PCR calculation

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#PCR #calculation
#PCRcalculation
‪@PreetChhinder‬ ‪@WellnessGurujimegacamps‬ @PCR calculation
@pcr preperation
@molecular biology preperation
The common volume of a PCR is 10,25, 50, or 100μL.
Although larger volumes are easier to pipet, they also use up a larger amount of reagents, which is less economical.
All of the reaction components can be mixed in together in a 0.5-mL PCR tube in any sequence except for the DNA polymerase, which should be added last.
It is recommended to mix all the components right before PCR cycling. Although it is not necessary to set up the PCR on ice, some published protocols recommend it.
The common volume of a PCR is 10,25, 50, or 100μL.
Although larger volumes are easier to pipet, they also use up a larger amount of reagents, which is less economical.
All of the reaction components can be mixed in together in a 0.5-mL PCR tube in any sequence except for the DNA polymerase, which should be added last.
It is recommended to mix all the components right before PCR cycling. Although it is not necessary to set up the PCR on ice, some published protocols recommend it.
1-500 ng/μL template DNA (stander)
50μM forward PCR primer
50μM reverse PCR primer
25mM MgCl2
2.5mM dNTPs
10× PCR buffer
5 U/μL Taq DNA polymerase
C1V1=C2V2
C1= original concentration (stock).
V1= original volume
C2= required concentration
V2= PCR reaction volume.

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