We Upgraded Our Microscope... Again!

[@journeytomicro]

Journey to the Microcosmos is supported by Brilliant.org. Go to Brilliant.org/microcosmos to get 20% off of an annual Premium subscription.

[@jacobfurnish7450]

Soil food web. Please do it. Explaining how important nutrient cycling is would be awesome!

[@martf4701]

Please tell me that atoms and electrons CANT be seen. Even with " electronic microscope ".. That they can only be REPRESENTED.

[@outlawscar3328]

Great. Stuck at 480p because you guys had to upload in 60fps again. There is absolutely no reason you should be uploading at 60fps unless it's an alternate link. I'm going to dog you about this until I die. It's so elitist and unnecessary.

[@pablogastonlanouguere9417]

​SFW pleaaaseee!

[@Redditard]

James OP :)

[@Glenport]

Hank accidentally defines life: "Bags of resilient bubbles."

[@swayback7375]

We can all relate

[@ChurchOfThought]

Solitons en.wikipedia.org/wiki/Soliton

[@Redditard]

True

[@dickJohnsonpeter]

Most living things aren't a bag of bubbles.

[@Apastorfield]

i prefer ugly bags of water

[@samudrajs5409]

Can we agree this is basically a science ASMR channel? Love Hank's voice

[@Ronin777z]

Yup, sometimes I use these videos to help me fall asleep lol

[@XanthumS]

Yeah I just watch it and hypnotized until end of the video

[@gabrielz8333]

XD lol I'm not the only one yay

[@ctrl5274]

Such a clam and quiet voice that help me feel like I’m under the microscope

[@paulhoward8300]

I fall asleep to it every night

[@1987zdavis]

Season 4: We've upgraded our microscope again. Season 5: We've got a scanning electron microscope. Fingers crossed

[@grieske]

Then you also need a sample preparation robot to vitrify the sample, as a SEM examines the sample in vacuum.

[@PeterVJaspersFayer]

SEMs are indeed wonderful, but not for living cells -- they require a vacuum, so no life, no movement (anhydrobiotic tardigrades excepted - but they're boring 😜)

[@bertusvanheerden]

Superresolution would be cool

[@guyjclark]

@@grieske Hi! back in 1974 when I was a Biology student at University of Illinois in Chicago, I was hired by the department to get SEM images of the insides of Euglena Gracilis. I'd take a drop of culture, and place it on a little square of double sided tape on the top of an aluminum stud which was used as the microscope stage. I'd freeze it quickly with a blast of liquid nitrogen, and then scrape half of the frozen drop with a spatula, and then freeze dry it. After sputtering some gold or aluminum on the now dried sample, (to better reflect the electron beam), we'd put it in the SEM and look for interesting things to record on a Polaroid camera fixed to a high resolution CRT screen on the microscope. The organelles of the damaged cells could be seen, having tumbled out of their cell membranes and could be identified. I thought it was wonderful that I could do things like this AND get paid for it at the same time, while a freshman in college!

[@Redditard]

@@guyjclark ohh

[@lavathor]

"Thank you for coming on this journey with us." "Thank you brilliant for sponsoring this video." Your forgetting the most important one... Thank you James!

[@tensorflow5777]

and mostest importantly thanks to Hank

[@o0Donuts0o]

One day, on this channel, you will hear the words "It's your boy, Raid Shadow Legends" Nothing escapes Raid Shadow Legends.

[@journeytomicro]

@@o0Donuts0o You will not hear those words on this channel.

[@jzlazy1057]

@@journeytomicro Amen

[@kern9422]

@@journeytomicro this sounded like a downright threat to me and i got secondhand intimidation from reading this lmaooo

[@vitamink1028]

3:27 this shot of the Hydra is so breathtaking! (it looks like there's gold glitter)

[@MatthewGaydos]

We've got a whole Hydra episode coming soon with footage just like that!

