Polymerase Chain Reaction (PCR) Live demonstration. Practical process for PCR

[@sumanpatra7380]

This is a fantastic video for learning and getting knowledge..For the reson of COVID I am unable to learn practical techniques and handle of machines and apparatus..and in rural areas Universities there is no advance lab present..Thank you for this amazing demonstration..Plz sir and mam ..make many more technical demonstration of life science practical..There shall be I remain grateful..🙏

[@ampliconsofbiotech]

Thank you for the appreciation. Stay connected with us as we are trying our best to cover all the basic and advance practical techniques.

[@sumanpatra7380]

@@ampliconsofbiotech Thank you so much 🙏🙏

[@hudanoor6009]

After travelling through so many channels finally i got the right one..thanks for uploading such videob

[@ampliconsofbiotech]

👍we will upload more pcr related videos on our channel, make sure to subscribe for the updates 😊 thank you!

[@absharalam9114]

you shouldn't have added the background music, it's very distracting.

[@ampliconsofbiotech]

Ok from next time I will not add

[@unseen4721]

💯💯

[@debeshpramanik8435]

♤♧100%

[@rohitsaha371]

No it's fine... For video addition to gain some interest ... Maybe the volume should be lower ..that's it

[@freefirelovers.9730]

IT'S simple And clear vedio. THanks 👍🏻

[@aqsamansuri5927]

Awesome U explaining in fabulous way

[@Sanahfatima1]

Well explained. May all your wwishes come true.

[@learnthefacts9068]

This is much needed and bringing best out of demonstration and will helps each student individually for instruments and handling. Thanks you Amplicons of biotech.😍

[@ampliconsofbiotech]

Most welcome @rajeshtamatta9068 we are happy to see that you are satisfied with our video and it helped you understand pcr with more clarity in easiest way. 😊

[@learnthefacts9068]

@@ampliconsofbiotech yes in most clearly and effective way again thanks

[@shahbanfarooq7721]

​@@learnthefacts9068Lllll4rlll4rlllll

[@conceptualbiology6423]

appreciate your effort because I found first time like this video on youtube keep it up.

[@ampliconsofbiotech]

Thank you so much for appreciation

[@shubham7098]

Thank you . Crystal clear demonstration.

[@ampliconsofbiotech]

Happy to help

[@muhammaddanishtoor9642]

guys, you are doing very super work. Really appreciate it. Keep it up.

[@ampliconsofbiotech]

Thank you for your appreciation 😊

[@payalsaini1212]

It's a best way for learning....tnx

[@ampliconsofbiotech]

Most welcome

[@poojatiwari6479]

You're doing awesome things ❤ it is really helpful for many students (like me thanks a lot 😇

[@ampliconsofbiotech]

Thank you 👍

[@manjubisht2550]

Very informative and useful. Kindly continue the series of video and upload as much as possible all biotech lab experiments

[@ampliconsofbiotech]

Sure I will

[@manishchavhan252]

Great information Sir and Mam👏

[@ars007bond]

Loved it, thank you so so much for making this video🎉 .

[@simraulla6095]

U both of u did a great job ....it help me alot ... thank you...

[@ampliconsofbiotech]

@simra it's our pleasure to hear that our video has helped you understanding PCR. Stay connected to the channel for more such videos, and let us know if we can help you and others in any topic related to Molecular biology, biotechnology, etc

[@japanraval9995]

Great way of explaining

[@jyoti2446]

Best ....best.... video on PCR.... thank you very much ❤ I have some questions Regarding PCR 1) 1 Ul kya hai.... 1) 5U aur 1U taq ki quantity ko kaise nikal kisi rxn main. 3) Kis company ka "Taq polymerase" use karna hai, aur uski kitni quantity milti hai aur price bhi mention kar dijiyega. Please 🙏😌🙏

[@ampliconsofbiotech]

Thank you, we are happy that you liked our video. Your questions will be answered asap.

