I'm about to start my research internship as an undergraduate sophomore, so this video was helpful. Thank you!
@emishaemi53562 жыл бұрын
Tnq for explaining and demonstrating the pcr method very specifically. It really helped me so much. Im a biotech student doing my second year but due to covid i never went to my campus even once. I was lacking practical knowledge and worried about it so much but this video was really helpful
@sccm1003 жыл бұрын
Loved this video. Just watched it an hour before my biotech lab.
@renukadwarampudi24292 жыл бұрын
Wow such a great n clear explanation with visualization. Thank you. It will be helpful for practical work.
@qwertito79362 жыл бұрын
This is helping me study for my finals but I couldn't help but notice that the tip case was open the whole time, thats a little risky
@nutsackmania2 жыл бұрын
Gotta keep 'er movin
@longlosttreasonlearnerlast35513 жыл бұрын
helping me push my grades up thnx
@Tasha-lz1ic2 жыл бұрын
Gotta love the energy in this video, man
@mondaymiracle439 Жыл бұрын
I found this extremely useful. It's so simplified. Thank you for sharing.
@georginafavour97512 жыл бұрын
I love this video. I just subscribed to your channel. Very informative and interesting.
@manithsiriwardana8724 Жыл бұрын
This video was very helpful!
@meugeniaaso Жыл бұрын
Thank you for this amazing video. Excellent explanation and visualization.
@johnmcconnell7961 Жыл бұрын
Phenomenal explanations and techniques.
@kasparsstraut22473 ай бұрын
This is Gold! Thank you!!!!
@LabXchange3 ай бұрын
Glad it was helpful!
@onyeaborphilip3898 Жыл бұрын
Hi guys. There's something I don't understand. Where did he put the DNA sample to be amplified?
@tony232cool2 жыл бұрын
so what about the results? what did you learn from this work
@quantummandavid2 жыл бұрын
Thank you so much for sharing this video. I am curious do you think if you were processing multiple PCR test at the same time that a viral particles can travel from one specimen sample to another or is it obviously best practice to sanitize everything before you process another sample. What do you think the industry is doing nowadays to prevent contamination from one container to another when they’re doing mass testing for covid 19?
@nutsackmania2 жыл бұрын
Huge amounts of one-time-use plastic brotha.
@Jmurse892 жыл бұрын
Maybe I'm misunderstanding your question but each tube had a locking plastic lid so I can't imagine any particles being free in the machine.
@quantummandavid2 жыл бұрын
@Jeremy Marsh I could have posed the question with an example. let's say person A has viral shedding occurring. They sneezed in their car several times before arriving to the testing facility. They pull up roll down the window with their air in car spreading those viral particles all over the place. The person who takes the sample now has the virual particles all over their person because they reached their arm in the car of individual A. Throughout the day the person who takes the sample may not knowingly be dropping the particles off their ppe into sample containers. @ Jeremy Marsh do you know if they replace their PPE including the gown they wear after taking every sample from a person in a car? If I got tested I may have or not noticed they did not do that Change their gowns after taking someone's sample. I almost felt like for all I know this person taking my sample could be dropping the viral particles in my car. I am ignorant of how all this works but from my understanding, the viral particles from people shedding comes out in the billions, and in a car with the heater cranked it's just flying all over the car when people shedding cough or sneeze in their cars before coming to get tested. This is one of many examples of how containers or even bins containing samples can get contaminated.
@Jmurse892 жыл бұрын
@@quantummandavid I would think if a larger than normal positive rate was traced to a certain testing site, they would have to look at the collection technique. Furthermore, the sites I've been to has the patient self-collect the sample and seal the test tube themselves, so the probability of contamination is negligible if not nonexistent. I'm an ER nurse and have collected and sent several hundreds if not thousands of tests in the past 2+ years, and the negative rate is more than I might expect, and trust me, I go allll the way into the nose!
@Xen2392 жыл бұрын
@David espinoza If I’m understanding your question, I believe that after a pcr, one would run a gel electrophoresis- which includes a negative test tube for checking contamination besides reassurance that there’s indeed genetic material in samples. I hope this helps
@DiemNguyen-gg8gy Жыл бұрын
Thank you so much for a great video!❤❤❤
@casbee8857 Жыл бұрын
Wonderful explanation 🎉
@Hoxgene2 жыл бұрын
Initial denaturation was 5 minutes? were you analyzing eukaryotic genomic DNA??
