Inoculating Agar and Grain in a SAB (Still Air Box)

[@MikeCanFly]

Ya...I used alcohol, and lit my lighting inside, and my box was air sealed with gloves.....it exploded. It was an awesome mad scientist experience. Lessons learned.

[@calebtheshamen7033]

I'm still gonna use alcohol

[@Mushables]

You still have hands?

[@stitchywitchbritt2125]

Lesson learned the hard way myself. 2nd degree burns on my neck and my hair is scorched to the scalp in some places. Bleach works fine, and alcohol on towel inside if any. Wipe items before putting in the box.

[@lovelost28]

lolll glad I'm not the only one, I just flashed my box like 2 or 3 weeks ago, my right hand is messed up but it healed now. Well, at least the box is extra sterilized from that flash. I actually thinking of a safer way to flash my box like that again because in my theory the flash will push any particles out and the heat will destroy any microbes inside.

[@briannansnow1740]

I did same lol-on a very small scale lol

[@toddwmac]

Really appreciate you going through these techniques w/out using your FH. I'm surprised how many YT channels go though basic techniques (like inoculating a bag) sitting in front of a flow hood. Not to those guys...viewers that have flow hoods don't need to learn how to inoculate grain bags. :) Appreciate your content.

[@rudyruiz7962]

I always refrain from working directly in the lid as most of the dirty particles like to chill there. I use a small shelf divider to work an inch or two above the lid

[@midnightmushroomco.2091]

That's actually a great point!

[@bigbrotheriswatching2680]

I'm going to try using a damp towel soaked in water with ~5% bleach to seal the floor of my SAB and just leave the lid off completely. I saw MycTyson using this method.

[@mintislad3900]

I lay aluminium foil down and wipe it down I don’t trust the lid

[@christian9506]

I usually use one of those grated cooking trays. Super cheap and available everywhere

[@natedavis7750]

@@bigbrotheriswatching2680 how did it work

[@jolIyjoeker]

For SAB holes if you take a 28oz food can and put it upside down on stove for a minute, and then in a well ventilated area using a glove use that to melt your holes. Clean melted plastic off of the can in between doing holes, works perfect!

[@chicofromph33nix64]

Freaking great tip... i was just thinking of what would work best.. thx for sharing that...

[@bclark3614]

This is an awesome little hack! Thanks for sharing it

[@christophermccurry8039]

that is an absolutely brilliant tip for any newcomers that may not have a drill or hole saw. Wish I could like it twice.

[@SillyWabbit120]

Hey does it Go through pretty easy or have to reheat a Couple times to make the hole? Also what size Tub do you recommend?

[@villain-de-cat]

I just got my sab and wiped it all down alcohol 70 same for the out side of the all in one grow bag put it an my spore syringe in I had a new in the sealed package needle so I didn't burn it hope that was not a mistake and it set it up all in my closet so it can stay in the dark and at a 70 degrees I picked a strain called butters but now I don't have a clue what to do next

[@DrGaryGreen]

Bleach instead of alcohol. No boom. Great idea. Thank you.

[@urmommaahugehoe]

Just make sure to not mix the two, bleach + alcohol = chloroform :)

[@HavelockVetinarii]

@@urmommaahugehoe Whatever man I can handle my inhalants

[@emilys2849]

@@HavelockVetinarii -how 0-0

[@HavelockVetinarii]

@@emilys2849 kzbin.info/www/bejne/q3yVZGalirKIqc0

[@user-_-404]

@@HavelockVetinarii lol

[@shawnwillis7561]

Just ordered a couple grain bags with free agar plates. I've had so much trouble trying to make my own agar. I am soooooo glad I found an agar supply that is not only inexpensive, but so close to where I live. Can't wait to get order and get it growing

[@midnightmushroomco.2091]

Thanks!!

[@c.odubhlaoich2948]

Shouldn't be hard at all to make clean agar. Maybe it's where you're storing it that is making it dirty. Look at no-pour teks if you still have trouble, but put your agar in new (already sterile) ziplocks or even just spray the insides of already opened ones with alcohol or Lysol before putting in the plates.

