Purine and Pyrimidine Nucleotide Biosynthesis

Moof's Medical Biochemistry Video Course: moof-university.thinkific.com/...

[@ceejayygambino1734]

Great video! Just one question - when you say the part about how ATP can't be used to make ATP, aren't we ultimately doing that in the process of cellular respiration?

[@MoofUniversity]

Christina Johnson Good question! In cellular respiration, we initially use 2 ATP to get glycolysis going. Once the glucose is entirely oxidized, however, we gross 32-34 ATP and net 30-32 ATP. So, in that case, we invested 2 ATP, got 32-34 ATP back from it, and so we earned 30-32 ATP. An analogy would be like buying something for 2$ and selling it for $32-$34 and earning $30-$32. Not a bad deal; right? On the flip side, here, it's essentially a 1-for-1 deal, an equal trade. As shown in the video, 1 GTP molecule is used to produce 1 ATP molecule. So, you might think that the analogy would be like spending $1 to buy something and then selling it for $1. That seems totally useless; right? But that's not what's happening here. You're spending $1 to get something OTHER than $1 back. Maybe you're buying a bag of chips with the $1 or a soda or a pen or a notebook - whatever. The point is that it's a trade. You're giving away something of value for something of equal value, but the thing you're trading away is something you don't want/need, while the thing you're getting in return is something you do want/need. It's not an investment with hopes of greater return on what you invested. It's more like a purchase or exchange. I hope that helps and makes some sort of sense.

[@bonbonpony]

@@MoofUniversity What if you have none? :q

[@jetpatterson6427]

Shout out from a medical student- you guys have saved my biochem grade this semester! Thanks for all of the concise and high-yield videos :)

[@petyailieva4794]

Amazing! U put the complexity into simplicity,and give us what is the real thing to remember!!! Thank you for saving my head of overloading with this clear summary !!!

[@robertjbender]

Nice work. You're way more clear and concise than most university profs.

[@jsboricua13]

Just wanted to take the time to say THANK YOUUUU!!! You are the reason why I'm doing well on my Biochem II class. I'll definitely be re-watching all of these videos in med school. You have no idea how much you have helped me. THANK YOU SO MUCH.

[@youngkim4937]

actually a pretty good video. Sometimes, in the sea of information, it's hard to figure out what you have to know and don't, but this video pretty much sums of what 's important to know. Thumbs up man!

[@MoofUniversity]

2TH DDS Word up, dude! Thanks! I'm happy you liked it.

[@eiklinoh9402]

A great start for me to keep understanding these synthesis as I am a first year medical student from Malaysia :) Thank you so much :D

[@claireshin7239]

im a grad student trying to prestudy biochem... this is extremely useful, and you are an awesome lecturer. thanks for your work!

[@George24FTW]

ridiculous how good these videos are, man

[@Gilke500]

Excellent organization and delivery! Thank you for your efforts

[@abduseburjemal3492]

Great teacher! Thank you for your elegant explanations!

[@vicentejcogollo2740]

Excellent video and excellent explanation. Thanks, the video was really helpful.

[@leeborne1223]

Thanks ; ) I finally can understand the process now. It's simple and understandable. And not confusing ! From KL Malaysia ;)

[@MuzikManiak100]

I love you!! So amazing! I was going to drop out of school but then I watched this and my life made sense!!! THANK YOU SO MUCH!!!

[@MoofUniversity]

MuzikManiak100 Lol. Wow! Thank you for the kind words. I'm happy to have helped. I hope you didn't drop out of school, and I'm glad life makes sense now! :P

[@user-lc3ws2ry8f]

Thanks for the simple and precise explanation! What a lovely channel :-)

[@AngelikaNK]

Cheers Mahfoud! Truly helpful.

[@HusseinBerjaoui]

Great explanation. Medical student here, you just made my life a little bit easier. Still a complex pathway, but kinda easier.

[@akmazam2713]

I have been seeking that.Thanks a lot.

[@Maja-ek6si]

Great overview! Excellent job! :)

[@syeda6697]

Much easier to understand compared to my lecturer! Thank you!

[@abdulhaq5997]

Excellent lecture!!! Using it to supplement my medical biochem class. Also used it multiple numerous times for my undergraduate biochemistry class. Are you a med student or a doctor by any chance?

