure cooker would probably be too hot but a slowcooker would work. Personally I think the siphoning helps 'pull' out the alkaloids and makes it more potent

[@pablog.3906]

Nice apparatus, but the delicate compounds have to unecessary stay at high temperature during 5 hours. I guess cold extraction with constant agitation/vibration might get some extra sensitive molecules.

[@Fungiacademy]

Yea absolutely right, altho test results show Soxhlet extraction to be quite potent

[@deepnineforty5646]

Where do you sell your tinctures

[@Fungiacademy]

Keep your eyes peeled👀

[@deepnineforty5646]

@@Fungiacademy - I found some on amazon. thank you

[@Raye-ml1hx]

I got my stuffs delivered discreetly by this mycologist whose got MDMA, chocolate bars, Isd, dmt, Mushrooms, cannabis etc

[@Raye-ml1hx]

On Instagram or telegram......

[@Raye-ml1hx]

@Pham_smart11

[@Meadsy5]

Awesome videos and channel! I’ve recently grown a batch of mushrooms that I’ll be using to help my symptoms with concussions; and your videos were a huge help! I was wondering after the ethanol extraction, could I use my Turbo Source Pro (closed loop, low temp extraction under vacuum from Extract Craft) to remove the ethanol so I can have a smaller end product to make into gummies? I’m sure I’d have to cook off the water extraction until it was a smaller volume as well to not dilute the gummy mixture. Thanks for any insight!

[@Fungiacademy]

Yea people normally use a rotovap for this but you could try for sure!

[@toddronald8753]

Is this method any better than a dual extract using mason jars and the shake over time method ?

[@Fungiacademy]

Myself, and lots of people think so yea! I also have a friend who got his tinctures lab tested and saw like 30% higher active constituents present. + It's way faster

[@SoloSp33d]

Hey! If I want to use ultrasonic cleaner for mushroom extraction, should I run the cleaner with alcohol or water ? And if I want double extraction. Can I dilute alcohol with water, to keep alcohol about 30% vol and use ultrasonic cleaner?

[@Fungiacademy]

Honestly I dont use the ultrasonic cleaner anymore because it wasn't really effective

[@predicateable]

Thanks for the video, clean and concise! Btw, why do the 2nd extraction with the water at all? Is there anything left in the source material after 6 hrs of alcohol extraction, that another 4-6 hrs of water extraction can add to the final product? What would happen if you eliminated water extraction step completely, and just dilute alcohol extract with clean water to the desired 30% of alcohol?

[@predicateable]

Another question is how do you determine the recommended intake dosage for tincture? How do you personally determine how much tincture you should take every day? Do you have a lab to test the concentration of active ingredients?

[@Fungiacademy]

Quite a bit actually youll see the impact the taste and effect the tincture has when you combine the too. Diluting is never a good option in our opinion

[@Fungiacademy]

Lots of tincture is good for us here! We are in rural Guatemala so no lab for us just yet...

[@predicateable]

@@Fungiacademyyeah, I've googled it after I've asked the question, and it seems that alcohol and water both extract different elements from the source, hence, double extraction.

[@predicateable]

@@Fungiacademythe question about the dosage is quite important when it comes to the question "do I take enough?" (for example, when taking magnesium, or D3, or whatever, taking too little does not make any effect for the health). Or with "spiritual" mushrooms, it might be the opposite, i.e. "will this be too much?". Is there an idea how to measure the tincture dosage for intake? But with medicinal mushrooms the effect is not apparent (unlike the spiritual ones), so... is the dosage enough to make a difference in the body/mind? And also if you sell your tinctures, the customers would expect a guidance on the dosage. Probably, the approach might be like the following. If 100ml was extracted from 100g of mushrooms. And let's assume 100% yield/efficiency, then 1ml of tincture is equivalent to eating 1g of mushrooms. Then, it seems that 20 drops from a standard dropper is 1ml (the internet says so). Therefore, 20 drops of tincture = 1 gram of dried mushrooms. Then, the big question is yield efficiency. Probably, science papers can provide some guestimate on an average efficiency for the soxhlet method, but I suspect it highly depends on the matter being extracted, thus, will vary even between different mushroom types/species. But at least they say it's more efficient then the "soaking" method. Do you have any kind of such estimates, or method, or whatever?

[@selurxelpirt]

Have you tried methanol??

[@Fungiacademy]

Why would I? I'm trying to put this stuff in my body here

[@myfalconry76]

Natural extraction using honey worked fantastic and it’s sweet

[@michaelcarey9359]

I don't know what you'd extract from cordyceps using honey, but this guy is making a tincture, like a medicinal product, not some kind of muck that you couldn't give away, let alone sell.

