Salting in and salting out | How does salting out happen? | what is salting in of proteins?

This video explains the concept behind salting in and salting out in details but within 5 minutes. this video will answer following questions
What is meant by salting out?
How does salting out happen?
What is salting out in protein separation?

[@ranjitshaw9110]

This is the best video for explaining the concept of Salting in and Salting out.... Thankyou dada for this....l would like to ask you about the reference where l can get the exact text of this explanation!!!!!

[@animatedbiologywitharpan]

There is no 1 proper source for it ....several review articles and text books . But you can follow voet and voet

[@animatedbiologywitharpan]

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[@ranjitshaw9110]

@@animatedbiologywitharpan Ok dada....l will try to look into them

[@ranjitshaw9110]

@@animatedbiologywitharpan Sure

[@ranjitshaw9110]

Is there any video of Parkinson's disease made by you?

[@shawnmakiri4435]

Thank you. You did something my textbook and my lecturer couldn't do.

[@skydivenext]

no is because is very boring why do I have to study stupid biochemistry for my degre what a joke bro lol

[@martaromeromarcos5816]

our teacher's explaining his subject with ur video, you can be proud bro

[@animatedbiologywitharpan]

Marta Romero Marcos please share my channel link with your friends and help me to reach big audience

[@marcosmartinandres710]

@@animatedbiologywitharpan She shared it with me!!! I've learnt a lot!

[@richardwells5390]

I don't understand though ... I understand most of it, but why does the salt start to react with water at higher concentrations. It's not as if the ionic interaction between the salt and the negatively charged residues isn't still stronger than the bond between the negative side of the polar water molecules and the positive salt. Intuitively, I'd think that the salt would first interact with the protein, and then when there isn't enough left, it would then react with the water. What am I missing. Can someone explain this to me? Other than that, I understand this.

[@argowal]

Great work, thank you for your presentation, it was clear and informative. the use of graphics greatly helps, keep it up! Would love more about protein separation and electric properties.

[@animatedbiologywitharpan]

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[@sh7arwt3teer99]

please if you can explain to me, why the mobile phases containing a concentration of ammonium sulfate promote the binding of many proteins to HIC columns??

[@manaswanimishra8395]

sir can you please tell me why and how high molecular weight has less surface area and that leads to less hydration less salt required for precipitaion of protein in case of globulin which is know as half saturation and which is exact opposite for albumin that ppt in full saturation

[@কৌশিককুন্ডু-ঝ১ত]

Electron microscopy confirmation of surface labeling of proteins

[@gigiarias7659]

Such a great refresher a year after my biochemistry courses! The graphics are really helpful, great work!

[@animatedbiologywitharpan]

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[@imdadmarwat3452]

sir plz give more detial how much saturated salt sol for specific purification which protocols is to follow for desire protiens

[@adosar7261]

Protein in pI hasn't net charge. So how "counter-ions" are around the protein in that case?

[@pieromg8587]

Thank you so much for making this fantastic video ❤

[@animatedbiologywitharpan]

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[@ThePerformanceConsultant]

Beautifully explained thank you! I've been struggling with sample preparation for months now - I have VERY expensive triple-labelled samples for NMR so, naturally, sample stability has been a great concern to ensure we can get the most out of each prep. Have you got any rough kind of buffer optimisation "workflow" that you go through when determining protein solubility? I've thus far tried crude hanging drop precipitate screens using a crystallographers microscope, as well as a thermal denaturation assay utilising a hydrophobic residue-binding dye which infers protein unfolding using qPCR (ThermoFluor I believe is the technique name). Have you got any other suggestions? I'm still struggling somewhat with long term stability.

[@flxmkr]

I just wanted to know if it's safe to salt steak on the grill. 🙂 Actually, I suspect I'm Fragile X positive, but I can't get the test. Insurance is ok with it. I just can't find a lab. So I'm thinking, "I have a microscope...and lancet...and leishman's dye...and salt." So I figured I could give myself the test, but it appears more complicated than I expected. I do that. I oversimplify things and then realize I should've gone to a good college in persuit of a PhD (dang dyscalcula!)...but I probably would've got lost on the way to college, anyway...(dang dysgeographia!) Oh! ... Nice presentation, though! 🙂❤️

[@animatedbiologywitharpan]

Ok i understand your pain

[@ducktape8520]

Great video! Just have a litte remark: At 1:52 you mark the carboxylate ion which is part of the protein peptide backbone. Instead you should have marked the carboxylate at the amino acid side chain which is exposed to the enviroment.