[@n.ischiadicus1412]

Thanks for subtitle! Great video.

[@jo-jr2dv]

very clear and concise explanations, thank you

[@jejefree5225]

Thank you for this useful video!!

[@sashanealand8315]

Thanks Moof for just saying what everything means

[@WazeAngel]

You have no ideaaa how useful your videos are ! I study in french, so I'm not really fluent in English! I still think you explain it better than my biochem teacher does ! Thanks a lot! (Wish me luck, my exam's soon :( )

[@MoofUniversity]

WazeAngel Haha! Awesome! I hope you did well on your exam! :D

[@wawagirl]

my medical biochem disabled ass is saved. thank you

[@amymiller4340]

with Purine Ribonucleotide Synthesis, how would you go about explaining the effect of stressors at equilibrium?

[@NOETICS..]

Thank You So Much Sir. Keep posting videos. Very helpful.

[@ranimmuhiedin3339]

thank you , this was helpful

[@mohammadhussain7678]

Hey man! Thank you so much for this video! I was a little confused on the process of GTP from IMP because I believe the path to making GTP is IMP (NAD+ and H2O) then XMP (ATP added) where it then makes GTP. I'm not sure if it's a shortcut using your method or if there are multiple ways, so I was wondering if you can clarify a bit on that. Overall, a great informative video!

[@namanhtu4938]

"You don't use what you want to make to make what you want to make" that sounds kinda cool

[@unrivaled37]

Excellent!

[@ptomy1000]

Why does only the β-anomer result for the synthesis of nucleotides from PRPP?

[@griot1]

Hey! not to sound like a know it all, but i think the enzyme to get PRPP is actually a synthetase and not a kinase. ribose-5-phosphate using PRPP synthetase + ATP yields AMP and PRPP

[@MoofUniversity]

***** Sweet. Which textbook is that, exactly?

[@MoofUniversity]

***** Sweet! Thanks! Sure thing! I'm happy to help, and thank you for the kind words!

[@maximillianhowardpowell232]

I know this is two years ago but he is actually right and you probably know it by now. It really depends on your reference book... some books will call it ribose-phosphate pyrophospho kinase 1 and some will call it by the easy name to remember PRPP Synthetase.

[@weino3]

you just saved my tiny little brain from imploding, thank you! :)

[@MoofUniversity]

Chian Wein Haha! Awesome! I'm happy to have helped. You're very welcome. :]

[@armendayoung2086]

very helpful...thanks

[@kxrosheart9613]

Does purine biosynthesis is an energy intensive pathway as compared to pyrimidine biosynthesis

[@ricardotorres8114]

Very good

[@hstudy8406]

Awesome

[@bonbonpony]

So the pyrimidines are made out of L-aspartate? What about the purines? What are they made of?

[@user-nc3uv7gn9f]

How does purine de novo synthesis differ from pyrimidine de-novo synthesis?

[@oOkramiOo]

What are the end products of dTMP catabolism?

[@atheistmetal1]

at what point are two Carbon atoms sequestered in this model?

[@surat8591]

Thank you !!!!!!

[@KAROLPAULA]

thank you very much!

[@MoofUniversity]

You're welcome very much!

[@penoid6044]

Thank you so much for this vid ! Hopefully i will pass biochemistry now! :) greetings from germany

[@MoofUniversity]

penoid You're very welcome! I hope you pass! :] Greetings from Sunny Southern California! Shout-out to Deutschland!

[@mehrabsadventures4318]

Your voice is two steps away from being the announcer from Half-life doors (the ones that say Access Denied)

[@kxrosheart9613]

How much ATP is required for synthesis of pyrimidine ? And purines !

[@dhessysusanto8749]

thank you very much.... this saves me for exam haha

[@cbor0085]

fantastic videos! just wanted to ask what program it is you're using please

[@MoofUniversity]

Claude Borg Thanks, dude! For drawing, I use Smoothdraw 4, which is like Paint but better, and it's free. For screen capture and recording, I use SnagIt, which had a free trial initially, and I ended up buying it for $50 if I remember correctly.

[@cbor0085]

Thanks man! :) just had my metabolism exam and I most likely passed because of your videos ;) Big Thanks!

