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SDS PAGE (Sodium Dodecyl Sulphate/Sulfate Polyacrylamide Gel Electrophoresis) is a routinely used technique for protein separation and analysis. It also serves as the gateway step for Western blotting to monitor protein expression. Using SDS-PAGE, we can monitor protein purification, estimate molecular weights of proteins, determine number of protein subunits in a multi-subunit protein and much more.