Simple trick for open sterile work without a flow hood!!

[@WillysWorld]

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[@jimsmij]

i love low tech diy for those of us that aren't planning on going big. thx!

[@vashstampede4878]

I use oven tek for my agar pours. Preheat to 250, crack it open and place a wire rack over the opening. Do your pours over the rack, good to go. Learned it from PGT. Thanks for the video Willy, good info for sure

[@kinzieconrad105]

I do mycology in the closet. No air current. I have pretty good results. With safe food handling practices. Bleach water spray bottle is my go to, sanitation for pennies, millet bird seed it the grain of choice you can get like a 30# bag for like $14, you can find damaged bags for like $3 at lowes and the depot stores.

[@benink5690]

Teach me!

[@MycolOG]

Would you like to start a channel and teach us lol

[@JustinLewis-k2k]

I use my 2nd wardrobe closet no clothes, storage products, no vents= no airflow- possible contam… I use the top shelf and got a few of the Boomer SHROOMER inflatable monotub. It took me a few weeks to find my groove getting my grains to not turn into contam. I barely go into my closet lab space, unless I’m working, and I take a shower and turn ac off and spray my gloves, forearms, and upper torso with 70% iso. Also I use a plastic tote that melted 8 holes the diameter of a Campbell soups can heated up red hot, and carefully apply pressure into the tote for the holes to be melted in perfect circles no drill debris of plastic shavings. I’m still learning but I finally got it down now. Next step I’m making my own DIY Mini laminar flow hood all for under $80

[@beauregardb6108]

Thanks! I like this, what I have done is take a similar sized bin, and wrapped shipping saran wrap around the top 2/3, leaving a wide hand port. I make it clean, but my bunsen into it for 10" and go. So far, so good.

[@jenniferwinstead6958]

Hah! I just saw this and I’ve been using my box exactly like this but I added an extra little safeguard. I added a cut piece of clear plastic tarp to cover the majority of the front part with a slit cut 1/2 way up so my arms can go inside freely.

[@alexn8795]

I've had great success(10 agar transfers and 4 grain transfers with no contamination) by using this sab method and I am happy you posted about it because everyone thinks im crazy for not cutting arm holes. I started doing it a few months ago because I realized during a smoke test that when I put my hand into a sab(through holes) air is sucked in and it does the same thing when you exit. The trick is you have to have the opening flush with the edge of your kitchen counter so you arm movements do not push air into the counter and into the sab. You also cannot have any airflow pointed towards the opening. I also sanitize my hands and arms every time I leave the sab even if its for a second. If I am working more than 15 min then I spray lysol into the bin. You can spray even if there is stuff in there as long as none of your plates or jars are open. I actually think this is more effective than the traditional SAB with arm holes. The armholes are great if you do not leave the sab until you are done but if you have to exit for any reason then when you re-enter you push air from outside into the sab(from air displacement) directly over your samples. If you are disciplined with your movements (always reach from below or level with your samples) and with sanitizing using the open SAB is more effective.

[@СквиртомирЯросветов]

Hello. How to sterilize a scalpel and an inoculation loop with an open fire in such a box? will fire create air movement or not? besides, it's flammable, because lysol contains alcohol, isn't it? Please help, I can't figure out this issue with the sterilization of a metal instruments.

[@alexn8795]

@@СквиртомирЯросветов I use a lab grade alcohol lamp too sterilize my tools. I do not keep the lamp in the SAB. I leave a few feet of open counter next to the SAB and sterilize it with alcohol and lysol before placing my lamp right next to the sab. Sterilize your instruments then while they are still red hot transition to working in the sab. Smooth but urgent movements are key to prevent contamination. Plan and practice everything you are going to do in the sab before you do it. This technique isn't perfect and there is still a risk of contam no matter how good you are. I have since upgraded my setup to a labrats laminar flow hood. Edit: let the lysol and alcohol settle/evaporate before you light anything outside the sab. There can be fumes inside the while you work but I always extinguish candle before I spray.

[@TheTubejunky]

It's been so long since I seen a Willy! Those other "content scalpers" just don't "MEASURE UP". Good to see you're still kicking it Myco!

[@OfficialCharlieElla]

Well done Willy. Thrilled to see you beat the pricks that shut you down some time ago.

