Method development by HPLC

How to select appropriate chromatographic conditions
💻Live Workshop: HPLC Method Development by AQbD Approach💻
⏰Date & Time: Sunday, 19th Jun 2022, from 10:00 am to 2:00 pm (IST)⏰
Free Webinar Registration link: us02web.zoom.us/webinar/regis...
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[@anurimadatta4401]

Sir can you please arrange one more session on method development of Hplc.....or can you please avail the recorded session which already occurred...

[@siddhachavan8604]

Excellent video made.....

[@sekhardammalapati931]

Excellent video sir

[@bestdancevideos2253]

Iam Big Fan Of You Sir

[@b.k4044]

Sir Please provide the recorded video of the seminar !!!!!

[@londonvlogs7246]

Hi sir Could you please arrange one more session on HPLC method development

[@srinivasaraosadagana5233]

Excellent air

[@surjitdeka7092]

Sir. If I send a sample for quantification of compound and also for method development to a institutions. should I give methods ? Or they will do by themselves

[@srinivasaraosadagana5233]

Excellent sir

[@santoshbhujbal5977]

Really helpful

[@Aaakashkaushik]

Sir please make a video on potentiometer (auto titrator)

[@srinivasaraoezzada6677]

Sir I missed the interaction session pls pls upload that Vedio, i am very enthusiastic about that pls sir

[@surbhisingh9824]

Sir please add your webinar video in your you tube channel

[@onissi4562]

Sir method development full video please link in KZbin sir

[@bestdancevideos2253]

Super Sir

[@bobbalisandhya4861]

Sir please make a vedio on hplc impurity data analysis

[@talluriramesh372]

Pls give about message in HPLC columns

[@muralikrishnareddykoppula2210]

Sir plz gradient phogram create calculation

[@shrutidixit499]

Pls add a video on ion exchange chromatography and with monoclonal antibodies

[@bestdancevideos2253]

Please Upload How To Select the Column In HPLC and GC And Mobile Phase Please Upload Sir

[@labhanabikesh4290]

Sir please upload 19 June 2022 webinar sir please helpful to me more

[@adnanazhar7227]

👍👍👍👍

[@PraveenKumar-mg6xu]

LC-MS development please

[@maheshgupta564]

What is difference between uv spectrophotometer and HPLC

[@pharmagrowthhub3083]

UV spectrophotometry and High-Performance Liquid Chromatography (HPLC) are both analytical techniques used in chemistry, but they serve different purposes and operate on different principles: UV Spectrophotometry: Principle: This technique is based on the absorption of ultraviolet (UV) light by molecules. When light passes through a sample, certain wavelengths are absorbed depending on the molecular structure of the compound. The amount of light absorbed at specific wavelengths can be used to identify and quantify substances. Usage: UV spectrophotometry is widely used for quantifying the concentration of a substance in a solution. It is often used in biochemistry and molecular biology for the analysis of nucleic acids, proteins, and other biological compounds. Advantages: It is a relatively simple and quick method for analyzing samples that absorb UV light. It requires small sample volumes and is non-destructive. High-Performance Liquid Chromatography (HPLC): Principle: HPLC is a type of chromatographic technique. It involves passing a liquid sample through a column filled with a solid adsorbent material under high pressure. Different components of the sample interact differently with the adsorbent material, causing them to separate as they flow through the column. Usage: HPLC is used for separating, identifying, and quantifying each component in a mixture. It is highly versatile and can analyze a wide range of compounds, including those that do not absorb UV light. Advantages: HPLC provides high resolution and sensitivity. It can handle complex mixtures and is capable of providing detailed information about the components of a sample. In summary, while UV spectrophotometry is primarily used for quantifying substances that absorb UV light, HPLC is a more versatile technique that can separate, identify, and quantify various components in a mixture, regardless of their UV absorption properties. The choice between the two methods depends on the nature of the sample and the specific analytical requirements.

[@maheshgupta564]

Thanks sir for easily explain

[@maheshgupta564]

One interview ques asked that what is reason of molecule absorption

[@maheshgupta564]

Sir can u explain what is %cv in dissolution calibration

[@sureshqc9006]

Hi sir

[@yoginagam533]

Hii sir

[@gauravprajapati3915]

Hi

[@moviedownloadpurposes458]

Hindi please sir

[@nagamaniram4296]

Super sir

[@analyticaldiscussion6789]

Hi