Leaf Color Chromatography - Bite Sci-zed

[@amberwalker8956]

I promise you are a life saver!!!! I take Biology online and I'm not your typical science major. This video is a great breakdown of the process for my next lab. Thanks a bunch!!

[@AlexDainisPhD]

+Amber Walker So glad I could help :)

[@reemgomaa3627]

or should u say leaf saver..

[@irejgerard6599]

This video is amazing! I used Momordica charantia (bitter melon), Moringa oleifera (horseradish), and Psidium guajava (guava) and was astounded with the result of my experiment in paper chromatography. I watched your video and followed it closely and it helped me a lot throughout the process. With the help of your video, I witnessed how great science is when understood. I am still amazed at the partition where the solvent seeps along the paper carrying the substances from the mixture to the one end of the filter and the pigments were separated; the result was even impressive after it dried. Students like me are truly grateful for your amazing video. Thank you, Dr Dainis, I am looking forward to learning more about Science with you!

[@mrericsully]

A suggestion for keeping the filter paper from touching the side is to lay a pen or pencil across the top of the bowl/glass and then tape the filter paper perpendicularly down from there. It has worked well for me in the past with ink chromatography.

[@thingatbui2526]

you're wrong you idiot

[@davidpeters4334]

Who is watching this for science class?

[@mingmatamang5381]

David Peters I am also watching for my science class

[@ethanp4050]

wow cool man

[@bhavnasingh7259]

David Peters meh ....😊😁

[@seancena2598]

you suck.

[@seancena2598]

big shlong.

[@steelshade]

Alex, you have great enunciation. And just the right amount of movement, neither still as a statue nor spazzy. Knowing that your already good video skills will only get better earned you a subscription.

[@AlexDainisPhD]

Chlorophyll is more polar than the carotenoids, so it sticks to the paper a bit more. This means the carotenoids are drawn up by the liquid faster and appear above the chlorophyll.

[@nikkanarciso1365]

I have used the common leaves found near my house, it includes: Water Spinach (Kangkong), Oregano, and Malabar Spinach (Alugbatti). With the help of the video of yours, I followed the steps of this method, Chromatography, and I had observed that the molecules have different polarities interacted with the coffee filters differently, layer by layer.Thank you for sharing your knowledge, Dr. Dainis, I enjoyed doing it so!

[@cristelannedionisio3092]

I used oregano, chinese evergreens, and devils ivy. And the result was great the color of the isoprophyl inside the glass with some leaf really changed, and the paper changed color a bit but it was really amazing. Thanks for the info Doc. I've learned a lot.💙

[@porgy29]

If the leaf is colored, it has pigments and they can be separated. Now if the leaves are all brown the pigments have probably broken down already, but if you live in an area where the leaves don't change color, they still have pigments and can still be separated.

[@azermanus]

You are awesome. Good to see that I'm not the only dork who finds all this stuff fascinating. Subscribed.

[@MajidTheComidian]

OMG you saved my life, without this video my project would have been a straight 0.

[@DANGJOS]

You're right Alex. Xanthophylls are part of the carotenoid group. Leaves normally contain both xanthopylls and carotenes and as for which one dominates depends on the type of plant. Spinach leaves for example are famous for their high lutein (a xanthophyll) content because they have significantly more lutein than beta-carotene.

[@mikesmith410]

Omg.. That was magic, one minute she didn't have her glasses on and the next minute the glasses reappeared, I'm impressed..

[@657tgjhf]

Strictly speaking this is not paper chromatography. The solution in the beaker is just the solvent, not the mobile phase of chromatography. The trick is to choose a mobile phase which separates the different components of the mixture.

[@ChristianoBorst]

Target alcohol! Up and Up products go on sale across the board during the fall months.

[@Reivivus]

This is because the isopropyl alcohol pulls the water out of the leaves through the porous membranes of the leaves by osmosis, right? And as the water is drawn out of the leaves it pulls the compounds with it, and the compounds like chlorophyll and carotenoids are drawn up the filter paper by diffusive capillary force, right?

[@AlexDainisPhD]

It's actually just "I Love You" in American Sign Language. Just trying to spread some love!

[@zachboi13]

"the leaves are being seperated from alcohol phase, the mobile phase and Drawn up into the paper, which is the stationary phase." Is this where we get the name"stationary" for paper and paper supplies?

[@paulogeronimo8575]

I used Oregano, parsley, and mint. As I observed it separate substances based on their color as was the case with herbal pigments.

[@zanzibarland1]

Hey! New subscriber, medicinal chemist from Australia. This is probably getting old to you now but you are amazing! Keep it up!

[@alexanderflieger3649]

I don't mind losing to science. But yes I find your videos awesome. Can't wait to watch future ones.

[@ebsolomarydelnique6743]

OMG THIS IS SUCH A LIFESAVER AND VERY EASY TO UNDERSTAND THANKS!