[@necrophage5248]

I take a 10 gal aquarium, turn it on its side. Then I tape a towel to the top edge and two sides leaving the bottom with the slack of the towel.

[@philliprandazzo9834]

Willy is a real hero so much knowledge thank you Brother.

[@brothamyco9417]

he keeps it going

[@legion2k988]

So many people need to see things done to 'learn'. Reading about it is one thing.. seeing it with your explanation, I'm sure, helps a lot of people. Rock on!

[@brothamyco9417]

facts

[@dimemega00]

For your SAB holes all you have to do is put duct tape around the holes or glue a plastic tube around the holes to help your arms

[@McStreetz]

instead of cutting the holes find a metal can about the size of your arms, heat it up on the stove and burn the holes through. leaves a much smoother finish and a perfect circle with less chance of cracking the tub.

[@Letsjustbehonesttoday]

Homemade Flowhood under $30= 20×20 Box Fan with quality 20×20 Furnace Filter taped on the front. BAM Done!

[@kylebrowning88]

Hello, just starting out and wondering if this method still works for you and is still your go to? I tried it and got decent results but I am new and those results could be from any contam point. I switched to a SAB and seems to be about 1-2 out of 10 grain jars contam. But they did not contam on matched AGAR samples I kept. I intend to go back to Your Idea but try all over now that I have more knowledge than before. Thats why I was asking what you still think a year later. Is it worth it to use the homemade flow hood? Obvious there is way better but I mean on par with your statement.

[@Letsjustbehonesttoday]

@@kylebrowning88 Still standing strong, I just replace the filter from time to time. I even used that setup to vent my chamber after misting to promote Evaporation. Works great.

[@sugargraham6868]

Thanks Willy! Glad i ran across your video.

[@happygolucky1100]

Another idea to add is Saran Wrap across the front. Leave a opening at the bottom. Easy to get your arms in to work and easy to see through. Keeps the work space a little cleaner

[@JustinLewis-k2k]

@happygolucky1100 I’ve used this cheap very effective method without l the hassle of buying very expensive equipment that you don’t necessarily need but they are very good to have to eliminate altogether or reduce contamination

[@bradypenn1762]

This is what I do. About 2/3 of the top of the bid with Saran. Makes it so much easier to move around and see without having a the entire face open.

[@typerk]

I turn my unmodified sab upside down over the edge of my workspace and work under it

[@kevinshepherd6306]

A box

[@ENTREPRE-MOMMY]

Me too. Works great. A little annoying when inoculating a bunch of batches, but still works

[@jamesfowler7803]

Where did you get that hoodie? I love it. Thank you for all teaching brother!

[@WillysWorld]

Thank you! It was a gift from a TTF member that works for me. I just asked them and They bought it at Tillys

[@ANWCarpetCleaning]

I saw a trick where someone put their oven on very low and had a wire trey reaching out over a slightly open oven. The theory was that the rising heat created a somewhat laminar flow upwards and he poured his agar that way with the dishes on the wire trey. I have a sab so I don't need to do that. But it sounds like a good idea for broke boy tech. Haha. It would be cool to see some comparative results with these different techniques to see if one has less contam rates then the other.

[@Allaboutpie]

I've done that oven tech 4 times now making plates and slants and it works surprisingly well. I usually pour 4 cups of agar each time. I've been using the 2 ounce plastic condiment cups so I come out with quite a few. Probably about 50 but I usually do a fair amount of slants too which seem to have come out clean. Last pour I only have had one plastic cup with a little contamination so far. 25 cups I did of a separate syringe all came out dark brown everywhere the loop sneaked across the agar and it was fast. Second day I was throwing them away. The scary part was that I used the same loop for 4 different species one right after another. So we know that flame sterilizing does a excellent job.

[@rhondaspring2008]

Willy, show us your full grow set up.

[@mackb6537]

you redid the old vid! its so clear, amazing bro

[@David_Lo_Pan]

I always wanted to learn more about Mycology from a mortal kombat character, lol 🔮🙏🍄 Thank you for doing the Lord's work

[@MCH400]

Lmfao that's crazy I was watching this video looking at his mask for the first time and thinking "Damn the teeth kinda make him look like Baraka"

[@David_Lo_Pan]

@@MCH400 Two years later and I finally know now that I'm not alone lol

[@rufflesridges2334]

Always a good day when you see a new willy vid; Thank you man

[@brothamyco9417]

for real

[@Paulshuntz]

Bro looks like the nerd from breaking bad 😂😂😂

[@blestup]

I've only done it once right in front of the hot oven, advised by midwest. I got the kit from them. Have you heard of of oven method? After seeing this it does seem better than the oven method. I am going to do it my next go around. Anyway, you are a wealth of knowledge. I hope to be on the next zoom. headed over to P to see the next video now.

