Next Generation Sequencing 2: Illumina NGS Sample Preparation - Eric Chow (UCSF)

[@DixOutForHarambe]

This is one of the few videos which accurately describes all needed details for NGS-beginners. Thank you so much dude! It is so much clearer for me now.

[@fahadmehmood1685]

Great to see such a detailed and understandable video related all about NGS.

[@Lenz979]

It is always a pleasure to listen to you Prof. Chow. Thank you so much for taking the time and doing these videos for us!

[@joye.4281]

🤓Thank you soo much for this video! What I am doing now makes all sense. This is my first time working for a biotech company that also uses illumina sequencers. For someone who only worked in hospital core lab it was hard for me to understand biotech processes. This video helped me a lot and not just doing the steps in the lab without understanding. 💯 🙌

[@nicolaeionescu-kosa132]

It's a pleasure to listen to Prof. Chow

[@angie800710]

Thank you for providing a well-explained tutorial video for the NGS beginner!

[@studybooks3395]

You are so smoking hooot!!!

[@metipsm]

Excellent presentation. Thank you.

[@honeybunbadger]

You're the best, Prof. Chow! These videos are fantastic.

[@zulucharlie5244]

Really great information - thank you.

[@nehagoveas1]

Thank you! This was so wonderfully explained. Truly one of the best video out there!

[@NRoy877]

This is so simple and accurate, thank you for your video

[@peterlauridsen8403]

This guy is so precise and thorough

[@cientistarj]

I've been learning so much with you, prof. Chow. Thank you so much!

[@kmacdowe]

Complicated process explained simply. Thank you for the good work.

[@sweeties6383]

it`s very informative . Nowadays I`m learning about sequencing . Thank you so much Professor.

[@mly2883]

Thank you Eric. Much needed video.

[@llsa2009]

Very nicely explained video! Could you make video to explain different Lib Prep kits from various manufacturers with pros and cons?

[@rayzhang928]

these videos r so good

[@ccdj35]

You explained it perfectly. Thank you.

[@DrApichatPhotiA]

It's very informative>>> You can simplify it. Thanks

[@Tapj]

Thank you Eric!

[@chepai1827]

Nice talk!

[@kw-kt9xr]

If I had these videos 2 years ago I'd be getting a first for my first year instead of a low 2.1

[@atrakchi1]

Well explained. Thanks!

[@shih-yihsiung239]

Thanks for the video.

[@mantidream8179]

Excellent explanation

[@isabelluo8752]

you are a true life saver!

[@areejkhatib9095]

Amazing, thank you

[@dorisonuzulu9388]

Thank you SO MUCH!

[@Andrea-sh9sn]

Thanks a lot. What would happen if instead of putting 5% PhiX in the cartridge, I mistakenly put more amount (60%). And I put the correct amount from the library?

[@eudeciogabriel8571]

Interesting how you can do this with something so small.

[@desanonima]

Very nice, thanks!

[@1856chi]

Can you please do a video on Bionano?

[@emavalenzuela8085]

Hi! Thank you for this video. I have a question, how meny samples can I sequencer in a single flow cell if I'm doing exome analysis. Thank you

[@merlindavid16]

I wish Prof. Chow taught me in college

[@devinyoung5735]

can you define what a "library" is in this context? Is it a term that comprises all of the fragmented genomic pieces?

[@bashiradilme]

your are awsome guys ...thank you

[@王昌令]

As every target sequence has unique primers and PCR temperature.How to design PCR primers and protocol for amplification-based enrichment library preparation?

[@charlesw1973]

It's usually done by inorganic synthesis chemically and by other companies like IDT.

[@denizkarayagmurlu7162]

Can anyone please tell me, why 5' ends of fragments should be phosphorylated ?

[@Philosophyof]

could someone tell me why are we using lower and upper marker?

[@muhammadashrafhadirosman3845]

Good explanation :D

[@sam2theammyk9]

13:48 I am so confused. How can you flip the orientation of DNA? I thought it was sequenced 5' to 3'? Are you sure the complement isn't being read? I don't see how the template can be read twice by flipping it because the orientation would then be 3' to 5'. That does not make sense to me. I'm so confused :(

[@charlesw1973]

It's actually still adding from 5' to 3'. It's just with the different indexes now The result strings looked flipped. Look up the illumina NGS video and you'll see the addition happens after the bridging step and is going from 5'3.

[@sarmadyousif2179]

Are all the DNA sequencing technologies need library preparation ? Yes or no ?

[@elleffeff]

so far, yes.

[@LudivinaPretti-m2h]

Conner Motorway

[@anhtruong2636]

the video image is too poor, you need to fix it more