Indirect ELISA: Animated explanation

[@animal_expert]

Thank you Sir! Very helpful that you included the case where the primary antibodies aren't specific to the antigens to see the comparison.

[@kyramirchandani7270]

You explained the toughest part in simplest way.hats off sir

[@sajidmicrobiology]

Thanx kyra. If you like our videos, Please donate us to keep work going on. You small help (starting form Rs. 5) is valuable for us. Paytm/Google pay number 8830061003.

[@homosapien682]

The Best Ever Explanation One can Get. Precise

[@moniraafrozmoli2626]

best ever explained...thank you for explaining it so well.

[@joan-b-empire576]

Thank you from Cameroon 🇨🇲❤️

[@nicknicky2001]

THANK YOU SO MUCH! Your video was fast, simple, and informational. I appreciate you.

[@abelurbina2003]

It is clear now that when the chromagen interacts with the antibody with an enzyme the chromagen dissolve and produces colorization in the entire glass. What I am thinking before during the interactions of chromagen to an antibody with an enzyme, the small portion only of the antibody will only produce colored light.

[@nathanrussell1538]

A really precise and pedagogical explanation. Well done.

[@debdeepchakraborty8859]

Your lectures contains more amount of data , your vocals are easy for us to understand ..... 💓

[@abidabibi3317]

best explaination and helped me before my presentation 😊

[@shatikkulaminh643]

Best immunology video I hv ever seen. .great job ..😊😊😊

[@keepmovingupendra4691]

Excellent explanation with diagrams

[@ciroesposito5395]

Is it possible to do the opposite by putting the antibody bound to the polystyrene plate to determine the antigen concentration in the sample?

[@sajidmicrobiology]

Yes

[@ciroesposito5395]

@@sajidmicrobiology ok thanks

[@m.gailstotler525]

Excellent explanation/instruction. [Antibodies is misspelled at 6:15 on the video.]

[@rancysidharthan8287]

Well presented... excellent..

[@simransarkar4502]

Why are we adding the acid when we already getting the color by substrate addition only

[@sajidmicrobiology]

To stop the reaction

[@mdkawser5373]

Best explanation. Thank you

[@namitma]

The sample we are putting in the titer after the coating buffer. Is the sample just the blood of the person?

[@sajidmicrobiology]

Its not whole blood... it is serum most of the time

[@hemantk345]

well the way you teaching is awesome great work buddy keep going..... i would like to add a question that after binding of antibodies to antigen ( specific non covalent reaction occurs between Ag-Ab i.e epitope and paratope respectively) the secondary enzyme linked antibodies binds to primary antibody of fc region but as far i know two antibodies cannot react and binds each other then what basic principle holds this situation?? looking forward to your reply thank you

[@sajidmicrobiology]

As u said.. that antigens have epitopes... so when our antibody is injected in the lab animal, that antibody acts as antigen for that animal... so this antigen (our antibody) will also have epitopes on its surface including fc region also which are called isotypic epitopes... so lab animal can produce antibody aginst these epitopes

[@hemantk345]

@@sajidmicrobiology thank you soo much buddy

[@poonamjhamb5807]

Very very helpful video 👏👏👏

[@itsmejosh7045]

Bro your teaching method is too good and perfect

[@sajidmicrobiology]

Most welcome Joshwa .. would you like to help us by donating small amount to our channel? This will motivate us to make more free videos.

[@nyawirawaithaka4993]

Excellent explanation! Thank you

[@sciencenerd752]

this topic wasn't even clear in wilson and walker but you explained it very well. thank you

[@sajidmicrobiology]

Thanx.. would you like to help our channel by donating small amount (Rs 5 to 100)? This will encourage out team to make more videos

[@ssss...7896]

tq so much sr..concept clear n easy explanation

[@rathodlal1385]

Initially from where we have to take antigen...? Which are attaching to microplate surface.

[@sajidmicrobiology]

Yes

[@rathodlal1385]

Ur not understood my question. Ooo antigen kidarse aya

[@rathodlal1385]

Artificially availability is there of antigen

[@sajidmicrobiology]

Agar apko koi antibody detect karni hain to apke pass uss antibody ka specific antigen purified form me chahiye..

[@sajidmicrobiology]

Agar koi investigation me anti hiv antibodies detect karne ho. to investigators ke pass hiv antigens hote hain jo invitro synthesize kiye jate hain ya fir kisi source se purify kiye jate hain... kits main ye antigens company provide karti hain

[@saguasodariya5585]

You are brilliant ...Brooklyn continue. with new video

[@sajidmicrobiology]

Thanx

[@Mohitthakurofficial]

Sir abhi tak naye videos nahin aaye. Please upload as soon as possible, I'm eagerly waiting. Please upload haematology, serology, pathology, biochemistry, mol bio techniques.

[@m.thishan191]

Very nice 👍🏼

[@sukanyachakraborty2678]

Brilliant explanation 💚

[@sharonjitkaur8411]

what would you say the ease and speed of the process is? how long does it take to perform indirect elisa?

[@abelurbina2003]

Sir the chromagen color dissolve inside the glass or produces colored light? Thanks

[@sajidmicrobiology]

It dissolves

[@sugarboo9544]

Nice presentation, very easy to understand. Thanks a lot.