[@myfalconry76]

@@michaelcarey9359 then you know very little because the enzymes in the honey break down the cellulose in the mushrooms releasing all its properties into the honey being trapped by the honeys sugars and fats the honey is then filtered and maintains the properties of the mushrooms. It can then be added to anything you want from teas to juices even mixed with cake mixes, and brownies cookies, chocolate. Indigenous people around the world have been using this method of extraction for thousands of years. Even used with other medicinal herbs to create antiseptics and topical ointments. Also used and mixed with other medicinal oils to make tinctures many of which are sold at certain traditional health food stores

[@myfalconry76]

@@michaelcarey9359also mushrooms are heat and light sensitive on a molecular level the heating of the mushrooms kills and breaks down a lot of the helpful properties. Effectively destroying its medicinal properties. 100% fact just like cooking vegetables removes the nutrients they contain at a rate of 60-70%

[@WestbrookOwnsU]

@@michaelcarey9359 He's talking about blue honey made out of cubes. Doesn't taste like muck, probably sells well.

[@Chris-no2li]

I learned that mushrooms like Reishi etc. needs pressure to break up chitin?

[@Fungiacademy]

Mmm just hot water is enough

[@1akmason]

I don’t know if anyone else feels this way, but I say you look so much better with the long hair. It just seems like you if that makes any sense.

[@skinnyd_tk6795]

Hi, Chemist here. Just a little nit pick, the volume scale on Erlenmeyerflasks are more of a suggestion, they aren't accurate at all. If you really want/need to be precise, you should opt for a volumetric flask, measuring cylinders, or pipettes. Cheers, and take care

[@Fungiacademy]

They've been lying to us!?

[@skinnyd_tk6795]

@@Fungiacademy not really lol, as i said it's just a suggestion. if you need to add distilled water for titrations for example, you don't need to measure it accurtely so you can just use those instead. but for this instance, it should be fine dw too much about it 🙏

[@Fungiacademy]

@@skinnyd_tk6795 Haha i was just kidding, thanks for the recommendations tho!

[@taelend5301]

does the heat affect any of the mushrooms medicinal properties when extracting?

[@Fungiacademy]

I think it's debated if the heat affects the property levels, I've had friends with commercial businesses test their products after Soxhlet extracting and they seem to be really potent, even after exposed to prolonged heat

[@xjunkxyrdxdog89]

Check the decomposition temp of your target compound, and compare it to boiling point of your solvent. If the boiling point of the solution is below the decomposition temperature you won't destroy the target compound.

[@davidb7723]

Thank you so much for this video! There's a few duplicate links in the description (erlinmyer flask links to cheese cloth). Any chance that can be updated?

[@Fungiacademy]

Thanks for pointing it out! Ill look into it tomorrow :)

[@Enochulate88]

Does this work with phycadelics?

[@Enochulate88]

@Crystal-bx7qv yeah cause it's so hard to make what a joke

[@Tonyneg]

Yes, it does, you just should use methanol instead of ethanol. Using methanol, you absolutely need a vac oven or even better a rotovap to make sure all the methanol is gone. Also, yes methanol can harm humans, but it works way better for psilocybin containing mushrooms so just make sure it’s fully removed and it can’t hurt anything but your ego.

[@Enochulate88]

@@Tonyneg is there like a tek name?

[@Tonyneg]

@@Enochulate88 no, it’s just extraction with methanol. It’s about as simple as it gets.

[@Enochulate88]

@@Tonyneg yeah but I don't want to leave any of it behind xD

[@neilgc4943]

Great video. Very informative. Thank you for sharing.

[@Fungiacademy]

Thanks for watching!

[@what_Love_Drew_forth]

Some people soak mushroom matter in the 95% alcohol for 2 weeks then just strain it. Is this method in the video more effective?

[@mateomerchan164]

Hi Jasper! isn´t destilation necessary?

[@Fungiacademy]

Not at all but cool if you got the tool!

[@marvin216]

How did you get a license to sell the extracts?

[@Fungiacademy]

Depends on where you live! We just make the tinctures to barter with friends and have our own medicine

[@Raye-ml1hx]

I got my stuffs delivered discreetly by this mycologist whose got MDMA, chocolate bars, Isd, dmt, Mushrooms, cannabis etc

[@Raye-ml1hx]

On Instagram or telegram......

[@Raye-ml1hx]

@Pham_smart11

[@hyperportal8899]

We was using soxhlet but since upgraded to sonication.

