Introducing Monstera Deliciosa to Tissue Culture

[@SourLot]

i don't see any difference in this from a standard node propagation if at the end of the day you get a single plant from the node cutting

[@MiniatureChickenChannel]

The step he shows is just the introduction part of TC. Like an "insurance" step you'd take if you have an expensive plant that you want to make sure it survives in TC before hitting it with growth hormones.......from what I gather anyway 🤷🤷

[@kennethpaulferino4770]

Can you please make a simplified version of this for students? Badly needed for a school project. Thank you so much

[@weightlesterasmr8368]

Hi! Just a bit confused. Isn't it supposed to produce multiple shoots to be divided? What is the purpose of taking a cutting to produce one plant? Or is it to be transferred to a multiplication medium? Thank you very much

[@rjjr.1071]

Same observation..

[@MiniatureChickenChannel]

Hi, I think he's trying to stabilize the plant first into an "introduction" phase of TC with no PGR's. Later, the plant will be moved to multiplication media. But this first step, of not trimming the roots completely, is like insurance, so the plant can move over to using the medium for nutrient and become established before trying to stimulate with PGR's in the first step. I could be wrong...just what I'm gathering from other video's/reading. 🤷🤷

[@vanessaprice9152]

Same I need an updated explanation video

[@MsBawarka]

Great video thank you.

[@dewmontjerkins9886]

Skip all that and drop the original cutting with leaves in a jar of water.

[@elisakro8639]

hey, can’t the monstera be placed into the multiplication media from the beginning, can it?

[@XPrincess30]

Is it possible to do this with just a portion of the leaf?

[@PlantCellTechnology]

You can, but then you may need to tweak the sterilization process and everything based on the explant.

[@tessav7830]

So once it grows in the agar, it will grow multiple plants/nodes to do the process all over again? I’m confused what the steps are after the tissue culture is successful from the first node

[@PlantCellTechnology]

After explants are established, transfer them to shooting media for shoot formation, then rooting media for root formation, then move it to peat moss pot for acclimatization in the greenhouse (covered), then remove the cover but plants still need to be in the greenhouse, and then in the natural environment. This is the whole process of tissue culturing a plant.

[@navidfarkhondehpay1142]

Hi! How long would it take for the plant to start producing fenestrated leaves from tissue culture? Would it be a few months or closer to a few years? Thank you!

[@PlantCellTechnology]

Not sure, it has been in tissue culture for 4 months and it has three regular leaves.

[@navidfarkhondehpay1142]

@@PlantCellTechnology when do you plan on hardening it off and potting it up?

[@lonestartropicalstx]

I would say probably 3 or 4 years.

[@hasandua6739]

I have a questions, is uvc lamp affected media MS? I trying to make uvc lamp as sterilizator for my media (autoclave so expensive for me). I'm trying to culture with low budget, thanks

[@PlantCellTechnology]

You can use the oven for the same! We have made a video on the use of the oven to sterilize media that you can find on our channel.

[@kevinyan5384]

What is the reason for doing this? You sacrificed one plant in exchange for one plant. Or does this create multiple plants? Whats the difference between this and just taking a cutting?

[@FJ_Palacios09]

Once established, you can multiply the plant very quickly. Thousands of plants every month.

[@siimolen]

@@FJ_Palacios09 Thank you for this explanation - One question - Could you instead have taken smaller or other parts cuts of the Monstera instead? To get started with multiple plants right away? Or is that single part the only viable part for this plant?

[@FA-ft9sq]

Somebody correct me if I'm wrong, but the whole point of this is to establish the plant in sterile media (Initiation as many authors call it). Contamination of explant is your number one problem if starting from an established plant, iirc and starting seeds isn't really an option for aroids like M. deliciosa. The sterilization steps did not rinse the explant at the end so I'm assuming that the explant is soaked in something similar to ppm to kill any pathogens hiding in the nooks and crannies of the explant. Necrosis will happen but using thicker stems will ensure that enough tissue survive to get the plant going in a sterile environment. Once established, you can then use this explant for multiplication later. Im doing the same experiment on a M. adansonii.

[@joeygiordani8987]

@@FA-ft9sq what does iirc stand for? How is you monstera Adensonii coming?

[@MiniatureChickenChannel]

@@FA-ft9sq I agree...just for introduction "insurance" sort of speak.

[@canalbruna5935]

Sou do Brasil e amo demais essa planta.

[@PlantCellTechnology]

ótimo

[@dianaf1669]

Where do you find the media for buying?

[@PlantCellTechnology]

Plantcelltechnology.com Get 20% off using code NEWYEAR21 until January 5th.

[@PlantariumBKK]

the tissue touched to bottle, it will contaminated?

[@FJ_Palacios09]

No contamination so far after 52 days!

[@PlantCellTechnology]

@The OrangeGroveDales Absolutely! We will make a video update soon.

[@pflanzenpapa950]

Does this only work with the stem or does it work with a leaf piece? I want to try this with my monstera thai constellation

[@PlantCellTechnology]

Based on the type of explant, the sterilization process and culturing procedure might differ a bit. But, you can try on small scale to see if it works.

[@outloud_overthinking]

thanks for your effort, really cool vid

[@PlantCellTechnology]

Thank you so much. stay tuned to our channel for more videos like this.

[@rafaelpulido6946]

Did you use a specific media for Monstera? Or maybe normal MS media? Thanks for the video!

