Radioimmunoassay (RIA): Animated explanation

[@magnun123456]

Sajid, is seriously, your explanation and visual methods was SO AMAZING, thanks a lot!!!!

[@dr.janpreetsingh5103]

I have seen many videos on this topic but no one explained like this! this is such a nice explanation!!😊

[@DrWilvensJacquesMD]

I missed a question about it in Uworld for USMLE step 1, and their explanation could not help me understand the concept, and you did. Thank you so very much!

[@annag27]

Omg YOU EXPLAINED THIS SO WELL. I get it now.

[@sagorika5552]

I couldn't understand clearly in my class and was very tensed about it cause my exam is on thrusday.But your explanation gave me relief.very effective video......

[@hugodesrosiers-plaisance3156]

Very clear explanation! Thank you!

[@sajidmicrobiology]

Most welcome

[@salmasayeed199]

Excellent..precise and short explanation

[@Immss]

I'm very happy now, all my doubts on RIA are cleared, thank u so much sir..😊

[@Mzee2t]

Thanks a lot. This has helped a lot with my physiology course

[@Arif05150]

Finally I understand actually what is this. Even in my university I didn't understand this

[@rajukambar1902]

Excellent explanation n clear understanding pictures tq so much sir

[@prabhudasnair5636]

Sirjee u are genius the way u teach excellent after watching ur videos all doubts are crystal clear God bless u brother

[@ViralClips981]

Why the labeled antigen is removed ? Is it have low affinity as compared to unknown antigen??

[@theanatomy774]

best explanation 😎👍👍and helpful for clearing the concept thoroughly 🤠🙏🙏

[@AKshay_47]

Thanks sajid Bhai.....It really helped me during exam....

[@sajidmicrobiology]

Most welcome akshay

[@preciousezeobi5770]

You Sir are amazing.... Very wonderful explanation...

[@chandlerbing5437]

Thank you so much ,saved at least an 2to 3 hours of time and energy....

[@savitact7960]

Way to represent is excellent, covered all the major points in short video, Thankyou so much

[@sajidmicrobiology]

most welcome

[@rajikumari8807]

Wooooow super and clearly explanation 😎👍👌👏👏

[@zikrarahman8525]

Got it sir... plz explain about microparticle enzyme immune assay

[@kliusd]

*slowly claps* Well presented. Thank you, Sir. You are a legend.

[@ArvindSingh-jt6sg]

Wow !.. finally got it , amazing skills to aproach the topic 👏👏

[@ritujaiswal286]

Your videos now helping in my csir net jrf preparation Thank you ☺☺

[@sajidmicrobiology]

Most welcome

[@maryem2880]

The part From 7:40 is where the problem has been solved and it's all clear now eventhough the accent is a little hard to understand .. thank u sir!!

[@sajidmicrobiology]

Most welcome.. it hard to talk english for me

[@mahimakapoor9982]

Great explanation. One question though, Why is unlabeled antigen able to displace labeled Ag bound to Ab.... As in why is the unlabeled antigen winning the competition?

[@sajidmicrobiology]

Yes... it's due to competition... like substrate of enzyme and inhibitor

[@mahimakapoor9982]

@@sajidmicrobiology Ohh so it's basically due to high concentration of unlabelled Ag vs labelled one... Thanks

[@IllusionsJunkie]

Thank you, this helped a lot! Excellent work! I'm studying in german, but I could still understand everything very well (:

[@sajidmicrobiology]

Most wecome brother

[@uroojkhan6555]

Best video on KZbin that cleared all concepts waoooo... Urdu version b bnaya kren plz apni videos k 😊

[@zayler_]

Thank you so much ! Your explanation on this topic is phenomenal.

[@pharmacist8622]

You're amazing I'm not speak your language but I understand your explanation because you are excellent ✔

[@ronaldjamatia7119]

may GOD bless u...pls make more videos

[@AdityaRaj-zk9eh]

this the best explanation so far..thanks alot

[@namitanepal798]

Very nicely explained.. thank u so much🙂

[@poeticvilla8139]

Very easy plus clearly explained.. thankyou so much sir

[@pritpalsinghahluwalia715]

Excellent.. A thorough efforts in making us understand the core concept.. Thanku sir

[@sajidmicrobiology]

Most welcome Pritpal .. would you like to help us by donating small amount to our channel? This will motivate us to make more free videos.

