Inoculating Grain to Grain - Making Mushroom Spawn

Quick lesson today on grain to grain transfers.
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[@davidafenderson3446]

After watching your videos for the past month I have successfully created my own: SAB Spore syringes (totally sterile) Agar plates done with your solo cup idea Growing spores on agar (totally sterile) Isolating my first genetics Successfully developed my own unique recipe of grain I have a shelf full of about 30 jars with mycelium running like Bolt Spawned three shoebox tubs and it all looks great. Thank you sensei, I really appreciate you taking the time to collect all this information and show us, step by step, how easy it is. Once I have extra funds I’m going to support the Patreon. 🍄💛

[@morghan_glamour8214]

Do you know what technique he used to colonize the first jar???

[@Enochulate88]

Good job fam

[@timothywallace5507]

@@morghan_glamour8214 agar to to bird seed

[@0psec_not_good]

Good stuff man! Keep at it!

[@Gingerbred_Hed]

Its because of you and the rookie mycologist that gave me confidence to grow my own medicine. Youre doing gods work. Keep it up!

[@PhillyGoldenTeacher]

🙏🍄

[@zeezaweth6088]

One main critique: Do ALL your shaking OUTSIDE of the box. Get your primary spawn jar broken up then place into box. Once you add the spawn to the next jars, just tighten the lid and set aside. As always, keep movement to a minimum inside the SAB. Once you have done all the jars, pull them from the SAB and spread that spawn. One thing I love about starting this hobby is there are hundreds of way to go about things. I am always learning and trying to dial in my own tek. Thanks again for another great video!

[@markl4287]

Do you crack lid a 1/4 turn to allow some gas in?

[@donuts_are_good]

yup

[@thorny3218]

He working with a laminar flow 🤣

[@darkangel686868]

PGT, you make it look so simple, you’re a true pro!

[@calebisenor6292]

It doesn't have to be complicated. The simpler the better.

[@craig265]

I'm a pro ... But you will have to ask for me on broadway Friday night...

[@shawnwillis767]

It is simple!!

[@chandracox6814]

It is simple.

[@philosophyman]

This is THE most important video for bulk

[@mmpatriot2170]

So simple. I didn’t even know this was possible. Thanks!

[@chadwalston28]

Literally the best my dude. You give me confidence humanity will make it.

[@smokenjoe001]

No sir, THANK YOU for all your wonderful helpful videos and instructions. You truly are incredibly good at teaching.

[@jamesdubois2129]

Im going to send my support when everything comes to fruition. Love your videos man very well put together

[@wheatonna]

It's crazy how your 90% colonized jar looks so much less colonized after the shake-shake.

[@VladTheImpalerTepesIII]

That's because technically it was only 20% colonized if that. A fully colonized jar of grain cannot be shaken loose, you have to go at it with a spoon (sterile) and work at scooping it out. If you can shake it loose and see grain, it's not ready yet for G2G and certainly not ready for spawn to bulk.

[@LarryBird_]

@@VladTheImpalerTepesIIIthis is not true 😂 If you cant shake your grain loose before S2B you have bad spawn

[@frotob3816]

i’ve never had so much fun learning something. your videos make the flood of various contradictory information seem very much more digestible considering i have this baseline to refer to, giving me the confidence to start and learn more as i continue this journey. Thank you!

[@raulduke1528]

Hey your my favorite teacher.

[@PhillyGoldenTeacher]

Hey you're an amazing part of the community! 😁

[@donkulasmushroomsandagri3123]

Sir PGT 👍🍄

[@EarthPoweredHippie]

Thanks for everything bro, ur channel is so useful and well explained, as long as u put out these videos for the community I'll be here to learn.

[@PhillyGoldenTeacher]

Thank you! I wouldn't be able to do this without you and everyones support in this wonderful community. I'm glad you are a part of this journey! 😁

[@levihinsen1917]

Best content out there Philly🔥

[@Kalashnikov762x39AK]

Definitely gonna try this for my next inoculation ! Thanks for the info!

[@barryspodge6972]

Another straight to the point video thank you

[@Loumoli954]

Thank you for sharing your knowledge!

[@joeyonelove4708]

Thanks for the content PGT! Always look forward to a new video from the sensei! Damn look at that patreon list grow man! Come a long way since what 2 or 4 names on that jawn. Keep on keepin on brother!

[@connerwatson5818]

couldn't get to sleep and woke up to this beauty, keep up the awesomeness!

[@mikehoncho2149]

Same

[@jay7282]

Works great turned 3 half pints into 7 quarts for me

[@cybergimp]

awesome! i have exactly 3 half pint jars colonizing and was wondering how far i could stretch them.

[@mr.b9127]

Thanks PGT!

[@budlight9308]

perfect timing, thanks for great information, mush appreciated 🍄✌️

[@breathinginsilence]

love a good surprise video!!