[@vrushalisavvasher2065]

Very informative.. Fine explanation.. Thank you!!

[@imtyazmahar378]

❤

[@pandurangkanhe1392]

Thank you sir for this important information of PCR 🙏🙏🙏

[@ampliconsofbiotech]

It's our pleasure @pandurang let us know what else we can help you with, we are open to suggestions for more topics in biotechnology experiments and concepts.

[@theshifzz-u6r]

Very helpful video sir. Thankuu so much

[@ampliconsofbiotech]

Most welcome

[@themusicworld8064]

Thank you sir and mam for explaining each and every part ❤

[@ampliconsofbiotech]

Thank you for your appreciation

[@mmukundan5758]

So much useful 💯

[@swetayadav28003]

Amazing video tysm.. 🔥🔥💯💯

[@ampliconsofbiotech]

👍

[@surbhichaudhary3695]

Insightful.. 🤩

[@ampliconsofbiotech]

Thank you 😊

[@areenasiddiqa4239]

Very helpful..

[@SaumyaSharma123]

Sir i have some queries. I made a gel of 1.8 % and my DNA ran faster then the ladder!!! I used primer of 218 bp and ladder of 50kb. My DNA bands crossed the 50kb band 😓😓😓.. (O.D was 1.6) annealing temp 58

[@ampliconsofbiotech]

If you are loading only DNA then go with 0.8% gel, and if it's PCR product try loading 100bp ladder because your product size is 218bp, in 2% or 3.5% gel (volt 90-120v)

[@SaumyaSharma123]

@@ampliconsofbiotech and for how much time??

[@SaumyaSharma123]

@@ampliconsofbiotech sir , I’m talking about PCR product. I even dont know how to make a reaction mixture calculation of DNA ( like how much DNA should I take from my extracted total DNA ) I just used one paper and followed it might be that’s y I failed in same. I need to contact u. Plz help

[@ampliconsofbiotech]

After loading samples you can check for amplification one hour later under uv light torch, on 3.5% gel the product of 218 bp will take 2 hours to separate

[@ampliconsofbiotech]

You can contact us at 9834128616 then according to your DNA, primer and reaction we can finalize a protocol

[@rajukatkade8407]

very informative and highly knowledgeable topic. Thank you meghraj Sir ❤️

[@ampliconsofbiotech]

Thank you Dr. Raj. Keep supporting 👍🏻

[@rajukatkade8407]

@@ampliconsofbiotech ❤️

[@Bachokikahanias]

Bro good informative video

[@Finalthings]

Just wow explanation

[@sapanagold]

Thankyou very much sir and maam 😊

[@Boo_eats]

thanks it was very helpful

[@nihallittlehero9556]

Thank you.very informative.

[@ampliconsofbiotech]

🙏

[@alishaytariq9118]

very good explanation ✔

[@ampliconsofbiotech]

Thank you

[@MuhammadArslan-lf9eb]

Thank you sir

[@AhsanJahangir-h1g]

love from Pakistan !❤

[@pdnebche1054]

Fantastic video

[@ampliconsofbiotech]

Thank you so much

[@pdnebche1054]

@@ampliconsofbiotech thnk u sir

[@jamthakur7093]

Very good helpful 🙏

[@ampliconsofbiotech]

Thank you

[@ayazali4089]

Fantasticks 👍🏻

[@ampliconsofbiotech]

Thank you

[@b-08-bhoomi43]

Gel electrophoresis me aage ki process kese karte he ?

[@meghrajchavhan55]

kzbin.info/www/bejne/jmrMl5V7i6pmqacsi=3fXv08dot55W5a-g watch this gel electrophoresis video.

[@ampliconsofbiotech]

Click on the link below and watch our video on electrophoresis

[@Exploregermanywithzeba]

Great efforts😊

[@ampliconsofbiotech]

Thank you

[@shutijakharade367]

Very Helpful! 😊👍

[@ampliconsofbiotech]

Thank you

[@mohammadhassanramezanzadeh6278]

Thanks a lot.

