Indirect ELISA: Animated explanation

[@animal_expert]

Thank you Sir! Very helpful that you included the case where the primary antibodies aren't specific to the antigens to see the comparison.

[@kyramirchandani7270]

You explained the toughest part in simplest way.hats off sir

[@sajidmicrobiology]

Thanx kyra. If you like our videos, Please donate us to keep work going on. You small help (starting form Rs. 5) is valuable for us. Paytm/Google pay number 8830061003.

[@homosapien682]

The Best Ever Explanation One can Get. Precise

[@nicknicky2001]

THANK YOU SO MUCH! Your video was fast, simple, and informational. I appreciate you.

[@moniraafrozmoli2626]

best ever explained...thank you for explaining it so well.

[@debdeepchakraborty8859]

Your lectures contains more amount of data , your vocals are easy for us to understand ..... 💓

[@abelurbina2003]

It is clear now that when the chromagen interacts with the antibody with an enzyme the chromagen dissolve and produces colorization in the entire glass. What I am thinking before during the interactions of chromagen to an antibody with an enzyme, the small portion only of the antibody will only produce colored light.

[@nathanrussell1538]

A really precise and pedagogical explanation. Well done.

[@joan-b-empire576]

Thank you from Cameroon 🇨🇲❤️

[@hemantk345]

well the way you teaching is awesome great work buddy keep going..... i would like to add a question that after binding of antibodies to antigen ( specific non covalent reaction occurs between Ag-Ab i.e epitope and paratope respectively) the secondary enzyme linked antibodies binds to primary antibody of fc region but as far i know two antibodies cannot react and binds each other then what basic principle holds this situation?? looking forward to your reply thank you

[@sajidmicrobiology]

As u said.. that antigens have epitopes... so when our antibody is injected in the lab animal, that antibody acts as antigen for that animal... so this antigen (our antibody) will also have epitopes on its surface including fc region also which are called isotypic epitopes... so lab animal can produce antibody aginst these epitopes

[@hemantk345]

@@sajidmicrobiology thank you soo much buddy

[@sciencenerd752]

this topic wasn't even clear in wilson and walker but you explained it very well. thank you

[@sajidmicrobiology]

Thanx.. would you like to help our channel by donating small amount (Rs 5 to 100)? This will encourage out team to make more videos

[@m.gailstotler525]

Excellent explanation/instruction. [Antibodies is misspelled at 6:15 on the video.]

[@MdAli-rc3mc]

Assalamoalaikum. Brother! I enjoy your lecture very much. Please keep it up. May Allah livelong you. Thank you

[@sajidmicrobiology]

Walekumasslam... most welcome

[@Dr.khalid-r8n]

Best explanation I swear the doctor who give me the lecture don’t know what to do I red the lecture for for 6 Hours and didn’t understand what my doctor was saying While you explain it in few minutes You are the best♥️♥️♥️

[@sajidmicrobiology]

Most welcome

[@Mohitthakurofficial]

Excellent. Your video explanations are very simple and easy to understand. Your method of teaching is quite impressive. Please upload more videos on other techniques as well. I subscribed. Your channel need more subscribers.

[@sajidmicrobiology]

Thanx Mohit ... i am in the process of uploading

[@abidabibi3317]

best explaination and helped me before my presentation 😊

[@namitma]

The sample we are putting in the titer after the coating buffer. Is the sample just the blood of the person?

[@sajidmicrobiology]

Its not whole blood... it is serum most of the time

[@shatikkulaminh643]

Best immunology video I hv ever seen. .great job ..😊😊😊

[@Mohitthakurofficial]

Sir abhi tak naye videos nahin aaye. Please upload as soon as possible, I'm eagerly waiting. Please upload haematology, serology, pathology, biochemistry, mol bio techniques.

[@rathodlal1385]

Initially from where we have to take antigen...? Which are attaching to microplate surface.

[@sajidmicrobiology]

Yes

[@rathodlal1385]

Ur not understood my question. Ooo antigen kidarse aya

[@rathodlal1385]

Artificially availability is there of antigen

[@sajidmicrobiology]

Agar apko koi antibody detect karni hain to apke pass uss antibody ka specific antigen purified form me chahiye..

[@sajidmicrobiology]

Agar koi investigation me anti hiv antibodies detect karne ho. to investigators ke pass hiv antigens hote hain jo invitro synthesize kiye jate hain ya fir kisi source se purify kiye jate hain... kits main ye antigens company provide karti hain

[@ciroesposito5395]

Is it possible to do the opposite by putting the antibody bound to the polystyrene plate to determine the antigen concentration in the sample?

[@sajidmicrobiology]

Yes

[@ciroesposito5395]

@@sajidmicrobiology ok thanks

[@sharonjitkaur8411]

what would you say the ease and speed of the process is? how long does it take to perform indirect elisa?

[@keepmovingupendra4691]

Excellent explanation with diagrams

[@aliraza-hc4nw]

veryy hardworking masahaalh keep it up sir ap electrophoresis pa bhi video upload krya

[@mdkawser5373]

Best explanation. Thank you

[@itsmejosh7045]

Bro your teaching method is too good and perfect

[@sajidmicrobiology]

Most welcome Joshwa .. would you like to help us by donating small amount to our channel? This will motivate us to make more free videos.

