Cell Culture (Attached Cell)

[@TheBeesKnees1993]

Thank you, this video is perfect! I had training on this yesterday but with so many steps, it's tricky to remember everything. I'm currently studying for a Masters degree in Infectious Diseases and I have to use this technique for my Thesis project. Thanks again :)

[@mywaveinwoodswoodperfumes]

***** for me no more humiliating from my supervisor, due to repeated asking about the technique :)

[@JESUSSavedMefromDeath]

Did you obtain your Master's?

[@TheBeesKnees1993]

@@JESUSSavedMefromDeath I did indeed ☺️ Studying towards the next qualification now. It feels like it never ends but hopefully it pays off in the future🤞🏻

[@JESUSSavedMefromDeath]

@@TheBeesKnees1993 Congratulations! I am happy for you and it will definetely pay off! Good Luck in everything!!! You're an inspiration!

[@TheBeesKnees1993]

@@JESUSSavedMefromDeath Aww thanks, that's really sweet ☺️ I'm not doing anything amazing but hopefully I can be a small bit useful to someone. I'm sure you are doing great too.

[@funny11744]

I am interested in finding out if the smallest surface ( that with volume signs) of the flask is treated for cell culture.

[@jacobswolfgirl112]

The music is so soothing lol

[@creativebioarray5879]

Hematopoietic stem cell harvest apheresis is a special form of leukapheresis used to collect cells from the peripheral blood.

[@supremelana9903]

Hello. I have a practical work with MDA-MB-231 cells to perform, could you tell me for how long do you incubate them at 2:10 ? Thanks in advance

[@funny11744]

What is the composition of the liquid used for discharge cells that are not used anymore in the process ?

[@benguemichele4963]

Thank youuu! this video is just perfect! Tout est clair pour moi maintenant en culture cellulaire

[@priyavishnoi9073]

Thanks for the video. It taught me perfectly.

[@minalthacker4324]

Thanks for the video, it was really helpful.

[@PRITAM0000]

Nice cell culture

[@vandanagupta8919]

excellent video

[@salilpatel9631]

By this method active viruses are produced or inactive viruses ???

[@ganghit7278]

이야 이젠 영어로 뜨네... 11년 전꺼잖아

[@ellocoassis]

Very informative this video. Thank you!

[@MrEphratah]

Thanks a lot. This is great!

[@akshaykulkarni1756]

What type of centrifuge was used in this video?

[@أحمدنعيمعبدهنيدي-ض3ض]

Any one types, its not important but, remember that the suspension cells must be centrifuged at 200 rotation per min (rpm) for 5 min. With my ❤ for you.

[@albert2290]

thank to this video, I understand cell culture beter

[@adrianwoodlock8433]

When are controls done with cell culture

[@kosheeka]

Controls are essential in cell culture experiments to ensure the validity and reliability of your results. Here are the key times when controls should be incorporated: • Before Experiment Initiation - Cell Line Authentication - Mycoplasma Testing - Media and Reagent Quality Control • During Experiment Setup - Positive and Negative Controls - Treatment Controls • Throughout the Experiment - Monitoring Cell Growth and Viability - Contamination Checks

[@amora303]

great job i wonna ask a question what is the function of EDTA??

[@TheCervelas]

in case someone wonder that too , dmso is basically antifrost

[@NewWesternFront]

@@TheCervelas EDTA

[@NetrapalSinghShekhawat]

Nice work...

[@tithteeyarattanachotapsawa3175]

Is it the same if the medium used is RPMI?

[@dr.mithunsarangdhar4099]

Yes

[@kosheeka]

It is crucial to understand that the protocol differs based on the cells and not on the type of media we are using.

[@binbinky78]

very helpful

[@shinuvet]

nice video, thanks

[@yasmeenadel4181]

Perfect ❤️

[@anwang5208]

Big thump up to you!

[@mukhamadefendi518]

App

[@vinaykumar-xp3uz]

great

[@TheCervelas]

perfect

[@muziliujin]

brriliant!!

[@ot8479]

Sorry the images are not clear and the music on a scientific video could be skipped and replaced by clear guidance.

[@arberselmani5049]

Perfect 👌🏻