Revive Your Old Seeds: How to Germinate Seeds Using Hydrogen Peroxide!

[@timbabila]

Thanks mate, i used this on some old white sage seeds and one of them sprouted already.🎉

[@PlantCellTechnology]

Amazing!! Thanks for sharing your update with us!

[@timbabila]

@@PlantCellTechnology 2 of 6 came out eventually but i saw in some artikle that they are tricky to sprout. I already made 2 cuttings and rooted them in tap water placing them in a window where the sun comes up and they only get morning sun.

[@PlantCellTechnology]

That's great. At least you succeded in getting two of them to sprout@@timbabila

[@ianyoung8392]

- number seed packets and test tubes appropriately - 10ml 3% hydrogen peroxide +190ml of distilled water + 1g of sugar - shake for 10 minutes? - seeds soak 24/48 hours in the freeform solution - carefully decanter liquid with partially opened lid - move seeds with sterilised tweezers - place seeds in tissue culture or soaked towels before transplanting to soil if growth isn't great? Questions, the test tube lids are air tight and the only oxygen is from the hydrogen peroxide? The water used for the seeds, simply discard each time or is there a better way of recycling the infused water? Size of tubes used? If you use a pressure cooker to steralise your tubes in the rack do they need to be a particular type?

[@boblossie3192]

This is all GREAT information. I especially like that you broke the formula down to Ounces and Tablespoons. I multiplied that by 5 and made a batch that would just about fit in a 48oz glass bottle. Then I use that bottle to pour over the seeds to soak in 4 different jars and store the remainder of the mixture in a cabinet away form ALL light. THANK YOU FOR MAKING THIS SO EASY! AND I love that you mentioned it's a good practice for old AND new seeds.

[@PlantCellTechnology]

Thank you so much. We try our level best to make tissue culture information easy and accessible for our culturists. Happy that it's working for you all.

[@XxemoX4lifex]

I'm gonna try this. I'm having trouble with older baker creek pepper seeds. A few years old and 0% germination.. frustrating because they're not cheap seeds

[@PlantCellTechnology]

Go for it!

[@SpaceH3r0]

Great video! I personally use honey instead of sugar. Its harder to work with because of the viscosity and it can be difficult to dissolve but it has trace minerals that refined sugar lacks.

[@PlantCellTechnology]

So, how are experiments going for you? are the plants doing well? We would love to hear more about it.

[@SpaceH3r0]

@@PlantCellTechnology So far plants do well with this. My current projects are some rose bush seeds, red osier dogwood seeds and colorado blue spruce seeds. All 3 varieties are old seeds and require scarification and stratification. Im hopeful that using this method with honey will yield good results. As of writing this msg, all three varieties have gotten the full treatment and have been currently sitting in the fridge for about two weeks now. I will say that a lot of seeds have swelled already which is a good indication. In about 2-3 more weeks I expect to see some germination hopefully. I will keep you posted.

[@PlantCellTechnology]

@@SpaceH3r0 This is wonderful. Thanks for sharing with us!

[@ColdSun87]

Great video! In the cannabis industry there are tons of people using carbonated water to germinate old seeds. Have you ever used this method and can you explain how it would help in assisting germination of older seeds??

[@PlantCellTechnology]

We haven't tried the method on Cannabis yet. Francisco only works with houseplants, carnivorous plants, and desert plants. But, you can check it by yourself, as the method doesn't require much time and involves only a few ingredients. :)

[@salihkayadibi9023]

İ am trying right now other wise seeds will be wasted

[@snuffy166]

@@salihkayadibi9023 did this work for your cannabis seeds? i got some old seeds I can't get to sprout

[@r3dhawk3]

i like the added freedom units

[@CitizenJourno-1]

Have you run a side-by-side with him without the sugar? My first impression would be that all the energy for germination is in the seed itself. Cheers

[@PlantCellTechnology]

Yes, but best results with sugar.

[@VeggieBeard]

I have seen and used H2O2 for the scarification of seeds. I have not left them until they germinated. I will have to give that a try. Shave days or weeks off germination would be excellent, especially for peppers in zones with a short season and limited space indoors to start early. I am curious, though, what was in the cups he transferred the sprouted seeds to. Was that just water, or was that also the solution they sprouted in. If it was just water he transferred them to, would the solution harm the sprouted seedlings if they were left in it. In the second video, these questions are still not answered either. Please clarify. Thanks.

[@VeggieBeard]

Apparently, I did not understand him at some point. I believe he said that as he was removing the seeds from the sprouting solution, he was placing them in tissue culture.