[@pritambeg1124]

ASSALAMUALAIKUM দাদা , খুব ভালো বোঝালেন , খুব ভালো লাগল

[@soniamirza4833]

Very informative and your way of teaching is excellent 👍

[@sajidmicrobiology]

Thanx.. whr r u from ?

[@soniamirza4833]

@@sajidmicrobiology lahore

[@sajidmicrobiology]

Ok

[@soniamirza4833]

@@sajidmicrobiology sir how can we measure the radioactivity?

[@sajidmicrobiology]

With the help of scintillation counter

[@Shashwat_Lakhera]

Better than shomu's biology video.

[@mitalogy7108]

U r right 👍

[@arvindhkumar819]

Crystal clear explanation. Tysm!!

[@irissha9675]

I finally understood this technique, you made it very clear. thank you so much !!!

[@avanraws77]

Good job...perfect explanation...keep up the good work!! 👍

[@jayblack8691]

I'm assuming you know the concentration of the radio labeled antigens? Just saying if you take a blood serum sample how will you determine comparison antigen level without knowing the tagged antigens

[@aydamaryoud226]

what makes antibody leave labelled Ag and attach to unlabelled?

[@bhavanishetty2418]

Very nice explanation sir...but what is the washing agent we have to use in this process?

[@sivakumars7842]

Thank you very much for your explanation 🙂🤝

[@mitraarjunan49]

How to check the radioactivity?? How you measuring it as 40%?

[@sayanighosh7872]

Very clear concept. 😊

[@sakshisood7108]

Hello. The affinity of both the atigens must be similar and conc. Is what we have to find while one is radio labelled and other is not. Why antibody is goving preference to the unlabelled ones when it is already occupied wd the similar type of antigen?

[@elajustina8144]

Because the unlabelled antigens have more affinity to the antibodies

[@sakshisood7108]

Why? Both are similar

[@elajustina8144]

@@sakshisood7108 the monoclonal antibodies are developed against the unlabelled antigen so they have more affinities towards each other than the radio-labelled antigen

[@Music_Gala]

Sir it was just amazing Please keep on making such amazing vedios

[@aliraza-hc4nw]

awessssommmme explanation and verrrry nic sir God bless u

[@jakedolezal871]

@Sajid Shaikh is there a way to determine unlabeled antigen or ligand concentration if there were only two solutions? In other words, the first scenario is only adding a known concentration of labeled antigen to some concentration of antibodies. The radioactivity is measured of bound antibody-antigen molecules after removing free antigen. Then, the experiment is repeated with an unknown amount of unlabeled ligand that is added after adding the same known amount of labeled antigen. Ki = kd = 7.4*10^-6 M and concentration of known labeled antigen = 7.4*10^-6 M. What is concentration of unlabeled antigen added in the repeat experiment? Also, radioactivity of bound antibody in the first experiment = 63000 cpm and radioactivity of bound antibody after removing free labeled and unlabeled antigen = 49000 cpm (counts per minute). I am not sure what Ki in this instance means, i.e. Ki = [Ag][Ab]/[AgAb] ? and kd = [Ag*][Ab]/[Ag*Ab]? (where Ag* = radiolabeled antigen) in which case [Ab] would cancel leaving [Ag]/[AbAg] = [Ag*]/[Ag*Ab]. However, I am not sure that is correct. At any rate, I am not sure how to use the information given in this problem. Please anyone let me know if you "see the light" on how to use this information to find concentration of unlabeled antigen [Ag]. Thanks.

[@deepakvs2342]

Nice and amazing explanation... Thank you sir...

[@ciroesposito5395]

Could you clarify a doubt, why in this technique separates the free antigens (labeled and not) from those complexed to the antibody (labeled and not)?