[@vitamink1028]

@@MatthewGaydos Yayy! I'm looking forward to it.

[@GordonFreechmen]

Geez, you bois keep outdoing yourselves. We're travelling the universe just through our screens.

[@Dimitri88888888]

We're traveling the universe just backwards instead

[@willmcconnell6008]

I can't wait for more fluorescece microscopy! I'm working in an academic lab where we're designing fluorescent dyes that change color based on their environment (Red when inside a membrane or orange when it's exposed to the cytoplasm). We use a computer to simulate/calculate the electronic properties of dyes to get an estimate of if the dyes could change color (Solvatochromism). It's neat stuff

[@spudato961]

I love this channel

[@travis_approved]

+

[@woodstockjon420]

Me too!! 😎 Who possibly clicks down thumbs?😱

[@o0Donuts0o]

When my boss asks me what my objectives for the year are I’m going to say 100x and walk away. My objectives are beyond their understanding.

[@DanceSeek]

And your boss will remember that response when doing performance reviews and promote someone else instead of you. Maybe that's what you want, I don't know, but consider what hill you want to die on when tempted to make a snotty comment.

[@o0Donuts0o]

"the hill you want to die on". When that phrase is uttered you know you are dealing the kind of person who needs to be the smartest person in the room, but is clearly the dullest.

[@DanceSeek]

@@o0Donuts0o "My objectives are beyond their understanding." When that phrase is uttered you know you are dealing the kind of person who needs to be the smartest person in the room.

[@DanceSeek]

@Anonymous D?NGO I did consider the possibility that it was a joke. However, since this is text I couldn't see any twinkle in his eye. Also I feel compelled to ask the next question in these situations.

[@o0Donuts0o]

@@DanceSeek The serious one when an obvious joke is made. Is this the hill you want to die on? Nyuk nyuk nyuk, eye roll.

[@vitamink1028]

I'm excited for the future videos with the new microscope! Glad to be a part of this journey as a viewer.

[@journeytomicro]

Don't forget to pick up James's book if you want to hold the microcosmos in your hands! www.penguinrandomhouse.com/books/667123/the-hidden-beauty-of-the-microscopic-world-by-james-weiss/

[@einsteinx2]

This channel is criminally underrated… I can’t believe each episode doesn’t have 10x more views at least!

[@eviehankins8223]

Seriously. It's an amazing channel

[@tensorflow5777]

am here since the very first vid but havent seen 90% of vids. theyre too much. a two hour well-structured documentary style ofall old content would be nice

[@swayback7375]

Nerds are scared of KZbin, well boomer nasa nerds anyway. They think they know what’s here when they’ve never watched a thing, maybe the explore section and then they got really scared

[@madamsloth]

Same

[@blaeks]

good things take time.. this is actually fast. the world is burning, society is atomized, progressive victimhood is the plutonium of our demise

[@regular-joe]

Hank and Team - this is beyond exciting, so awesome that we have access to all of this through you. THANK YOU!!

[@simone222]

Congrats on the new upgrade! Your captures will for sure be even more spectacular with it.

[@RyuSujin]

Got to the end and I thought "the video is over already?" and realized 10 minutes had just flown by. Now *this* is quality content. You have a new subscriber :D

[@fauxbugnl9968]

It's rare to see channels that have 0 dislikes, especially of your size. But how can we expect such a thing from such an exciting channel. As a student busy in medicine, this is absolutely incredible to see. Many thanks for the continued uploads.

[@Sevenigma777]

8:44 is proof "as is the micro so is the macro". It looks exactly like viewing a galaxy or something similar in space.

[@dracoaestas]

That last image of the dying copepod is profoundly beautiful. The finality of and transformation in death, the visual reminiscence of solar flares or nebulae, the fascinating chemistry and physics of fluorescence. It's incredible.

[@OhanSmit]

Thank you for showing us a journey into a world that would otherwise go unnoticed!

