I put my DNA under the microscope and you can too 🔬 Easy DNA extraction

[@RoadRunnerMeep]

This is the type of Science I wanted to learn in school

[@prasadborse2161]

You have to take genetic engineering as a career

[@deputydan2226]

Me too, now im grade 10 we are learning about gender 127

[@devdootmondal7115]

Exactly you are right I am in class 11 now

[@Krack2805]

​@Scott Pickering how is this "basic" science? because he made it seem easy using common materials?

[@Krack2805]

​@Scott Pickering ​ @Scott Pickering in what reality? hopefully the one where actual scientific thinking applies what "basic science" do you figure out what is or is not dna in a pool of stuff? how do you know what breaks down proteins and cells to get at dna? how do you know how to separate dna from other things? can you explain the "basic" science to get at all of the information being presented for each step? So, since its "common materials" it's "basic"? regardless of how many non-"basic" sciences use common materials in a lot of their processes. "its looking at stuff through a microscope".... this is like saying "its looking at stuff using a calculator/computer" for rocket science.... If you just "looked at stuff through a microscope", would you know what you are looking at at all? Keep in mind how he can't verify that these things are indeed dna or not, or if the clumping is from protein or not; he's making educated guesses based on "basic" science? Do you think "astrophysics or rocket science" can't have small inaccurate experiments like this that make things seem "basic" because it's not a "basic science"? How exactly do you define "basic" science, because I don't see a consistent approach being used here.

[@willmcconnell6008]

It's likely an aggregate of supercoiled DNA, the 300nm loops I think would still be way too small. And if you recently ate something especially with starches, you might be seeing a lot of alpha-amylase proteins! We did some similar things in my Biotechnology lab. I think this a great activity btw, it's a really good basic example of DNA extraction without needing purification columns and lab grade detergents

[@SystemUpdate310]

It could be condensed chromatin fibers, which is roughly 700 nm wide. That could be seen with a light microscope I think.

[@tannukashyap8311]

why do we see alpha-amylase proteins after eating starch?

[@willmcconnell6008]

@@tannukashyap8311 Looking back on this you wouldn't be able to actually see the proteins like that, too small. I think I was assuming there is an increase in alpha amylase enzyme production in your mouth during starch consumption to help increase digestion, but I don't actually know if that's true

[@willmcconnell6008]

@@SystemUpdate310 I think it's possible to see 700nm in a light, and yes it is condensed chromatin fibers, heterochromatin is 700

[@jackiegonzalez5789]

@@tannukashyap8311 they are enzymes used in the digestion of starch So if you eat a lot of starchy food, then your cells (Intestinal for this one in particular) would respond by synthesizing more alpha amylase enzymes To help in the digestion of it

[@melanoficus]

I am sure you can do fluorescence microscopy . You could use a DNA fluorochrome stain such as Acridine Orange to stain the DNA. Alternatives are natural DNA staining fluorochromes include Berberine (from Barberry - Berberis spp.), and extracts from Greater Celandine (Chelidonium majus) which is a common plant in many parts of the world, and Plume Poppy (German = Federmohn) (Macleaya sp). You don't specifically need UV for this fluorescence, because most of these stains can be excited with Blue light and emit a yellow gold color.

[@ilyryoga]

Amazing!!! I wish more people got interesested in content like this!!!!! Just fascinating, thank you very much

[@oparaprince4586]

Totally magnificent dear

[@heywhatsup2359]

Exactly :)) people should show their more interest in science ...

[@damontsekos365]

Najma Samantha...Microscopy, and amateur astronomy are the best hobbies anyone can get into!..It sure beats coin, and stamp collecting which I find to be dull, and boring!

[@amonman1538]

@@damontsekos365 hobbies are subjective. I find stamp and coin collecting boring as well but you can’t go around telling people what they should be more interested in

[@Feeeeeeeeeeeesh_10]

People are suprised that I’m 11 and I like science and not stupid video games

[@saram2446]

what kind of microscope is neccessary to really see the DNA?

[@johnharvey4496]

Must admit the title and thumbmail got me it - "look at my DNA under the microscope" and the head of Microbehunter - I'm thinking, wrong website/hub. But no, it's educational, what a surprise.