[@abelurbina2003]

Sir the chromagen color dissolve inside the glass or produces colored light? Thanks

[@sajidmicrobiology]

It dissolves

[@kaustubhrege969]

Thanks for the guidance

[@DejavuDejavu]

It's well explained and it's really helpful. Thanks for the video and hope you can do more. LOVEYOURSELF ♥️

[@simransarkar4502]

Why are we adding the acid when we already getting the color by substrate addition only

[@sajidmicrobiology]

To stop the reaction

[@rancysidharthan8287]

Well presented... excellent..

[@nyawirawaithaka4993]

Excellent explanation! Thank you

[@meghanac6128]

Awesome

[@saguasodariya5585]

You are brilliant ...Brooklyn continue. with new video

[@sajidmicrobiology]

Thanx

[@ssss...7896]

tq so much sr..concept clear n easy explanation

[@sakshichand1126]

Sir will you plz conduct lecture on how to take notes from textbooks

[@m.thishan191]

Very nice 👍🏼

[@sugarboo9544]

Nice presentation, very easy to understand. Thanks a lot.

[@shreyakatiyar9041]

Amazing👍🏻

[@alinarajpoot731]

THANK YOU SO MUCH THIS VIDEO WAS REALLY REALLY HELPRFUL ...... EXCELLENT

[@sajidmicrobiology]

Most welcome

[@sajidmicrobiology]

If you like our videos, Please donate us to keep work going on. You small help (starting form Rs. 5) is valuable for us. Paytm number 8830061003

[@jadumonigogoi845]

has secondary antibody gain antigen property after binding enzyme to bind primary antibody!!

[@sajidmicrobiology]

Every molecule including antibody or enzyme can act as antigen when injected to lab animal. If we inject our antibodies to mice.. it will act as antigen

[@thaniabremer7255]

Very clear discussion!! Thank U Sir:'>

[@sukanyachakraborty2678]

Brilliant explanation 💚

[@hasenmothi8752]

Sir update more videos. It is very useful to understand with animation.

[@sajidmicrobiology]

Sure

[@anna-kb9mn]

You’re amazing! Thank you so much :)

[@vatsala1902]

Thanku so much. Explanation is too good 😊

[@sajidmicrobiology]

Vatsala sharma most welcome

[@Shobhi_pearl]

Explained very well 😊

[@poonamjhamb5807]

Very very helpful video 👏👏👏

[@luiff9884]

Very nice explanation sir thanks

[@anjuvenkidsaraswathy6785]

Well explained ..thank you.

[@Zhi-u6j]

Great explanation thanks

[@binbinky78]

Excellent explanation, thank you 👍😀

[@sajidmicrobiology]

Thanx

[@aminuyunusa40]

Very nice indirect Elisa lecture

[@jatinjacks1449]

Well explained! Hats of to you sir.

[@rubabk1603]

Very well-explained! 😊 👍

[@robertjamestaylor9261]

best explanation

[@shomeshali5675]

Nice

[@jyotisingh878]

very nice video sir thanks 👌👌👌☺️☺️

[@dr.devendrabhardwaj1836]

Very good explanation

[@rimalshahid3141]

Great

[@p.j.shruthi7198]

Very well explained.

[@aniruddh-chakravorty]

Where is video of direct elsiaa?

[@kvnkvn9191]

What an explanation!

[@jojo-my8bu]

Really well explained

[@sajidmicrobiology]

Thanx

[@سلميعسكر-د1ك]

That was perfect ,tnx so much

[@lakshmankaranam]

Is it 4 generation or third

[@vijayachandrakalyani779]

Very well explained. Tq sir

[@sajidmicrobiology]

Most welcome vijayachandra .. would you like to help us by donating small amount to our channel? This will motivate us to make more free videos.

[@achintyasasmal4471]

💞👍💞

[@YourAhmad]

thank u so much.... you did a great job...😊

[@sajidmicrobiology]

Most welcome

[@alakeshbharali5246]

Too gooddd

[@abh3835]

Good explanation thanks

[@meeromeer8812]

Thanks a lot.

[@ImranAhmad-df3mi]

Excellent thanks so much 😘 sir

[@sajidmicrobiology]

Most welcome

[@mayankmehendiratta999]

difference between sandwich anf this one

[@surachanarai4064]

very nicely explained.. thank u sir.

[@sajidmicrobiology]

Most welcome

[@bappabera2465]

Just woaw

[@sohadalawer5808]

Thank u so much

[@rst6723]

Good explanation

[@omarhalimi1685]

This was so great

[@samikshyaacharya1032]

Thank you so much😊

[@leenusn]

Best ever

[@medzoklife]

I subscribed.

[@bossyhesham6423]

thanks alot ❤

[@sukritidhaliwal8802]

Well explained!.. Thanks.

[@Smritysays]

Thank you for this

[@karripradeepkumar911]

Tqq so much

[@yasmineyasmine6010]

thank you so much this was very helpful 🤍🤍

[@sajidmicrobiology]

Most welcome

[@ImranAhmad-df3mi]

Nyc sir

[@forumedicos5202]

Tysm sir

[@kritikajaiswal4945]

Thank you ☺☺

[@tarteelmohammed3257]

Thank you

[@afrozaga4464]

Direct also plzz sir

[@sajidmicrobiology]

Sure

[@afrozaga4464]

@@sajidmicrobiology thankyou so much sir.

[@rishishet3330]

Awesome

[@silentclaw8011]

Awesome