DNA cloning and recombinant DNA | Biomolecules | MCAT | Khan Academy

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Introduction to DNA cloning.
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[@samuelfrancis8574]

I just realised.... these vids don’t have ads.... they still aren’t making no money off this.. god bless

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yes thanks to not making any money we are successfully homeless .

[@Ariadne4]

What's wrong with making money off these tho? There are many useless videos making money off them, so why don't useful videos like these make money for the hardworking content creators?

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Yeah, truely one of the best Not for profit organisations out there

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​@@Ariadne4 He's not saying ads are bad, he's saying no ads are good

[@sajidabrar1]

Live Wikipedia

[@aileniirina3335]

Sir, you are my favourite teacher on the Khan Academy platform. Great job !!! You helped me a lot during my studies

[@emmanuelessel8650]

Thank you sir

[@tarimandeliko2672]

this is amazing. i have tried so hard to understand this (i have watched other videos, read an excerpt from a textbook etc.) and now i finally get it. thank you khan academy

[@unmceopcomplab3162]

Great video, Khan Academy never fails to clear up difficult topics/processes.

[@Unknowngamerborivt]

how is he so smooth with the mouse?

[@stitthappens]

he is using a drawing tablet ! :)

[@TheHiddenChronicle-b9l]

Bruh

[@rundown3984]

That’s what I thought

[@samratgowda]

Ohh, man he is using a writing pad.

[@behindthecurrent9147]

Experience *pen

[@nicolasgobbi5620]

Even 7 years later, he's still the best teacher to ever walk this Earth. Thank you so much for helping me during my studies!

[@JaseThePhoenix]

I wish my professor did the same thing to their lectures, would save me time reading textbooks

[@lucaslopez2091]

you should never replace reading the textbook, these lessons are complementary since they are simplified.

[@ekish9253]

True i agree

[@ghufran9635]

THAT WAS AMAZING!!! For a moment I imagined you teach us in our university, it would be like a dream 😢 Thank youuuu so much

[@mydude9710]

You can hear the passion he has for teaching science in his videos. Amazing job!

[@uhm9361]

i know right frfrf

[@jettburns8879]

That's amazing, to think we can do this inside cells using biochemistry to get what we want! Keep them coming Sal, loving these new (micro)bio vids.

[@youngspunky2835]

gucci mane is a clone go look at him he is a human so yea do your research XD

[@lucajones2349]

This is genetics, not microbiology.

[@AlunaScape]

Umm i think to be precise its biotechnology-biomolecular.

[@eniamrahc73]

Even in med school, you're still helping me. Thank you!

[@paribhura6704]

You are Doing this stuff in med school?

[@eniamrahc73]

@@paribhura6704 Lol unfortunately. We just started systems, but we covered this in our basic foundations 1st semester.

[@paribhura6704]

@@eniamrahc73 oh! We are doing a brief unit on it but I am in smiths grade, not many details

[@weklihua]

@@paribhura6704 just have to go back to this stuff again and again…..

[@rotanux]

Me too lol, first semester of med school, in 2 days I'll have my exam

[@gladysdiekonifeoluwa4691]

You just simplified the bulky and complicated material given by my lecturer.. Thank you so much ❤🎉

[@malakgalhoum9907]

thank you very much i wasnt understanding it until you explained

[@amandaz1454]

started watching khan academy when i was in 9th grade and hi i'm in my first week of college turns out no professor teaches better than Khan :)

[@0001-exe]

Bro SAME! i also started watching since 9th grade and am now in my first year in college

[@alperen3486]

Bob Ross of biology :D

[@samsalla2381]

The heat shock is happening through CaCl2, which is counteracting the electrostatic repulsion between plasmid DNA the bacterial cellular membrane ( liposaccharide layer), mostly E.Coli as it is most competent bacteriathan for these procedures.

[@sriramsidartan690]

Thankyou for the explanation

[@lillyherbel2124]

Thank you so much! I’m in biology D as a freshman right now, and with the whole online learning deal, I’m struggling in the class, and this made the whole idea wayyyy easier to understand! Thanks!

[@gregsmith6756]

You would not even be doing this level of work as a freshman. It will probably be the first semester of your junior year before you start conducting these kinds of experiments.

[@nitro.2405]

@@gregsmith6756 whats the point of responding 2 years later? lmao

[@GokulGopakumar-GG]

​@@nitro.2405for others whom it may concern 😊

[@RaviGarlapati]

Watched this video to understand what the term "DNA Cloning" means and how that's done with the help of recombinant DNA technology. Got to know that it's reproducing of the desirable genes/sections of DNA with the help of certain restriction enzymes, DNA ligase, and the host bacteria that supplies plasmids to attach the target genes and then reproduces them. This video also clarifies the question - "How to engineer microorganisms?" - for which I have seen other videos earlier (insulin production as a case in point)

[@giftlesibatleanedemenaceth1649]

Which biotechnology techniques can be used to proof if certain cell express a particular enzyme? I wrote Elisa and Western blot. Pls help?