[@shreyakatiyar9041]

Amazing👍🏻

[@aliraza-hc4nw]

veryy hardworking masahaalh keep it up sir ap electrophoresis pa bhi video upload krya

[@Dr.khalid-r8n]

Best explanation I swear the doctor who give me the lecture don’t know what to do I red the lecture for for 6 Hours and didn’t understand what my doctor was saying While you explain it in few minutes You are the best♥️♥️♥️

[@sajidmicrobiology]

Most welcome

[@Mohitthakurofficial]

Excellent. Your video explanations are very simple and easy to understand. Your method of teaching is quite impressive. Please upload more videos on other techniques as well. I subscribed. Your channel need more subscribers.

[@sajidmicrobiology]

Thanx Mohit ... i am in the process of uploading

[@jadumonigogoi845]

has secondary antibody gain antigen property after binding enzyme to bind primary antibody!!

[@sajidmicrobiology]

Every molecule including antibody or enzyme can act as antigen when injected to lab animal. If we inject our antibodies to mice.. it will act as antigen

[@Shobhi_pearl]

Explained very well 😊

[@alinarajpoot731]

THANK YOU SO MUCH THIS VIDEO WAS REALLY REALLY HELPRFUL ...... EXCELLENT

[@sajidmicrobiology]

Most welcome

[@sajidmicrobiology]

If you like our videos, Please donate us to keep work going on. You small help (starting form Rs. 5) is valuable for us. Paytm number 8830061003

[@binbinky78]

Excellent explanation, thank you 👍😀

[@sajidmicrobiology]

Thanx

[@MdAli-rc3mc]

Assalamoalaikum. Brother! I enjoy your lecture very much. Please keep it up. May Allah livelong you. Thank you

[@sajidmicrobiology]

Walekumasslam... most welcome

[@rubabk1603]

Very well-explained! 😊 👍

[@thaniabremer7255]

Very clear discussion!! Thank U Sir:'>

[@vatsala1902]

Thanku so much. Explanation is too good 😊

[@sajidmicrobiology]

Vatsala sharma most welcome

[@luiff9884]

Very nice explanation sir thanks

[@kaustubhrege969]

Thanks for the guidance

[@sakshichand1126]

Sir will you plz conduct lecture on how to take notes from textbooks

[@jatinjacks1449]

Well explained! Hats of to you sir.

[@anjuvenkidsaraswathy6785]

Well explained ..thank you.

[@aniruddh-chakravorty]

Where is video of direct elsiaa?

[@سلميعسكر-د1ك]

That was perfect ,tnx so much

[@jyotisingh878]

very nice video sir thanks 👌👌👌☺️☺️

[@hasenmothi8752]

Sir update more videos. It is very useful to understand with animation.

[@sajidmicrobiology]

Sure

[@lakshmankaranam]

Is it 4 generation or third

[@aminuyunusa40]

Very nice indirect Elisa lecture

[@YourAhmad]

thank u so much.... you did a great job...😊

[@sajidmicrobiology]

Most welcome

[@DejavuDejavu]

It's well explained and it's really helpful. Thanks for the video and hope you can do more. LOVEYOURSELF ♥️

[@ImranAhmad-df3mi]

Excellent thanks so much 😘 sir

[@sajidmicrobiology]

Most welcome

[@Zhi-u6j]

Great explanation thanks

[@dr.devendrabhardwaj1836]

Very good explanation

[@anna-kb9mn]

You’re amazing! Thank you so much :)

[@vijayachandrakalyani779]

Very well explained. Tq sir

[@sajidmicrobiology]

Most welcome vijayachandra .. would you like to help us by donating small amount to our channel? This will motivate us to make more free videos.

[@p.j.shruthi7198]

Very well explained.

[@robertjamestaylor9261]

best explanation

[@surachanarai4064]

very nicely explained.. thank u sir.

[@sajidmicrobiology]

Most welcome

[@jojo-my8bu]

Really well explained

[@sajidmicrobiology]

Thanx

[@achintyasasmal4471]

💞👍💞

[@abh3835]

Good explanation thanks

[@kvnkvn9191]

What an explanation!

[@silentclaw8011]

Awesome

[@shomeshali5675]

Nice

[@rimalshahid3141]

Great

[@alakeshbharali5246]

Too gooddd

[@sukritidhaliwal8802]

Well explained!.. Thanks.

[@rst6723]

Good explanation

[@samikshyaacharya1032]

Thank you so much😊

[@leenusn]

Best ever

[@omarhalimi1685]

This was so great

[@bossyhesham6423]

thanks alot ❤

[@yasmineyasmine6010]

thank you so much this was very helpful 🤍🤍

[@sajidmicrobiology]

Most welcome

[@bappabera2465]

Just woaw

[@mayankmehendiratta999]

difference between sandwich anf this one

[@medzoklife]

I subscribed.

[@ImranAhmad-df3mi]

Nyc sir

[@kritikajaiswal4945]

Thank you ☺☺

[@sohadalawer5808]

Thank u so much

[@meeromeer8812]

Thanks a lot.

[@Smritysays]

Thank you for this

[@afrozaga4464]

Direct also plzz sir

[@sajidmicrobiology]

Sure

[@afrozaga4464]

@@sajidmicrobiology thankyou so much sir.

[@karripradeepkumar911]

Tqq so much

[@forumedicos5202]

Tysm sir

[@tarteelmohammed3257]

Thank you

[@rishishet3330]

Awesome

[@meghanac6128]

Awesome