[@c.odubhlaoich2948]

It's been a month so surely you've used yours already. But I should add..making clean agar is easy, especially with no-pours. Ordering clean agar won't help if technique is bad, and storage is bad.

[@shawnwillis7561]

@@c.odubhlaoich2948 it's easier to keep grain from getting contaminated then agar. I think when making agar you almost have to have a flow hood, which I don't have yet. For now I have a s.a.b. Hopefully soon I'll be able to have the space for it.

[@c.odubhlaoich2948]

@@shawnwillis7561 Are you talking about for pouring or doing transfers? You only need an SAB for transfers, but like I mentioned, you can do no-pour agar which of course requires no SAB to make because it's already sterile inside the containers.

[@TheSporceror]

Maybe I haven't looked hard enough but I haven't heard anyone else mention not using an open flame in or near a SAB if you are using alcohol. It seems obvious now that you mention it but honestly don't know if I would have even thought about that. Thank you for the safety tip and for all the other awesome information you shared in this video!!!

[@midnightmushroomco.2091]

It’ll definite cause some burnt eyebrows lol

[@TheJessC]

90 second mycology mentioned it i believe.

[@selvakumarrajendran9872]

That’s one really great safety tip I’ve heard no other videos mention . Thanks man for this video 🤘🏾

[@michaelg8642]

using a flame in the still air box also creates air flow because of the heat of the flame (convection)… so partly defeats the purpose of the SAB

[@Mind69420]

We probably need some boom videos to go viral before this info will be widespread common sense. I've only heard horror stories on the shroomery forums.

[@dustinmoore1559]

Hey your my long lost dad, well I'm following in your footsteps with mixology. My birthday is in June ive really needed a flow hood like yours.

[@eljar3976]

you can build one yourself for around $180 son. Start saving your allowance, pick up a paper route this summer, and when you're ready some nice strangers on the internet will offer you some assistance

[@Six_Foot_Two]

Mixology

[@andikusnadi1979]

Using. Bleach, how many percentage to use?

[@FOGGYFOO]

One of the best videos I've seen for this process. Thanks

[@dianacoburn8269]

Thank you for making this easier for the novice low tec person

[@WildernessMedic]

I don't get the upside down part. Look how much the lid you're working on is bowing up and down every time you move. Wouldn't that be creating a ton of air movement in and out?

[@robertmclean9737]

What do you folks think about putting a Injection port in the Petri Dish? Cheers

[@Mizerka_]

Only if you're pouring your own agar and sterilising dishes

[@moonolyth]

I have wanted to do this for some time. Just a thought, Sodium hypochlorite is a great disinfectant you can find it in Pool cleaning areas Lab content would be 80% but the pool stuff is great for many things. Used to clean well water and food products in major industrial areas. Also a main ingredient in killing fungus growth in wood framing such as under houses and basements... .. . (Perhaps not needed). Something more, I use a similar box for jewelry work to capture dust I keep the box right side up and silicone a picture frame glass to the top so you can see better.

[@thetravelinglpn7092]

It’s 1cc not ten. The whole syringe is 10 ccs. This could have confused a lot of new people

[@jakewollin6262]

Thank you I was confused

[@SporeWhore661]

Got confused syringe is now in my ass, what do I do now?

[@biggerdreams369]

With this SAB, would you consider the environment to be semi-sterile as long as you didn't pull your arms out of the hole? If arms come out everything would need to be sterilized again correct?

[@Aaron-my8dl]

Thanks for the free knowledge

[@midnightmushroomco.2091]

My pleasure!

[@Nape830rigge]

I made a syringe from a spore print and put way too much spores in it. I innoculated grain jars and it grew in 9 days from spore syringe

[@Dylan-cy7cb]

How long does it take in agar if u use a spore syringe?

[@marandajenkins-wo3co]

I don’t understand please clear this up isn’t the air that’s in the box the same air as the room you are in??