[@Beryllahawk]

What a wonderful new view we'll be getting now

[@AmblesJambles]

I remember getting the chance to use fluorescence microscopy in university, it's fantastic. In my case we tagged specific proteins with fluorescent molecules so under specific wavelengths we could identify where the proteins were inside the cell. It was a very rewarding project! I can't wait to see what you folks do with it!

[@burtbackattack]

You got to love Hanks soft, soothing voice in these videos.

[@arko3822]

may this channel grow more ....awesome work guys

[@darlantro]

8:20 one of the coolest things to do with video through a fluorescent microscopy is using a triple cube. That would be my recommended purchase for you. A lot of younger microscopists don't know they exist and think you can only see one fluorescent channel at a time. Some older confocals had them as a quick check cube of dapi/fitc/tritc before you then switch to the lasers, and some newer high-plex fluorescent IHC slide scanning systems use triple cubes now-a-days for faster throughput.

[@luisaparodi8571]

Congratulations James! Glad to kow about the microscope update!! 👏👏👏❤😊

[@mentalizatelo]

Kudos on your new microscope! Love your channel!

[@SKERLECK]

I don't get to watch this as much as I'd like to but it's definitely one of the best channels on KZbin. And you're actually doing something!! You're not just wasting 10 minutes per video. I love it, truly

[@xcruell]

That last picture is absolutely stunning! I need this as a live wallpaper or something :D

[@Bobsry16]

You guys are a blessing! Thanks for the visuals of these tiny creations on a big scale.

[@JohnPaul-yf9xd]

I would love to say that I have seen all of your episodes, but I know that you have many more.I am always amazed at your work and am always waiting for your next masterpiece

[@robertsretrogaming]

This channel is brilliant. I would like to see an episode (or more) about the motion of cilia and how they generate the different motions of the organisms or how they are used to manipulate the environment to do things like draw in food. Exactly how that works is not clear to me, and having you all explain it would be great.

[@JamsGerms]

That’s a great idea, thank you! -james

[@daveb5041]

*MY favorite part of micro101 was taking a drop of pond water and seeing a whole ecosystem, marveling at it for an hour then chucking it into the bio hazard bin where all die as soon as the water dries out. I often wonder if aliens are doing the same thing with earth* .

[@zorbozog]

The imagery! It's so crispy and beautiful!

[@maboroshi2550]

In a small machine we hold in our hands... we can see things bigger than ourselves amd smaller than we can observe. This took my breath away for some reason. Thank you - this truly made my day!

[@ethansuppes6285]

Probably the most amazing channel on KZbin. It's Educational, professional, and still finds ways to make me laugh. Its because of this channel I got back into microscopy and culturing microbes.

[@jefferysmith4753]

I have always been fascinated with the unimaginable size of the universe. This channel has helped me to appreciate the beauty and wonder of the microcosmos as well.

[@Shaden0040]

James can you show us what lives in an aquarium? such as infusoria, auf Wuchs, and micro crustaceans? Thanks.

[@Mindspiel]

I'm happy for you and yours new equipment. I learned so much from following this channel. Everything i learned now i'm transferring to my kids. keep up with good work

[@strongerandwiser2023]

8:45 is absolutely Stunning. I could watch that for hours.

[@benmcreynolds8581]

OMG that shot right before the Brilliant ad plug comes up is amazing! It looks like a galactic bubble blob nebula area type thingy.

[@selfawaretrashcan4594]

One of the most entertaining channels on KZbin

[@limiv5272]

I love this channel! This is all sooo pretty and fascinating and educational and well done

[@Macro-photographer]

I purchased a microscope and became a member of the Royal Microscopy Society purely based on your channel! I just wanted to add that having done some amatuer astronomy and sold all the kit to fund the microscopes, the two hobbies are visually very similar in many ways, especially polarised and dark field illuminations. Thank you for bringing me into an amazing hobby, and being asthmatic I don’t get chest infections now by being out in the cold night air!