[@PlantCellTechnology]

Full strength MS media with no hormones, the goal was to get the plant established in tissue culture. After established, we can transfer the plant to multiplication media.

[@Josh-cq9op]

@@PlantCellTechnology what media did you use for the multiplication stage

[@shiva16]

@@PlantCellTechnology how long did it take till the entire culture could me multiplied in the flask you have it in ?

[@mengyewleong5028]

@@PlantCellTechnology How to make it multiply? What media to use for multiplication? Is hormones required? Thanks

[@halmiar8981]

Can you make video for alocasia and colocasia plant? Tq

[@PlantCellTechnology]

Sure! we'll try to bring that in soon.

[@s4210179]

Hi, do you think if i were to use vinegar (food grade) it will work the same?

[@j.t.masters1934]

You need cleaning vinegar. Regular, white vinegar consists of about 5% acetic acid and 95% water. On the other hand, cleaning vinegar has an acidity of 6%. That 1% more acidity makes it 20% stronger than white vinegar.

[@FJ_Palacios09]

Food grade vinegar also works just need to increase the concentration.

[@monsterahut2185]

@@FJ_Palacios09 how many ml of food grade vinegar will you suggest to replace the cleaning vinegar used in this video?

[@PlantCellTechnology]

Food grade vinegar is only 1% stronger than cleaning vinegar.

[@jay_moy]

Hello, I have watched this few months ago but I'm here again due to curiosity. Does tissue culture process makes the regular monster deliciosa turn into variegated one?

[@PlantCellTechnology]

Variations may occur in tissue culture plants due to genetic or in vitro conditions.

[@jay_moy]

@@PlantCellTechnology hi, thank you so much for answering my question, I’ve been looking for answers since last month but didn’t find any, even on Google. So, now I understand that variegations may occur randomly during plant tissue culture process. Thank you so much. ☺️😘

[@PlantCellTechnology]

@@jay_moy There are many reasons for variations in plants even during tissue culture.

[@DannyTillotson]

@@jay_moy It's not random. It's generically modified in a laboratory in Thailand. Hence the name Thai Constellation

[@jay_moy]

@@DannyTillotson I’m really excited interested on how Thai modifies the genes of the plants but seems like when searching it in the Internet--it is complicated.

[@monsterahut2185]

Hi, is it a must to use vented cap? and why?

[@PlantCellTechnology]

Vented caps are good for gas exchange. It prevents the accumulation of ethylene gas, which can cause the death of the plants or vitrification. Some plants are more susceptible than others, and most plants do not need a vented cap. However, with some plants are highly recommended, like Cannabis, hemp, or tobacco.

[@niyaskhan.s7385]

can u share this plant media preparation

[@PlantCellTechnology]

Hey Niyas! Check out our Beginner videos! Hope they help!

[@kennethpaulferino4770]

What media did you use?

[@MiniatureChickenChannel]

from his previous comment "Full strength MS media with no hormones, the goal was to get the plant established in tissue culture. After established, we can transfer the plant to multiplication media."

[@siimolen]

Could you instead have taken smaller or other parts cuts from the same branch instead? To get started with multiple plants right away? Or is that bottom part the only viable part for this plant?

[@PlantCellTechnology]

You need to use the apical meristem, and the easiest way is by using a small plant.

[@siimolen]

@@PlantCellTechnology Thank you! What would happen if you placed this explant straight into multiplication media?

[@MiniatureChickenChannel]

@@siimolen I would like to know the same....I assume he did this as insurance to make lessen the chance of the explant having issues???

[@lorenzginoroyo]

any update on the progress?

[@FJ_Palacios09]

I would also recommend following Plant Cell Technology on Instagram for more frequent updates.

[@RusticRaver]

I tought the advantage was that you could do that with only few cell so you could do many, after seeing this guy reducing a perfect cutting to nothing, I'm not sure what to think, this defeats purpose.

[@PlantCellTechnology]

Tissue culture offers many advantages than that. And, the bigger tissue you introduce, the higher the chances for successful induction for some plants. And, when you obtain callus or stem regeneration, divide it, and culture it again, and repeat. this way you obtain many plants but took the plant tissues from the mother plant only one time.

[@RusticRaver]

@@PlantCellTechnology thx for the info, actually this channel is packed with info, I did watch couple last night and really enjoyed it. Best channel I found in long time, thx for that.

[@PlantCellTechnology]

@@RusticRaver Thank you so much for your wonderful feedback. :)

[@qulzarseleb]

You didn't rise it in step did you?

[@FJ_Palacios09]

No rinse, I only used 1% bleah.

[@qulzarseleb]

Ad did you use ppm??

[@FJ_Palacios09]

@@qulzarseleb Yes, in the media. 2 ml/l.

[@qulzarseleb]

You add it before pH adjusted? And autocave it??

[@FJ_Palacios09]

Add after adjusting pH, but before autoclaving.

[@palmdaddy]

Every time He touches his watch he is contaminating his gloves..

[@vanniyo8988]

Can you eat this?

[@PlantCellTechnology]

Monstera is generally used for house decoration.

[@MiniatureChickenChannel]

That would be some expensive eatin 🤣🤣🤣

[@nilushikawijevardhana6383]

What is use medium culture to ex plant?

[@PlantCellTechnology]

The media is regular MS media. Once established, we are going to add hormones for multiplication: MS media with 25 g sucrose, 6 g Agar, 1 ml/l PPM, 1 ml/l BA, and 0.1 ml/l NAA.