[@CMZneu]

Great video! what type of markers are you using because mine rub of when sprayed with 70% ISO? are you using water and bleach? what %? does bleach not rub the marker off? do you prefer bleach or alcohol, if so why?

[@Enochulate88]

Why does everyone else put the needle back on. I was assuming the way your doing it was correct without ever doing it. thanks

[@biofoundry481]

At 20:15 you said 10cc's but I think you mean 1cc? 1cc=1ml (cc means cubic centimetres, aka 1L/1000)

[@Six_Foot_Two]

Yes, syringe is only marked to 10cc's

[@davidluedeke3805]

What temp. do you grow the plates, broth/liquid cultures and grain spawn? Do you store the broth and plates at 4C once you have the a culture grown in the medium and for how long do you store them at low temp?

[@sonicfanremixteam]

Always was told not to fire up a torch inside of a sab because it ruins the point of still air.

[@garlenkhajesari4366]

How about no rinse sanitizers?can be used instead of alcohol?

[@sjewitt22]

Is liquid mycelium just a older spore syringe? What's the difference in how they are made, and why would anyone choose a spore syringe if it takes longer to inoculate?

[@Redweezle]

Spore syringe is easily acquired. Liquid culture is normally made from an existing fruiting body through a process that can be found on KZbin. Liquid culture is a nutrient rich suspension medium while a spore syringe is just sterile liquid that spores float in until use...from what I have observed. I started with spore syringes simply because it was the "novice starting point". With more research I moved to liquid culture and agar transfer.

[@sjewitt22]

@@Redweezle Thanks

[@sjewitt22]

@@Redweezle Is the reason people use a substrate for price reasons? Or is there another reason people don't just use a huge bag of grains for example and fruit using the spawn medium?

[@kimberlydidia39]

I noticed your agar plates had condensation on them when you placed the spores on them and then sealed with the tape. How are they then stored? What temp? And the condensation is ok or does it need to be eliminated somehow? Thanks for the great videos!

[@SketchybrainD]

I put pvc rings on my SAB that zip lock bags fit super tight on that act as sleeves so just my gloved hands and bags go in and it sits on a stainless steel surface. The bags are probably overkill but it sounded good at the time

[@WhoDeyWildcatsReds]

Do you need to pull the liquid that is still in the needle back inside the syringe? I think i contaminated a syringe from improper technique after using and storing my needle

[@lucasantonini7618]

Why do people always put the box used to make SAB upside down? Thanks for the video, nice!

[@HeyHeyHarmonicaLuke]

They tend to be tapered and wider at the lid than the base. So flipping them gives you the most table area inside them

[@lucasantonini7618]

@@HeyHeyHarmonicaLuke Humm, thats true! Thanks

[@Enochulate88]

When it happens in the middle I do a break and shake. It works like a charm.

[@Enochulate88]

#dontstabyoself

[@QBDad18]

How do you store the surringe culture if you don't use it all?

[@jongreen2311]

Hey, I'm going to make one of these but I was going to connect gloves but then seen what you said of it creating a billow effect pulling air in from the lid.. surely of my container has an air tight lid then that will avoid that?

[@jidun9478]

Really good work, thank you I really like your channel here, wish you had more🙂

[@midnightmushroomco.2091]

Thanks!! I'm working on some more shorter videos about techniques and procedures I use. Simple stuff that keeps my processes going without too many issues.

[@MDinhh]

Would you say this is possible?: Agar > Grain bag > 10 Grain bags > 100 bags > 1000 bags

[@midnightmushroomco.2091]

That's absolutely possible! Mycelium expansion increases exponentially. You could then turn that into thousands of pounds of fresh mushrooms given enough space and resources.

[@MDinhh]

@@midnightmushroomco.2091 Thanks for the reply!

[@miguelsuarez-solis5027]

I read somewhere that keeping the SAB a bit moist helps fight contam as any spores that find their way in there can get caught in the condensation

[@burymebelowawillowtree9243]

So you just spray water inside?

