Sandwich ELISA

[@hddggfdaa]

Wow।।। Awesome explanation with animation 😮

[@nurrahmanahmadsenoaji6048]

Thank you very much! It's great ! Very helpful!

[@ImranAhmad-df3mi]

#Superb 👍 sir

[@md.mahbuburrahman1777]

Explanation just outstanding..

[@mdrummanuzzaman]

Just best Like that: just like that. Very simple and relevant

[@Sara_2001]

The A antibody and the enzyne linked anti body is brought by the lab right? Not included with our patient antigen ?

[@ahmedeldesouky1878]

Thank You So Much !

[@sanishkumaryadav9837]

Please create videos on scintillation counter, gm counter

[@dewminihansika600]

Thank you so much

[@poonamjhamb5807]

Most helpful video 👏👏👏

[@bhoomikaa7361]

Thank u soo much, great teaching

[@abdullah.khan98]

Also known as dot blot Elisa !

[@NgBishal]

Alhamdulillah , its very helpful

[@ααυα-ω4χ]

Bro in one antigen howmany antibodies can Bind

[@bipinkb8519]

Only one, capture antibody will be coated to the plate, later antigen will be added, later detection antibody is added. The antigen forms sandwich between capture antibody and detection antibody. So only one antigen.

[@vangmayireddy9516]

Where is western and southern blotting?

[@shak11uk]

Thank you!!

[@hacerdogan8509]

thak you soo mch

[@akansha4575]

Thnkusomuch 😀

[@act_pvt]

Thank you for this

[@diligentupdates]

Best explanation ever😊

[@ααυα-ω4χ]

Bro best explanation..can u tell about direct elisa

[@aju6573]

Helpfull♥️

[@simransarkar4502]

But why are we using two antibodies, when we can work with only one antibody and link it with the enzyme. And when there was direct and indirect ELISA was there so what is the need for this one?

[@sajidmicrobiology]

Sandwich elisa is used to detect antigens where as indirect is used for detection of antibodies

[@simransarkar4502]

@@sajidmicrobiology sir we can detect the antigen using the direct one, so why to use this technique? That is my question.

[@sajidmicrobiology]

Sandwich ELISA generally has higher sensitivity and specificity than the direct ELISA because two antibodies are involved in capture and detection. Since antigen does not need to be purified prior to measurement, sandwich ELISA is a better choice for the analysis of complex samples than the direct ELISA

[@MirriamKatadzuka-x3p]

Thank you sir

[@armyg2369]

Well why cant we fix the enzyme linked ab in the very first phase? 😐😐

[@sajidmicrobiology]

Then reaction will give false poitive result

[@tanyanjanike6907]

Why are they called *Anti* A Antibodies. I don’t understand the Anti part

[@sajidmicrobiology]

Assuming that antibodies are specific to antigen named as A

[@tanyanjanike6907]

@@sajidmicrobiology thank you!

[@أصيلةصالح]

Thanks 💙💙💙

[@lonefarid7304]

Sir i want to know! How a single antigen binds to antibodies simultaneously in sandwich elisa

[@sajidmicrobiology]

A single antigen have multiple sites called as epitopes on its surface. These epitopes are the actual binding sites for antibodies

[@AdityaRaj-zk9eh]

thanks sir

[@janakachandrasekara2942]

Thankyou sir👍

[@martingeorge317]

1:35

[@lakshmankaranam]

Which method is used in labs ,for EIA combo hiv1/2 & p24 test

[@Theprofessionalsurgeon]

idk

[@tunetonic04]

❤❤

[@abhijithpramod2332]

Very useful for all.. understandable because good animation

[@rameshgorati7934]

Nc

[@SUNILSAHU-vq4fp]

Thank you sir for this topic

[@sajidmicrobiology]

Most welcome