[@PlantCellTechnology]

The germinated seeds were transferred to a suitable tissue culture media.

[@BasicPoke]

Did you say "freedom units" at 1:15?

[@PlantCellTechnology]

yes, that's right!

[@Reverse-generic-engineer]

Looking nice bro

[@PlantCellTechnology]

Thanks!

[@danieljheelan5256]

Hi Francisco, Hope you and your team are fine? Thanks for sharing. I use this method for orchids seeds. What you do if you get contaminations in your flasks due to seeds sterilization. You sterilize again? Thanks for your reply. Daniel Jheelan

[@PlantCellTechnology]

Thanks so much! Resterilization would be good for the plants that were in the same container as those contaminated. But, if they are already contaminated, then its recommended to get rid of them instead of sterilizing.

[@brendabennett4196]

❤thank You for sharing this

[@PlantCellTechnology]

Happy to know that you liked it!

[@dingo5842]

What is the original concentration of H2O2 prior to dilution? I have seen 35% food grade, 6% pharmaceutical grade, and common 3%. For whatever reason, your video doesn't specify (but it's very important to know). Thank you for making this video.

[@PlantCellTechnology]

Hi, We will confirm it with Francisco and let you know.

[@dingo5842]

@@PlantCellTechnology Thank you. I'm so glad I found this channel!

[@PlantCellTechnology]

@@dingo5842 Glad, you found our channel helpful! Also, waiting on Francisco's response. Will update you as soon as we get the answers.

[@rivercrockett343]

Start watching (listening) at 1:00. He mentions 3% hydrogen peroxide. It goes very fast, easy to miss that.

[@himadityachoubisa295]

​@@PlantCellTechnology I am sorry to say that you are incorrect here. It is narrated in the video to dilute 3 % solution further in 1: 19 ratio.

[@TheDuckofDoom.]

What type of gel media, are there any PGRs? I have seen GA3 used to help germination but that was a seed soak, similar to the peroxide, not used during and after germination.

[@PlantCellTechnology]

Hormone-free MS media

[@deepakbhartia8565]

How many days can we keep the seeds in this solution?

[@PlantCellTechnology]

Until you see them sprouting. Like small radicles coming our of it.

[@ne1cangrow603]

Depends on the kind of seeds, delicate medicinal I have found best results with 24 to 48/hrs. The secret sauce is genetics/temperature and timing, don’t let it stay submerged once the reticule emerges, before it pops open is best to remove from solution👍🏻

[@NorthernThaiGardenGuy]

I have 50 year old Marigold seeds that belonged to my Mom. Will this process work for them and if so, do the times and measurements still remain the same for soaking?

[@PlantCellTechnology]

Yes, it would be the same process. However, we can not guarantee germination.

[@taylorgraham842]

I see you transfer these into tissue culture but we never see you sterilize the seeds or make sure that they are clean before putting them in-vitro. Did you not have any contamination from this?

[@PlantCellTechnology]

No, we don't have any contamination. Hydrogen peroxide itself acts as a sterilant, so after sprouting, we directly transfer it to the tissue culture media.

[@indianb1918]

Can you add Gibberellic acid acid to hydrogen peroxide solution and if so how many grams of Gibberellic acidd powder to mix to the hydrogen peroxide solution ?

[@PlantCellTechnology]

Depends on what you want to achieve with that. The protocol we shared works great for the germination of seeds. We didn't try adding different hormones or chemicals. But, we'll let you know if we perform any such experiment.

[@indianb1918]

@@PlantCellTechnology Thanks a lot for such a useful video.

[@_FERBONSAI]

Hello, I want to put it into practice with adenium obesum seeds. After sprouting them in the mixture; 10 ml H2O2 at 3%, 190 ml distilled water and 1g sugar, (tell me if the proportions are correct), I want to transfer them to crop soil once germinated. Can I wipe them directly or do I rinse them first to avoid fungus? I say this because of the sugar residue that may remain attached to the seeds. Thank you so much.

[@PlantCellTechnology]

Can rinse the seeds with water or more hydrogen peroxide.

[@_FERBONSAI]

@@PlantCellTechnology Le agradezco de corazón la información que me proporciona. Gracias y saludos.

[@PlantCellTechnology]

@@_FERBONSAI Happy to help!

[@wshark23]

Great Video! I had one question, would this method work for very small seeds? I'm about to sow some old begonia seeds but the seeds are very small. They are about 2-9mm big and I wanted to see if this would work since I have mixed results with germinating in the past

[@PlantCellTechnology]

Absolutely! Our students have done this method with several different sizes of seeds, even like the begonias! Definitely give it a try!