[@miguelsuarez-solis5027]

@@burymebelowawillowtree9243 I spray isopropyl inside... Keeps it moist and clean... If I'm gonna work with flame then Clorox because I don't want a boom lol

[@burymebelowawillowtree9243]

@@miguelsuarez-solis5027 thanks!

[@carlfranz]

@@miguelsuarez-solis5027 What mix of Clorox and water do you use?

[@miguelsuarez-solis5027]

@@carlfranz 2 tsp of Clorox to 2 cups of water

[@贾信息一]

I really like your work station , did you buy it somewhere or you built it you own? anyway I can get one for myself ?

[@midnightmushroomco.2091]

It’s from a lab equipment company called GermFree. I bought it on auction from a university

[@michaelab2549]

what ratio of bleach do you use with water?

[@buttninjaflex]

he said in the video to use a 50% bleach/water solution (1:1 ratio); I highly recommend NOT doing that as it's noxious. 1:10 bleach to water ratio is sufficient. You can google around for bleach concentrations needed for sanitizing. They'll often use even less than 1:10.

[@Mr.Gandy.]

Vielen Dank

[@keepemwet]

I think you meant 1cc x2, not 10cc twice

[@aidennymes6335]

those drops of ms solution went all over the place while you were wrapping the parafilm

[@SillyWabbit120]

So I’m lost! What can you do with the Agar when it’s fully inoculated?

[@midnightmushroomco.2091]

You would then cut up the agar with a sterile knife or scalpel and put into grain.

[@SillyWabbit120]

Hey How much of the agar would you use for a 3lb bag of Grain? I’m in the middle of Putting an Order together on you site..Thanks

[@CaptMang]

Does it matter if the parafilm is sterile? I noticed they were pre cut, meaning they definitely have contam. I guess that doesn't matter?

[@chrisknechtel6730]

ideally the cut sides of the parafilm never come close to the opening of the containers... They stay closed, you can contaminate the outside surface all you want, the parafilm keeps it closed anyway. Never had a problem of contamination going across or through parafilm if that's what you're worried about

[@hopperthemarxist8533]

I notice you didn’t flame torch the needle is that not necessary to do when injecting? I suppose the needle is already sterilized right out of the wrapper ?

[@midnightmushroomco.2091]

Correct! They are typically gamma irradiated by the manufacturer. That’s why nurses don’t flame sterilize before giving you a shot. 🙂

[@CanadianManDan]

How long after sealing them would you inoculate it? The residual moisture left when it cools after shaking leaves grains wet. Would you let the bags sit a few days or use them.

[@midnightmushroomco.2091]

I’ve never had that be an issue. The moisture is drawn into the grain in about a day but I use them as soon as they cool down.

[@SillyWabbit120]

Great Informational video! Thanks

[@Donnydarko9912]

I worry about trying to grow mushrooms in a home where my dog is and there’s really no place that he doesn’t go what do you think about that

[@amitdebnath9625]

Loved the information sir!! ❤️

[@justinshivers261]

first grow. tried innoculating agar however, using the syringe I put way more than just a drop. did I done goof? I got extra agar just for contam so I could do agar to agar if need be.

[@createurimage]

A still air box can replace a flow hood completely? Thank you.

[@romeo5239]

Can you spray your arms with the alcohol to sterilize them?

[@fantastico4795]

Mi cepa perdió mucha calidad ¿ como puedo mejorarla ?

[@Enochulate88]

No inoculation loop... Just straight up drop it... Lit. I still got a loop but I can do this also .

[@Enochulate88]

Thanks definitely go to

[@chicofromph33nix64]

How much LC would you recommend for a 3lb grow bag...

[@justinlemma134]

When you use a lighter inside, doesn’t it pull dirty air in from the outside and create air movement?

[@luciferslettuce]

Yes, the SAB minimizes the amout of airflow though.