[@SCP-up4ot]

idk why, this reminds me a lot of my classes. I dont actually take microbio, but half my course is abt plants, so we get to look at plant structures etc. One lesson, I brought some water from my fish tank to class and used the microscope, I saw a small green single celled organism in there, and it was pretty cool. Funnily enough, abt a week after I got recommended this channel. Thanks for all the cool biology I'm able to see.

[@Widderic]

I love the internet. I'm a huge fan of the cosmos, but you've got me hooked on the microcosmos, just as interesting and mind boggling. Looking forward to when you get over 9000. Subbed!

[@charlesdye8367]

Thank you all for this. I was a medical lab tech in the Army so many years ago. I had all of these tools at my disposal and used them extensively to dive deeper into the cells of our bodies and the cells of those who wished to live in our bodies. Since retirement I have probably missed this the most. Many times my children would come to work with drops of water or small creatures. Enjoy this while you can. 🥲

[@miriam7872]

Ohh! I just learned about fluorescence microscopy in my biofilm systems lecture last week!! That’s so exciting! If you get some different dyes for EPS and nucleic acids that fit the wavelengths of your microscope, maybe we can see a bit of the structure of those complex and wonderful bacterial communities Edit: thermofisher’s spectraViewer could be a nice tool to look at the excitation and emission spectra of different fluorophore dyes! I think it’s technically designed for more complex applications like confocal laser scanning microscopy but it could still be of help

[@Yezpahr]

8:42 wow... that looks amazing! Almost like a timelapse of the macro cosmos.

[@PiterWandercleydson]

thanks so much for sharing such gorgeous images

[@Puleczech]

Thank you for going into such detail, it is really great to peek behind the scenes. Amazing work you do!

[@Scinfinity]

Wow... It is beautiful!!!

[@AbrahamSamma]

This is absolutely beautiful and fantastic stuff. 🙂

[@sa.8208]

4:04.. the name, the voice, it all just clicked. and i love it.

[@gerdahuertas26]

Congrats on your new microscope! It is so awesome to view all these microorganisms with all these techniques. Take care and keep safe!

[@TedToal_TedToal]

Amazing! Beautiful! Stunning microscopy! Incredible structures on these organisms, coming so clearly into focus! I would think so much could be learned just by looking!

[@Task562]

Most wonderful thing to wake up to and watch is this channel

[@AstroProductReviews]

Outstanding! Those videos have to be the brightest/clearest I've ever seen. I'm currently shopping for a fluorescence microscope (once again!) :)

[@TheCgOrion]

Congratulations on your recent upgrades, and to your success with this series.

[@DracarmenWinterspring]

7:19 - that's beautiful, it looks a bit like a painting showing up stroke by stroke...are those the wing's scales? are the colors what the wing would look like in visible light, or not necessarily?

[@jitgtij]

Wow, bags of very resilient bubbles, that's was awesome, that's basically all cells

[@louieKendem]

I'm in love with this channel and the entire production team. PLEASE never stop, you're a blessing

[@absentisomnis8650]

its lovely to see how this channel grew on the development side

[@nanow1990]

I’d just like to add, it’s great to know you were able to get it done cuz there is a price to pay in time and money for getting a brand new microscope. It’s hard work.

[@phantasticdrago230]

“MicroCosmos” I love that.

[@donatodiniccolodibettobardi842]

Thank you, for guiding, writing and recording this journey for us.

[@Chira92]

As a lab technician specialized in microbiology this is so exciting and fascinating to watch. The images are amazing and I can't wait to see what you guys have in store.

[@orangejoe3864]

Been following since day one. The fact that you don't already have millions of subs is subject to how much you are truly needed for general knowledge. Thank you!

[@joeldunlop3838]

I love this show! Hats off to removing the ads👍 keep it up James and the team, I watch this show with my son

[@artmakersworlds]

How very cool indeed. Thank you guys for these videos.