[@mra7860]

I am a young and new passionate science teacher! Glad I found your channel! I love learning the content you produce Sir

[@pranavlahoti]

Sir you could use lens cleaner as protease to degrade proteins (histone) that binds up dna and see in micro scope of high resolution you may get a better view of dna

[@apoorvakumari6410]

Such a genuine content . Not the cringe as other creator I wish this kind of proffesor are also present in pur college🌸🌸

[@sudhan152]

Hai Apoorva were is our college in India

[@marcdemell5976]

Academia has an agenda ,,,keep them dumb ,,,,not too dumb.Evil to the lying core.

[@ishitvvats2044]

you should've used eye drops as they have cellulase and amylase and starch would be destroyed, more chances of actually seeing DNA. although i think the micrographs you got at the end, the little beads on string are actually DNA. you madman Oliver I love you you did it

[@ivan_romi]

Im learning a lot more with your videos than with my classes at the university. I've seen your gallery, it's fantastic, I use the pictures to compare what Im looking in the microscope. Your job is amazing

[@Krack2805]

point of classes at university is to give you the background to allow you to get to these types of things on your own, designing and figuring things out. just watching and learning specific things is mostly only beneficial for a particular job or task. it can be more motivating because its more interesting, but it should motivate you to learn the class stuff.

[@SolidSiren]

Then you must not be paying attention in your university classes...

[@UNDERST0RY]

The wood also good in case any of the DNA happens to be vampiric.

[@Microbehunter]

Sign up for the Newsletter: www.microbehunter.com/newsletter-signup/ Followup Video - Better DNA extraction: kzbin.info/www/bejne/aaLZnmatZ9-qnaM

[@anindyabhattacharya8793]

It has a beads on string structure so probably is chromatin material.

[@dennismoya4311]

yeah i agree

[@prasadguruju9322]

I had experienced and did many experiments in the high equipped lab while doing my post graduation in microbiology discipline... ! My researches included microbiology, biotechnology, biochemistry,cell biology and molecular biology... !

[@mikevanderman2727]

So you would say it is DNA that we are seeing?

[@shrik393]

I study b SC biotechnology 1st year. In my practical we do Isolation of DNA from both plant and animal tissue. it's gave a best results.

[@elikopokopo6443]

The open source licensing s very honorable. Thank you very much!

[@dgates6165]

Absolutely!! 100%

[@arnabisarkar1]

Sir in a very beautiful way you have shown DNA. It is really fascinating.

[@luyenpham7475]

DNA reacts with diphenylamine based on the conversion of deoxyribose sugar in the DNA molecule (not in RNA), so when hydrolyzed at high temperature in an acidic environment to form hydroxylevulinyl aldehyde which reacts with diphenylamine to form a blue complex sustainable construction. So you can do this extra step to identify colored DNA when observed under a microscope and see :)

[@macb.43]

Perhaps a silly question. Shouldn't the utensils and equipment be sterile?

[@KnuNetzer]

How do you know it is DNA and not bits of salt, washing up liquid, old food bits, saliva or water etc?

[@niramaygogate7904]

Hey, it's been about three months since you posted this video. Any updates? did you confirm it was DNA?

[@cleowilson6502]

Good question

[@МиленаКамчева]

It is DNA

[@VV-yh3ni]

@@МиленаКамчева Лаборантка? :)

[@yeetogami2575]

Can you please give me the measurements of all the things that you used? I tried it but nothing happened so I think my measurements were off.

[@yeetogami2575]

I'm trying to do a project on this...your support would be very helpful!

[@Microbehunter]

I did not measure anything, i estimated. Spoon of salt in 100ml water, drop of liquid soap, 96% ethanol.

[@yeetogami2575]

@@Microbehunter ok thanks so much!

[@damontsekos365]

I tried it and it worked!...Thanks!

[@ferterrazas1156]

Hello, I’m currently a freshman starting veterinary medicine and I’ve been looking for information on how “far” a microscope can let us observe. My question is, and I would appreciate if you help me answer it, could one observe the DNA of an animal thru the microscope? Other than bacteria and cells, what else can a microscope let us observe? Please and thank you.

