How to Spot Contamination in your Agar, Grains & Liquid Culture

Рет қаралды 18,168

Күн бұрын

Пікірлер: 41

@heidik.9005 6 ай бұрын

When my husband started growing mushrooms he lost a lot of them to trichoderma. I couldn't understand how things got so bad without him noticing and then we realized it's because he's red-green colorblind and could not tell it was green until it had completely taken over. Now I go through and check things for him 😅

@Algumnomequalquer 5 ай бұрын

but how do you fight tricho ? normally I throw it away😅

@Algumnomequalquer 5 ай бұрын

@heidik.9005

@dl2415 4 ай бұрын

@@Algumnomequalquer prevention. though there are polymers you can get to coat it and stop the spread

@TheQueenOfDreams 4 ай бұрын

I don’t need to see it to know there’s trich, I can smell it a mile away. 🤢

@SubFlow22 9 ай бұрын

Thank you for the awesome content! I always struggle to ID contam in my LCs.

@Fungiacademy 9 ай бұрын

Thanks for watching! Altho sometimes you can see contam in LC I always test mine before using it

@janhus4987 9 ай бұрын

Bravo,very nice video,loads of knowlange in it. TY

@Fungiacademy 9 ай бұрын

Thanks for the kind words Janhus!

@MacCelium 9 ай бұрын

Great vid...thanks for all you guys contribute. Would love a vid just looking at a bunch of agar plates and describing what you are seeing. This would include contam, but also various kinds of growth patterns. Even after looking at hundreds of plates, when I see mycelium taking on multiple phenotypes, I'm always curious/nervous about what is going on and if I might be missing something. We are at +7000 feet so things are pretty clean, but getting a good sense of what we are looking at is invaluable. Mush Appreciation!

@Fungiacademy 9 ай бұрын

That's where the practice comes in! It'll become like a second nature one day. Thanks for the sweet words!

@DNBAnnex 5 ай бұрын

Great info!

@Fungiacademy 5 ай бұрын

Glad it was helpful!

@cocoakusubuu7100 9 ай бұрын

when i first started growing mushrooms, the place where i was at had a problem with that orange bread mold stuff. it's hard to catch because it grows like mycelium, white n fluffy, until it matures and turns into an orange dusty spore mess that gets everywhere. has a putrid dead-fruit smell. When i first started growing my subs were way too moist, for sure. lol i adjusted quickly and ended up growing over a dozen species. I love mushroom so mush. :*)

@Fungiacademy 9 ай бұрын

Neurospora crassa is the worst.....

@etownKeystoned 8 ай бұрын

as someone who has grown (and is growing) just about every type of mold feasible, i have to say, thats some pretty gnarly mold in those dishes, especially on that gourmet TAT strain. Lordamercy, that bad boy is a biohazard.

@Fungiacademy 8 ай бұрын

Welcome to the jungles of Guatemala haha

@lazyladd5354 6 ай бұрын

I agree it’s crazy he even suggested doing a transfer to an audience of mainly new growers.

@Mi_Mono 9 ай бұрын

Great tips. Keep up the amazing work!

@Fungiacademy 9 ай бұрын

Thank you Mr Mono🐵

@1979pmiller 5 ай бұрын

Contams can also be embedded in the mycelium.

@Fungiacademy 5 ай бұрын

Or endospores, it's all an intricate web

@ap8riot931 3 күн бұрын

I got tired of transferring good mycelium just to find out it was trich contaminated. I got a 100 power hand held microscope and thought "I wonder if I can see the trich spores?" Yes you can. They are little black globes hiding in the mycelial nets. I have saved myself a lot of wasted setups by checking first.

@monk5398 7 ай бұрын

Thank you for this video. I have a question for you concerning contamination. I spawned a green jar with PE. The inoculation was coming along very well, with most of the spawn jar colonized, but recently I noticed colors in it that I have not seen in other jars and I'm wondering what they mean and if it is contamination? The green was not normal green of trichoderma but was a bright neon green-blue. Also, there was a bright neon pink in it as well. I was happy to attribute the blue-green as bruising, but I am bewildered by the pink...

@Fungiacademy 7 ай бұрын

Pink is generally other fungi meaning your spawn is bunk unfortunately

@SolarpunkSteven 8 ай бұрын

How do I come out there with you guys lol

@shrabinlorg 8 ай бұрын

This question isn't so related to this video, but I wanted to ask this in the more recent content hoping for an answer. Can anyone tell me of where, in the US, I can send my medicinal mushroom extracts to have them tested for desirable bioactive constituents?

@Fungiacademy 8 ай бұрын

Mmm it's difficult testing needs to improve

@llihpmeHnevetS 9 ай бұрын

What smells minty as far as contamination?

@Fungiacademy 9 ай бұрын

Mint? Jokes aside I've never had this

@llihpmeHnevetS 9 ай бұрын

I was confused as well. Notice the funny smell started looking closer and found a nearly white light grey in a cake that was just put into fruiting. I cut the spot (size of a half dollar) out and just as a test I sprayed it with alcohol it immediately turned blueish green. The cake went on to pin and the smell came back. I couldn’t find it this time and all pins aborted. I disposed of that cake.

@ap8riot931 3 күн бұрын

Gardnerella

@biswakarmababooraam3823 8 ай бұрын

How can I solve with chemical treatment

@HappyMayCope 7 ай бұрын

So I'm a new grower..My first grow actually..I inoculated 2 grow bags..I noticed about 4 weeks in green spots of trichoderma in the mycelium colony. So I was thinking.. And it occurred to me..😊 CDS.. It's a water purifier.. It's a water soluble gas.. And kills fungi bacteria viruses on contact.. But when it's not contained, it does what gas does and evaporates, leaving sterilized water.. So I used a watered down solution very low ppm.. I took a funnel and put the bottom of the funnel right on top of the green spots..Dropped litterly 1 drop at a time..And it killed the trichoderma immediately.. And in seconds evaporated leaving the mycelium un harmed..7 days later still no signs of the trichoderma.. So this might be them chemical solution you were asking about..

@lazyladd5354 6 ай бұрын

Why would you even suggest doing a transfer from that TAT disc Contaminated with black mold that is wild. Everything you said was pretty relevant until I heard that then that’s all I could think of for the rest of the video that is a terrible idea. Not only are you running the risk of letting that contamination spread like wildfire into your work area and other grows but that stuff is really horrible for you and can even kill you. There’s some things that you can save and there’s other things that you just discard and that’s one of them.

@Fungiacademy 5 ай бұрын

Honestly I toss any contamination because I have back ups of everything. But I've also been in scenarios where I only had one plate, contaminated with black mold and no back ups order spores and made clean transfers from them so yea I think it's valid information to share