[@AlexDainisPhD]

Thanks! I was so excited to be in that video with so many awesome, amazing KZbinrs! Hope you stick around and learn something :)

[@DANGJOS]

Some leaves will actually turn purple to nearly black in the fall because the anthocyanins are produced before the chlorophyll degrades

[@AlexDainisPhD]

Yup, different trees will have different pigments in different amounts - this is what gives them each their unique color! For these kinds of pigments, I'm not sure about if you can influence them (my guess would be yes), but I know you can in many flowers. For example hydrangeas will produce more blue blooms if planted in acidic soil and pinker blooms if planted in more neutral soil.

[@dawitworkuhasen7816]

Your are a life saver for my science assignment 😊😊

[@MC921109]

Idea for another video? Anthocyanins are not actually always red(I know you know), its colours depends on pH of surrounding. Another pretty experiments can be done with them. You can use chromatography to isolate them and then make solutions with different pH, few drops of anthocyanine solution and magic!

[@AlexDainisPhD]

Yeah, it gets a little echoey at times... I'll work on it!

[@Nikusha-ny4xc]

OOO LOZER

[@koitheawesome]

I got here from the numberphiles video. I'm definitely going to subscribe. I love videos that teach you things. And plus, your smile is so very charming.

[@ivanwangsaputra8506]

Hi, I'm from Indonesia, glad to see that you are full of enthusiasm in providing the knowledge you have, sis, may I ask, how to treat living plants so that their color changes from green to partially white, and permanently, what should I do, thank you sis for the time . GBU

[@AlexDainisPhD]

Thanks! I keep trying to make each one better than the last, so I hope you continue to enjoy them!

[@AlexDainisPhD]

Hah, it's definitely not - most Bostonians drop their R's and turn O's into long A's. Who knows where my crazy voice came from. And thanks for watching!

[@MC921109]

Yeah, we´ve made that experiment very recently. Tip for cooking enthusiasts : try to add some sodium hydroxide to your red cabbage next you are cooking(no, don´t actually do that...) What are you studying, biochemistry?mol. biology? Super-duper videos btw :) and sorry for eng, i´am trying...

[@TheDylanBowman]

I'm stoked that NurdRage sent me in the direction of this channel. I love me some science videos!

[@zeiadgber7807]

what can I use instead of isopropyl alcohol ?

[@koitheawesome]

I have in fact been keeping up with your videos, and they have all been very interesting. Your presentation is awesome, and you pick some really good topics. I even go around and tell all of my friends about some of the stuff in your videos. You have so much interesting information.

[@megatdesigner6635]

great video!!!! i wonder what will happened if i change isopropyl alcohol with hand sanitizer.

[@GrimTheCrow]

Thanks for showing us this channel.

[@Kritisaxenavlogs]

Very nice experiment of science l know many things about the leaves

[@johnoliversantiago2476]

Can you do a Bit Scized on the effects of alcohol on the body? And how exactly does alcohol poisoning work?

[@GrimTheCrow]

For some reason I started to think of Baphomet when you mentioned the story of Pan and his horn(s?). Look up a picture of Baphomet, I think it is closely related to what you talked about.

[@profspop]

I cannot believe how much content you got into 4 minutes and 24 seconds. Does the mass of a video increase if you put more content into it?

[@krazyking424]

Awesome experiment instructional! Also more informative than nottinghamscience's "Why do leaves change colour?" from the other day. I'll try the experiment just as soon as there aren't 60mph winds outside.

[@SgtMantis]

They certainly do, but I just thought that they were so beautiful that they should not go without honorable mention.

[@smithafrancis5877]

I have to do some assignments from home ( because of Corona ) you are a lifesaver!!

[@AlexDainisPhD]

I love epigenetics (It's what I want to study in grad school) but there's so much and I think it's so important that I want to make sure I do it right. I will definitely make a video (or two, or four...) on it, I just don't know when/how yet!

[@cameronbevan3454]

and also can any alchole be used for this experiment or is it only the type you stated?

[@pheakdeynorphear2656]

Hello,What is the advantages of doing this?Because i need to answer with the asker when i do it.

[@JamesShrefler]

OMGGGGGGG this was so good my class liked it

[@isaiahteruel2014]

is this a photosynthesis expiriment?

[@Xavierlindseyy]

Do you know what we are measuring in the project

[@giga.s.o.p]

Great video! I remember we did chromatography with blue ink in school, the result was some colorful paintings in the paper :P I will try to do this at home :)

[@mmkmaram]

Wow, I admire you!! I have a question I'd like to ask. I'm doing a science fair project based on this experiment so it's the beginning of January already. Luckily, I have got some leaves from before hand but I only have two colors of the same bush and another color from another bush. Should I just use the leaves from the same bush or use the extra leaves from the other bush too? And would you mind giving me any advice for my project? Thank you, and greetings from California! (PS I have watched this vid about 5 times)

[@AlexDainisPhD]

I think it would be interesting to try all of the leaves! That way you could compare both the same bush at different times and a different kind of plant. The more kinds of leaves you use, the more interesting things you might find!

[@pepilindo]

we are starting spring, so we may not get many different results from the leaves

[@lavieh3drelaxmelody773]

Good little teacher, like that style of course

[@Stoereboi13]

Is there a way to the separate the alcohol and the chlorophyll so it would leave a powder or paste?

[@user-rx6fb6tf8d]

Why can’t the filter paper touch the glass?