[@TheRockMorton]

Most excellent spit sample microscopy analysis. Whether or not we could see dna is compensated by impressive discussion of observed micro forms and structures. Bravo!

[@Unknown-bt2yr]

Man, it's awesome. Keep up the good work 👍🏻

[@shabbirhassan2653]

How it possible to see a small molecule to under the compound microscope because the resolution is 0.3 micrometer and magnification power is 1500x while the size of DNA molecules is 0.6 manometer.

[@roslinplays2616]

Can you make a video of mushroom cells under a microscope?

[@toedancer1148]

I just downloaded a PDF on doing the banana activity. Going to do it with my grandchildren. It was great to see how all was done, Thanks

[@hitmusicworldwide]

Dishwashing solution. A non ironic surficant dissolves away the fatty acid in the cell membrane and lets the contents spill out. Great for disinfecting also

[@GioVanniDaThird]

wow i was just asking myself where would i be able to find library of these mico friends .Thanks alot!

[@tanvirbinlokman8576]

me too mate.

[@user-ct4dm4tf4y]

First of all how do u know this steps i mean did u learn it somewhere or read it in some text book ? How can i make sure that what u did now is real science ? Thank u

[@ARCTURUSRA]

You say looking at lood has no diagnostic value ! How is it that you see sichel cell ? Or how is it when you see an infection . Or let me go further how can you see if blood is infected ?

[@Microbehunter]

To check for infections, you do a PCR or an antibody test. There are only very few infectious diseases that can be diagnosed with microscopy. Malaria being one of them. Microscopy is simply not specific enough. If you have an infection in a wound, then you can of course isolate and see the bacteria, but then you already know that you have an infection....

[@TRANNGUYEN-nx5mx]

What is magnification Sir ? Thank you

[@grantacademy4300]

I know that the DNA, which is an acronym for De-oxy-ribo-Nucleic-Acid, is a double helix structure with some tiny steps and phosphate sugars on it but, I don't think I can actually see those things under my personal microscope. What is the name of the detergent you made use of? Is it Persil, Tide or Gain?? Or did you make use of any common detergent? I'll try this experiment once again and make sure I get the right viewing under the microscope. BTW, this video was amazing! Thanks for sharing.🎆🎆😁😁😊

[@pimpmyride7278]

I’m a cosmetology teacher and I have thoroughly enjoyed this. I want to find a microscope with proper lenses and magnification to view hair at its cellular level. I’d also like to be able to view things like hair color’s molecular structure. Any ideas on which strengths I may need?

[@Microbehunter]

There are many options. You might want to have a look at my other channel for help: www.youtube.com/@microbehuntermicroscopy

[@Soundwrecker]

Good one! Great topic. What kind of microscope are you fundraising for? I want to read the specs. :)

[@Microbehunter]

It's an Olympus BX53 with Planfluorite objectives (4x 10x 20x 40x 100x oil) and Differential Interference Contrast (DIC), which is expensive but produces very nice images. You need a separate prism for each objective and they need to be fit in into a condenser with a rotating turret. I obtained several offers, all similar in the price range, some even more expensive.

[@danielle7760]

@@Microbehunter OoO an Olympus! I hope to be able to donate too!

[@brentwejrowski]

Please explain how DNA is read. “A machine does it” never made sense to me!

[@mohamedqasem]

How much does the microscope cost and do you have a link for its website?

[@kellyklaask7su990]

I don't have my microscope yet. It will be here Wednesday. But I still find y our videos fascinating. I had no idea one could isolate DNA like this. I guess I better start saving up for my very own Scanning Electron Microscope. :)

[@AbsoluteAbsurd]

The laugh after “wood is good” XD i actually died

[@Duriel1000]

can you see the sars2 pathogen?

[@eodia1027]

You are an amazing person, This is my first video of your

[@mementovivere2]

Thank you for creating this image database!

[@dejanmalcic9349]

You have the best channel on microscopy, but you need more videos about vintage microscopes, vintage (and modern) staining methods, and diy staining (there's something on the internet, but I only believe in your recipes 😉).

[@Mr.Predator-2007]

Is it in compound microscope???

