How to determine the Protein Concentration with the Bradford Assay

Hey Friends,
protein quantification techniques are used to determine the total concentration of protein in a solution. One among many colorimetric assays for this is the Bradford Assay developed by Marion Bradford. The assay is easy to perform and delivers results in a short time. The Bradford test is based on Coomassie Brilliant Blue G250, a dye which binds to proteins in an acidic environment.
References:
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72(1-2), 248-254. www.sciencedirect.com/science...
www.bio-rad.com/featured/en/b...
www.thermofisher.com/de/de/ho...
Thanks!
Henrik
Time Codes:
0:00 Introduction
1:04 Coomassie Brilliant Blue
1:44 Protein Concentration Determination
2:58 Advantages/Disadvantages
4:02 Outro

[@lilachgavish6751]

Thank you very much. I send this clear and simple explanation to all my students.

[@victorpham4221]

Thank you, this is a great summary!

[@mak3867]

Huge compliment for your videos Henrik! I would like to see some videos about RIP (RNA IP) and CLIP.

[@zohasadqat]

Well explained and to the point!

[@javieramacarenao.c778]

Thank you so much for this, it was very useful

[@migueltrujillo171]

a great video!!!! "keep it simple" is the rule :)

[@zunairaiqbal6810]

Very well explanation

[@mansisahare9943]

It's really easy and understanding video

[@samanthasaboia6557]

Thank youuu so much for this 🙏🏼🙏🏼🙏🏼💙💙💙💙💙

[@b36453]

Thank you for this

[@lojainashour]

Great job👌

[@orangecatheart]

thank you!! very useful

[@user-mm2zu1gs8c]

감사합니다! MO를 이용해서 색이 바뀌는 이유도 설명해주셨음 더 좋았을 것 같네요.

[@elisasaldivar8564]

awesome video

[@frebart367]

thank u i needed this

[@bioscienceswithshahtareenswati]

Very helpful 👌

[@sam26045]

Incredible

[@nbkhan4537]

Well explained

[@ricardoferreiravieira4258]

Amazing!

[@clemencebelbeoch6333]

Thanks!

[@chiaramurena2858]

amaziiing ! Thank u! :)

[@Luke55367]

Hello. In so many articles i read that coomassie blue transfers a free electron to the protein and this causes denaturation. Can anyone pls explain where this free electron comes from?

[@humi191]

Thank youuu!

[@user-ri6lr2jy2k]

ガチ助かる。 日本の薬学部生です。 英語の勉強も頑張ります！

[@henrikslab]

どうもありがとう

[@abdulazizabdunasimov8978]

I bet you're a german speaker. Thanks for the video

[@gud_soup6991]

how do you account for any dilutions

[@der_aller_einziche]

danke Henrik

[@sadpineapple4123]

@darshan rabari velleicht Deutschland lol

[@joaopauloferreirarodrigues5576]

I can buy the Bradford detergent compatible

[@henrikslab]

Or you do the BCA test instead

[@joaopauloferreirarodrigues5576]

@@henrikslab BCA is Good, but the Bradford detergent compatible in 96 well plate os more quickly

[@emanazmat13037]

Thanks❤🇵🇰

[@crimetax9100]

bruder mach ma auf deutsch in kann kein englisch

[@henrikslab]

Bruder wenn du Bio studierst, dann lern Englisch :D