[@tejasvi8851]

Very nice explains brother 😁😊

[@ampliconsofbiotech]

Thank you

[@CharuChoudhary-i7q]

Thanku so much bhai ..

[@AmitThakur-py4te]

Great❤❤❤❤

[@abdurrasheed6477]

Just great but, kindly also cover the gel doc system for the visualization of DNA. Thank u.

[@ampliconsofbiotech]

Yes for sure.

[@riazmohammad-e9f]

❤❤❤

[@Bachokikahanias]

Good job bro

[@ampliconsofbiotech]

Thank you

[@tauseefwani9636]

Excellent video Sir, rise and shine

[@ampliconsofbiotech]

Thank you 😊

[@shwetadinde3922]

Good demonstration😊

[@ampliconsofbiotech]

Thanks

[@najeebullah6776]

Thank u both

[@mohdshirazsheikh3741]

In Pcr primer bind to target region of dna and with Polymerase enzyme further step start my question is . in template dna some portion where primer not bind when reaction start. what about that portion where primer not bind .how this portion multiply

[@ampliconsofbiotech]

It won't, primers are specific to a particular region and will bind to the target site of your template.

[@susmitaparida4565]

Thank you so much for the video. 🧬 sir can please show the result part through gel electrophoresis after a pcr.

[@ampliconsofbiotech]

We have already uploaded a gel electrophoresis video in general context, for these results we will soon upload 96 wells gel assembly video. To stay notified press the bell icon and make sure to subscribe. Thank you

[@Simp247]

Thanks

[@ampliconsofbiotech]

.

[@mabdulm412]

Thanks to both of you. I gained some knowledge, but not fully. I think that to fully understand, I need to practice it myself. Please check my comment for mistakes, fix them, and let me know.

[@ampliconsofbiotech]

We are happy to hear that you learned from our video, to make it more stronger you need to practice with a few samples, multiple times. Best luck

[@dmadhav4867]

Why did we change the temperature to 56degreescentigrade .

[@bhavikajain4233]

Dna extraction

[@bhavikajain4233]

High tem.m denature..

[@ampliconsofbiotech]

To get to the next step of annealing we keep temp 55-60 depending on the primers used.

[@priyankaverma3572]

Nice

[@cutepaapa9153]

Sir how the students can perform PCR training course

[@ampliconsofbiotech]

There are a lot of workshops by private companies, and also training programs for students at multiple ICAR and ICMR institutes, you can visit particular websites and search for training programs. You can apply to IARI student internship of 3 months

[@ampliconsofbiotech]

If you are unable to find it, let us know

[@cutepaapa9153]

@@ampliconsofbiotechthank you for yours responds sir , and how to find this internship in Chennai kindly help me sir

[@shilpagoyal3921]

Why do we need to have a number of wells in this way? Why not a single big size chamber to run PCR?

[@madanikanagaraj]

Hello sir inorder to join in laboratory (like you ..) which degree we should complete bsc biotechnology or btech biotechnology ..

[@ampliconsofbiotech]

You can go with any of these but further you need to do atleast a master's degree after graduation

[@vaishnavichavhan4236]

👍🏻👍🏻

[@muhammadpervaiz8347]

Please make video on real time pcr , cDNA ,cDNA dilution, optimisation and then qpcr. Plzzz it is a request

[@ampliconsofbiotech]

Yes we will make a video and upload it soon

[@maheshchougale3079]

Any bubbles formation in final sample and we insert into PCR machine then variation in results

[@ampliconsofbiotech]

Bubbles don't create any problem, just a 30 second spin to the vials will sort everything

[@manishagupta6543]

Yes please make one kn rtpcr as well

[@ampliconsofbiotech]

Yes we will prepare another video

[@nandinichaudhary2962]

Thank you guy's :)

[@ampliconsofbiotech]

👍

[@nehanagrale9994]

Sir I want to know the method about origin determination

[@ampliconsofbiotech]

Please elaborate

[@soumyanigam9657]

Thanku sir can u pls explain plate setup or template for conventional pcr

[@ampliconsofbiotech]

Yes we will try to make a separate video on conventional PCR

[@HashtagAnik]

Thank you bro❤️

[@ampliconsofbiotech]

👍

[@PoojaSingh-cq9mc]

Kya RT PCR result ko -20 degree par result ko store kar skte h?

