Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs

Immunohistochemistry (IHC) is a technique used to visualize specific proteins or antigens in tissue sections. This method involves using primary antibodies that recognize the target protein, followed by secondary antibodies that bind to the primary antibodies and are tagged with a detection reagent, such as an enzyme or fluorescent dye. The detection reagent produces a signal that can be visualized under a microscope to identify the location and distribution of the target protein in the tissue section. One common strategy for enhancing the sensitivity of IHC is to use an amplification system like the Vectastain ABC Kit.
The ABC (avidin-biotin complex) method involves using biotinylated secondary antibodies to bind to the primary antibodies, followed by incubation with an avidin-biotin-peroxidase complex. The peroxidase catalyzes a reaction that produces a colored or fluorescent signal at the site of the target protein, allowing for detection with high sensitivity and specificity. The Vectastain ABC Kit from Vector Labs is one popular option for performing IHC on paraffin-embedded tissue sections.
In this process, we cover the dewaxing, hydration using an alcohol gradient, heat-induced antigen retrieval, blockage of endogenous peroxidase activity, and non-specific binding sites used in the production of paraffin-embedded sections. Then, certain sections are stained with CD8-specific antibodies, and other sections are stained for tyrosine hydroxylase.

[@adamischaos6121]

What is the sodium citrate solution diluted with?

[@Educationalcourses]

If you will prepare 1L stock solution so, you will take 2.94 g of Tri-sodium citrate (dihydrate) then add 1L of Distilled water Mix to dissolve. then Adjust pH to 6.5 by adding 1N HCl.

[@adamischaos6121]

@@Educationalcoursesthank you!