[@wshark23]

@@PlantCellTechnology Awesome, thanks for the help

[@neilbennett9281]

How did you do with Rocoto or Carolina Reaper. Seeds that take a long time to germinate?

[@MrFatalfurry]

Question Can i put the seeds in the seedling tray into the medium and then do this procedure?

[@PlantCellTechnology]

No, you want to soak the seeds first and then plant them.

[@hariatmakhalsa1648]

Thank you for your expertise on sprouting seeds! Where can I get the test tubes? I have morning glory sprouting. It looks like you lay the sprouted seeds on top of the soil. Do I do this with all , any seed?

[@PlantCellTechnology]

you can purchase them from our website: plantcelltechnology.com/collections/all

[@vivekn7797]

What is the strength of the hydrogen peroxide solution?

[@PlantCellTechnology]

it's 3% H2O2, diluted in 19:1 that will be 19 parts water and 1 part h2o2.

[@rafaelrosa4988]

Why so diluted? Have you ever tried 1% solution + sugar?

[@berniefisher9472]

Thank you so much. Can i use rain water instead of distilled water. Am going to try it on my foxtail palm seeds.

[@PlantCellTechnology]

No. You can't use rain water or even normal water. You need to use distilled water in tissue culture applications.

[@GTMamaBear]

Would this work with dried tangerine seeds? I saved some seeds from my Grandparent's tangerine tree in 2020 at the last funeral. We grew up eating the tangerines straight from their tree. We are starting a homestead in June and I would like to try to plant them.

[@PlantCellTechnology]

This has worked with all the seeds we have worked with, so it should work. You can first try it with a couple of seeds to see how it goes and then try it on a larger batch.

[@nocapitals9833]

it is hard to understand you, but it sounds like you said 1TBS of H202, 2 ounces of H2O and 1/4 TSP of sugar. this isn't a 1:19 ratio because a tablespoon equals an ounce.

[@BasicPoke]

One US ounce is 2 tablespoons

[@nocapitals9833]

thank you for that@@BasicPoke

[@peterdickson2335]

Is this method applicable to oil palm seeds and coconut?

[@PlantCellTechnology]

We didn't try the method with those seeds as we didn't have any access to them, however, you can give it a try yourself. The method only requires water, hydrogen peroxide, and sugar.

[@johnbrightenstine837]

Could you or "should you" use unsulfured molasses instead of sugar for the micro nutrients in it? Thanks.

[@PlantCellTechnology]

That's something we haven't tested. However, we don't think it is a good idea because molasses will stick to the seeds and will lead to contamination.

[@carlosbautista-alvarez6759]

If seeds aren’t old can we still use this formula?

[@PlantCellTechnology]

Sure. Give it a try. It just requires three ingredients,: water, hydrogen peroxide, and sugar.

[@donnah.7346]

I have well water. If I boil, sterilize, and cool the water would that be sufficient instead of distilled water? My water is very hard with lots of minerals in it. What are your thoughts? Is it bad to spray the seeds as they grow with your solution? I want to grow hydroponically. I know odors can occur. I've seen others use hydrogen peroxide as they grow too. I plan on changing the solution daily.

[@PlantCellTechnology]

Boiling the water will not remove the solids. Now, if you are growing plants in hydroponics, the water needs to be treated anyway. Hard water with lots of minerals is usually not desired in hydroponics, as the minerals will unbalance the water and nutrients.

[@donnah.7346]

I'm only growing wheat grass. I did boil my water. It's slow going but it's growing. I did soak it with hydrogen peroxide and added sugar like you did. I think at least 70% of the seeds started. I used another formula of 1 1/2 tsp of hydrogen peroxide to 1 cup water with the sugar. I think I soaked them for about 32 hours. I also rinsed them in the sink twice daily with warm water. No mold or nasties showing. No bad smells either.

[@PlantCellTechnology]

@@donnah.7346 That's great then! right.

[@donnah.7346]

@@PlantCellTechnology Yes, your ideas and video were very helpful. I'm wondering if I should get some liquid hydroponic plant food. I'd only need a cup or two of water. Trying to figure out how much to use will be my problem. I hope they have good instructions. I will probably only need a few drops.

[@PlantCellTechnology]

@@donnah.7346@donnah.7346 Yes, and if there are no instructions with the product. You can always contact the company on social media (if they are there) or email them with your inquiry. We hope this tip helps!

[@shogunhoneybear]

What's the max amount of time you would leave them in this mix?

