Recombinant protein

[@MuhmmedNadeem-cy2tj]

Dude, from all those weird Indians making subjects worse on KZbin, never thought there would be one who could help me. Till I found your channel. +1Like You are awesome mate!! Thanks alot!

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@uncannyskrib937]

My PI is investigating the use of a recombinant hACE2 protein as a potential delivery system of anti-SARS-CoV-2 drugs and I used this video as a refresher to help write a summary paper for this idea. Just last semester I was using your videos to study for my final exams, and now I'm using them to potentially help others develop a treatment for the novel coronavirus. Thank you!

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@albertmontes11]

Thank you. You gave me a basic understanding of monoclonal process creating proteins. Keep instructing!!!

[@shomusbiologyofficial]

Thank you

[@valeriehollstein5356]

all your tutorials I have viewed from you are very well-explained. Thanks a bunch!

[@shirasagi9390]

love your videos. every time i dont understand something i watch your channel and it becomes crystal clear. thank you so much

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@eslamyousef6131]

your a legend bro doing everyone a massive favor, keep up the hard work

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@gangasasikumar2283]

Thank you sir. It was amazing. Im watching this on the day before my examination.. It makes me cool.. Thank you so much sir.. Keep going ❤️

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@vishwaspethe3275]

Very clear explanation of what a recombinant protein is. Thank you for publishing the video.

[@zoypuff]

Thanks, very helpful and simple to understand

[@maroonhorizon1693]

WONDERFULLLLL! ABSOLUTLEY AMAZINGGGG

[@shomusbiologyofficial]

Thank you

[@saraassar5264]

Very informative. I liked your energy!

[@shomusbiologyofficial]

Thank you

[@noorulain7302]

Greatly helpful....... Thanks for your efforts bravo

[@shomusbiologyofficial]

You're welcome

[@TheRockstar-gk9xp]

Thanks Shomu ,God Bless You

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@dakota1432]

I needed to learn a basic understanding of plasmids and this video was beyond helpful. Keep it up man, if you need help with marketing videos let me know.

[@sajidahussain7406]

Thank you sir.. Your way of delivery is so easy and understandable by us easily

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@sajidahussain7406]

@@shomusbiologyofficial you deserve it sir

[@deekshithasubrahmanian5645]

Sir You are really amazing, and its really helpfull your videos to study , thank you somuch

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures and

[@tb2748]

could you make a video on what fusion protein is? and on what protein/affinity tags are? your lectures are very helpful!

[@lobbes44]

This was really really good explained, i now can pass my exam!

[@shomusbiologyofficial]

All the best

[@alondradelgado4630]

What a great video! Thank you, Shomu

[@shomusbiologyofficial]

Thank you

[@RoyBattyLives]

Thank you Shomu, you are a great teacher

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@miguelguerreiro1212]

You saved the day! much better than my teacher

[@arulexport2701]

Wow what a simple great explanation ''' such a huge topic every one easily understood thank u sir

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@jagrityesingh4940]

u ve excellent teaching style.. its very helpful....

[@shomusbiologyofficial]

+jagritye singh thank you. Glad you liked my lectures

[@abirhasanpranto7593]

👌👌

[@keshavsingh2078]

Very helpful . Thank you

[@foggedgoggles]

Thank you so much your videos are so helpful!

[@shomusbiologyofficial]

+Sharon Joseph thank you. Glad you liked my lectures

[@sumanthota7356]

Thank You so much for your wonderful explanation 👌👍👏

[@shomusbiologyofficial]

You're welcome. Glad you liked it

[@zamilkisekka7701]

Great stuff. U made it look very simple

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@johnloubbardi1034]

very helpful explanation video. Thank you

[@shomusbiologyofficial]

You're welcome

[@iffathtaskeen9332]

sir.. The best explaination... Thanks sir....

[@shomusbiologyofficial]

You're welcome

[@mayukhroy5393]

Thank You Sir, this video was very helpful.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@qudratfikrat2023]

Very well explained, thank you!

[@shomusbiologyofficial]

You're welcome

[@mercedesgarciacarrillo1736]

Love this channel 🧡

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@naveenantony7849]

9:00 if the plasmid is capable of self-replicating itself and provide us with multiple copies of the "gene of interest" why should we bother to insert the plasmid into an Ecoli bacteria??

[@sangeetadevi5819]

Plasmids are extra-chromosomal ,self replicating ,double stranded circular DNA molecules ...u know that ?! Ryt. So ,it would replicate when it is in the cell ,...

[@naveenantony7849]

@@sangeetadevi5819 i appreciate your reply👍🏼,but what is the purpose of inserting the plasmid to a bacteria (Ecoli here)..

