Counting Cells with a Hemocytometer

[@MrAllacazam]

i wish every single labs out there had beautifully edited constructed with great explanations like this... would definitely make uni life way more interesting... amazing video, hats off!!!

[@thefenerbahcesk4156]

This isnt beautiful. It's boring and tedious as hell.

[@Pranav_Bhamidipati]

Wouldn't it be better if you did them yourself?

[@umazajacker8182]

​@Gatlin Ronnie Pretty sure no one here cares. Promote it at instagram hacking tutorial, more people will care

[@rengeshb]

Many have a question with regard to why it is 10^4 (@5:42) for concentration of cells per mL: Now recall/remember Watch video @1:42 to what they say with regard to volume at each square. It occupies a volume of 10^-4 mL i.e 0.0001 mL = 0.1 µL. Therefore for calculating - concentration of cells "per mL" make this 0.1 µL to 1 mL (multiply with 10^4 to get 1 mL). Hope it helps

[@Mushimion]

Thanks I was struggling with that. Cheers

[@ritwikabhadra6383]

Thanks a lot❤

[@nihedhb]

Why she said each square contains 10^-4 ml even though 200ul/9 squares gives 22 ul in each square we should have 22 ul

[@mdumaguing]

I've watched so many videos on how to count the cells and you are the best ever who explained it properly, I can understand you very well with your speech and you explained it properly, thank you,

[@Obalatan2010]

Excellent work. It's crystal clear and systematically explained. Thank you for putting this video up.

[@shingonakai]

Your videos have given me great teaching aids to students who are new to cell culture! Thank you!

[@hellofriends40]

Very good video, but one mistake. At the end, she says that 94.7% of the 216,000 cells/mL are viable. However, she uses the number of viable cells (54) to calculate average number of cells/square. If she had used the number of total cells (nonviable + viable, or 57) to calculate cells/square, then in the end it would make sense to take the final number and say that 94.7% of those cells are viable.

[@ahmedsalah-oc3vl]

amaaaaaaaaaaaaaaaaaaaazing ,,,,, really amazing excellent photographing ... direction.... sound effect... commentary ... subtitle .. perfect timing. science is different actually

[@BrandonRosenstrauch]

Wow, this was well put together. Great job!

[@amailyakter6589]

excellent!! today I got the total process of how to calculate cells, thank you Miss for your wonderful video

[@masterpee2328]

Thank you for saving me. No video could explain it better.

[@kerensahardesty9851]

What crystal-clear instruction. Amazing!

[@swahifaabdijuma2782]

Thank you so much I have 4 hrs tp submit my r lab report you saved me thank youuuuuuuuu❤❤❤❤❤❤❤❤❤❤

[@TheTypicalKid]

Thank you for the help. This video has now made my understanding of Hemocytometers even greater and therefore has also made my day better.

[@lilicsb]

This is the best video about the topi... so easy to understand :) Thank you so much!

[@EDUARDO12348]

Thank you very much, now am ready for lab class tomorrow here at MD anderson

[@mr.cobuelo1405]

Hey it's been 6 years and I've always wanted to learn at MD Anderson. What are you doing now?

[@ramlahassan9761]

This helped so much! I can thank you enough! Crystal clear! Keep up the great work:D

[@ivanmikhaylov1103]

Crystal clear? Why non-viable cells look darker than their environment?

[@jimines6422]

Wonderfully articulated and easy to understand, better than many of my teachers.

[@jimayla7446]

I have Diagnostic Virology practicals in two hours. Thanks for the video

[@MarianaDiaz-vo8wb]

WOOOOW, It's super clear!, Thank you very much for your support!

[@Iinachanel1973k]

Really enjoyed it! Excellent quality throughout! Thank you so much! xoxo

[@adarshpatel7571]

This was the best video ever

[@palsnicky]

Really easy to understand and great use of simple graphics, I'm just looking into yeast counts for brewing. If I can follow this anyone can, thank you.