[@PlantCellTechnology]

Until they sprout.

[@MarlaWilson-m1w]

I have a few packets of seeds from 1974 and 1977. Beets, cabbage,carrots one other I think radishes. Would love some advice

[@PlantCellTechnology]

Hi, what advice are you looking for specifically?

[@TheStrutz87]

Could honey be used as a sugar source?

[@PlantCellTechnology]

Better to go for table sugar.

[@dineshbalkissoon7693]

Hi what kind of sugar to use brown or white

[@PlantCellTechnology]

You can even use normal table sugar.

[@medelle2014]

Hi, great video! I have question about disinfecting the seeds. I use 15min soaking using 10% bleach + soap solution for disinfecting seeds now. (I heard that is the standard method?) I mainly do this because my batch of seeds have high probability of fungi development. Would hydrogen peroxide do both disinfecting seeds and revitalizing them? Or, should I do my normal method of disinfection and hydrogen peroxide treatment later?

[@adctex]

Please reply to this question regarding disinfecting the seeds..... thanks.

[@ne1cangrow603]

🤷🏼‍♂️🤷🏼‍♂️🤷🏼‍♂️🤷🏼‍♂️

[@JoRoBoYo]

aren't hydrogen peroxide a type of bleach?

[@donnah.7346]

@@JoRoBoYo Sort of but totally different chemicals. If you use bleach you probably will kill the seeds. Don't use bleach.

[@hariatmakhalsa1648]

Is distilled water important?

[@PlantCellTechnology]

Yes, for tissue culture. Especially if you are doing it for commercial use.

[@jeffreyMagnum460]

Is rain water ok to use?

[@ghostdanceglass7975]

No rain water or tap.Too many contaminates.Distilled, reverse osmosis should work.

[@charliemcgriff7643]

Hey friend does that method works for collards seeds?

[@PlantCellTechnology]

We didn't try the method with those seeds , however, you can give it a try yourself. The method only requires water, hydrogen peroxide, and sugar.

[@oliverchu7240]

Hi i have soaking the seed the same solution for 48hr already but some seed is pop up the root but some are not , Because some the seed that i have it 10 year old , what do you recommend for the seed that not pop up and been soaking for 48hr ? Do i need to soak it more or put in the paper towel better ? and some of the tube that i have soak the color has change too , Do i need to change a new soak solution ?

[@oliverchu7240]

@PlantCellTechnology

[@oliverchu7240]

⁠

[@oliverchu7240]

@PlantCellTechnology

[@PlantCellTechnology]

Hi, We heard you and forwarded the question to Francisco because only he can answer this better. We will let you know as soon as he responds. Appreciate your patience.

[@oliverchu7240]

@@PlantCellTechnology thank you , i will wait for Francisco answer then

[@charliemcgriff7643]

Hey friend will it work for 4 or 5 years old collards greens

[@PlantCellTechnology]

We didn't try the method with those seeds as we didn't have any access to them, however, you can give it a try yourself. The method only requires water, hydrogen peroxide, and sugar.

[@poppyred7411]

It seems that you didn't rinse the seed or germinated seeds with water after soaked in H2O2, correct?

[@PlantCellTechnology]

Yes, that's correct.

[@database4074]

I have Hybrid onion seed expired in 2019, should I try?

[@PlantCellTechnology]

Absolutely. Just go for it.

[@krazykitty5750]

So 190 ml equals 6.43 oz, distilled water, correct? Ill be doing some rare seeds, expensive! This was suggested by the grower. 😊thanks for the guidance 😊

[@PlantCellTechnology]

That's absolutely amazing! and, yes 190 milliliters is approximately equal to 6.42 oz.

[@chariefemansfield3868]

Hi , I’m Jason from Australia I’ve been watching your videos and subscribe to your channel, but I’m still haven’t found anything regarding media and technical for cymbidium orchid green or dry pod plus mericlone with polyploid added colchicine or oryzalin to media . But most of the flask media in Australia that I have seen added charcoal. It would greatly appreciated if there was videos covering these topics, as I’m in learning stage of doing my own flasking with a still air box with basic equipment.

[@PlantCellTechnology]

Hi, Thanks for watching our videos. We'll definitely try to bring videos to your suggestions. So, stay tuned!

[@danieljheelan5256]

Hi Jason, I use 3% H2O2 for 5mn with dry orchids seeds. Butter to work with green pod as you can yse bleach to sterilize outside and without damaging the seeds. Daniel Jheelan from France

[@plantsandgreenswithelicia]

Hey Francisco, love the video. Question, did you use your general protocol for the peppers?