[@Fungiacademy]

The natural evolution!

[@timothylongmore7325]

I amke my own dual extracts on a small scale and this looks like it would work great for me. If you guys did chaga would you do the hot water extract first? To break down the chitin walls and make the Alcohol extraction go quicker. Trying to speed up the process. Also do you use your spice grinder for dry reishi.?

[@Fungiacademy]

Generally I do alcohol first with all mushrooms, but that's old practice from the oldschool way of doing it. Right now I do it because doing the water extraction first will dilute the alcohol since you basically soak the mushrooms in water

[@timothylongmore7325]

@@Fungiacademy Makes sense. I've been doing a crockpot size batch of HWE of chaga, then redrying the chunks, in into the alcohol soak. I like your rig and would fit my op for every thing except the chaga. Thanks for the reply.

[@tomdeans]

Ethanol is not for sale to the public in many countries. What alternative solvents do you suggest ?

[@Fungiacademy]

someone suggested ether, but just buying Vodka or any off the shelf liquor with minimal or no sugars could work.

[@MoreFootWork]

Hi. This soxhlet is about how much ml?

[@Fungiacademy]

The flask is 1000ml and the soxhlet itself 500ml

[@MoreFootWork]

@@Fungiacademy thank you :)

[@Raye-ml1hx]

I got my stuffs delivered discreetly by this mycologist whose got MDMA, chocolate bars, Isd, dmt, Mushrooms, cannabis etc

[@Raye-ml1hx]

On Instagram or telegram......

[@Raye-ml1hx]

@Pham_smart11

[@crazedzombie8676]

How long will the extraction last?

[@Fungiacademy]

If you store it properly for a loooong time!

[@The_Mad_Arab]

Hello thank you for this video can I ask what micron your resin bags are? or what you would suggest?

[@Fungiacademy]

The link in the description sents you to the one we use!

[@The_Mad_Arab]

@@Fungiacademy I definitely picked that up but there's still several different sizes to choose from wasn't sure which one to start with

[@starbear1322]

Thanks alot for sharing. My question is, can i just walk into a glassware supplier and buy apparatus?

[@Fungiacademy]

We put the link to the soxhlet in the description!

[@3319david]

Which method is better, ultrasonic or soxhlet?

[@Fungiacademy]

if you got that money Ultrasonic for sureee

[@chnebleluzern]

what about using a 30% ethanol 70% water solution in the soxhlet from the start and heat it up to 100°C ?

[@Fungiacademy]

mmmyea I tried that for simplicity but i didn't like the results, have never been able to test the actual results tho

[@rickwilliams967]

Could you do some blue ones?

[@Fungiacademy]

I know some people that are doing it!

[@intothefoxhole7985]

What kind of greese do you put on the joints? Please be specifics. Maybe food grade silicone?

[@Fungiacademy]

I don't grease my joints and have had no issues🙃

[@intothefoxhole7985]

​@Fungiacademy thank you for the reply. I didnt think it was safe to put 95% in the boiling flask. How long have you been running it like this and is there anything your doing to stay safer? Ive always stayed below 49% for saftey.

[@velvia7880]

What about the water soluble compounds?

[@Fungiacademy]

we do the water extraction after the alcohol extraction :)

[@vazap8662]

I have a question, hopefully this will be seen.. I'm about to buy a soxhlet, but I only need to make small batches, like 30-50ml, which will translate in 15-25ml runs for dual extraction. Is a 1000ml system still usable or totally inappropriate? Is it ok to run batches much smaller than the system's dedicated volume, or would a lot of wastage ensue? Or should I aim for a 150ml system, even though these all seem to come from unknown brands I'm not sure are entirely trustworthy?

[@Fungiacademy]

I'd just make 1l batches and store the tinctures for later use!

[@vazap8662]

@@Fungiacademy​​⁠Thanks for replying! It’s just that 1l would imply a lot of mark… too much for the species I’m doing. I opted for a 250ml flask and thought I can always reduce right? As in, turn off cold water and let the solvent evaporate.. is that done?

[@michelebettio9328]

Hi Jasper! Thank you for this amazing video, if I can ask what is the ratio of potency between dried mushroom and tincture? (e.i. eating 1g dried is the same as drinking 1g tincture) With soxhlet is obviously much faster but regarding potency is the result equal to the mason jars method?

[@Fungiacademy]

Hey Michele, this is a complicated topic and I wouldnt be able to give you a solid answer without having acces to a lab that can test these things for me. In theory, the tincture should be more potent, just because you're eliminating chitin and making all the myconturients more bioavailable. Lastly the colors of our Soxhlet tinctures are on another level compared to the mason jar method, they're fuller and darker and although i can't say for certain without testing they look and feel way stronger then how we used to do it.

