Radioimmunoassay (RIA): Animated explanation

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[@magnun123456]

Sajid, is seriously, your explanation and visual methods was SO AMAZING, thanks a lot!!!!

[@dr.janpreetsingh5103]

I have seen many videos on this topic but no one explained like this! this is such a nice explanation!!😊

[@Wilina13]

I missed a question about it in Uworld for USMLE step 1, and their explanation could not help me understand the concept, and you did. Thank you so very much!

[@annag27]

Omg YOU EXPLAINED THIS SO WELL. I get it now.

[@irissha9675]

I finally understood this technique, you made it very clear. thank you so much !!!

[@Immss]

I'm very happy now, all my doubts on RIA are cleared, thank u so much sir..😊

[@zayler_]

Thank you so much ! Your explanation on this topic is phenomenal.

[@kliusd]

*slowly claps* Well presented. Thank you, Sir. You are a legend.

[@avanraws77]

Good job...perfect explanation...keep up the good work!! 👍

[@chandlerbing5437]

Thank you so much ,saved at least an 2to 3 hours of time and energy....

[@MikeBassil]

Simpel clear and video wasn't too long, thank you!

[@hugodesrosiers-plaisance3156]

Very clear explanation! Thank you!

[@sajidmicrobiology]

Most welcome

[@ArvindSingh-jt6sg]

Wow !.. finally got it , amazing skills to aproach the topic 👏👏

[@sagorika5552]

I couldn't understand clearly in my class and was very tensed about it cause my exam is on thrusday.But your explanation gave me relief.very effective video......

[@Mzee2t]

Thanks a lot. This has helped a lot with my physiology course

[@IllusionsJunkie]

Thank you, this helped a lot! Excellent work! I'm studying in german, but I could still understand everything very well (:

[@sajidmicrobiology]

Most wecome brother

[@arvindhkumar819]

Crystal clear explanation. Tysm!!

[@uroojkhan6555]

Best video on KZbin that cleared all concepts waoooo... Urdu version b bnaya kren plz apni videos k 😊

[@savitact7960]

Way to represent is excellent, covered all the major points in short video, Thankyou so much

[@sajidmicrobiology]

most welcome

[@ronaldjamatia7119]

may GOD bless u...pls make more videos

[@ivyanyango8170]

This was very helpful and easy to understand. Thank you.

[@Arif05150]

Finally I understand actually what is this. Even in my university I didn't understand this

[@emanuelgrant4335]

That was excellent! Thank you.

[@prabhudasnair5636]

Sirjee u are genius the way u teach excellent after watching ur videos all doubts are crystal clear God bless u brother

[@symoi1569]

you're a Lifesaver!

[@odedile8]

thank you Sajid- great explanation :)

[@bhavanishetty2418]

Very nice explanation sir...but what is the washing agent we have to use in this process?

[@sorlos]

THANK YOU!! Well explained!!

[@jakedolezal871]

@Sajid Shaikh is there a way to determine unlabeled antigen or ligand concentration if there were only two solutions? In other words, the first scenario is only adding a known concentration of labeled antigen to some concentration of antibodies. The radioactivity is measured of bound antibody-antigen molecules after removing free antigen. Then, the experiment is repeated with an unknown amount of unlabeled ligand that is added after adding the same known amount of labeled antigen. Ki = kd = 7.4*10^-6 M and concentration of known labeled antigen = 7.4*10^-6 M. What is concentration of unlabeled antigen added in the repeat experiment? Also, radioactivity of bound antibody in the first experiment = 63000 cpm and radioactivity of bound antibody after removing free labeled and unlabeled antigen = 49000 cpm (counts per minute). I am not sure what Ki in this instance means, i.e. Ki = [Ag][Ab]/[AgAb] ? and kd = [Ag*][Ab]/[Ag*Ab]? (where Ag* = radiolabeled antigen) in which case [Ab] would cancel leaving [Ag]/[AbAg] = [Ag*]/[Ag*Ab]. However, I am not sure that is correct. At any rate, I am not sure how to use the information given in this problem. Please anyone let me know if you "see the light" on how to use this information to find concentration of unlabeled antigen [Ag]. Thanks.

[@reemariaz9397]

Great explanation!

[@zikrarahman8525]

Got it sir... plz explain about microparticle enzyme immune assay

[@pritpalsinghahluwalia715]

Excellent.. A thorough efforts in making us understand the core concept.. Thanku sir

[@sajidmicrobiology]

Most welcome Pritpal .. would you like to help us by donating small amount to our channel? This will motivate us to make more free videos.

[@PreetiGupta-yg9bt]

wow i just loved it.

[@preciousezeobi5770]

You Sir are amazing.... Very wonderful explanation...

[@prateekpanda4224]

You are the better than everyone else 😍😍😍😍😍 shomu is also not that clear but hats off to u man

[@sajidmicrobiology]

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[@nitaprajapati5179]

Great explanation Thank you so much ✨

[@theanatomy774]

best explanation 😎👍👍and helpful for clearing the concept thoroughly 🤠🙏🙏

[@smash2009]

Thank you this helped alot ... keep up the good work :)

[@salmasayeed199]

Excellent..precise and short explanation

[@thecerebrum.]

You’re amazing ❤️

[@Music_Gala]

Sir it was just amazing Please keep on making such amazing vedios

[@sivakumars7842]

Thank you very much for your explanation 🙂🤝

[@pharmacist8622]

You're amazing I'm not speak your language but I understand your explanation because you are excellent ✔

[@sheetal2023Bio]

Very well explained, thank you.

[@rajukambar1902]

Excellent explanation n clear understanding pictures tq so much sir

[@silviaazara8086]

Thank you so Much!!!