[@LeKikoojap]

i've never done more than school microscopy, but i wondered if just putting like an piece of plant or insect on a glass was enough to see inside and since you said something with dish soap, that breaks the structure and makes the cells inside visible, is this the way to make the inside visible? or is the inside visible anyway? i've read thing about microscopic knife too...

[@FawDot]

woah. That’s cool. Can’t wait for more content!

[@johnhanna1840]

I love your good vibes. TY for the teachings. Much love.

[@atharva-upclosingnature2423]

O my God! that was such an amazing experiment oliver.....Thank u so much..

[@prakashbiswas9148]

Wow excellently cool experiment

[@JonEllis-mi4mc]

Would be good to see magnification rate on website

[@jewel2647]

Wow how smart... Is the rounds things cells

[@vloggerboyshekhar]

And how much it costs?

[@NanaKwame96]

Incredible video! Seeing any amount of DNA under a light microscope of all things is far better than nothing at all. Thank you for the tip. However, can you go more into detail about this procedure? Never heard of this before and not too sure about the materials, for example if it is better to use 99% isopropyl alcohol or something much lower like 70%. Many Thanks again!

[@Microbehunter]

I have used 96% (about) ethyl alcohol. It works better when the alcohol is ice cold, but I did not cool it and it also worked. The DNA participation with alcohol is standard procedure in molecular biology. The alcohol reduces the polarity of the water to the extent that DNA can not be dissolved anymore. Normally one would collect the DNA by centrifugation after the addition of alcohol.

[@par-th1]

Bro it's awesome and you are a good content creator

[@ryuk.fr5]

Which microscope🔬🔬 you use to se dna normal or elecron microscope

[@Microbehunter]

Watch this: kzbin.info/www/bejne/rZqbnKOppN2qeKc

[@ykpoff]

Sir can we use sanitizer instead of Isopropyl alcohol

[@woodworkerroyer8497]

Not only is the video cool, but the micrograph website is really cool! Could this be done with a cheap educational microscope? Like a normal 1000x compound scope? Keep in mind I know NOTHING about chemistry and little about DNA, but could this be mucus or something? It certainly acts like it, but I dont know.

[@Microbehunter]

The microscope must be good and I also used phase contrast. If the microscope has a proper condenser, then maybe yes.

[@kusumpant3174]

The sound when he spit out salty water was satisfying 🙏👍👍😂😂

[@Lajos_Brekovszky]

Where did you get that test tube?

[@KS-sd3ih]

I wonder if you can look at them whilst they are in liquid...

[@yashaggarwal6352]

But i thought RBC don't have a nucleus as they have to store hemoglobin

[@PelletJamie]

So what is the Jelly like substance surrounding these strands of DNA?

[@edward_grabczewski]

What microscope are you hoping to buy for the microworldarchive.org project?

[@Microbehunter]

It's an Olympus BX53, LED, Plan Fluorite 4x, 10x, 20x, 40x, 100xoil DIC Prisms, one for each objective, Wollastone Prism etc. things that are required for DIC. It produces extremely pretty images, though.

[@edward_grabczewski]

@@Microbehunter Well Oliver, I can't think of a better person to have such a microscope. I'm sure you will do wonderful things with it. Good luck!

[@anamranixib5066]

Can u do a study on the plasma membrane f human rbc

[@brittonbercegeay6591]

I don't know man, You'll have to extrapolate on how dishsoap 'breaks open the cells' Also precipitation is the result of a chemical reaction of elements not the result of 'separating DNA from living tissue' Also, doesn't our mouth and entire digestive system literally always have various bacteria present?

[@prashanthn8467]

sir and one day I saw in my house that when i store water in a container for 3-4 days then i an see some white color floating on the water can you tell what is that

[@katlegomokgwakgwa3115]

can you use an alcohol based alochol instead

[@chemistrynotes5900]

How you can suspect that it s only dna it may be other cell orgenels

[@MielBrillant]

I've done too at my school! Although the result was different from yours , but it made me really excited😍

[@nicolaeionescu-kosa132]

Great day for me to see DNA live. Thank you.

[@sanaanasr9877]

Hi, this is really interesting. What if we try and see bacteria in that solution? Would this be possible?

[@Pustic2]

Oliver, where are you located?

[@armydw]

I wish i could put camilian DNA in me so I could camouflage!!!... is this possible???