[@l.mcmanus3983]

This brings me back to my university days, and a research project I was working on. I was able to attach a GFP gene to the end of a transcription protein and inject that DNA into C. elegans eggs (still in the adult nematode) and then integrate that modified gene into its DNA. This was before CRISPR was widely available so it was time consuming and tricky business. But it was really neat to basically see gene expression happening.

[@NewMessage]

Apt, considering how objectively amazing this channel is.

[@richardlandrum1966]

Awesome news guys. Keep em coming.

[@AtomizedMass]

This is amazing and brings me much joy to watch. Thank you!

[@joannarutter]

It's so beautiful it makes you happy to be alive.

[@MissNebulosity]

I subscribed the third week after you created this channel, and it has been one of the most AMAZING things to watch this channel grow beyond half a million subscribers!!!

[@THEinSEnDeaieri]

I fn love that you guys get to do this.

[@CombatKing1237]

the shot of the dying copepod was breathtaking, truly! some real scenes to inspire creativity here

[@gustavgnoettgen]

So beautiful footage, all of it

[@rosesacks7430]

enjoyed the journey, thanks for sharing 👍

[@rickseiden1]

5:08 The tetra-whosie-whatsits look like stress balls that are basically bags of little balls that slide around inside.

[@travis_approved]

Seriously love to see this channel succeed

[@troyclayton]

Congratulations on the new objectives! 7:19 Why does the moth wing appear to be 'growing' along the bottom edge?

[@subhadas]

8:41 this shot immediately reminds me of galaxies. Imagine every galaxy is a just a space organism!!!

[@kristaliaastari2856]

Will you guys be doing another run of those microscope’s or are you not going to make anymore?

[@bengoodchild883]

Nice work! Seeing the 1000X DIC of the insides of these microbes will be wild! Glad you are having fun :)

[@PutuDharmaMahaYusa]

Man, any shot so just work as a wallpaper, it was so beautiful!

[@fabrestervis]

glad i found you, glad you upgraded. subscribed and excited for more content :D

[@inarigitsune]

This microscope is now pretty much the same one I used in my research before, though the ones my lab had was from a different manufacturer. These microscopes are expensive and impressive. Fluorescence microscopy is always fun an interesting. Warning on plants and fluorescence, they tend to be so bright it's hard to see anything else especially under GFP settings, unless you have something else actively making fluorescence like GPF. You really need a control to dial things down to base level and then see if there is anything above that to check for any intentionally produced fluorescence - by plant, microorganisms or animals.

[@Globbo_The_Glob]

I'd very much enjoy a small note on shots in future telling us what technique is being used along with the magnification, provides some nice context to what's being shown. :)

[@handle8handle2handle2]

thanks for this video this helps me know for sure what magnification i can reach with my microscope i will be purchasing

[@SunilKumar18]

This is so bloody beautiful! Absolutely love it.

[@theawesomer]

Amazing as always! Sharing on The Awesomer.

[@temujinay8831]

Thank you for the free education guys , love your work.

[@danieldishaw7524]

Im just glad i found this corner of the internet, journey to the microcosmos has been a stable thing to go to when i want to relax for a long time now

[@Scinfinity]

Your voice is very soothing

[@bjarnes.4423]

I usually don't but I ramped up the resolution to max for that Georgious detail. Keep up the great work!

[@comicsansgreenkirby]

When do we get a second chance to buy the Microcosmos BGM? I missed the first one :(

[@greggorrell]

Love it, can't wait to see the content you guys create with the new kit!

[@gregoryjordan3224]

You should try some of the cheaper fluorescent dyes - DAPI, Acridine Orange etc for nucleic acids - would be cool to pick out the nucleus on some of the larger eukaryotes in real time. Another good and cheap one would be chlorofluor white for chitin in some of the tiny panarthropods like tardigrades.