[@jgredy7]

would too much bleach solution inside the work area possibly get into things and cause problems? i understand the flammability aspect, but at least the alcohol evaporates away? i am new to this thanks for input!

[@Blue-bf8lv]

I can't see why it would be an issue, with bleach, more the better

[@claravale1756]

Do you not have to dry the bleach once it’s been sanitised?

[@stephenwarrington9719]

You know him he doesn't like extra steps.😄

[@TheKinderOne]

Dude you look exactly look Julian from Trailer Park Boys. Great Info Though!

[@midnightmushroomco.2091]

Frig off Ricky!!

[@UMepher]

Wonder why he hasn't picked up his glass once.

[@christophermccurry8039]

If you are like my wife and allergic to bleach, dilute ammonia can also be used. Smells worse but kills mold all the same.

[@Washed_gamer]

Julian from tpb at it again

[@midnightmushroomco.2091]

Frig off Ricky!!!

[@AngieCee1]

😂

[@milesgrande8271]

So if you aren’t cleaning the air inside the box with Lysol or something how is that helping? Would it be any difffetwnt then cleaning the supplies with alcohol then doing it open air? The air inside is just as full of spores then the air outside I would assume right?? I’m actually wondering not trying to be an ass.

[@midnightmushroomco.2091]

That’s a very valid question. In the open air there’s still air currents that carry spores and things around. Inside the still air box we try to let things settle and hopefully keep contaminants at a minimum.

[@bartbrinkmann8101]

Great video. The main reason I haven't jumped into a still air box is that I don't want to do the nuclear method of 4" PVC into thick rubber gloves. I like your method of just sliding clean hands through the ports. Do you find that this still gives a pretty good success rate?

[@midnightmushroomco.2091]

Actually, a lot of people unknowingly set themselves up for failure installing thick gloves into the box. Gloves create a billows effect that pulls in air from around the lid of the SAB. So when you move your arms around with the gloves installed you’ll literally pulling in dirty air from outside the box. Having no gloves avoids this all together.

[@jongreen2311]

@@midnightmushroomco.2091 What if the lid is air tight? Does this still apply?

[@sherwoodforestcoffeeestate8151]

Is the tape you stick onto the bag just regular tape? Micropore tape or something else?

[@TVideoupload]

He used normal tape there, I believe his bag had an air exchange port up at the top but I’m not sure if it’s needed or not.( my guess is yes)

[@midnightmushroomco.2091]

It's literally just regular packing tape. I've used injection ports but this honestly works just as well.

[@saviorlife1]

Should we spray the inside of the box when it's closed with Lysol to clean the air inside the Box

[@NoName-dd5vq]

Lysol is more flammable.

[@SillyWabbit120]

Hey What size Tub is that?

[@jwild666]

how do you grow paneullus stipticus? just colonize grain and cut sides of the bag?

[@jameskenna1324]

I get my from an online store, they got shroom, psychedelic and microdosing products well refined, also ships to anywhere.

[@jameskenna1324]

Mycopete.

[@luciferslettuce]

Was that a spore syringe to agar transfer or was it live mycelium from liquidculture? I've tried LQ to agar before to see if it would work and haven't had any luck. Have you?

[@StanWatt.]

What size (in litres, plz) is your SAB? Oh, btw, I subbed :)

[@smallybiggzzz]

It looks like it's around 88 quarts. You want somewhere between 80quarts to 120quarts max

[@Realistone69]

How do you liquidize the culture?

[@davideluciano7761]

Where u stored the syringe

[@UMepher]

Prison wallet

[@jakewollin6262]

How many ccs do you inject?

[@lukec2226]

I understood the iso alcohol solution was sterile once dry? You are spraying around like a fountain in that box.

[@RoCkShaDoWWaLkEr]

Working inside a SAB you want the surfaces wet to hold down any potential airborne contaminates, in front of a LFH you want dry surfaces so airborne contaminant blow right by.

[@Purity262]

just use paper towel as the cloth?

[@LongDefiant]

Do you recommend using a cell spreader for the liquid culture?