[@sage5757]

Loving you're video's there helping my new hobby so much thank you sir🤘😛

[@mitchelllott]

big thx phillyG!

[@tylerliberty4162]

Thanks for these videos 🙏

[@benreed4918]

I did bird seed to Rye grain got full Colonisation I a week

[@forestbracht5278]

Very helpful!

[@MisterBones223]

Excellent work! I'm a recent subscriber, and I'm fascinated with mycology. Thanks for giving us this information!

[@tonyman1030]

Froggo doggo? More like Froggo Druggo 💪🏻😎🍄

[@chronomeatbag4223]

iv found its worth adding a few layers of 1-3mm vermiculite in LC grain jars if you dont shake during colonization you end up with so many tiny inoculation points when you use it for g2g.

[@jakemertz2660]

Wow this gives me so much to think about!

[@PhillyGoldenTeacher]

😁 Learning is fun

[@Mohammadparvazi]

Awesome this was actually a great lesson. I learned a lot from you and this is my second day of leaving my bulk substrate and spawn in a monotub to give me mushrooms. Thanks

[@PhillyGoldenTeacher]

I'm glad it helped! I wish you the best on your journey 🙏 🍄

[@shadyshrooms9056]

Respect Sensei

[@kingmetroplex]

Brilliant

[@digdug6515]

TY Philly ☮️💕🍄

[@bigpimpin6085]

1:47 god damn that was a smooth transition

[@l0lan00b3]

Literally had me thinking he pulled another jar from the side lolol

[@AngelAnge1]

Amazing so easy! Thank you ❤️

[@PhillyGoldenTeacher]

Good luck on your journey! 😄

[@ivan3465]

Thx❤️

[@terrymccorry8849]

Ty

[@ripme6616]

Mad!!

[@entityknownaszlap7298]

God man you have such great thumbnails

[@bigtank222g4]

PGT can you do a class on isolating to monoculture. Having spotty harvest. Genetics suck. Thanks brother

[@douglasmolik8069]

Been there, keep @ it brother

[@MikeWMiller]

Always love the videos and the quality. Just one question -- is the wire cooling rack you always use in your SAB actually doing anything? Assuming you wipe down the bottom of your SAB as well as the sides when you begin, it seems like it does nothing except introduce instability to whatever you set on it...?

[@PhillyGoldenTeacher]

it provides an elevated surface to work on, and in my case it does provide stability because im using the lid of the SAB as the base and its flimsy and not really a true flat surface.

[@mgdpublic]

Hey PGT, have you had any increase in contam rates with those unmodded lids? How do you take them out of PC - Do you wait for them to cool before opening PC so contamned air can't get sucked in before you screw the top? I made some screw top no pours and was stunned when they triched out in the few minutes they were a quarter turn loose when I pulled from PC.

[@williamwesley5078]

Great, my friend just did a g2g and its working! Do you count it as a 2nd Generation Mycelium because of its Maturaty. And is it a good way to Isolate a Specific Strain? This is my friends new fav hobby, he said it's quite easy once you know what your doing. Hes on his first bulk grow, thx for knowledge

[@kkrollingskkrollings3173]

At this point do you leave the jars cracked a little to allow some oxygen in, i have watched most of your videos and i just found u and subbed, i think u said in a earlier video to keep open lid a liitle like a quarter turn or something but i dont remember for sure, as i have a bad memeroy. Thanks .

[@gregoriotoscani9964]

When you works with agar and jars you use a Still air box or a Laminar flow hood?

[@irjeco]

Sensei! I love your vids this is my first time growing I have one question though, I inoculated two bags on the same day and kept them next to each other and one of them is doing so good already spreading mycelium and the other one looks like is doing some sort of oxygen exchange and its holding a stable humidity but I'm not seeing anything at all. Is this normal?

[@metaglypto]

Newbie mushroom grower here. I have played around a bit with those mushroom blocks you buy to grow mushrooms. I was wondering if anyone has experimented with using those blocks after one or two fruitings, as an inoculant for another batch of mushrooms. I was thinking of using a 5 gallon bucket, with holes, of fresh substrate and an old "log" crumbled up for an inoculant. Anyone tried this, and if so, how did it work?

[@devinshilightsdale9738]

Why do fully colonized jars look so uncolonized when you break it up?

[@overzone666]

Mycelium is microscopic :D

[@spewdog]

I think the mycellium bruises and changes color when it is broken up and turns white again after a few days. Maybe PhillyGoldenTeacher can elaborate

[@stephenodubhlaoich]

​@@spewdog Nah it's just because it's so fluffy, like cotton candy. A thick fully colonized agar plate will become just a piece of material around the size of a grain of rice or smaller on your finger if you swipe your finger around the whole thing. But it leaves small amounts of mycelium everywhere it already was before the shake and after it had already eaten from the grain so it regenerates easier.