[@letspractice9444]

That's the exact way of teaching....loved it

[@daisyrivera9417]

Thank you! I was reading my textbook and lecture notes, but still couldn’t understand it. This video explained everything perfectly 👌

[@meredithgrey5316]

WOOOOOOWWWW OMGG THANK YOOOUU I was watching all of my videos from uni and reading all of my lectures...didn't get a thing.....and you are here making it so easy, you literally save me from resit.

[@head4shot]

This is the most concise and informative video on this.

[@moskar2738]

Such a great video!! Explained this concept so clearly!

[@ScienceGhetto]

I really love khan because learning becomes easier.

[@elissar2004]

His videos are life saver!!!

[@life9162]

Who else reads the comments while sal is explaining 😂

[@salamlababidi2644]

Great video! Thank you, Sir. May I say that It's pretty amazing how you're fascinated by science 😂 you transferred that to us too!

[@pirate1367]

You made my reading texbooks life easier

[@rania_hashim]

SUPER helpful content - thank you SO much!

[@satishraina4434]

Excellent Presentation. Very well done. The colours (against the dark background) make the intended impact.

[@matteopradal5581]

Very well done!! best explanation of this topic

[@user-ys2ch1sd5i]

Thank you for helping me through my lab courses. I would be lost without your videos.

[@ManyHeavens42]

The first humans lived to be 900 years old now you see why its so important

[@flipperdeflipperkast]

thanks!!

[@gamestarz7030]

Thank u sooooooo much . U saved a lot

[@zienanema4691]

Thank you very much your are agood teacher

[@msms3737]

thank you didnt understand what the overhangs were and overall process of DNA cloning

[@-zl5go]

Pro you are amazing person..really you explain any object i want to study it ..i don’t know how i can thank you 😢❤❤❤

[@nikki49010]

You make everything so much easier to understand

[@shroqhafed5717]

Thank you 🙏

[@kluaywael]

Thank you for the explantion🌻🌻

[@carjanice]

could you make a video and explain different kind if cloning? Gateway, Greengate, Goldenste are some examples I can't differentiate from each other...

[@pavan4147]

I have a doubt sir. Like u will collect DNA fragment of a virus r plasmid of a higher organism then u will maintain it in such a organism within which u can easily insert a foreign DNa fragment Then which DNA acts as vector here N how many organisms DNA will be involved ..??

[@Lebrondagoat561]

Got me through my assignment, thanks sir

[@marija347]

How do we know for sure that the bacteria will express the genetic information from the plasmid, for example, even if the colony has taken up the plasmid, why would we be sure that antibiotic resistamce will be expressed?

[@Pepper-d1h]

나 한국인인데 쌤 설명 지리네요

[@jyothsna.m1053]

Thank you so much

[@JKSmith-qs2ii]

I was wondering the following, this video tells me that DNA is taken from one organism and put into another organism's plasmid so that that organism can go and create a desired protein. My question is then, does the bacteria know how to splice the introns and exons when it's creating the desired protein. Much more logical to me would be that they take ripe mRNA, (already spliced correctly) reverse transcript that and put it in the plasmid, since bacteria don't have introns and exons.

[@ayandamafunda312]

Isn't bacteria a prokaryote and prokaryotes don't have introns.

[@vickychen5984]

make it easy to understand!! and it is almost identical to my lab materials but in simplify form!! Thank you!

[@GM.Nobody]

the only good thing about youtube algorithm is that, it introduced me to Khan Academy

[@mariposacuriosa]

Wonderful explanation!

[@AmiraMohamed-xr8dg]

Great explanation. Thanks a lot.

[@gregorysmith1134]

Excellent lecturer. Great clarity.

[@chaimataifi8799]

Awesome explanation. Thanks a loooooooooooot.

[@smujb5544]

this is good

[@noufalsaedi9624]

You are the BEST!!!!!!!!!!!!!

[@Mafnu]

Thank u

[@aquahot]

Thank you Sal. I wish every teacher is as awesome as you are

[@athilalthaf6222]

how do we know that the bacterial colony with the desired product only have the antibiotic resistance?

[@sameervyas8375]

Because we are using plasmids which carry the factors coding for resistance against some antibiotics and the scientists who perform these experiments are well aware of the plasmids and the resistance they have ..and as the replication occurs in the E coli this gets amplified...

[@vijithanambuwasam8795]

Thank you very much .finally I got it.

[@stephenoclarke8703]

excellent tutor!

[@chloetaylor6789]

Thank you for getting me through my studies! These videos really help piece it altogether. Much appreciated🙌 Your Northern Irish student😁

[@bilalclasher4722]

Is it me or any one else found that Sir's voice is quite similar to Dr Morton ( the noted anatomist yt channel)

[@kierenrogers425]

Great vid Sal, you're the man!

[@EclipZeMuzik]

awesome video!!