[@PageG111]

Yup I use a c-clap to keep rack secure. I learnt from Home Mycology. Taping a clear dry cleaning bag to small hepa fan works really nice as well. Makes huge sterile area to work

[@blestup]

@@PageG111 thanks!

[@PageG111]

Working over a cracked oven with a rack also works nicely, shout out to Home Mycology. Have also taped open end of clear dry cleaning bag to HEPA fan. Turn on and cut arm holes in side, balloons out creating huge sterile area to work

[@kindvine]

Thanks PageG.....seems I saw that tek on another site. Yeah those "dry cleaner bags"...that cover shirts/coats/etc. are really a great idea. Thanks again Page. Almost forgot about it....

[@benink5690]

"Cuts arms" omg that made me laugh. People with that little resolve might not make it in this hobby. The only reason I don't like a SAB is because I'm disabled from labor jobs due to my back. No worries I got a work from home job, as not working is the worst thing for anyone. The sab is horrible for posture and long sessions are not a viable option for me long term.

[@Houseofmycology]

Love the hoodie

[@petergrahammer1493]

I flame sterilyzed my SAB.. To much Alkohol...LOL🤣but i think is pretty clean👨‍🔬

[@lukemartin6927]

Lowes sells a small plastic greenhouse for 30 bucks. You put the frame together and slide the plastic shell over the top and cut 2 arm holes in it. Works great

[@ballpythonlover10]

Jesus that’s a great idea tbh I had about 4 from when I was going to do pans didn’t even think of that nice man

[@brothamyco9417]

brothamyco here showing some love 🛻🚨🚨🚨🚨🚨🚨

[@peteytwofinger]

found your channel today and i enjoyed watching you do your thing .

[@robinhoode9364]

Salute, Willy 🫡

[@slothmoths2372]

Willy Myco is the best TV show I’ve ever watched

[@brothamyco9417]

haha facts

[@Houseofmycology]

Willy can you sterilize the other plates that didn’t get used?

[@xj11w2c7]

These are some of the highest production value videos about this stuff.

[@vitas4895]

Thank you for your videos! It’s very interesting, you doing amazing job! Can you do video how to get 2nd and 3d flash for small commercial farm? I just started and can’t find how to do in KZbin. Not sure if I have to soak each bag for 12 hours? It’s a lot of work, is there is a way to do without soak in them and get 2-3 flashes?

[@bradxwx]

I never made a SAB. I took a tub and just turned it on its side and sprayed isopropyl alcohol on absolutely everything I touched and transferred to/from. Keep open flames away from that environment if you follow my lead. I've only had contam in a few agar cups. Transfers from agar to jars or jars to tubs have not had any contam. Not sure why only a few agar cups have contam and nothing else has. I suspect maybe syringes were contaminated?

[@Georges3009]

Funny how no one in the community seems to appreciate the open flame technique, which is btw the standard in academic microbiology. Typicaly it would require a bunsen burner and a source of gas, but I work with fondue fuel and the lil burner that comes along and i get pretty decent results, working in the sterility cone. I'll admit I'm using PastyWhite's no pour agar technique though.

[@Allaboutpie]

I was using an inoculation loop to start 4 different species on agar. After about 3 days I checked them and one species I had about 25 agar cups of was growing dark brown everywhere I streaked the agar plate with the inoculation loop. No white mycelium, just dark brown. The other 3 species have come out really clean and healthy. The ones that came out brown and contaminated where the first ones I did. Did all this in the same SAB Box, same batch of agar, with the same inoculation loop. If I didn't flame sterilize the scalpel, that would happened to everything. I only flame sterilized between each species. I really think the syringe is contaminated but I made more agar cups and put the spore solution on the agar directly from the syringe just to reduce as much contact as possible just to see if it happens again. Also flame sterilized the needle so I can hopefully narrow down what caused such an aggressive contamination. It took over 80 percent of the agar surface in just 2 days. It will be interesting to see if it happens the second time.