[@animatedbiologywitharpan]

Thanks for your suggestion 😊

[@sohanibhat1584]

Hello, very nice video. Please make animated videos for GC, HPLC ect. Also, Ammonium sulphate precipitation, connected to this video.

[@animatedbiologywitharpan]

Link for hplc kzbin.info/www/bejne/jKOYpWCZnK17fZY

[@zibamajidi3026]

very nice and simple, thank you

[@animatedbiologywitharpan]

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[@strikefreedom7575]

Helps a lot! Thanks!

[@animatedbiologywitharpan]

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[@mohiniahuja3923]

I could not see the precipitation of protein in that graphic! Kindly use relevant and self-explainatory graphics in future.

[@animatedbiologywitharpan]

Thanks for your criticism. We would try to improvise in future

[@walakirajude3529]

The video is useful, thank you

[@mailkakhan2314]

Plzz help me i am not getting plasma proteins seperation in physiology

[@subbud2120]

I can understood so easily, Very good explanation sir

[@animatedbiologywitharpan]

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[@alandbestwn1055]

Thank you,You understood me very well،Keep making more videos🌹🤍

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[@eishakhalid2927]

THANK YOU, my prof sucks:((((( and this helped me so much

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[@gisellvm3170]

Very good explanation.Thank you very much!!!!!

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[@abhijeetsurwase9665]

Really useful video. Such a tricky concept made like easiest thank you 👌🏻

[@animatedbiologywitharpan]

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[@krupanidhi912]

Ammonium sulphate precipitation follows the procedure explained by you. It is the good and simple method instead of ultracentrifugation and other methods. Nicely explained. Continue.

[@animatedbiologywitharpan]

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[@kantamaniveeravenkatanaray232]

Please requesting you send the procedure to me

[@joyofliving5352]

Great bro. Could you talk about why Lanthanum.Cl3.H20 (anhydrase) precipitate in room temperature water? (it has low solubility for sure). This question has nothing to do with protein solubility.

[@danahhamad8088]

I was struggling with this concept but you sir explained it very well. Thank you 🙏🏻

[@animatedbiologywitharpan]

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[@SuperPuddingFish]

Short and clear, thank you!

[@rebeccaschill4880]

BLESS U BRO ,SERIOUSLY 🥺😭u saved my ass in 12 hours i have my exam and this made me finally understand 💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜

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[@shalaka6502]

Thanks alot for the video my concepts are Cleared very well.

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[@ilhemedu31]

I think with high molucal weight..we in are in case water in molecule. When the molecule is very low weigheit we are in case in molecule in water.. I think in hight salt the high molecule began to contract and push water in outside. We can see that in milk coagulation by elevating temperature.. I think all high molecular product can forme an emulsion Water in oil or oil in water.. Thank you for video..👍👍🇩🇿🇩🇿

[@Anci3ntDice]

Very useful thank you

[@fashionlover7650]

I was also salt out like protein by my ex 😢

[@animatedbiologywitharpan]

ha ha ha a

[@millykunda4537]

You are legit making biochemistry easy for me 🙌

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[@irfanfaridi6281]

So its basically hydrophobic interaction in salting out

[@megnareddy9499]

Thank you!!! This video was very well explained and helped me understand something even my professor could not help with

[@animatedbiologywitharpan]

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[@조하성-p2d]

Best explanation for salting in and out. I want to cite this reference Haha. Thank you so much

[@animatedbiologywitharpan]

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[@조하성-p2d]

​@animatedbiologywitharpan I am really interested in your video! Could you let me know more about the detailed mechanism of the Salting-in step? From your video, I understand that adding salt ions can make the structure of the protein stable along with reducing of the intermolecular replusion of the protein. Then, what is the mechanism of the water molecular can form the hydrate shell around by the protein? Is hydrogen bonding on the hydrophilic regions of the protein? Thank you!!!!

[@a3ministry997]

Thank you ! The concept is clear for me now😊

[@animatedbiologywitharpan]

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[@NaderSharif-p8p]

Great work buddy keep this up

[@animatedbiologywitharpan]

thanks a lot

[@sakshishukla03]

Thank you very much This was very helpful 👍

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[@pratibhamishra5370]

Thanks you so much dear , I have finally clear my concept with this video . Thanks to you once again .

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[@pratibhamishra5370]

@@animatedbiologywitharpan sir please make a video on water and their bond forming mechanism with biomolecules . Please sir this mine humble request to you.

[@elenaaltamiranotorres6762]

awesome! thanks

[@Hiiraatif]

it was an awesome video. you explained so well. thank you soooooo much

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[@hmh6040]

Thank you for your such nice and clear cartoon!