[@NisseHult101]

Nice, that's a really cool experiment!

[@iloveuverymuch5178]

i wanna see what happens if i use blood to water my plants and then do a chromatography on em. they would probably die right?

[@carmitsci]

Great initiative, its nice to see someone who simplifies experiments so that kids can try these at home. It would be even better if you slowed down your talking a bit, its hard to follow with your hand movements and fast talking. If you had less liquid in the cup the separation would have been easier.

[@whine0with0wine]

Most cooking/chef stores will have a mortar/pestle .

[@reniegin79]

What type of leaves did you use?

[@falconseye97]

i want to do that in grad school too! What's a good one for it? I'm looking at MIT but I might need to look elsewhere.

[@zhubajie6940]

Maybe you can talk about electrophoreisis since I think this is used for DNA (though I don't know how the DNA is died, colored I mean) On youtube search for "Emily's home gel electrophoresis experiment"

[@parampreetkaur3475]

Vry nice video ..... Helped me a lot one day bfore my experiment ....

[@chicklette100]

Gosh I started watching UR vids at 9 and I looked at the clock and know its 10.

[@dayiie427]

Question: I'm doing this for a science fair project but how am I supposed to arrange my data?

[@shehryarmanzar725]

Made on my B day!!! I love how you explain all this science in a fun way!

[@niveditadas3540]

Can we use normal alcohol instead of this

[@GMCLabs]

I did that with weed once, but I was looking to isolate different compounds.

[@itsjccccc]

I use oregano , makahiya and lagundi leaf in this experiment 🥰

[@Nobarker]

Hi I found this link from NurdRage, Like what you are doing here. P.S. I like the glasses quick change/continuity thing.

[@sabrinalin9111]

what is the purpose of this experiment?

[@kentvandervelden]

Your kitchen setup was borrowed in v=ej2zXOwASVI.

[@AlexDainisPhD]

Huh. And they put their own watermark on top of it. How annoying... (But thanks for the heads up!)

[@zynnyzoo]

Thanks for the info; my science teacher assigned us a project on pigments and the like. Again, thanks much.

[@chocken4867]

why do you use alcohol instead of water? Please answer this my lab is due tomorrow LOL

[@theatticmonster1110]

Craaaazy idea, would the trees turn their leaves red before they fall off to help them decompose maybe? Then the tree can re-absorb them or what ever? Tree cannibalism!

[@khm2128]

I think the sugars would help feed the mycelium in the soil. So, yes. Your idea sounds feasible to me (I'm just someone from the internet, tho.)

[@partingofways]

@Alex Dainis Did it blind her?

[@Osmancique]

I like your stuff very much but I have a suggestion. Cover your walls and floor with something. Get a carpet, pile some bed sheets, pillows or luggage on the walls. The sound quality isn't very pleasant.

[@AlexDainisPhD]

I owe him a bag full of nickels, I feel so honored to be in that video with so many cool people! I'm glad you like it!

[@aryastarkisfuckingqueen8942]

how much alcohol did you use?

[@blue7maggie]

my science teacher sent me this link, and we did the experiment in class

[@bigedman365]

I always wonder how people are doing several years after their comment. How have you been? Are you still in school? Do you have a job? Are you still alive?

[@jimdanial5735]

If i'm not mistaken, Vsauce have a video on why the leaves turn red...

[@jimdanial5735]

the video is entitled "Mistake"

[@jimdanial5735]

Sofea Ghani watch this!

[@SoullessScythe]

@Michael Lewis You are far from alone i Taught myself how to Program at age 13

[@Blackline60]

We all want a Featuring of Nurd Rage and Alex Dainis ! The Couple scientist ! Ahah ^^

[@귀여운띵둥]

hi. i am a korean student. i was impressed by your experiment. i want to capture this video and use it in science club. and i want to put the course in the club cafe by using this picture. i will reveal the source. can i use your video?

[@ogluster8873]

Your accent is so unique!

[@zushiji1039]

but what if im doing on how they get old

[@sultan__8636]

i just want to search how to make a leave from natural to artificial without changing its color

[@2601denise]

Harry potter books in the background , :D

[@proexytgaming6618]

Where you live

[@wilde7061]

i did it in shoo with some norma leaves and there were green, yellow and purple

[@khm2128]

Did you mean shoe or "schoo" ?

[@sinekonata]

1:42 that is one awesome theory, I love it :D

[@brooklynbrowders1487]

People play more attention to the glasses than the experiment

[@SgtMantis]

Xanthophylls?

[@stanleycostello6541]

Wow. Pretty cool, I must say. Another subscriber, count me in!

[@NaolynInagnao]

thanks for movie helps alot in my study, really interesting...

[@AlexDainisPhD]

I believe xanthophylls fall under the carotenoids. I didn't want to get into too many of the types of pigments (chlorophyll a and b, etc) but yes, there were probably xanthophylls in there!

[@ggvddfhgc9462]

the video image is too poor, you need to fix it more

[@cameronbevan3454]

great video! i am going to do it for my science fair with my friend! i recommend you do a video on sodium alginate balls in photosynthesis, it is a very interesting experiment and that will get many subscribers!