Isolated Mitochondria and Permeabilized Cells/Tissues 2

[@NareshKumar-yf5ur]

Very useful, thanks

[@marieeliseabiantoun3361]

Thank you. Which one would be the Leak State?

[@aliciakowaltowski7470]

State 4 is proportional to the proton leak.

[@liangyuhu7490]

Thanks so much for the video. I have a question regrading the detergent. Is it possible to titrate the saponin to determine the optimal permeabilization condition for mouse heart tissue? Thanks.

[@aliciakowaltowski7470]

Yes - there are published papers using saponin-permeabilized fibers.

[@NareshKumar-yf5ur]

How to calculate RCR for succinate?

[@aliciakowaltowski7470]

Same calculation with succinate as the added substrate.

[@mayakima4774]

why is isolating mitochondria be carried out under 0 to 4 degrees celsius

[@aliciakowaltowski7470]

To prevent degradation during isolation.