HPLC Method Development 20 Nov 2022: Q&A Session 1

[@esakkiammalsudhaesakkimuth9202]

Thank you for your great work!! Q & A session is very useful and impressive!!

[@nikitaborade3806]

Sir please upload yesterday's video on gc method development and troubleshoot

[@HarikrishnaTirumala9]

Thank you so much sir..🙏

[@trendbizz2743]

Good evening sir I have a question that im having a combination of two drug product have same nano meter now im getting one unknown impurity peak in middle of two drug peaks , im doing dilute standard method so how i will calculate that unknown impurity with respect to which drug peak and that impurity is not in degeneration data , not in Drug +Excipient study , stability data not in placebo blank ? So how i will get to know that unknown peak belongs to which drug

[@sunandavalvi2298]

sir how to make a dilutions of herbal extract for hplc

[@abhishekjoshi9650]

how to decide gradient ration in related Substace method?

[@samadhan.p1298]

Sir, please release video on quality control instruments having involved UV, IR, KF, PH, SOR, Autotitrator

[@abhishekjoshi9650]

How to choose or decide or change gratient ratio?

[@ajeetsingh12499]

According to the resolution , when you get bad resolution then change your gradient ...

[@pankajnagwani3880]

Sir i also have a question that before doing analysis in HPLC instrument what are the conditions or fact about which we should care to select the mp

[@abhishekjoshi9650]

Great

[@pankajnagwani3880]

Very nice session sir although I was not participated but it is very informative session

[@omarfaried1111]

Firstly, I would like to express my gratitude for your great efforts. I have a few questions, please: Why is ethanol not used instead of methanol? What is the benefit of a pore size of 120 angstroms or more or less in relation to the analyte I need to separate? How can I determine the polarity of my analyte before starting chromatographic separation to have an idea of the order of separation? What are the acceptance criteria for receiving a column with respect to the theoretical plate reference limit? Why does absorbance vary with different mobile phases or columns for the same components at the same wavelength? In the column regeneration process for normal phase chromatography, I use water in one step. Conversely, in reversed phase chromatography, heptane is sometimes used. Is this harmful to the silica? Why might different mobile phases alter the separation order of analytes, even though the principle of separation is based solely on the polarity of the analytes and the stationary phase?

[@EARTH_THAKUR]

I want to develop sodium hyaluronate method on hplc please guide

[@krish_krish354]

You try elsd detector with hplc will work