[@bcpool417cp]

What about messenger RNA? Such as a corona virus? Just curious how they come up with the corona image..

[@jaimedpcaus1]

Which microscope are you getting?

[@enter.netinfinity6136]

Please make a video on how to match two dna

[@zachreyhelmberger894]

Nice work!! What happens if you rhythmically press on the cover slip for a while? Does that break up the clumps of DNA?

[@sheepyleepy..2806]

could the dna be with a smalllll drop of water or mixed in with a very tiny amount of water

[@ruchika3869]

Sir how can we extract dna in intact form ?

[@dennismoya4311]

It has to be done in a lab. However it is not intact. Once you remove the histones (proteins keeping DNA integrity) when you try to purify DNA, you end up with broken pieces of denatured DNA. However for lab purposes, even broken DNA is useful.

[@thereality657]

But how do you know whether your DNA or bacterial and other microbes DNA?

[@dennismoya4311]

that would be difficult to know with the steps he took. He used saliva; there are bacterial DNA mixed as well for sure. The only way to know if it comes from bacteria or him, is to amplify it and run the DNA code. However, his video is more about how to visualize DNA in a light microscope. The DNA is going to be mixed and from different sources for sure.

[@titan1286]

What microscope did You use that must be very powerful Is it by olympus?!

[@Microbehunter]

I am using an Olympus CH-40 and an Olympus CHA with phase contrast. Other microscopes should also work.

[@xioadingdou4524]

What dish wash solution is it?

[@shantichodankar6595]

Can we also use forceps to lift the DNA ?

[@reginacarroll8149]

What is used to actually see DNA strands?

[@Microbehunter]

electron microscope: but barely.

[@titan1286]

How much magnification do you need to see cells

[@Microbehunter]

You can see large cells (onion) already with 40x total magnification. Red blood cells can be seen with 100x and more.

[@Sol-ui2if]

Can you please put under the microscope rain water collected from plants? I wish to know why it contains red blood cells.

[@diamondhunters9782]

Can you do paper under it and see how its connected

[@sidhartville7151]

Paper is a composite of wood fibers with about nanometer to millimeter in length.

[@sidnelson9379]

Can you suggest a home available stain that would be more specific to DNA that would be fairly non-toxic? Since DNA is acidic, maybe there could be a basic dye (I am thinking that Malachite Green available in the pet section of stores for Fish Ich, but know it is highly toxic). Aloha from Big Island! -Sid

[@vinayakmalge5121]

Which tupe of microscope you use for see the DNA....??????

[@Microbehunter]

This was a phase contrast microscope. But these are not individual DNA strands, but most likely several bundled together. Individual DNA strands can not be seen under the microscope as they are too thin.

[@aaishahv5091]

Will using strawberry DNA also be visible under a microscope?

[@MissZenia1982]

Well how do they compare DNA if you cant see it with a microscope?

[@Microbehunter]

DNA can be compared by doing "DNA sequencing". This is a chemical process. The process is now done automatically using sequencing machines. When you have the DNA sequence of two DNA pieces, then you can compare their similarity. You can also compare it with the sequences stored in a database. More info: en.wikipedia.org/wiki/DNA_sequencing

[@fyohox742]

hypothesis: those strings/nets could as well be microtubules, or even recombining cell wall proteins. i do not trust alcohol to preserve long dna chains and certainly not bleach. maybe we should try this experiment by centrifuge

[@Microbehunter]

Alcohol does preserve DNA, Alcohol precipitation is the standard method for DNA isolation. Beach was not added, its oxidizing ability would indeed destroy DNA.

[@ggbond942]

does that mean when we wash our hands with soap our skin cells are broken up?

[@kcharan4837]

Which stain is better when it comes to plant tissue

[@UGCshakinahstouch]

You need a sugar saluting to open up cells

[@ayanbanerjee6610]

These may be are the microtubules or Intermediate filaments also'.

[@vloggerboyshekhar]

Sir which microscope do u use

[@roelzylstra]

@Microbehunter , Which microscope were you wanting to buy? Did you buy it yet? What makes it expensive?

[@patrumery5293]

Amazing as always. Thank you for all you do. This is my favorite video I have seen.