Optimizing Human Hepatic Organoid Development: Leveraging iPSC Differentiation

Presenting a simple, standardized and robust workflow, where human iPSCs were successfully differentiated into hepatocytes using three distinct media types. Each media type was supplemented with specific growth factors and cytokines, at critical stages of differentiation.
This approach provided a relatively straightforward method for producing mature hepatic cells, which could then be utilized to generate human hepatic organoids. The method demonstrated a robust strategy for unlimited organoid development.
The presented workflow employed Sartorius RUO Growth Factors and Cytokines, in conjunction with the iQue®️ High-Throughput Screening (HTS) Cytometry Platform and an Incucyte®️ Live-Cell Analysis System.
The integrated approach facilitates the phenotypic characterization and monitoring of growth, simplifying the generation of functional hepatic cells and organoids from iPSCs. These resulting organoids are invaluable for drug discovery, development, and toxicity studies.
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Chapters:
0:13 Introduction to iPSCs and Their Importance
2:02 Understanding iPSC Derivation Techniques
5:54 Introduction to the iQue®️ High-Throughput Screening Cytometer
7:14 iPSC Differentiation Workflow for Hepatic Lineage
9:04 Results of iPSC Differentiation into Hepatic Cells
13:05 Functional Characterization of iPSC-Derived Hepatocytes
19:01 Impact of Growth Factors on Organoid Development
19:59 Conclusion and Further Resources
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