[@PlantCellTechnology]

Hi Elicia, This is with hydrogen peroxide. Which general protocol are you referring to?

[@11111111samy]

This will work with old strawberries seeds?

[@PlantCellTechnology]

We haven't tried the method with those seeds yet, however, you can give it a try yourself. The method only requires water, hydrogen peroxide, and sugar.

[@gmmo]

nice..

[@peterkando]

What strength hydrogen peroxide, please. I have 35% food grade. What is the mixing ratio, please 🙏

[@julianmichael2791]

Don’t take my word for it but read it was 2/3 start 1/3 hydrogen peroxide. What I did I just used water and sprinkled a very small amount of hydrogen peroxide into paper towel for a tiny little boost.

[@RRaucina]

Walmart 3% peroxide 6 bottles $6. best buy!

[@xaviercruz4763]

If tap or bottle water is used i also saw results in that the seeds sprout after a day or two days

[@PlantCellTechnology]

In tissue culture, you need to use sterilized distilled water as the tap contains some elements that can disturb the concentration of the elements in the media.

[@jayfeely7239]

Tap works for me every time. Some people get too excessive like using sterilized tweezers when non-sterilized tweezer will do the exact same thing. These things grow up in dirt. Stop it.

[@xaviercruz4763]

Yeah also check the date seeds from the time of Jesus Christ came to earth that sprouted and now the sell the fruit in israEL. It was thousands of years old and it sprouted!

[@1130MarsV]

​@@jayfeely7239It doesn't work like that. what you don't realize is that when you make a media with sugar and nutrients it is highly susceptible to mold and bacteria. Imagine fungus in an enclosed space with no competition and literally the perfect environment for it to thrive. If you leave food at room temperature it goes bad within hours and you will see nasty mold within days, it's the same thing. It would kill your plants. Potted plants aren't fed foods directly like TC plants that's the difference.

[@1130MarsV]

​@@jayfeely7239I guess you didn't see that he is talking about tissue culture

[@Sam9wilson9]

Solid!

[@PlantCellTechnology]

Thanks!

[@rafaelrosa4988]

Why so diluted? Have you ever tried 1% solution + sugar?

[@PlantCellTechnology]

That didn't work for us for the seeds we tried. We tested this method on many varities also, and it worked great.

[@majedalrajibi1409]

3% H2O2 ?

[@PlantCellTechnology]

We used 3% H2O2 in our previous video of "seed germination using H2O2". So, he is telling the combination is a bit different from that one.

[@NedDenver]

🔥🔥🔥🔥🔥🔥🔥🔥🔥🔥🔥

[@adctex]

I have several hundred 3-4 yr old daylily seeds. They have been in refrigerator for three or more years. Some I have been able to germinate, but most will turn moldy and not sprout. I want to try your method using the solution with the sugar in it to see it that improves the germination rate.... One other thing... PLEASE slow down on your speech in the videos... Try not to talk so fast as it is difficult to keep up and understand your presentation when you speak very fast :)

[@rivercrockett343]

You can tap your gear icon for settings and lower the playback speed. I think his (lovely) accent makes it hard to understand at normal speed.

[@PlantCellTechnology]

@@rivercrockett343 Right.

[@PlantCellTechnology]

@adctex Thanks for your suggestions. We will convey them to Francisco

[@adctex]

RE: Playback @@rivercrockett343 .... Playback speed is not adjustable when watching KZbin on Android TV boxes.. but yes, I can get on computer to do so, thanks for the reminder of that feature....

[@2lotsill]

Peroxide adds oxygen And sugar give nutrients. Done

[@PlantCellTechnology]

Perfect!

[@ASpinnerASpinner]

You say 1 part peroxide to 19 parts water, but in your older video you said 1 part peroxide to 9 parts water. kzbin.info/www/bejne/amPTaZ6Dbr2NfZYsi=jwvwamx8asvUhHYf&t=26 9 vs 19 is a very large discrepancy.

[@PlantCellTechnology]

It's 19:1 parts.

[@Topsrite]

It’s old, slow and have poor vision. Your video spun me into dizziness. I’m sure your content was good but I had difficulty watching

[@PlantCellTechnology]

You can adjust the speed of the video in the setting if it seems faster to you. It will help you analyze every step.

[@msgupta26]

Thank you for a very useful video. What is the percentage strength of hydrogen peroxide that you use? Is it 3% or 6%?

[@PlantCellTechnology]

It's 3% hydrogen peroxide

[@msgupta26]

@@PlantCellTechnology thank you very much.