[@sangeetadevi5819]

This is the whole biotech is all about !! Using microbes , plant/animal cells / their parts(maybe enzymes also) to get the products useful for human beings .🙃🙃

[@sangeetadevi5819]

The bacteria (E.coli ..in this particular case) ,the plasmid DNA ..all are used to produce the products we require.

[@techheaven007]

because it is only capable to replicate inside E.coli..and it requires the cell machinery to replicate ! if we take a shuttle vector that works both in both E.coli and an eukaryotic cell like yeast then we can insert that recombinant plasmid to the yeast also ! its depending on the protein you want to get.. if its an animal or human protein that needs post transcriptional modification, you have to transfer the vector to an eukaryotic cell otherwise a prokaryotic cell is fine..

[@deechan623]

I love this. Thank you so much!!!

[@shomusbiologyofficial]

You're welcome

[@nishi782]

awssmmm teaching style and easily understandable lecture

[@shomusbiologyofficial]

+Swarnika Kumari thank you. Glad you liked my lectures

[@nickynicky8184]

Very useful for mee 🙏🏼🙏🏼🙏🏼

[@shomusbiologyofficial]

You're welcome

[@bornitadeb8282]

Awesome...just amazing

[@shomusbiologyofficial]

Thank you

[@Hipnot]

Thanks a lot ! Nice and clear explanation !

[@shomusbiologyofficial]

You're welcome

[@ignatiousramaano1636]

Well explained🙌🏾

[@shomusbiologyofficial]

Thank you

[@shscompany1188]

you're our big hero

[@shomusbiologyofficial]

+shs company glad I can help

[@heydariparisa1756]

very well explained. Thank you very much

[@shomusbiologyofficial]

+Heydari Parisa thank you. Glad you liked my lectures

[@mayukhroy5393]

Thank You Sir, for this video.

[@shomusbiologyofficial]

You're welcome

[@mariyalexander]

Just simply loved it ❤

[@shomusbiologyofficial]

Thank you

[@chintanmehta4695]

Superb sir.

[@shomusbiologyofficial]

Thank you

[@aayushisingh5651]

Thankyou sir❤

[@shomusbiologyofficial]

You're welcome

[@farhanakhatoon26]

Thanku so much!😃

[@shomusbiologyofficial]

+Farhana Khatoon glad you liked it

[@anastasia9370]

thank you so much for this

[@shomusbiologyofficial]

You're welcome

[@martinaschembri7933]

thank you so much!

[@Moongrace-sj4fc]

You are a blessing shomu . Please make a video on production of biomass and primary, secondary metabolites please🥰🥰

[@shomusbiologyofficial]

Okay. Thank you

[@Moongrace-sj4fc]

@@shomusbiologyofficial your welcome 😄

[@jyotiraj9362]

sir please make a video of strain improvement

[@shomusbiologyofficial]

+Jyoti Raj okay. I will Keppra that in mind

[@lubabahnuman3227]

great job

[@shomusbiologyofficial]

Thank you

[@RohitKhajuriaa]

awesome out of the world

[@aynuraydin6991]

Thank you for your video!!!

[@shomusbiologyofficial]

You're welcome

[@ithirstyforknowledge]

Nicely explained. A question: the recombinant plasmid will multiply to generate more similar or without the gene? And why?

[@patriciajimenez7443]

Hi, what is the process for extracting these recombinant proteins from the E.coli cell?

[@patriciajimenez7443]

Also, thanks for this video. This one is particular has been so helpful in so many levels!

[@crownofall]

I need this answer also.

[@HelloAayush]

Downstreaming is purification and separation process.

[@itsnotme8554]

HIV test kit have pre-coated antigens. So during the testing means pouring the blood drop from finger to test kit if we touch the dropper end to the test kit so can antigen go to our body and can give infection?

[@geraldomedeiros5081]

Excelent video!!! By the way, what is the gene that encodes human insulin for type 1 diabetes?

[@shomusbiologyofficial]

INS gene located on the short arm of human chromosome no. 11 at position 15.5

[@hamedhosseini4938]

Please make a video about GMO's.

[@shomusbiologyofficial]

+Hamed H I will make that soon.

[@hamedhosseini4938]

Ty

[@hamedhosseini4938]

Bro where is GMO? ;n;

[@crownofall]

I feel i need more details so I'll look at your other vids.

[@apurva2171]

hello , i got a question somewhere why is recombinant protein cloned before tag sequencing ?

[@khaledalharmoudi3350]

Thank you. helped me a lot.