@aliercemustun3525Ай бұрын
It's very useful, thanks👍
@devonfernando7273 жыл бұрын
Thank you, a very informative video 👍
@joyjohn74473 жыл бұрын
Herbs are the best, I got cured completely using DR RORPOPOR HERBAL on KZbin herbs supplements to defeat PCR • his medication is active....... YOU ARE THE BEST
@Bthna3 жыл бұрын
is it compulsory to start the pcr programm with the initial denaturation step (95/5min)?
@LabXchange3 жыл бұрын
Great question! No, holding this temperature for 5 min is probably not really compulsory- most of the DNA is probably denatured before 5 min. We often hold this first step for 5 min just to ensure that as much DNA is single-stranded, which should maximum how much PCR product we make after 30 cycles. But the length of time of this initial step is a variable we can change to optimize the yield of the reaction. The time duration of this step can depend on the other experimental conditions: the the template and primer DNA sequences; the type of enzyme being used; and the salt concentrations of buffer. Using 5 minutes often works, but we can change it to optimize the reaction if it doesn't work the first time. But the temperature is relatively compulsory. PCR requires the denaturation step (initial and otherwise) to be programmed at a high temperature, commonly between 94-98°C.
@Bthna3 жыл бұрын
@@LabXchange Very clear! Thank you for your time :)
@Zedmwiru.10 ай бұрын
So helpful, thank you.
@utkirjonshapulatov9803 жыл бұрын
GREAT. YOUR PRONOUNS VERY WELL AND I UNDERSTAND YOU
@dtrain1383 жыл бұрын
Where can I get one of these PCR machines? And how much do they cost?
@VOSTOK3225 Жыл бұрын
biorad
@sisiphosathula10302 жыл бұрын
Are you adding everything into one tube?
@super-hero82792 жыл бұрын
No
@ms.jutamatnonnoy19813 жыл бұрын
Wowww, very kind of you, it's make me clear about PCR Method
@virenderbhardwaj31372 жыл бұрын
One thing I don't understand as a high school student is when they'll design complementary primers aren't they already aware of the sequence of the DNA they are amplifying? because primer is complementary to the strands so when they'll design them they should know the sequence of that DNA right?
@farhansukarno52992 жыл бұрын
yes, they need to check genebank of what DNA sequence they want to amplify
@chocolatecharley992 жыл бұрын
Right so the whole point isn't to know what the sequence is (that would be sequencing). It's to make many copies for further testing.
@mahbubmorshed44882 жыл бұрын
just lovely ..from Bangladesh
@zahraafakih70202 жыл бұрын
Could you show us how to perform cell culture of human cells
@EZINNEUCHECHIEKWUJURU Жыл бұрын
Good job.
@aousshaheen323911 ай бұрын
Thanks
@holysheet3502 жыл бұрын
it really helps me. tysm
@kiyanazare5668 Жыл бұрын
That was great thank you
@hamedsarveghadi7552 Жыл бұрын
so great 🙏
@hebahalfrajeen850 Жыл бұрын
thank you so much
@LarsLarsen77 Жыл бұрын
You keep opening the vials from the ice bath with one hand, you're contaminating them all.
@mariaagustin5368 Жыл бұрын
thank u so much
@shaimaboosary14133 жыл бұрын
Primers are synthetic RNA molecules right??? Not DNA?
@LabXchange3 жыл бұрын
Such a good question. In the cell, yes, you are totally right: primers are made as short RNA molecules. However, when we do it in a test tube, we typically use DNA primers (which have several benefits, such as increased stability.)
@shafserious28052 жыл бұрын
How did you pick up 1ng!!! Thats 0.001ug!
@timothyb.fasoye13492 жыл бұрын
Great
@omarijoseph31892 жыл бұрын
so what does all this mean in ENGLISH !!
@shafserious28052 жыл бұрын
🤣🤣
@Stronger.1193 жыл бұрын
❤️❤️❤️❤️
@shafserious28052 жыл бұрын
PCR was not developes for diagnosis and should never be used for this