[@trilochanabalivada]

Excellent explanation sir 🤩

[@sathyachouthri6556]

Very good teaching🙂👌

[@jagritiverma4308]

I doesn't get the plotting of graph

[@junaidchoudhury7292]

Is the amount of antibody kept constant as there is increase in unlabelled antigen concentration

[@sajidmicrobiology]

Yes junaid....

[@junaidchoudhury7292]

suppose there are 5 antibodies on the well ..so there will be 10 binding sites for antigens to bind to the antibody.. that means only 10 antigens will bind to the antibody.. no matter to what extent we increase the concentration..now how can increase in concentration make difference..As only 10 antigens are going to bind with that 5 antibody..can u plz explain

[@sajidmicrobiology]

Dear junaid... for digramatic illustration I have show only few antibodies.. practically there are thousands of Abs... but yes.... after a certain conc of Ag, Abs are saturated.... and it should be ...

[@yamaatomayi4315]

sir , how this antibody is coated to the microtiter plate..?? and how does isotops are tag to particular antigen ??... plz ans i couldn't find from ne other book also

[@sajidmicrobiology]

For coating , coating buffers like bicarbonate of pH 9 are used... for making particular molecular radiolabelled... radioactive nutrients are supplied to the producer cells

[@chaitrachethanmeena3111]

Sir u r simple and awesome teacher.....thank u for helping us.....🙏🙏🙏

[@sajidmicrobiology]

Most welcome dayana

[@kavitachawla3306]

radioimmunoassay can be employed for the detection of insulin in blood plasma for this 125I labelled insulin is mixed and allowed to bind with a known concentration of anti insulin antibody. A known volume of patient blood plasma is then added to the conjugate and allowed to compete with the antigen binding site of antibody. the bound antigen is then separated from the unbound ones and radioactivity of free antigen is then measured by Gama counter . following are some the statement made about this essay . 1 the ratio of radioactive count for the unbound antigen to the bound one is more at the end of reaction. 2.the ratio of radioactive count for unbound antigen to the bound one is less at the end of the reaction. 3 the diabetic patient, the radioactive count for free antigen is less than that for a normal individual 4 A diabetic patient the radioactive count for the free antigen is more than that for the normal individual which of which of the above statement are true?....... Plz sir ans this question in some short explanation plzzzzzzzz

[@sajidmicrobiology]

Option 1 and 4 seem to be correct ...

[@kavitachawla3306]

Thank u sir

[@preeti-bq7st]

Very nicely explained 😍

[@lovenatureherbalproducts8886]

Excellent video... 👏👏👏👏

[@shubhrasmitatola6951]

Very good explanation thank you❤

[@prateekpanda4224]

You are the better than everyone else 😍😍😍😍😍 shomu is also not that clear but hats off to u man

[@sajidmicrobiology]

Learn immunology (live, online personal/group couching ) contact : [e-mail]

[@shivanisah1850]

Thanx sir ... u explained it in very easy way

[@sajidmicrobiology]

Most welcome Shivani Duggal

[@soco2020]

Amazing explanation. Thank you!

[@doctorofmedicine1113]

Sajid both ur name and video are great

[@sajidmicrobiology]

Imran Malla Thanx ☺

[@doctorofmedicine1113]

Are u from Kashmir i think so

[@sajidmicrobiology]

Maharashtra

[@Ismail-dy1wt]

May Allah bless you brother. very comprhensive

[@sajidmicrobiology]

Thanks for appreciation.. Would you like to encourage our team by donating small amount (Rs. 5 to 100)?

[@Ismail-dy1wt]

@@sajidmicrobiology how can I donate?

[@sajidmicrobiology]

Thanx for giving positive response..you can either paytm us on 8830061003 or google pay on same number (UPI ID - saj.99999@oksbi) Your small help can make big diffrence.. Allah hafiz

[@ImranAhmad-df3mi]

Nyc explanation sir

[@Megha-ij8eo]

Wonderful explanation😊😘👍

[@sakthidharshni5975]

thank you sajid . it is really amazing video .pls do more videos

[@sajidmicrobiology]

Most welcome Sure...