[@midnightmushroomco.2091]

You could but I never do.

[@penelopedrawsstuff]

Everything is dripping wet with the bleach solution - how does this not kill off the spores?

[@midnightmushroomco.2091]

That’s exactly what you want it to do. Molds have spores. I’m not getting the bleach in the agar or bag. It’s simply to kill any mold or bacteria in the outside of the plates and bags.

[@penelopedrawsstuff]

I see. Still learning here. I would be worried about it dripping from my gloves or rolling off the lid or something.

[@seanmoran6683]

So I've always heard that the best mix of bleach and water for sterilization is 10/90. Is there a reason why you should use less or more?

[@toddwmac]

You heard right and he is a bit off on a couple things. f you look at the label on any cleaner it will give you the optimal ratios. If someone does not believe the label, ask yourself this...why would they lower a ratio when increasing the raito would have you using more product and earning them more revenue. It makes no sense. Generally they will give you the best mix for the job at hand, One change to his video would be to let the bleach mixture dry. For the most part, none of these compounds kill on contact, they need time to penetrate the organism to kill it. Just like alcohol, you need to let the material sit for a bit before you wipe it down...if you do at all. In SAB work and when it comes to contam, slowing down a bit will go a long way.

[@oddbod60]

rare roachdoggjr spotted

[@twoody6505]

Do you buy pre poured dishes?

[@OurKax]

HELLO midnight mushroom - thanks a lot for this great video! what is the exact brand you used for the SAB trying to find it online. TY

[@midnightmushroomco.2091]

It's literally just a plastic tote from Walmart and I used a 4" hole saw to cut the holes.

[@jidun9478]

Yeah they are really overpriced online much better deals at the big box stores.

[@whyunosubcribe433]

What is SAB?

[@danaclass]

@@whyunosubcribe433 Still air box

[@michaellounds8295]

so the sab with the fixed gloves arent necessary? filering the air too much prior to work?

[@midnightmushroomco.2091]

A SAB with attached gloves are actually worse. The gloves create a billows effect and will suck in air around the lid of the box. This completely defeats the purpose of a still air box.

[@michaellounds8295]

@@midnightmushroomco.2091 thanks

[@InesAzaiez]

Hi thanks for the video! What is the size of the holes please?

[@emilys2849]

4”

[@midnightmushroomco.2091]

Right, they are 4" holes. Just big enough to put your hand in.

[@stephenwarrington9719]

Thanks for the video dude!

[@anthonyroman131]

Is that a 66qt box?

[@johnbermudez9932]

Just Switched from still air box to flow hood. What would you say is better? I’ve had zero contamination with my still air box, but you have me worried about using my flow hood.

[@davidluedeke3805]

A flow hood is much better and you can likely use alcohol in it. The reason alcohol is bad in a SAB is the alcohol evaporates and sits in the box. Add a flame and BOOM! Well a gush at least. In a flow hood the vapor is pushed out.

[@Paintingtube]

How much amount of bleach do you mix with water into full amount in bottle spray?

[@sedaeng]

thought he just said 50/50 in the video

[@Paintingtube]

@@sedaeng thank you sir 👍🏼

[@jameskenna1324]

He's on Instagram with the below handle..

[@jameskenna1324]

Mycopete.

[@hillbillyherb]

Great demo!

[@whyunosubcribe433]

Is that grain pasteurized in some way?

[@midnightmushroomco.2091]

It’s sterilized. Check my channel for a video on how to easily make grain spawn.

[@wallmark321]

Great video!

[@midnightmushroomco.2091]

Glad you enjoyed it!

[@Platypus2012]

Bleach takes time to work. Alcohol is fast acting. They work completely differently. Ideally bleach first, let dry, then alcohol to sterilize as you go. But it all works if you stay clean as you said.

[@k-lab3824]

Alcohol sanitises it's doesn't sterilise.

[@Platypus2012]

@@k-lab3824 Thank you. Sanitize not sterilize.