[@mikestout2506]

Thank you. This is just what I needed.

[@shajidislam2222]

But when we do subculture how much amount of media or cell suspension will be required ..please explain that one also...and thanks for your good explanation

[@sexxyfj]

where did the 10^4 come from???

[@jelle902]

Even though it has been 7 years, the 10^4 comes from volume of suspension in each square (which is 10^-4 mL)

[@mjunchai630]

@@jelle902 why the calculation state that is 10^4? why not 10^(-4)

[@jacobjackson3372]

@@mjunchai630 so before you reached that step, it was calculating the concentration of cells in a square (which as he said is 10^-4 ml). We want to express our concentration in terms of cells per ml though, so we multiply by 10^4, essentially doing unit conversion.

[@alexaoctaviano5038]

@@jacobjackson3372 i dont get it can u elaborate it more pls

[@anastasiiakutulska1245]

@@alexaoctaviano5038actually, this is because we convert microliters to mililitetrs:)

[@nathanstephens9442]

We use this video in an introductory cell biology lab course, it's very informative and helpful! However, the linked portion where you can print off a certificate at the end appears to be broken. This has been a great mechanism for giving students credit for watching the video. Do you have any plans to make that part active again? Cheers!

[@nathanstephens9442]

Ahoy! Apparently there's no way to tag a channel in here nowadays and therefore no way that I'm aware of you get your attention, BioNetwork. So I'll just reply to my own comment and see what happens. Please take a moment to address the concern in my above comment. Thanks!

[@sagarshrestha5800]

@@nathanstephens9442 email?

[@ahmadtalsaadi]

Thank you, this is the best presentation in this regards

[@KaiusKing]

Good Video!

[@shalminpohtam0999]

Super clear explanations.Thankyou.

[@roshanhemantha1637]

Thank you so much. This video is a very interesting and very important video.

[@texNoz]

Why fill both squares? You've only counted one.

[@MegaCadette55]

Good question!

[@malem3ingudam264]

it to work as a duplicate and increase accuracy but she had forgotten to do so while putting an unnecessary google

[@shivichaudhary5960]

Amazing explanation

[@albert-zn3bp]

very clear and easy to understand.

[@sara97979]

thank you soooo much for the great, smooth explanation

[@koushiksarker1977]

Nicely presented.

[@tylermacdonald8924]

Why do they multiply by 10^4 when calculating for the concentration of viable cells/ml?

[@a.w.4708]

Because she counts number of cells per ml and cells she has count was in 0,1 microl, I guess

[@mollyfox9394]

The reason you multiply by 10,000 is because each square contains 0.1 uL, so x 10^4 converts 0.1 uL to 1 mL

[@smtiquia1]

I have similar question. Should it be 10^3 (the factor that converts ul to ml)?

[@pankhuriaswani58]

U will get the same answer whether u convert ul to ml because both the numbers having same units.....

[@asia2853]

Why does it have 2 chamber but counted only 1 chamber? So what's about the other one?

[@ogalber]

You can for example count two different types of cells. In this case they only had one cell line, so no need to use the other side =)

[@aishwaryaray8296]

Thanks for such a great explanation ...really worthy enough

[@ddneb]

I love it...Good refresher.

[@elizabethduffy1958]

Really informative video! Although I understand the calculation for the percentage viability, I'm just wondering why the average number of cells per square (10.8) isn't rounded down; as it is not possible to have 0.8 of a cell? Thanks!

[@jpsmjs]

Ultimately, the purpose of the calculation is to determine the concentration of cells. It is true that it is not really possible to have 0.8 of a cell. However, the exact number, 10.8, is used in the calculation for the concentration of cells for a more accurate result. Hope this helps!

[@enasmohd2508]

Thanks that really would help alot.. what a simple yet great explanation 👌👌

[@The_UNKNOWN_MAN_777]

Bro can you tell me from where the 10 raise to power 4 came...

