Inoculating Liquid Bacterial Culture

[@addgene]

Addgene does not regularly monitor comments posted here, so we may not see your question immediately. We’d be happy to answer any questions sent to [e-mail] as soon as possible. Please include the name of the video along with any questions so our support team can help. Thanks!

[@jasonbattermann9982]

Why do so many education institutions call LB lysogeny broth, but companies use the term luria broth?

[@impulseSSJ]

luria is the person who created the formula.

[@akshitasoni445]

Should i use nutrient broth instead of LB for Bacterial DNA Isolation @Addgene

[@lovelysoni1829]

Nice video for kind information thanks again very much to you

[@TALKyLIKEENGLISH]

so we should after transformation: liquid medium --> plate --> liquid medium. What if we skip the first step?

[@nitishkumarprajapati7115]

How to culture Sporosarcina pasteurii Bacteria?? Pls make video....

[@jejeoluwatimilehin8918]

in a situation where we use gentamycin, kanamycin and rif why is this?

[@nicolabyy]

why do you need antibiotic?

[@koalasaw]

very helpful thank you

[@funny11744]

Please provide here the link with quantities of antibiotics for culture . Thanks

[@addgene]

Thanks for watching! You can find a chart of antibiotic concentrations here: www.addgene.org/protocols/inoculate-bacterial-culture/

[@janebond3263]

how to storage with glicerol?

[@impulseSSJ]

my mans streaked it so wrong. you cant scratch the agar like that

[@Mirracle74]

Elon Musk wants to inoculate the surface of Mars with H. sapien. I think that it’s a lousy idea because Mars isn’t the right growth medium for humans.

[@danna3922]

Why don’t we take back the tooth stick out of the medium ?

[@addgene]

Thanks for your question! Leaving the toothpick in the culture tube avoids having to stick something else in the tube to remove it (e.g. tweezers), which could lead to contamination. Removing the toothpick once you've finished growing the culture is less risky because any contaminant won't have time to grow.

[@anjalisharma314]

Sir I have to incubate an organic liquid of 50mg/L with a bacteria. Please tell me the procedure for it.

[@addgene]

Thank you for your question! You might find the protocol associated with this video helpful www.addgene.org/protocols/inoculate-bacterial-culture/

[@anjalisharma314]

@@addgene thanks for response sir but I want to know the degradation ability of a particular compound by a particular bacteria. Shall I apply the same procedure, that has been given in the link.?

[@addgene]

@@anjalisharma314 The protocol shared above is intended for growing up bacteria in liquid cultures and may be a good starting reference. However, Addgene does not perform functional experiments like the one you've described so we can't provide specific recommendations on the conditions you may need to use. Sorry we can't be of more direct assistance!

[@anjalisharma314]

@@addgene it's ok sir?

[@laxmikiruthikamanoharan1768]

Thank u

[@devanisasankan818]

👍👍

[@Gilbertblythe292]

2023🦠🦠🦠🦠🦠🦠🦠🦠🦠

[@Gilbertblythe292]

2023🦠🦠🦠🦠🦠🦠🦠🦠🦠