[@VladTheImpalerTepesIII]

Because it's not actually fully colonized ;). A fully colonized jar of grain cannot be shaken loose, you have to go at it with a spoon (sterile) and work at scooping it out. If you can shake it loose and see grain, it's not ready yet for G2G and certainly not ready for spawn to bulk.

[@stephenodubhlaoich]

@@VladTheImpalerTepesIII That's not true though. Mycelium grows outward every direction if it isn't on a 2d surface like agar. If the jar looks fully colonized from the outside of the jar, it's good on the inside too, for a transfer at least. A jar doesn't need to be consolidated so long that it refuses to break up, to be considered fully colonized. Neither me or anyone I know, including professional growers, let it get to that point before G2G. Although I do let them sit a few days or so after fully colonizing, to consolidate, before spawning. There's a difference between the two. Colonize just means when it's covered the grain, consolidate is when it has more time to digest it, and it gets thicker during this time. Also pouring grain for G2G is better than a spoon, so shaking it is something you should be able to do. But if you're not waiting a while for your jars to consolidate like that, and they're still hard to break up, it could be a sign of bacteria, which normally will still fruit, but not to it's potential.

[@intergalacticprophylactic]

ALL HAIL!

[@jk-ed3pi]

nice

[@afinedayforfinearts9670]

Can you do a video on reusing substrate?

[@lucacavalieri2735]

Hello Professor! Due to money and space, and having a grain spawn purchased online, I would go for a grain to grain transfer using 1,5 liters plastic bottles, do you think this could work? Thanks for your knowlede, always inspiring!

[@tommyguntommy9816]

Is there a way to extend the shelf life of a “donator” jar of inoculated grain? Such as in a way where this donor material can sit for months and be used from time to time without the mycelium expiring from lack of nutrition. Can this be achieved by a fridge? Or adding food from time to time? Thanks for any thoughts on this

[@MrCowboyjeff]

My question is that I'm about to transfer a Uncle Bens Rice bag to 7 Qt. jars of Oats. My Oats expanded a lot more than I was hoping. Would it be a bad idea to spoon out a couple inches of the oats before I add the rice? I am working in a SAB.

[@Itswiggs93]

PGT, can you make a video on popcorn tek!?

[@khari83637]

I have UB bag that has colonized grain on top that I want to use for g2g. assuming i use a SAB and sanitize properly - can I transfer uncolonized rice from a freshly opened UB bag to a grain jar with GE lid and spawn from jar instead of using the manufacturers bag?

[@emrecelik4683]

Should the jars be totaly sealed or should they be little bit open? I forgot to drill holes in my. Thanks for answer

[@Shady-bp6es]

After a a jar fully colonizes can you place it in the fridge to store until ready to use?

[@sirbloodyrectum]

How do you grain and grain transfer with brf tel???

[@dillonjones1767]

Can this create an endless supply of mycelium? Once the 2nd one is fully developed spread that into new jars. Or will the process stop at some point?

[@axelnio7498]

Could i also use the content of my fruiting cake to colonize a new jar?

[@rosevaldez8820]

Do you have syringe inoculation video ❤️ from syringes ? What’s are YOUR basics ?

[@Hernand3zFam]

I'm new to mushrooms and I wanted to know could u use beans like pintos or another to use as my grain instead???

[@yung_dev1365]

I didn’t take any grain from my jars before transferring to bulk would I be able to use mycelium from the tub for a transfer to grain jars or should I just take mycelium from the fruits after harvest?

[@chrisd3372]

Do the jars your transferring to need a hole for air?

[@666grizwold]

Why many times when you are working in air still box you put your jars on this unstable metal crate? Is there some special reason for those crates? I'm anxious when watching that :D

[@spewdog]

PhillyGoldenTeacher, does the mycellium change color when you break it up?

[@richardview1281]

Love your videos. Do those plastic jar lids work just as well as the metal ones? My metal ones keep rusting and will switch over if it's all the same.

[@PhillyGoldenTeacher]

Yes I made a video about lids, check it out on my channel

[@richardview1281]

@@PhillyGoldenTeacher thanks, will do

[@t2grey578]

Lovely bacterial master grain you have there

[@muckimuck733]

No holes in the led needed? Can I do G2G without a still airbox? Thx

[@pgdenik3890]

Can i inoculate grain from already fruiting grow kit?

[@CindyyyToon]

This is great! I have one question though. Can I use any type of jar for this? And do the lids need to have breathing holes? The ones you used were fully sealed so I thought I should ask

[@frotob3816]

any clean jar will work. he keeps the jars loose a quarter turn for air :-)

[@darreno7602]

Would grain to grain transfer work if your colonized jar is essentially 90% colonized or is too far along to do so? I have an extra jar that wasn't ready at the time I moved the other jars to the sub. it has solid myc growth covering the entire jar and only a few grains are left to colonize

[@RyanWalsh83]

How many times can you do it? Like, if I used one of the new grain to grain to create more. Then a new one from that to create more?