[@neithonosmani9783]

@@The_UNKNOWN_MAN_777 I found the video very helpful but I also wanna know that too

[@anuradhawijethunga5880]

This helped me understand the technique really well..Thank you ❤️

[@ivanmikhaylov1103]

Why non-viable cells look darker than their environment?

[@a.w.4708]

Very clear, very useful. Thank you :)

[@AsadKhan-dv1vo]

How much volume of cells plus trypan blue are you adding to the cytometer chamber

[@venkatanaidukarri6527]

very good presentation

[@achalrathore5564]

Thankyou so much for this amaaaaaazing vedio👍👍👏👏

[@beinghuman4457]

when to use trypan blue and when to use RBC diluting solution ?

[@aslhanv6531]

How much microlitre we should put on one side?

[@marcelaandrealauzon940]

what is the publication where did you get these calculations? it should be in the description. Thank you

[@nichnank]

Thank you so much. This is really helpful.

[@arkanshsharma850]

Time saver !!!!!!!!!!!!

[@Jarrod_C]

why multiply by 10000 at the end? You never explain that.

[@mateuszklimczewski1881]

it's to change mikroliters to mililiters

[@Koodles33]

@@mateuszklimczewski1881 the conversion for uL --> mL is 10^3, not 10^4

[@tradingwithtrev]

I have also seen Hemocytometer spelt Haemocytomer and Hemacytometer. Can anyone please confirm the correct spelling? Thanks

[@sheri-.-]

The background music just gives it a 😌 vibe

[@ruksanakhan4989]

Very helpful video....👍

[@li-ion6228]

hi question! if they so much as touch the bottom and right one, are they already excluded from the count? like if they touch the first line of the bottom and right lines. thank you!

[@kariiamba7324]

Thankyou for this informative video. Appreciate it.

[@malavikasathian]

Well explained video and interesting. Truly helpful. Thank you!

[@NCBioNetwork]

Glad it was helpful!

[@zohrehestaki]

How we take into account the original volume of cell suspension?

[@ranimmira3498]

Thank you, Finally I understand it

[@isan123]

Does anyone know why it is multiplied by 10^4 ??

[@danjbundrick]

Irram Sanna Because each large square houses 10^-4 mL of liquid. You multiply by 10^4 to find the number of cells that would be in a whole milliliter of liquid. This makes your units: cells/mL

[@ayeshiabeavers8060]

Daniel Bundrick Thanks for that response. I was wondering that too and no one at work could tell me.

[@pearlrodrigues6073]

thank you so much... its really awesome...

[@sazanismael3688]

Great explanation !!!

[@Dadoimp]

great job guys! love your video!

[@MrApitipiti]

thanks for this-- very helpful!

[@antebellum606]

Is that a standard light microscope? In my readings it says that a fluorescent microscope is required to detect the dye that distinguishes living from dead cells.

[@afonsomendes92]

She used Trypan Blue. It is used with a light microscope.

[@houriabenarradj8260]

Thank you perfect video and good eexplanation all my best

[@rebeccamaepatricio1079]

THANK YOU SO MUCH!!! viable for learning

[@samikshyabhattarai5377]

I don't get that you've used average # of viable cells/square to measure the concentration and at last, you mention that 94.7% of that concentration are viable. Shouldn't you be using total # of cells per square and then use it to determine total concentration, of which you'll have 94.7% viable cells concentration?

[@D.f93]

Well explained.

[@NCBioNetwork]

We glad it was helpful for you.

[@anondoggo]

Thank you so much!! This is very helpful!!

[@ataurrahmanbhuiyan6858]

Easy to understand, Thanks

[@RehaAbbasi]

This is very nice. Thank you

[@NCBioNetwork]

Glad you like it!

[@SaddamHussain-gg5ws]

thanks for this vedio because it is very helpful for making dilution

[@thehypercasual385]

Great video!