[@suhyu_n217]

와,, 이해하기 쉽게 설명 진짜 잘해주심 ㅠ

[@rinaa5877]

Love the Star Wars reference

[@Wabuh-Wabuh]

What is the solution in the test tube made of?

[@mariapaz8739]

Thanks you have helped 😊

[@vincentdaly1766]

谢谢

[@vamp3]

Can someone answer me PLEASE!!! About how do we select the bacteria that took up the plasmid .. what I know that the Plasmid has antibiotic resistance gene NATURALLY (because he said we place it!), so obviously the other bacterias in the plate also have it, so they won’t die ?

[@danialabbas269]

the whole idea behind the plating and growth is to allow the bacteria with the resistance to grow and then bacteria that don't have the resistance won't grow colonies. Now that being said, it is science and sometimes the other bacteria will also grow due to naturally gaining the resistance, but that doesn't mean their numbers will be profound.

[@30vansan]

Superb!! Very nice, simple explanation.

[@saiwashburn]

Love the vids. Can you record at just a slightly higher volume please?

[@ssgcharm12]

I can't sure that you respond to me, but I have a question. Is there any checking process that we can check the plasmid has a gene that we want to clone between 'Paste process' and 'Inserting the plasmid to the e.coli' ?

[@aroojqureshi317]

Nope..no way

[@syedmianzalaniqbalkakakhel7091]

Well explained lecture Sir!!!!!!

[@saurabhgogisetti]

i thought Restriction Enzymes cut the Plasmid at Restriction Sites

[@Paranoidhuman]

Pretty cool but you didn't actually explain what is a recombinant. Or maybe you did but didn't mentioned it explicitly.

[@mahendrahosalli4803]

Tq so much for your kind information

[@zoom-zip3473]

How do we compensate for the lost DNA fragment that was cut off for cloning?

[@mydream3914]

Is there any videos about methods used to obtain DNA sequence?

[@barrylitser3793]

great video, thanks. But i have a question: How can we know that the plasmid does contain the sequence of interest and not another sequence that happens to use the same restriction enzyme?

[@sahildasaur2072]

you can typically run a gel electrophoresis of ur isolated gene of interest next to a gene ladder that will show the expected bands of that particular gene of interest. If the two sets of bands correspond with each other, you know you have your correct gene of interest!

[@MrWaleroTV]

what are the products of DNA cloning?

[@mohammedal-hammadi5085]

You are awesome really, very clear and good video, thank you so much, we will wait for more videos

[@lucindadewaal9511]

Thank you thank you thank you

[@hollystoltzfus758]

Which video describes the extraction of desired product? Insulin, for instance?

[@elenanewton5056]

Holly Stoltzfus

[@daniellewebb9921]

You would just add a restriction digest that flanks either side of the gene of interest.

[@CHBSAfadeelumarkhan]

Awesome

[@Protomaximus]

Homie mentioned the Clone Wars haha. They really know their audience

[@sadaftagar220]

hello can u plx tell me from can i get DH5 Alpha e.coli? Do u have any link from where can i order it

[@NusratJahan-iz5rc]

Thank you Khan academy ❤️

[@thomasdeconinck1492]

I am currently making a integrated test and man, you helped me a lot. Explanation is so good :)

[@LePrincee]

We love you Sal❤️💪🏾👑

[@puekai]

The drawing is great!

[@ELPaso1990TX]

So is it possible to clone p53 tumour suppressor gene and use the p53 protein to treat cancer?

[@ashish.cenation]

closer but still not able to explain completely....how will we all of sudden will introduce antibiotic resistance gene in vector...won't it too will take process of colony formation selection of transformant same as that of our gene of interest...so by your video concept before clearing give rise to another doubt

[@boooo468]

How do you know if the plasmids taken up by the bacteria also contained the gene of interest? Couldn't some of the bacteria take up plasmids that gave them antibiotic resistance but did not include the gene of interest?

[@notonfire7318]

Because all plastids contained both antibiotic resistance and the gene. So either both went in or none

[@notonfire7318]

But I'm not sure I'm just guessing

[@aroojqureshi317]

We use blue white screening for this. The transformed colonies are white ones.

[@nourm252]

Man, thanks I love you.

[@WithFaizanKhan]

What Software Is Being Used In This Presentation ?

[@jaykwon7472]

idk

[@tunneltrance]

they'll never tell you, they keep it a secret

[@tunneltrance]

I would bet its an ipad app that they use. And they hook a mic up to it and draw w/ a stylus

[@nomnomisstillhere8542]

it's secretly ms paint but with a black background

[@feskii]

In case you don't know even though I'm late it's smoothdraw

[@mynguyenthitra567]

While ago i can watch this video in vietnamese about cc, i can’t watch other now:(((

[@academictraveling]

I'm 22. But can GOM work on whatever ages we are? or it only works for the embryo? haha.. as an example in my age 22, can I get blue eyes?

[@WaiKeat66498]

Thank you. It is a very nice explain!