[@dougtan9429]

It looks like you remove your right hand from the box several times to dispose of the parfintape....

[@edieseltzer4896]

How long does an inoculated agar dish live?

[@midnightmushroomco.2091]

It can take about 2-6 weeks for the fungus to grow out on the plate. If there are no signs of contamination I'll move them to the fridge. They can easily last 6 months in there. The only real issue is the agar drying out.

[@rico0402]

do u turn on ur flow hood when u do this

[@midnightmushroomco.2091]

Yes absolutely!

[@nikkifigueroa4441]

What if in don't have one? Flow hood*

[@c4ljr221]

@@nikkifigueroa4441 if you just growing for fun here and there you don’t necessarily need one, the still air box a great idea but if you have a sterile work area with little air movement and take the precautions needed should be able to grow some mushrooms

[@Paintingtube]

Thank you sir 🙏🏼

[@cezarykowalski6849]

what did You add to agar cause its blue?

[@midnightmushroomco.2091]

It's literally just blue food coloring. You can use any color you'd like.

[@brandonhoward5659]

Why 50/50 bleach? Isn't a 10-to-1 mixture sufficient?

[@midnightmushroomco.2091]

It's honestly just personal preference. 10:1 works fine. Just test and see what works for you.

[@bigbrotheriswatching2680]

1 : 32 ratio is effective, that's 2 tablespoons per quart (a quart is 64 tablespoons) for disinfectant use. I like to go with 5-10% which would be about 1 : 16 or 4 tablespoons per quart.

[@Allyourbase1990]

I’ve been considering making one of these . Until I have the money to buy it make one , does a small Hepa air filter work? And also do you keep the grain in the still air box until it’s fully colonized ?

[@omarcuevas3048]

It would be best to keep the bag in a dark and warm space. You want to find out what temperature is best for that particular mushroom but it's also pretty easy to create your own incubation chamber that allows you to control the temperature.

[@HellGod67]

Not much of still air if your box sides are constantly flapping open.

[@foxwonderland8467]

Great video thank you. I watch a lot of these videos and I enjoy your style. 🙏 Question, with 1/10ml syringe of liquid culture how many 5 pound bags of grain spawn can I inoculate? Some people say two bags so 10lbs of grain spawn and other people say they use very little and find great success with only using 2ml per 5lbs of grain spawn. So the 1/10ml liquid culture gives them 5/5lbs of grain spawn. Now that seems like too little to me and my next question was did those bags take forever to fully colonize? Or was it similar in time to how long day 2 bags so 5ml in each 5lbs bag took to fully colonize. But they never got back to me with the answer :(I understand people are busy and I just appreciate any advise I was given!:). So I was wondering what your take is? Also if I may ask another question, how much of a ready agar plate would you cut up and put into grain spawn? I know to use the nice middle sections that look the best, but to me I feel like I could use each agar/Petri dish for a few, maybe 2-4 bags 5lbs bags per Petri dish. Any thoughts? 🙏🤗🤩🙏🇨🇦🙏THANK YOU FOR ANY HELP YOU WANT TO SHARE WITH ME🙏🙏🙏🙏🙏

[@kabutomushi-san8977]

Hi there. How many liters is that box

[@midnightmushroomco.2091]

I believe it's a 62L box.

[@joshuahall8268]

Damn. Thought it was Julian from trailer park boys doing this video.

[@Ryanizer1000]

Another move to add a layer of protection is to take a pair of large, rubber kitchen gloves, glue the rim of them around the hole and cut out the hand portion so you can stick your hand through, this way it will reduce the airflow into the box while you are working while not inhibiting your hands as much as in a traditional glove box

[@midnightmushroomco.2091]

Adding gloves actually causes a billow effect and will stuck in air around the lid and possibly pull in contaminants.

[@addammadd]

@@midnightmushroomco.2091 how about a tyvek filtered port to ensure no vacuum possible. Seem like a good move? (I’ve never done this, I’m just on my like 8th SAB video lol, 9 more and maybe I’ll make of these)