How do you determine what concentrations of the antibiotic to use?
@RahulMhaskar3 жыл бұрын
Thank you
@Emerypharma Жыл бұрын
You're welcome
@shymabio4 жыл бұрын
How much ml every well from the plate???????????
@imrocknreeling3 жыл бұрын
how much 0.5 McFarland adjusted bacterial broth needs to added in how much antimicrobial solution?
@clementpaulodc2 жыл бұрын
Colony inoculated in sterile saline and read in turbidometer.
@simonelemer51592 жыл бұрын
Usually the MIC protocol requires to adjust the bacterial conc to 10 to power 5 ufc/ml via McFarland standard comparison of solution turbidity (you have to make an internal standard) and usually the inoculum size (v/v) is 10 % of the final solution volume. So when using a 96 well plate, the final volume in each well should be 100 microliters to which you must add 10 microliters of 10 to power 5 ufc/ml dilution.
@AhmedMohamed-xy3mm2 жыл бұрын
Can anyone attach the pdf of the book she referred to? The "M100S" titled book
@Emerypharma Жыл бұрын
it will be difficult to attach the pdf of a book. Thank you for watching. be sure to subscribe to our channel www.emerypharma.com
@juggernaut93642 жыл бұрын
Can the 96 well plate be kept in orbital shaker incubator ? Can we get the results if they are being kept at the orbital shaker incubator ?? Can I plz get a reply ??
@Emerypharma Жыл бұрын
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@sukalpachakraborty5272 Жыл бұрын
Good
@Emerypharma Жыл бұрын
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@stephenarowosegbe86763 жыл бұрын
Am confused here which of the medium was this performed was it broth medium or agar medium.
@simonelemer51592 жыл бұрын
How can you be confused this method in microplates can only be used with broth media, agar will solidify as you make your dilutions and add your bacterial inoculum. So all the MIC assay is realized with broth. In addition for a easier evaluation of the MIC results after incubation, an cell oxidation activity reactive should be added i.e.: resazurin which is a blue dye, and wich after a further 2h of incubation will change color from blue to pink if there are active viable cells
@Emerypharma Жыл бұрын
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@zainabfatima79523 жыл бұрын
Turbidity was not clearly visible
@Emerypharma Жыл бұрын
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@phoolkumaripandey93282 жыл бұрын
Job required for microbiology fresher
@Emerypharma Жыл бұрын
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@turnipsmoothie66414 жыл бұрын
in 2 ways. microdilution and microdilution. im ded XD
@hafsashereen0064 жыл бұрын
she said macro and micro lmao 😂
@imagisma4 жыл бұрын
@@hafsashereen006 loll I had to repeat it 5 times
@Al-em8tp2 жыл бұрын
I hate microbiology.
@Emerypharma Жыл бұрын
sorry to hear! studying microbes is very important to our health. Thank you for watching. be sure to subscribe to our channel www.emerypharma.com