Plaque Assay for Influenza Virus

( www.abnova.com ) - The plaque assay is a widely used approach for purifying a clonal population of virus and determining the viral titers (the lowest concentration of virus that results in infection). This video shows the procedure of plaque assay for influenza virus. More videos at Abnova www.abnova.com

[@middlevoids]

Nice and clear. Thanks!

[@shanyuxi]

always use filtered tips!

[@fups1]

Great music and even better video! thank you!

[@paulabenitezbolivar5545]

great video! thanks!

[@ithagag1751]

Very helpful video

[@lyvian0321]

Hi tq so much for this video. Very helpful. Anyway, I wonder where is your source or more precisely how should I cite this method in my paper later?

[@omaradenis1196]

I would like to inquire whether the medium mixed with 3% agarose is containing FBS or not.

[@ThuNguyen-jj8de]

it not contain FBS but i think it contain TPCK for virus infection

[@adarshguptak]

Yes, the growth medium with agar should have FBS. Avoid FBS only in virus dilutions as it may affect cell adsorption.

[@melissarobinson9365]

Great video.

[@Sh.zaeemi]

Fantastic

[@HO-YTchannel]

But why 1080 ul and 120 ul and not just 1000 ul and 100 ul ?

[@maureen2888]

I know right? I was wondering the same. Why complicate things

[@bussayaratmaikhunthod2251]

Just to make sure you have enough virus solution to infect the cells. In this video they use 500ul for each well and they did 2 rep(wells) per dilution of virus. So if you prepare exactly 1000uL, with the general error of pipetting you surely don't have enough virus stock for 2 wells.

[@ThuNguyen-jj8de]

they have 2 plates for the duplicate experiment so they need at least 1000ul virus solution and the dilution ratio should be 10-time dilution so it is 100 virus 900ul (total 1000ul) therefore with their experiment, they want to make sure they have enough virus solution with 1200ul in total

[@ThuNguyen-jj8de]

thank you so much

[@dianindierahmalia2301]

What is a cell solution? A sample or not?

[@adrianwoodlock8433]

Can someone help me understand what this experiment is showing

[@ThuNguyen-jj8de]

virus titer

[@herbeyoswaldo]

don't you use trypsin to infect?

[@紀博文-i8l]

nice

[@asoro_1632]

Pozdrowienia z uzetu

[@kurniawahyu]

Is it work with c6/36? except for 37。C degree, (c6/36 requiring 27。C instead), all the step would be same? I am looking forward to count my virus titer using C6/36 Thank you

[@bhuvanroyal5909]

Could you explain how we get Dilution factor.

[@UmerAli-lr9ll]

Depends on whether your virus is cytolytic on C636 or not. If you want to just do the growth/replication kinetics of your virus on C636, you can use the infected supernatant from C636 at different time points and titrate it on more susceptible cell lines like Vero E6 or CV1 cells. Some arboviruses despite giving good yield from insect lines wont cause much cytopathic effects.Good luck!