[@shomusbiologyofficial]

You're welcome

[@saiky2925]

Thanks, legend

[@shomusbiologyofficial]

You're welcome

[@MANISHKUMAR-rz5fv]

Please upload the video on protine engineering 🙏🙏

[@shomusbiologyofficial]

Sure

[@cvhashim]

Thanks man

[@shomusbiologyofficial]

+cvhashim thank you

[@1307paolo]

Thanks!

[@ganguliify]

great.....keep it up

[@antarachaukidar2617]

Is this applicable for Protein engineering too?

[@ashokapalihawadana6407]

thank you so much

[@shomusbiologyofficial]

You're welcome

[@MomoTV]

thank you

[@prashantkumar409]

Shomu make a vedio on fusion protein.... plzzz

[@shomusbiologyofficial]

Okay

[@nunitchagucci3047]

You are awesome! Always! :)

[@shomusbiologyofficial]

+nunitcha gucci thank you.

[@deepikak.p5903]

Sir can you say about heterologous protein in brief .

[@Lumax96]

Hi, I've just got a question to see if I understand: so the DNA that is being used is called recombinant DNA because its different from the naturally occuring DNA, namely because its a recombination of a bacterial plasmid and a eukaryotic gene. But the protein that is being produced this way does not differ from the naturally occuring protein, but is called recombinant protein because it was produced using the recombinant DNA. Is that correct?

[@techheaven007]

yes

[@markboccanfuso2871]

So this can be used for bad as well as good

[@BE74297]

ya more creepy control science, cause of all the problems

[@jyotiraj9362]

sir after 3 days my exam will be started so plz make this video....

[@NH-jl2nb]

very nice

[@shomusbiologyofficial]

Thank you

[@divyaarora_5681]

Is it same as chimeric proteins??

[@thedevilsadvocate5210]

How do they collect the desired protein?

[@fadhlalshaebi2314]

Thanks

[@shomusbiologyofficial]

You're welcome

[@fadhlalshaebi2314]

i need recombinant the Il-34 protein and amplification it in the Lab. i ask if its possible. Thank so much

[@rajeshr4115]

What is the difference between Biologics and biosimillar production one video upload bro

[@Naveed_14]

Recombinant Protein Expression in E. coli (Inducible Promoter System)

[@shomusbiologyofficial]

Okay

[@abbasi1907]

Recombinant antigens are virulent or non virulents ?

[@zahrabatool7761]

Please give detail video

[@lovepunjab5429]

As we know plasmid is undergoes self replication , then why we place it into bacterial cell . Why we cannot give some chemical to it out side thy body _in laboratory??

[@leroyjenkins3375]

But how is the Plasmid inserted into the Bacterial Cell??

[@muthyalakapil8743]

Inserted with the help some physical methods like micro injection electroporation, some chemicals

[@ShubhamSingh-gr3is]

How recombinant proteins is recovered from host cell

[@chem337]

❤❤❤

[@shomusbiologyofficial]

Thank you

[@susanazizi3890]

In german we say : bester Mann

[@muthyalakapil8743]

Thank you so much sir... please make a video on chromosome jumping.. please sir ..

[@shomusbiologyofficial]

Will make it soon

[@deepalipandey829]

You look so good😍❤️

[@shomusbiologyofficial]

Thanks

[@Swatikapoor...]

Is this also called protein engineering

[@shomusbiologyofficial]

Yes

[@Nhthuyanh]

Hoping my biotech final will go well

[@shomusbiologyofficial]

All the best for the exam

[@HelloAayush]

👍

[@Patelpawancool]

Your video's super. But. I think. At last the video u summerise whole video in few Ward's. Do this reason user get easily. Motive your videos

[@shomusbiologyofficial]

That's a good idea

[@rijilfreekdaredevil]

Thank you bro🌹

[@shomusbiologyofficial]

You're welcome

[@nishakashyap1352]

🙂thanku sir

[@shomusbiologyofficial]

You're welcome

[@ashutoshthakur7712]

Bhai sahab...Angreji achi hai....Nhi Nhi. bohot achi hai... please thoda biology ka bhi content badhaiye....Baaki aapk angreji ka assent acha hai

[@shomusbiologyofficial]

Biology ka content kam laga??

[@wasimsalafi]

And now because of "go rakshaks" one cannot count on cows to extarct recombinant insulin in India ;)

[@shomusbiologyofficial]

We no longer use cow. We use bacteria now. It's much better and faster. Use recombinat dna technology

[@wasimsalafi]

@@shomusbiologyofficial hi Shomu, in case you didn't get my joke, i was kidding. Btw nice explanation of cloning.

[@kamranhaider5108]

We tolerate u in ur videos

[@lyndabelhadi7787]

Thanks

[@shomusbiologyofficial]

You're welcome