Intact Cell Measurements 1

[@munansangu]

@Alicia, Thank you so much for this video!!! it came at the right time for me! the way you explain the concepts is impeccable! have a lovely day!🙏🏽

[@annab4389]

Loving this! I am still learning, love your lectures, they are so nice and clear. I wanted to ask about cells in suspension. For my cell type (lymphocytes) I know that people adhere them and I was just wondering similarly to what you say how much this affects the readout. I gues I would just have to compare the values. This was very useful, also the experiments with pyruvate with and without oligomycin for total respiratory capacity. Extremely useful! Thank you so much!

[@aliciakowaltowski7470]

You can measure oxygen consumption in suspensions with an electrode system like an Oroboros respirometer or any other Clark-type electrode system.

[@donnyB7408]

I love this video, thanks for it. wasn't expecting to find a video with such explanations

[@jamix8752]

This is absolutely fantastic. Thank you so much!

[@tatianam.5221]

Loved it! Thank you so much for this lecture, it is saving my day.

[@mohadesehahmadi3031]

This video helped me a lot. Thank you so much.

[@prajaktasawant07]

Great explanation, thank you very much

[@danaakl7310]

Thank you for these infos, really helped me a lot!

[@junaidrehmani9829]

Hands down best video

[@spicesmiles]

Thank you!

[@user-pf6to1np3t]

THANK YOU SO MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!1

[@MsMascavo]

Muito bom. Salvou os irmão

[@caseses]

you saved my exam!

[@tannyk11]

This is hands-down one of the best videos out there explaining the mito-stress test!! also if we can combine both the mito stress test and glycolytic stress test/rate assay test together? Thank you so much.

[@aliciakowaltowski7470]

I´m planning a more comprehensive online course with a couple of colleagues next year. Keep an eye out for it.

[@evelinanikel8640]

Dear Prof. Kowaltovski, all your lectures are brilliant! Thank you very much for them! May I ask a question about the scheme for XF measurements from Bioenergetics book? According to the scheme, pH is increased when the sensor approaches to the cells. However, as far as I understand, it should be the opposite: if glycolysis occurs the acidification of extracellular medium will take place, thus pH should decrease. Could you please explain if I am mistaken? Thank you!

[@aliciakowaltowski7470]

Thanks! Not sure I know what scheme you mean, but probably you are looking at ECAR - extracellular acidification rate, not pH.

[@evelinanikel8640]

@@aliciakowaltowski7470 I mean a scheme of XF analysis you show at min 5 in this video. It is Fig 9.6a in Nicholls and Ferguson Bioenergetics book 4th Ed.

[@aliciakowaltowski7470]

@@evelinanikel8640 You are correct! I hadn´t noticed that scheme was marked as pH, not ECAR, in the book.

[@evelinanikel8640]

@@aliciakowaltowski7470 thank you so much!

[@sayantaghosh1289]

what is the significance of ECAR and how to determine it using glucose, oligomicin and deoxy glucose? can you please shed some light in this point ?