[@factstrip786]

Mam can you explain if the RBC count is more in corner than centre so we only count center's RBC and not corner then report me make has cause an errors to diagnose patient 🤔🤔🤔🤔🤔🤔🤔🤔. Please tell about this

[@eddyatay9213]

So what's the normal range for WBC using hemocytometer?

[@erdenechimegdashzevge2674]

Thank you ver much. this video is very helpful.

[@Rikka_V1]

Hate this device. I couldn't even find borders in between the 9 squares probably because the slide was old,faded and my examiner didn't believe it saying I didn't adjust microscope well enough.

[@bassantmohamed7436]

Is 10^4 is the depth factor?? At calculating the cell number Anthor question ,I know that if we have small cells(bacteria) we will count at the central square only and if it large cells(fungi) we will count the four squares on corners only, is that true?

[@nafisasambo8]

A question please why was the concentration mulitplied by 10^4

[@lemyafinder289]

please where are you doing this manip? is it possible to realise a work there...i'm looking for a cell culture lab for an internship.

[@benjaminmustonen9006]

Is there a specific type of pipette you have to use?

[@NavjotSingh-tx3tn]

thanks you for such a beautiful video , its an interesting task.

[@ronanokennedy8592]

Nothing about creating newtons rings undercover slip for accurate counting .?

[@ej9100]

Hello in 4:01 why is it that the cell in the 3rd position on the very last row was not included in the count? Thank you😅

[@Sama4tx]

wow! excellent job

[@ЮрицЩербаков-ч1б]

Can you tell me, please, what model of microscope do you use in this video?

[@afidafatma4789]

How to count sel density microalgae that has spiral form like Spirulina with haemocytometer?

[@zainabnida]

Which microscope you used for this experiment? kindly let me know

[@MrErluz]

Nice vid. But at the end you said that there are 216.000 cells/mL and 94,7% of them are viable cells. This would be true if you calculated the concentration with the average number of cells per square (57/5 = 11.5) and not with the average number of viable cells/square (10.8). Correct me if I'm wrong but the 216.000 cells/mL are only the viable cells.

[@teddyboron688]

Everytime we should count the cells in the five squares or it is the experimentalist's choice ?? Please reply.

[@onurtatar3268]

Can I use hemocytometer while counting a bacterium

[@dr.jakirsbiologyhub1903]

Thanks, it is amazing to learn.

[@fizakyeomz-6744]

thanks for the great video:) I wanna ask, is there any other alternatives for trypan blue? like common stains that easy to prepare or obtain? can methylene blue be used instead of trypan blue?

[@kosheeka]

Great question! Methylene Blue is unsuitable for cell viability as it will stain both live and dead cells equally. You can try automated counters, fluorescent dyes (propidium iodide), or colorimetric assays (MTT, MTS, WST-1) instead. However, all these methods will require you to have specialized equipment to test the viability of cells. Trypan blue is a much cheaper alternative.

[@fizakyeomz-6744]

@@kosheeka thank you so much for the answer🤧

[@irishguy200007]

Can anybody please explain, I want to count cells with computer by taking an image of the simple and I need to know what chamber one uses to do this? Am I correct in my understanding that I require a counting chamber but not a hemocytometer?? When I look the term counting chamber it keeps comming up with a hemocytometer but I don't desire to manually count cells.

[@tugbailaybaran8690]

kzbin.info/www/bejne/Y3eWgY2gbt-iY7c

[@irishguy200007]

Is there a chamber not a hemocytometer to hold a known stained sample to photograph

[@firozahmad1653]

I love this video

[@s.c3855]

I always has a shaking hand when doing this

[@SaddamHussain-gg5ws]

any vedio about conidial production and measurement of vegetative growth of fungi

[@zaAljoman]

Hi Is this suitable for sample has oil?? And the trypan blue solution is suitable for sample has oil??