[@rajikumari8807]

Wooooow super and clearly explanation 😎👍👌👏👏

[@chakradharwadhekar6112]

Love you this explains

[@dorian_arno]

Great explanation it helped me a lot.

[@yamaatomayi4315]

sir , how this antibody is coated to the microtiter plate..?? and how does isotops are tag to particular antigen ??... plz ans i couldn't find from ne other book also

[@sajidmicrobiology]

For coating , coating buffers like bicarbonate of pH 9 are used... for making particular molecular radiolabelled... radioactive nutrients are supplied to the producer cells

[@felopeeontube]

Fantastic! Thank you!

[@deepakvs2342]

Nice and amazing explanation... Thank you sir...

[@ViralClips981]

Why the labeled antigen is removed ? Is it have low affinity as compared to unknown antigen??

[@armella.h]

thank you! very well explained

[@nagalakshmichallapalli7737]

Just awesome 💯

[@ciroesposito5395]

Could you clarify a doubt, why in this technique separates the free antigens (labeled and not) from those complexed to the antibody (labeled and not)?

[@aliraza-hc4nw]

awessssommmme explanation and verrrry nic sir God bless u

[@jayblack8691]

I'm assuming you know the concentration of the radio labeled antigens? Just saying if you take a blood serum sample how will you determine comparison antigen level without knowing the tagged antigens

[@swatisangwan4925]

Thank you. It really helps.

[@ogroogrom3933]

That's a great help, thank you.

[@sajidmicrobiology]

Most welcome

[@sanusandhyaraina2939]

Thank yu so much for this awesome video

[@alessandroinnocenti235]

Very clear thank you!!

[@imsaiarasan8415]

Thanks sajith

[@aydamaryoud226]

what makes antibody leave labelled Ag and attach to unlabelled?

[@user-wi3nu3cp7z]

Amazing Thank you so much

[@menasalman8534]

Perfect explanation! Thank you!

[@sajidmicrobiology]

Most welcome mena.. would you like to help us by donating small amount to our channel? This will motivate us to make more free videos.

[@menasalman8534]

@@sajidmicrobiology Yes!

[@sajidmicrobiology]

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[@sidrasarwar6721]

Amazing explanation

[@achithswarnachandra365]

Thanks!! Good work👍

[@mahimakapoor9982]

Great explanation. One question though, Why is unlabeled antigen able to displace labeled Ag bound to Ab.... As in why is the unlabeled antigen winning the competition?

[@sajidmicrobiology]

Yes... it's due to competition... like substrate of enzyme and inhibitor

[@mahimakapoor9982]

@@sajidmicrobiology Ohh so it's basically due to high concentration of unlabelled Ag vs labelled one... Thanks

[@AKshay_47]

Thanks sajid Bhai.....It really helped me during exam....

[@sajidmicrobiology]

Most welcome akshay

[@muhammed-kasimonimisisan-ci1co]

Thanks a bunch

[@shubhrasmitatola6951]

Very good explanation thank you❤

[@mitraarjunan49]

How to check the radioactivity?? How you measuring it as 40%?

[@poeticvilla8139]

Very easy plus clearly explained.. thankyou so much sir

[@smithank9347]

Realy thankful video......thank you sir

[@namitanepal798]

Very nicely explained.. thank u so much🙂

[@lovenatureherbalproducts8886]

Excellent video... 👏👏👏👏

[@kaesavr.p1473]

Simply explained.

[@ritujaiswal286]

Your videos now helping in my csir net jrf preparation Thank you ☺☺

[@sajidmicrobiology]

Most welcome

[@pritambeg1124]

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[@gehadfarag9062]

Thank you ❤

[@sayanighosh7872]

Very clear concept. 😊

[@rameshpadha1974]

Thank you so much

[@user-mk8bl1uc8x]

Awesome explanation!

[@sajidmicrobiology]

Thanx... where r u from?

[@user-qt1pg6pb5x]

Excellent explanation sir 🤩

[@laraibkanwal6997]

Thanks bro you made me understand it so easy...jazakallah

[@shinyd6398]

Thanks it was really helpful

[@strivingforexcellency]

Thank you so much for the information sir

[@cricketwarrior1778]

Nice explanation and make a video on fermentation cultures

[@curryfromscratch5286]

You r a life saver

[@sajidmicrobiology]

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[@agarwalshivam844]

Thanks a lot. Really well explained

[@sajidmicrobiology]

Most welcome

[@Megha-ij8eo]

Wonderful explanation😊😘👍

[@chaitrachethanmeena3111]

Sir u r simple and awesome teacher.....thank u for helping us.....🙏🙏🙏

[@sajidmicrobiology]

Most welcome dayana

[@venkatkajal1536]

Thanks for awesome explanation..

[@fredmd101]

Nice. Thank you!

[@melodychao8529]

this is great! thx!

[@sara-almutairi]

sajid, you dope bro. Thankyouu!

[@sajidmicrobiology]

Most welcome 아델사라

[@user-rb4mc3kt5i]

Very clear explanation thanks

[@iqranazir3366]

thank you so much sir nicely explained

[@sakshisood7108]

Hello. The affinity of both the atigens must be similar and conc. Is what we have to find while one is radio labelled and other is not. Why antibody is goving preference to the unlabelled ones when it is already occupied wd the similar type of antigen?

[@elajustina8144]

Because the unlabelled antigens have more affinity to the antibodies

[@sakshisood7108]

Why? Both are similar

[@elajustina8144]

@@sakshisood7108 the monoclonal antibodies are developed against the unlabelled antigen so they have more affinities towards each other than the radio-labelled antigen