[@boatshoes7131]

Do you still do this and what are contam rates like? I’ve been thinking about just working close to my alcohol lamp but not a lot of info out there on this.

[@firedupfairy]

can't wait to try this method! Can I also make agar transfers this way?

[@davemutters3773]

Glad to have you back, happy you are feeling better, bro! You are amongst the best teachers I've had, Thank you.

[@SocialDeviant_]

Also I gorilla taped and cut a small cross with a razor blade into two sandwich bags over the arm holes which were just cut bigger so i didn't cut myself. This keeps my feom cutting myself and keeps air from getting in when i put my arms in and out... And i have done the same thing, but with clear industrial strength Saran wrap over the top half but tbh the alcohol lamp just worked better for me as long as you work close. You just need heat to push it all away. Also you can build a laminar for about 80-100 bucks its just the right fan behind a HEPA and enclosed, im even going to add some UV lights

[@Big-three492]

I use to grow mushrooms using the hydrogen peroxide method. Helps cut down on contam and can work in open air.

[@David_Lo_Pan]

H2O2 method?

[@Big-three492]

@@David_Lo_Pan adding 3% of peroxide to agar,hardwood spawn and sub. All done in open air.

[@AshikaUmanga]

few drops?

[@brothamyco9417]

OG

[@Stormephront]

Nice..

[@you-fq7ug]

You can make a flow hood for 40-100$. Only way to go. Either box fan with home filter taped to it. Or an eBay mounto hepa filter with an old squirrel cage and build a box. Hot glue works to seal shotty wood work 👍🏾go from 5-10% contam to 0. I put it in an ice shanty to recirculate clean air

[@ale.ktheo.g4394]

Is this better then making a candle circle around the working area?

[@bornforgains7903]

Dope video Willy, great knowledge!

[@AName147]

Can you just spray it with 70% alcohol instead of lysol or the bleach mixture?

[@cryptobuffet5311]

Peace Thank you for all your teachings

[@fruitsandflowersnorway620]

I remember you on a diff channel im sure… i remember yr vids being super detailed and good a back in the day . Now energy prices are bs worldwide recn mushies would be good to get goin

[@catchkennycruzin2268]

Notification gang

[@brothamyco9417]

haha

[@emeteriogomez3748]

I LOVE U willy

[@richsoul5437]

Our there any cultivation classes located near or around Oakland?! Willing to pay good money for hands on education/ skills

[@Steve4768o]

I'll refer you to this mate where I got mine delivered discreetly***¿¿¿

[@Steve4768o]

On Instagram..¿

[@Steve4768o]

Healerbryan2…

[@SocialDeviant_]

What about an alcohol lamp? It's cheap, easy, and you can diy

[@Grumpy303]

you could do this but tape a piece of clear plastic over the opening and put holes in that so it is a more flexible SAB

[@lisajohnson9768]

Genius. Thank you.

[@sewaddicted]

Can you use an air purifier in a small work room? Of course used with a sterilized work area.

[@SporeWhore661]

Use weather stripping and super glue for the arm holes 😉

[@DaveE99]

im still figuring stuff out, the kit i have had me innoculate jars in front of my oven at full blast with the door down and doing it right on the door it self or holding in front of it at least. does this not work for things like agar and syringe and prints i take it? just curious, as it would be the most convenient i feel.

[@oscormeyer2283]

Brand freaking new to the mycology world, found your video and will be getting started this week. Any tips for a first timer??

[@AudioStorm1980]

Agar...right off. Don't waste time with brf cakes....start bulk ASAP

[@gandalf2256]

Wear a condom

[@ZenAndPsychedelicHealingCenter]

This guy is not a good resource for mycology. You are better off with channels like Stipe & Cap, the Trusted Cultivator and sites such as the Shroomery.

[@alexc1105]

Put a clear plastic sheet as a curtain/drape to increase sterility!