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[@ria7657]

finally it's crystal clear to me...thank you so much!

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please share among your friends

[@subhabratpanda3673]

Excellent video..m

[@animatedbiologywitharpan]

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[@MehediHasan-zc9dh]

It was very helpful and easy. Thanks

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[@lauramf1]

correct me if i'm wrong, but is it that with salting out there is a higher level of interaction between water molecules and the salt and lower interaction of the water with the proteins?

[@animatedbiologywitharpan]

You understood it correctly

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[@TanWeiJunDA]

nice explanation, thank you for explanation its very informative and helpful

[@TanWeiJunDA]

however i have a question, regarding the protein isolation, if i not sure about the solubility strength of my desire protein, then salting in (addding low concentration of salt solution to allow it to solube is better) or salting out is better. And base on explanation, i understand that both salting in and out is helping to isolate, is there any condition like protein that not likely to soluble will be favor for salting out process? if there is, is the example fibrous protein will be more easier to seperate in salting out or salting process? Thank you for answering, look foward to knowing more on this

[@animatedbiologywitharpan]

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[@rachaelo7523]

Great video! Are the counterions from the solvent solution?

[@animatedbiologywitharpan]

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[@animatedbiologywitharpan]

counterions are from the solvent

[@Gerolinger]

What a great video. Thank you very much

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[@rayaroy519]

Thank you for this lecture video. I have never understood this topic before and the mechanism behind it. This video has done it for me.

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[@tamilbiology5078]

Sir This concept comes under which topic in lehningers if u reply it would be useful nice teaching tq

[@animatedbiologywitharpan]

Protein purification

[@tamilbiology5078]

@@animatedbiologywitharpan tq so much

[@besttricksinfootball9146]

I appreciate your work sir thanks 😊

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Thank you YOU'RE THE BEST👍

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[@Nipandtalk]

Thank you for this great video! Could you make a video about pI calculations of polypeptide chains?

[@NaderSharif-p8p]

Great work buddy keep it up

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Great video, thank you so so much !

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[@prittamkumarsahoo273]

Maghia

[@aileneroque8320]

I understand salting in and salting out based on their definitions, but I don't understand why at 1:33 the definitions are placed on either side of the graph like they are? I would assume salting in would be in the left side, and salting out on the right? Edit: There is a clearer graph at 4:53 with the definitions on the "correct" side.

[@137bob3d]

you done good

[@tutalta4523]

I hope your are my doctor in university I’m second year in dentistry school and my doctor is just reading the slides without any picture at least

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[@tutalta4523]

@@animatedbiologywitharpan sure I will share it now

[@MariaChavez-ey5sr]

Our group just used your video and it was very informative about explaining the topic. Thank you!

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[@uvy3908]

I like your animations it helps give a clearer pictuce

[@anzosimon19]

Concept clearly explained❤

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[@Gabriela79830]

Thank you for this video!

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[@ElectricLoft]

This video neglects to show the chloride ion in salt, NaCl. The logic of the video no longer makes sense when the Cl- ions are imagined in the diagrams.

[@gloomyend1232]

@ 5:17 what are the blue circles surrounding the protein? Is it water molecule?

[@shubhamsaha6130]

Thanks ! I was looking for salting out mechanism in saponification. I hope the mechanism will be similar, as both protein and soap are polar. Thank you !!

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[@dcroko]

Awesome explanation. You did a finer job at explaining this concept than my professor. Thanks again.

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[@fatemehansari1855]

Finally got it thank you so much

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You saved my day within 6 mins🤗 Thank you!

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[@aishj1003]

Finally i understood..thanks 😅

[@joshuaemmanuel5823]

Does the same apply to dna as well?

[@animatedbiologywitharpan]

Theoretically yes

[@claraschmidt360]

Helps so much! Thank you

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Very helpful! Thank you!

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[@jenniejean-baptiste9467]

I usually have a hard time understanding how some of these processes work but you did an amazing job at explaining the process! I feel like I understand it and could explain how it works to another individual now. Thank you!

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Very helpful 🙂👍

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[@edvaldomaciel510]

Thank you so much for the explanation 🙏. Your wise way to explain alongside the illustration helped me in clarifying some points about the topic.

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[@thaislasar199]

Thank you!!!! great vid !!

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This was helpful, thank you

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very good ...thank you

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[@khadijakhan5290]

Very informative. Quick and easy to understand. Animation make it more clear.

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Amazing video!!!

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FINALLY, I understood this. Thanks so much!

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wowwwwww thank you so much!

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The video is really informative. 👍

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Nice explanation!👏

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best explanation ever!

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Thankyou🎉

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