[@ampliconsofbiotech]

Yes we can store it.

[@AyanshAyansh-sy5rr]

How to design primer... Can you make a video on it

[@M_5787]

Thankyoy so much sir 😊

[@ampliconsofbiotech]

👍

[@MeghaNikum]

thank you

[@ampliconsofbiotech]

👍

[@ZulqarnainHadir-e8x]

Also make video on gel electrophoresis

[@ampliconsofbiotech]

Already on channel, please visit videos section to watch

[@snehaldesai4423]

What is 30 sec step between denaturation and annealing

[@ampliconsofbiotech]

There are overall 5 steps, Initial denaturation for 5 min then the cycle starts with (denaturation - annealing - extension) last final extension for 10 min

[@hibbashah4546]

Can we spin in centrifuge machine

[@ampliconsofbiotech]

Yes

[@MohdJaved-gr7mh]

Sir iski quantity kaise decide karenge kaunsa component kitne quantity mein add karna hai ????

[@nehartandcraft]

Plz tell the quantity add kitnr kiye h

[@emanmagdy1999]

I have a real time pcr pioneer excicyler 96 ,how can I use it . I don't know ،

[@ampliconsofbiotech]

Just try following the manual. All machines are near about same, you sure will find out how to operate it.

[@santoshinaik4612]

Thank you so much!

[@ampliconsofbiotech]

👍

[@rekhagautam2905]

Ilongetion ,anniling , pcr Done, k bad weight Kitna bdh jata h. Comming product ka

[@m.talhanadeem3172]

Bhai pcr thermal cycler mn eppendorf tube ka temp kitna hota hy.

[@ampliconsofbiotech]

Tube ka koe temperature nahi hota hai, machine ka temperature jyata hai 110 degrees tak jo tube ki capacity me hota hai isliye tube melt nahi hoti. Sometimes if low quality plastic wares are used then tubes might melt in pcr

[@m.talhanadeem3172]

Ok thank you bhai v.good video

[@prajwalgosavi2001]

Can heparin be used for pcr qualitative? Or what will happen with result?

[@ampliconsofbiotech]

Hairpin PCR can be a useful tool for qualitative analysis of RNA molecules, but careful optimization and validation of the assay are necessary to ensure reliable results.

[@prajwalgosavi2001]

@@ampliconsofbiotech so does that mean results may be reliable ?

[@maagivy7925]

Fantastic video sir. I have a doubt, I am using applied biosystems gradient PCR machine. When the cycle is completed it shows time remaining as 00. 0 hours at that time can I press the pause/ stop button?.

[@ampliconsofbiotech]

Yes, once the program is ended and temperature goes to 4 degrees you can click on stop/ pause button

[@maagivy7925]

@@ampliconsofbiotech Thanks for the reply.

[@Bachokikahanias]

Continue other video

[@ampliconsofbiotech]

Yes sure

[@aartipatel2759]

Sir intrest of DNA kon se step Mai add karinge

[@ampliconsofbiotech]

If you are adding individual components, you can add dna at starting step or the last step.

[@srushtipund7321]

👍

[@ampliconsofbiotech]

Thank you

[@shubham7098]

Sir how to identify microorganism using sequencing?

[@ampliconsofbiotech]

Metagenomics is the technique for identification, it would be more helpful to answer if you could be more specific about the question.

[@shubham7098]

@@ampliconsofbiotech identification of using Sanger sequencing. Sanger sequencing of internal and external microbial cultures.

