Microscopy: Phase, Polarization, and DIC (Stephen Ross)

[@AMadScientist]

Hello. Thanks for explaining. What I'm trying to find out is how a DIC scope would view live protists? I see videos on KZbin with these great clips using a DIC but I was wonder if it is just "drop the water on the slide and go" or is there tons of prep time to get the "good" shots. Thanks

[@HaloGrndr]

Any chance you could provide some part numbers for the polarizer/analyzer components? I can't find anything!

[@source1289]

was the lower prism left in for phase contrast ?

[@ClownWhisper]

I just got an older TSM inverted microscope and I was wondering is there a camera mount or a camera made that specifically fits the neck of this microscope? I have seen what I thought was a TSM equipped with a completely different neck with a camera going straight up and the regular binocular assembly in its usual position. I have no idea what I should look for can you please help me?

[@allthegrassisgreen]

Does having both PLM & PCM in the same microscope comprimise either of their qualities/accuracy? Pros are obvious, cons?

[@Satchmoeddie]

Where can I rent one of these and all the goodies for 2 hours? Amazing scope! Nikon, Canon, Leica, all make nice stuff. I need to do some color phase shift work on 4 samples. That is a great unit! How many human kidneys does it cost?

[@abskateboard]

not to mention zeiss lol

[@JakeSchonhoft]

Can't forget Olympus. Been making microscopes since 1919.

[@donsimons9810]

color phase shift work? not to dork up the lingo, but what do you mean by that?

[@Dhelira]

Very enlightening. Thank you

[@Thesignalpath]

Thank you.

[@johndubose6589]

Thankyou!

[@MrGunzoller]

Why use expensive DIC method while you can just use oblique lighting and to see absolutely the same 3D effect? You don't even need a specific instruments, just ordinary biological light microscope. All you need is to play with condenser diaphragm (about 50% closed), opaque screen in front that diaphragm and light volume.

[@donsimons9810]

do you have a reference for this technique used in microscopy?

[@MrGunzoller]

@@donsimons9810 kzbin.info/www/bejne/pZS3dolsf7SgjMU

[@FieryCoal]

Although Oblique is useful for its cost, it gives off a lower image quality than DIC.

[@VicenteFlorencio]

astrolab

[@abedhossini5793]

boring not sobject yor act