[@MoofUniversity]

Claude Borg SWEET! That's excellent! I'm happy to have helped, dude! :]

[@WhyNot-si4pj]

9:40 The reaction catalyzed by (CPS II) enzyme involves ONLY one molecule of water , NOT 2 molecules to get a balanced equation !

[@acc3095]

Hi moof! I'm wondering since IMP to GMP hydrolyse ATP to AMP does it use 2 eq. Of ATP?

[@MoofUniversity]

For that process, yes. Sorry for my late response.

[@acc3095]

no probs gonna use ur vids to learn lipid metabolism now :D

[@MoofUniversity]

Anthony C Sick! Sounds awesome, dude! Happy studying! :]

[@nurhamizah.hamdan]

what is the precursors for pyrimidine? and there is another precursor for purine right?

[@osamakhojah6304]

Carbamoyl phosphate and aspartate. Just ribose 5 phosphate.

[@adenanguyen8089]

Isn't the enzyme that synthesizes PRPP from R5P PRPP synthetase?

[@sureshir]

The enzyme goes by two names. Ribose-5-phosphate pyrophosphokinase is aka PRPP synthetase

[@jowannarobert9814]

Thanks aloooot ^_^

[@pneumonoultramicroscopicsi4065]

sadly we study about those "many steps" which give IMP from PRPP :(

[@sparkysparkyboomman3066]

clearly this guy be wingin it.

[@TheAnimalssex]

good

[@brylanjacobs9139]

I think you've forgotten a double bond between a nitrogen and carbon in your hypoxanthine base in IMP. Youre very good though!

[@MoofUniversity]

Whoops! You're right! Good catch, and thanks for the kind words!

[@mikaillal4687]

Well, I just don't understand. How come we go from ATP --> AMP + ppi when we want GTP, but when we want ATP we only go from GTP --> GDP + pi?

[@1david12]

I guess because ATP---->AMP + PPi gives more energy to drive the reaction to form GTP and for GTP-->GDP + Pi it doesn't need to give as much energy to form ATP? Idk, just a guess... Hopefully someone corrects me if I'm wrong.

[@Duppen]

@@1david12 It is because you only need to make ADP and then you use ETC to phosphorylate the ADP into ATP

[@mikkelbakjensen2350]

It makes me feel better about myself when even the teacher can't pronounce the the substrates and products. Dyhydroorotate at 10:51

[@paulhetherington3854]

pro--desired a scroll-- Kaldean. Phos= light in Greek, phate-- thief of light-- artificial.

[@amynwa5827]

I don't understand what you're saying

[@gunnarleitner5010]

check out my boy full klip

[@Austin-yv7mj]

+Gunnar Leitner I checked him out! He's pretty good.

[@jihuang771]

lt looks like that l-asp structure is not correct.

[@MoofUniversity]

It's correct. The stereochemistry is not explicitly shown, but that's definitely Asp.

[@jihuang771]

sorry, look the wrong way for the alpha carbon

[@omarelmarsafy1313]

@ COVID 19

[@rose12382]

I actually want the steps !!!!!!!!!!!

[@HellaPointsBro]

sikkkk

[@paulhetherington3854]

PY'--Hello in Hawaiian. Damn fail! rib= grapple, in Kaldean. This crap, isn't langauge-- taught in, US or EU? It isn't license able.

[@lawyerdoctor]

You use ATP to make ATP.....glycolysis......just saying your statement is flawed that you don’t use the product to make the product.

[@bekastepanishvili11]

T K yeah, we know it, he is just trying to give us an easy explanation

[@1david12]

Umm, just a quick tip: If you're doing youtube lecture videos, please refrain from having the first frame already having pre-written notes, viewers will get lost as they are unable to follow and you do not grab their attention effectively... The moment I saw that I simply paused the video, came to comments to type this message, then leave to find another video...

[@november8289]

david archer are you twelve? Grow up.

[@1david12]

Novem Ber Shut up idiot. I am simply correcting him so he helps viewers better with other videos

[@pneumonoultramicroscopicsi4065]

+david archer if you completed the video, you'll understand that he is the best biochemistry tutor on youtube and he is helping me even in med school.

[@bfurl69]

Moof rocks

[@munyaradzimurapa6738]

david archer Why does your attention need to be "grabbed" This isn't a movie or a video game or something, its up to you if you want to focus or not.

[@mahela1993]

Thanks a lot. :D