Isolating a single spore under the microscope

This video shows the breakdown of how I isolate a single spore from a wet slide using a pipette. Enjoy and MUSHLOVE
Amscope link
AmScope - 40X-2500X LED Digital Binocular Compound Microscope
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[@robbyhanlon]

Gary, I’m pretty sure you are the only dude on KZbin doing this kind of in depth content. Believe me, I’ve searched. We appreciate it. I am wanting to understand the process of breeding more and there just isnt a lot of content out there.

[@FreshfromtheFarmFungi]

thanks for watching and following along! I am building up to a new method of breeding can’t wait to share! 🙏🏻🍄❤️

[@diegoramosg]

@@FreshfromtheFarmFungi great videos!!!!

[@philkennybr]

Is the new method out yet?

[@InconspicuousOwl]

This is the one I've been waiting for! Cx

[@tonystewart7624]

It's because Gary is playing with fire, isn't it? Lol

[@bennynutts16]

thanks for sharing Gary!

[@HChandler2010]

Now I gotta figure how to justify buying a microscope to my wife 🤣 she'll understand 🫡

[@triple_gem_shining]

Shit man if it's business purchase she doesn't really deserve a say 😂😂

[@AaronAlso]

Them is some skills, my hands just aren't steady enough.

[@moisesaramos3229]

Gary is finally showing his gangster stripes. Digging the metamorphosis, also I knew he wasn’t just some gourmet cultivator.

[@tonystewart7624]

Thank you for another great informative video Gary.

[@philkennybr]

Super cool technique!

[@amanitaocreata4401]

This is why you're becoming my favorite to learn from

[@FreshfromtheFarmFungi]

thanks for the kind words! MUSHLOVE

[@sporgymushrooms9656]

Great video Gary! This makes me wish someone would make an iso printer that could print single spores perfectly spaced out on agar plates. Would love to see a video about strengthening genetics and principles of inheritance.

[@FreshfromtheFarmFungi]

hah glad someone figured it out 😎 this is my exact project! Kind of… stay tuned once I get the funds I will work on this 👍

[@JUSTINOURSE]

Thank you so much for showing how to narrow a Pipette. I didn't know that this was possible till now.

[@FreshfromtheFarmFungi]

learned it in 6th grade chemistry class I am thankful to have had such a trusting instructor looking back on it! 🙏🏻🍄❤️

[@coloradodirtbike5930]

Such highly valued info that very few people ever show or even know how. You are a master Gary and your expertise and knowledge really is endless. Thank you for taking the time to teach others.

[@marlinmixon3004]

I watched your video where you divide a spore solution multiple times until you get a low enough spore concentration to easily isolate single spores. I thought a bit and you might be able to do the same thing with sticky tape. Make a spore print on the sticky side of sticky tape, then line up a bunch of sticky tapes in a row and touch the spore print side to a fresh piece of tape sticky side to sticky side and pull it apart which probably removes half of the spores then grab a fresh piece of sticky tape and touch the last piece of tape together with the new piece. Eventually you get down to single spores. Not sure if a single spore on a piece of sticky tape is useful but was an interesting thought exercise.

[@JimmyGreen-wx6gt]

Awesome video

[@Milkex]

MAP gas is sold in yellow, propane tanks are blue

[@evil8anana902]

Bro before the video even started I saw the spore print in the petri dish and I woke up my daughter saying "that's effing genius" lol.

[@FreshfromtheFarmFungi]

thanks! I have a large library and making them in dishes also helps organize 🙏🏻🍄❤️

[@evil8anana902]

@FreshfromtheFarmFungi I'm working on the organizational part myself. Do you happen to know is there is such a thing as a screw on lid for petri dishes? I hate having to tape everything up haha.

[@phenofarmseeds5280]

Awsome

[@ErikPfau]

After isolating a haploid, and growing it out on agar, and combining it with another haploid, will the entire monokaryon become a dikaryon? Or only where they fuse together? Can you make a transfer from the farthest side and it still be monokariotic or does information travel through and know it has mated?

[@syberphish]

Hey Gary, what's a good reference for identification based on size and other spore attributes?

[@tims-pt1yy]

Great video😁

[@ScottWConvid19]

What is the advantage of isolating single spores over allowing spores to randomly mate on a media plate or other nutritious matter? Is there a way to determine which spores will have better genetics?

[@FreshfromtheFarmFungi]

the advantage is that you can get more variance - with random selection the stronger genetic expression will prevail and you end up with similar genetics (not necessarily a bad thing)

[@benjamindejonge3624]

Omg when you think Einstein figured it all out , somebody comes and says hold my beer

[@kainophile5887]

I was wondering if anybody went through this kind of trouble to get haploid spores. Do you make a practice of doing this?

[@FreshfromtheFarmFungi]

Not really, I am making a better process and filming currently- it has been a couple years in the works I don’t enjoy tedious microscopy but I do enjoy making videos 🙏🏻🍄❤️

[@revolioclockbergjr8482]

Can this be achieved with a hypodermic needle?

[@FreshfromtheFarmFungi]

yes I think with a steady hand

[@MonsterMushhead]

Is an isolated spore syringe liquid culture?

[@FreshfromtheFarmFungi]

no liquid culture is different- watch this video it may help kzbin.info/www/bejne/rmLFYpt7jZ10eK8si=5iM4QRnXMocRt_GM

[@triple_gem_shining]

Bro!!!

[@nubletten]

First time i hear about this is from you. Imagining a cross between cordycep militaris and lions mane that grow very well in wood and eat pests. Also it would look like a cordycep stalk with lions mane cap or reverse :)) This have crazy potential imo.

[@FreshfromtheFarmFungi]

hah yes but would be very difficult to cross ascospore with a basidiospore - genetic modification required 🧏‍♂️

[@MrEiht]

It was valentines day...did you let it meet is honey? Or is it still lonely?

[@LarsLarsen77]

Bro, it's a MICROscope, you only need one drop. Not 5.

[@renasdupont2806]

was hoping for mor than 100x

[@FreshfromtheFarmFungi]

that would require an incredibly steady hand haha but challenge accepted!

[@renasdupont2806]

true lol@@FreshfromtheFarmFungi