Minimum Inhibitory Concentration(MIC)|Minimum Bactericidal Concentration|Post Antibiotic Effect

The Minimum Inhibitory Concentration (MIC) is defined as the lowest concentration of an antimicrobial ingredient or agent that is bacteriostatic (prevents the visible growth of bacteria). MICs are used to evaluate the antimicrobial efficacy of various compounds by measuring the effect of decreasing concentrations of antibiotic/antiseptic over a defined period in terms of inhibition of microbial population growth. These evaluations can be quite useful during the R&D phase of a product to determine appropriate concentrations required in the final product, as the concentration of drug required to produce the effect is normally several hundred to thousands of times less than the concentration found in the finished dosage form.
The minimum bactericidal concentration (MBC) is the lowest concentration of an antibacterial agent required to kill a particular bacterium. It can be determined from broth dilution minimum inhibitory concentration (MIC) tests by subculturing to agar plates that do not contain the test agent. The MBC is identified by determining the lowest concentration of antibacterial agent that reduces the viability of the initial bacterial inoculum by ≥99.9%. The MBC is complementary to the MIC; whereas the MIC test demonstrates the lowest level of antimicrobial agent that inhibits growth, the MBC demonstrates the lowest level of antimicrobial agent that results in microbial death. This means that even if a particular MIC shows inhibition, plating the bacteria onto agar might still result in organism proliferation because the antimicrobial did not cause death. Antibacterial agents are usually regarded as bactericidal if the MBC is no more than four times the MIC.
The post antibiotic effect (PAE) is defined as persistent suppression of bacterial growth after a brief exposure (1 or 2 hours) of bacteria to an antibiotic even in the absence of host defense mechanisms.
Theoretically, the ability of an antibiotic to induce a PAE is an attractive property of an antibiotic since antibiotic concentrations could fall below the MIC for the bacterium yet retain their effectiveness in their ability to suppress the growth. Therefore, an antibiotic with PAE would require less frequent administration and it could improve patient adherence with regard to pharmacotherapy

[@priyankatakawane8950]

Superb teaching...👍

[@chiraggabani0hfg814]

in case o culture positive where i will get MIC and MBC levels? or are they fixed?

[@mudassariqbal6874]

Thanks sir 🥰

[@akshatavaidya8281]

Can someone please tell me if Sub inhibitory concentration n MIC are the same ? .. If not , can please tell the difference ?

[@rampraveshpurbey2099]

Nice explaination thank you sir 🙏

[@TutorBoxChannel]

Always welcome

[@VishalKumar-do3ol]

Vary nice sir

[@TutorBoxChannel]

Keep watching

[@umeramjad2463]

sir 13 35 pe ap ne kaha ha the drug 2 become static . pls elaborate

[@nandamahajan1701]

Very nice lecture.. thank you sir

[@TutorBoxChannel]

Most welcome

[@anubisht4522]

I think etest is diffusion n dilution method.... But u have taken it in a solution... Plz clearlify the concept

[@vocalistharshi]

👍👍👍👍well explained

[@TutorBoxChannel]

Keep watching

[@akashpokhrel8361]

thank u sir

[@TutorBoxChannel]

All the best

[@DSB277]

Sir aap ek din bhut bde you tuber bnoge itna aacha concept clear krte ho🙏🙏

[@TutorBoxChannel]

Thanks for your motivational words.

[@DSB277]

@@TutorBoxChannel thanks ki jarurat nhi hai sir Salute h sir aapko🙏🙏 Plz microbiology ki or videos bnao

[@nishantsen6820]

Yes sir make videos on important topic of micriobiology

[@TutorBoxChannel]

check microbiology playlist

[@surajlohara5239]

Thank you so much sir for video 🫶🫶🫶❤️❤️❤️

[@TutorBoxChannel]

Most welcome

[@shwetapotdar6480]

Sir your explanation is very good ..but you didn’t explain how to calculate MBC value of a drug

[@TutorBoxChannel]

for the i will make a separate video

[@JasdeepGrewal-oq8yf]

Sir MLT key kon Kon sey competitive exam hotey hai guide kar di jiye please 🙏🙏

[@TutorBoxChannel]

ok