[@sakthidharshni5975]

thank you

[@laraibkanwal6997]

Thanks bro you made me understand it so easy...jazakallah

[@ivyanyango8170]

This was very helpful and easy to understand. Thank you.

[@hanssambodo9683]

discryption?

[@MikeBassil]

Simpel clear and video wasn't too long, thank you!

[@sidrasarwar6721]

Amazing explanation

[@somosreeghosh8662]

Thanku..u made it very simple...1 question how to detect radio activity?

[@sajidmicrobiology]

Thanx Somosree

[@sajidmicrobiology]

Radioactivity is tested by scintillator counter

[@somosreeghosh8662]

Sajid Shaikh thanku again :)

[@symoi1569]

you're a Lifesaver!

[@cricketwarrior1778]

Nice explanation and make a video on fermentation cultures

[@shaimaasobhy7218]

Thanks alot itis amazing . but I need to ask how can i measure the radioactivity??

[@sajidmicrobiology]

scintillation counter

[@96.y.vaishnavi60]

Nice explanation 👌👌👌

[@razanathamneh7906]

Incredible explanation, thank u 🙏🏻 😊

[@sajidmicrobiology]

Most welcome razan .. would you like to help us by donating small amount to our channel? This will motivate us to make more free videos.

[@odedile8]

thank you Sajid- great explanation :)

[@nitaprajapati5179]

Great explanation Thank you so much ✨

[@Jaysay7]

Awesome explanation!

[@sajidmicrobiology]

Thanx... where r u from?

[@supriyapathak6645]

Very easy explanation nice video

[@janamsrinath2471]

Excellent bro we want some more detailed way

[@imsaiarasan8415]

Thanks sajith

[@your_babai_rj]

Great explanation it helped me a lot.

[@aurora4901]

Very good video.. thank you😊

[@menasalman8534]

Perfect explanation! Thank you!

[@sajidmicrobiology]

Most welcome mena.. would you like to help us by donating small amount to our channel? This will motivate us to make more free videos.

[@menasalman8534]

@@sajidmicrobiology Yes!

[@sajidmicrobiology]

Thanx mena.. you can paytm us on 8830061003. Your small help can make big diffrence.

[@mohd_athar123]

i have watched many videos on youtube but no one can teach like you. thank u sir for well detailed explanation.

[@saguasodariya5585]

You are Exllent dear pan upload more video

[@Megha-ij8eo]

Nice explanation sir..😊😘👌

[@sajidmicrobiology]

Thanx

[@shaikhtehreembano9683]

Sir can you please tell me hoW to wash...for removing that antigens which are non bound

[@sajidmicrobiology]

With the help of buffer

[@shaikhtehreembano9683]

@@sajidmicrobiology thank you sir

[@shaikhtehreembano9683]

@@sajidmicrobiology sir if we are using this technique for determining the concentration of T3 and T4 hormones...then how can we say that the serum sample is antigen...bcoz we are adding it as a unlabelled antigens??

[@sajidmicrobiology]

daddy's devil ... I didn't got the question

[@shaikhtehreembano9683]

@@sajidmicrobiology sir m saying that in RIA we add labelled antigens and at the place of unlabelled antigens we will add serum sample....what if we have to identify the conc of hormones in the serum...we can't call serum as unlabelled antigens bcoz they are produced by our body...I was asked this question in seminar but I was not able to answer

[@II-tc7of]

Nice explanation. Sir please make a video on RFLP (POLYMORPHISM OF RESTRICTION FRAGMENT)

[@sajidmicrobiology]

Sure Raghuveer

[@iqrasid8100]

Very informative

[@agarwalshivam844]

Thanks a lot. Really well explained

[@sajidmicrobiology]

Most welcome

[@mayragarzat263]

yo no hablo ingles ni entiendo muy bien el ingles, pero me super encanto, con las imágenes es muy entendible todo, gracias.

[@PreetiGupta-yg9bt]

wow i just loved it.

[@tanishadhyani6846]

Very good explanation

[@sajidmicrobiology]

Thanx tanisha