[@frankdatank2304]

So If I have a fast colonizing mycellium will it still be fast after grain to grain? Also would that be considered a genetic trait?

[@user-dz9rz9dl6g]

Hey bro great channel, thank you for all great videos! I have one question, i noticed that you don't have any holes on the lids, doesn't need air exchange?

[@PhillyGoldenTeacher]

It's an unmodified method that leaves the lids a quarter turn loose for exchange. It's mentioned in 'The ultimate lid tek guide' video 🙏🍄

[@user-dz9rz9dl6g]

@@PhillyGoldenTeacher got it! Thanks for respond! Keep up the great videos!! Greetings from Greece!!

[@cookieboy7102]

Just ordered my laminar flow hood cant wait for all thats too come

[@adamweber2107]

Where did you end up ordering from?

[@cookieboy7102]

@@adamweber2107 you can get one at midwest grow kits.com its the bonsai 99.99% laminar flow hood

[@richmac6345]

Is it essential to do this in front of a laminar flow hood? Great video. Thank you

[@laurencerosenthalwinckler4439]

2 options: plastic box with 2 glove holes (cheap option with low space) or flow hood with H14 filter. You can try your luck soon after shower in a toilet without carpets that u just sanitized like a OCD mom spraying alcohol on all the walls with all the windows and doors of the house closed using a mouth mask. Out of those 3 options its just too risky to get contaminations.. Like.. Those spores are literally EVERYWHERE.

[@Jonnygurudesigns]

How long can I store a grain spawn jar in a dark closet space? What are the options for a longer term storage ? A few months? Any ody have an idea?

[@SacredBear777]

do you use manure for psyllicibin...or will coco work for it too and can i make this up a day in advancc before i inject spores

[@chessjohns6396]

PGT.im a noobie. are you doing this inside a sab? and since there are no ae holes in your lids once you transfer grain and shake it around do you then loosen lid a quarter turn ? thanx.

[@kembotmt]

PGT, Do you have any ideas of how I might maintain the room temperature once my substrate & grain go into my monotubs? My home sits at about 16 degrees Celsius (60F) throughout most of the day and night. Will that negatively affect my growth? Or just slow it down a little?

[@nicinightbird]

I had mine abort in the cold. I just got a lil heater from walmart :)

[@njsurfnskate20]

Love that wire rack to work on, is there a link you can provide? Thanks!

[@PhillyGoldenTeacher]

They're called baking racks amzn.to/36zCLGm

[@_Mav]

Great vid as always 🤙 Can you keep doing this G2G indefinitely?

[@PhillyGoldenTeacher]

You can for a few rounds, but not indefinitely. It will reach a point of senescence.

[@esolbdivad]

@@PhillyGoldenTeacher what does that mean tho. They just stop or they don't yield as much?

[@redmoose7227]

can you use the remnants of a basic growkit for this?

[@gep7175]

When you are having your grain colonize in jars, do you need to have any air exchange holes with polyfill or filter patches? Or can it stay completely sealed. I know a self seal injection port is good for liquid culture for injections.

[@dressedanddepressed2301]

You can technically get by without any holes or modifications on the lid but growth may/can stall. I would leave the lids loosened 1/4th turn and flipped upside down if you aren’t using any holes or mods. If you use mods then use the micropore d surgical paper tape or polyfill

[@alexravelo6522]

Can you do the same process with substrate?

[@cvictor8999]

looks to me every jar is hermetically shut hence no air exchange of any kind. mycelium growth does not need oxygen to happen?

[@martyduty2598]

This is why I think sealable bags are better than jars for your mycelium growth. It seems to make it alot more convenient to be removed when it's ready

[@C.ODubhlaoich-sp3to]

"don't put all your eggs in one basket"

[@Pandawea]

What kind of jars are those? How do You sterilize the grain un those?

[@juankast26]

No air exchange for colonozation? I see no holes in the new jara

[@sa3kes111]

What if your grain is fully colonised and the myc is to strong to break and shake, how would you do G2G then?

[@nickmarks9507]

Do the jars need FAE? Those lids don't have any holes?

[@Big-three492]

Do need to use a filter on the jar lids?

[@Obelisk_Isle]

Are your lids modified?

[@Rynorasaurus]

Lids tight or loosened a bit before you incubate?

[@witnessforchrist7778]

Can you keep doing grain to grain indefinitely? Thinking of it as sort of like a sourdough starter where you can continuously add to it and multiply it

[@julian-io5wl]

Technically you can but it makes sense to start from time to time from agar to makes sure that your culture is not contaminated.