[@ampliconsofbiotech]

See the principle is when a pure PCR product of the 16S gene is obtained, sequenced, and aligned against bacterial/microbial DNA data base, then the microorganism can be identified. Now what exactly are the steps involved and how it's practically done is little bit complicated to explain in the comments

[@shubham7098]

@@ampliconsofbiotech OK . Thanks a lot

[@rajalaxmi7718]

Plz suggest how to make primer.

[@ampliconsofbiotech]

You how to design primer, or you can use available ones from different databases

[@maheshchougale3079]

Needful information for me

[@ampliconsofbiotech]

👍

[@rgalizx411]

Dear real time or thermal cycler Mae Kia faraq Hy ?? Kia thermal cycler Mae channel nhi hotae kiaa ??

[@ampliconsofbiotech]

Thermal cycler is just name for PCR

[@hariomsinghal5868]

Sir ham kese pta krte hain ki hame konse part of dna ki hi copies banani hai

[@ampliconsofbiotech]

By using region/trait specific markers, organisms genomes are available on data bases. Bioinformatics tools ki help se marker design karte hai aur fir usi region k sath wo marker/primer attach hokar amplify karta hai region ko.

[@hariomsinghal5868]

@@ampliconsofbiotech sir jab dna ke specific part ki bahut sari copies ban jati Hain to unke sath me wo extra dna bhi to machine me rhta hoga fir ham only specific part ko kese separate krte hain

[@DoD193]

Q.1what is nucleic acid prob technology? Q.2what is nucleic acid topology?

[@ampliconsofbiotech]

Probe- the use of DNA or RNA probes to detect particular gene sequences. Topology- how the two complementary single strands are intertwined in Watson Crick model

[@CrazyZarah]

Sir can you make a vedio of reporting a hiv pcr

[@ampliconsofbiotech]

We work on plants

[@nehartandcraft]

Pipatt kitna use kiya. Scale qo. Sb. Btaye n

[@ampliconsofbiotech]

What exactly do you want to know please clarify the question, we will be happy to answer to all your queries

[@nehartandcraft]

@@ampliconsofbiotech sir i dont. Know. The. Pcr. Reaction. Practical. How. Did. U perform. Yhis. Did not understnd. Plz exaplin. With. Out. Outside. Distraction or soutnd. Theory. Explain. With pracrucal. M msc. Zoology. Du. Dept. Dept. 1. Sem.

[@nehartandcraft]

Add. Also. All pgm. Practical. Of. Msc. 1 sem. Now. M. In. In. Du. So. Did not understnd.

[@ayazali4089]

I am from pakistan. Main ny app ko subscribe karleya.

[@ampliconsofbiotech]

Thank you 👍

[@Agri_Movie-441]

Extract kasy Kiya DNA?

[@ampliconsofbiotech]

kzbin.info/www/bejne/kH7TdX2Vr9l-kJo Watch this video to know how we isolated DNA?

[@MayankTripathi-rl6gj]

Sir please tell us career options after Btech Biotechnology,or after qualifying GATE.. What are the job opportunities after Btech Biotechnology... please sir

[@shivanandyadav2413]

94...96 do time Kyo lete h.... Means sequence me

[@ampliconsofbiotech]

Samples pe depend karta hai jaise kisi me standard is 95 but can vary from samples, kisi ka denaturation 94 pe hota hai to kisi ka 95

[@shivanandyadav2413]

@@ampliconsofbiotech Lekin.... Aapne dono set kiya h.... Thoda bta dijiye..... Waise bahut bahut Dhanyawad...... Aapne reply kiya 🙏

[@ampliconsofbiotech]

Ok I got your question. Their are two steps for the reaction, first is denaturation at 95 or 94 for 4 to 5 minutes then comes the cycle of pcr (denaturation, annealing, extension) and at last final extension

[@shivanandyadav2413]

@@ampliconsofbiotech thank you sir🙏

[@everythingsimple3529]

rt pcr pe vdo banaiye practical

[@ampliconsofbiotech]

Ok