[@endoxosskopos]

How many milliliters is the extractor?

[@Fungiacademy]

Flask is one litre, soxhlet is 500ml i believe

[@SporeWhore661]

Whered you get your soxlet setup?

[@Fungiacademy]

Link is in the description! We just got it on Amazon

[@marknichols7861]

Can a product like “Everclear” be used in this process? It’s a 190 proof grain alcohol.

[@Fungiacademy]

I think some people use everclear, I googled it and it seems like rectified spirit alcohol should be safe for consumption

[@bencevarga6304]

Heyy guys! Do you know of any research how an extract holds its potency compared to dried whole fruits?

[@Fungiacademy]

Anaerobic environments don't oxidize so it should be more stable

[@SirFancyPantsMcee]

and you dont have to worry about the heat damaging the extract?

[@martinahozjan4966]

777! Yees! Love your set up.

[@nicolasrowe03]

Does it work with psilocin

[@anchopanchorancho]

If you use the right solvent, then yes.

[@timothylarsen9521]

Will extracting this way for ‘sacred mushrooms’, using heat, (boiling alcohol) degrade the medicine in the mushrooms?

[@michaelcarey9359]

Knowing exactly what you are extracting is as important as choosing the solvent, and the temps you'll be using to reflux.

[@KieranChallis]

What micron are the rosin sachels?

[@Fungiacademy]

Honestly I forget🙃

[@niklashultkrantz7766]

I was thinking using a espresso machine replace the coffee with mushroom powder and replace the water with alcohol.

[@Fungiacademy]

You could try this, let us know how it goes :)

[@getprobed838]

i wonder if i can put san pedro cactus skins in it and extract the medicine. anyone know if this would work?

[@Fungiacademy]

Yea probably

[@alllove1754]

Now this is so cool!!❤

[@tzachikirshberg9799]

How do you fill 50 grams of Reishi in the bag ? It’s to stuffed. The water doesn’t flow through the bag nicely

[@Fungiacademy]

itll flow around it, that's why the thimble!

[@cestlaviehappner1872]

So it doesn't matter the size of a satchel you get like for a 250

[@Yassous83]

Absolutely love the important work you do, your generous content (especially the awesome free courses that are the best gateway drugs out there - I will absolutely be signing up for your courses 🙌) and the fun vibes you bring to it all 🙏

[@Fungiacademy]

Thanks for the kind words! Fun vibes are crucial!

[@subsonicelf9271]

Wonderful video.

[@Fungiacademy]

Thank you very much!

[@KieranChallis]

Just a word of advice! You shouldn't do the alchohol extraction first. Beta-glucans are insoluble in alchohol so the left over residue in the mushrooms will contaminate the water extraction and could cause many of the beta glucans to solidify and be filtered out. Beta-glucans are the primary active compounds in many medicinal mushrooms so preserving them during every step of the process is important.

[@nileclac4703]

Hello, so what should you do regarding beta glucans?

[@etownKeystoned]

​@nileclac4703 (you probably won't hear a response because it's KZbin and just anyone can comment) you start with alcohol because it breaks down and prepares the mushroom (cell walls for example) for the subsequent water extraction. The alcohol soluble compounds are diminished & their structure changed by heat, so you pull them, triterpenes primarily, first, before the hot water extraction. So: the alcohol breaks down & prepares compounds to be extracted in the subsequent water extraction in a manner water first would not achieve- this is an efficiency reason. The compounds that are pulled first being heat-sensitive is a technical reason. That doesn't make the OP incorrect, it just means the benefits of alcohol first are much greater than the risk of... contaminating the water??.... or whatever it was they stated in their thoughtful but potentially misguided words of advice. Now you may be after the beta-glucans and everything else is secondary, sure, in thst instance it makes sense to hit it w/ water first

[@nileclac4703]

Hello, thank you very much!

[@glenmarshall8168]

I thought you were supposed to do water extract first then alcohol extract? Thanks for the video BTW 👌

[@Fungiacademy]

Honestly ideas diiffer on this, I always learned to do alcohol first and lots of people I know do the same but what works for you works for you!

[@andrerodon3921]

How many gallons of water are going down the drain for one complete extraction? This cooling method seems wasteful and costly if I'm understanding correctly and cold water is constantly running from your sink, through your system, and down the drain for many hours on end...

[@Fungiacademy]

Our grey water goes into our garden and feeds our plants during the dry season so nothing is wasted