[@psilosimon613]

Why is condensation bad on agar lids??? I'm going to try agar soon, thanks

[@toddhanis1516]

What's your thoughts on using charcoal filtration system instead flow hood? Unfortunately it is setup to recirculate. I'm going to exhaust it out window so it'll be negative air pressure? I'm novice/ beginner. Thank you for teaching me new things the correct way. I found new agar plates & ready bags with contamination 💩💔 seems like agar containers should snap r screw on 🤘😎🍄

[@robinhoode9364]

Positive flow from hepa filter

[@95_Nepentheses]

Carbon won't work for this. It does chemical filtration, removes molecular sized contaminants from the air. You need mechanical filtration, like a HEPA filter, to remove the larger bacteria/fungus spores from the air.

[@user-vq5hh9bk2m]

Is this as good as a still air box for purposes like innoculation, agar work, and transfers?

[@muddbutter]

But isn’t it being contaminated with the lid off while you’re pouring?

[@95manneya]

This is exactly what I did a week ago on a spawn to spawn transfer! Except I was also running 2 UVC w/ ozone lights inside the box. I’ve killed contamination with these lights. A must have!!

[@PageG111]

Please be careful with uv. My homie got wicked face/neck sunburn and couldn’t open his eyes for a day. He didn’t know it was on under a table, was out of sight. Had a remote and usually turned off hour before entering. He was in the room just over half hour and messed him up bad. Just be safe with them

[@seanmclemore7660]

So i got a question. So say i get some plates and they dont germinate because the agar was too dry when you inoculate what should you do would you want too try mist it with some kind of water?

[@gregavery3450]

Get it Lit Willy

[@e_j_r_]

Willy, what tier do I need to sub to on your Patreon to get access to the library??

[@theodorelutjen3135]

I did some petri dishes. All are gorgeous. One had a orange spot growing. I put methylene blue on the spot. It seems to killed the orange. Jack frost is doing great 👍

[@ScottYdo]

How long does it usually take for agar to solidify at room temp?

[@humongousfungus4759]

Thanks 🙏

[@psilosydetrusenses4125]

My agar plates always end up wet with clumps. What am I doing wrong

[@judyhowell7075]

Maybe let it cool some before pouring

[@RNAbarton]

Thank you so much!!!

[@bsarabia619]

Love it

[@brothamyco9417]

hes on it like a boss

[@danieldelgado1052]

I got some spores and put them to agar. The problem is they were delivered early and sat in the mailbox for 2 days. It is super hot here and I'm concerned that by not knowing they were here, I unknowingly screwed up and they probably aren't viable. Golden Teacher and Koh Samui are the strains. I am just learning from you and mycotrophic and I hate to think I wasted my $. Can you tell me your thoughts?

[@ryanwilhite]

Did the spores ever germinate?

[@openrealm]

Update.

[@positivevibesveda]

updates?

[@hanu9830]

is there any evidence of this guy actually growing mushrooms

[@MCT812]

GREAT!

[@pyroparrish2783]

That hoodie though

[@Jinsuun]

SAB is not a clean space and is not free of contaminants. Wth are you talking about homey.

[@Jinsuun]

And it's definitely not Sterile.

[@MrWizardjr9]

you probably wont get a 100% success rate but its better than nothing and you can get it pretty high if you are careful. a laminar flow hood is just too expensive for a lot of people

[@Jinsuun]

@@MrWizardjr9 I was just correcting his misinformation. Definitely not saying it's useless.

[@electrictrueform]

cracked ovens and open boxes..... shit you really figured this all out😒

[@shannonbrolin2436]

So you can use alcohol after using bleach in there?

[@doloinc]

You do an initial wipe down with a sanitizer, then coat the inside with a fine mist of water (ideally with very little soap) to trap particulates floating around in the box

[@MCH400]

I spent 15 bucks on temu and got this massive collapsible still air box where the sides zip and unzip and there are armholes with sleeves and elastic wrist cuffs and i do this exact method. I Lysol it, let it sit, put my shit inside and spray with lysol one mo' again and once its settled ill untie the sleeves, spray my hands andfet to work

[@ohhansel]

Willy Motormouth

[@cameronwilliams2246]

i use my left over plates for spore prints

[@bounzig]

Question: Why can't you sterilize the agar discs and use them again?

[@positivevibesveda]

This is 2mo late but I believe it’s because those containers can’t go into the pressure cooker. If they were PP5, you should reuse them.

[@bassman4201000]

Do you do consulting work? Oakland is wide open I may make an investment in the next 12 mo.

[@TheWastiddaze]

Do you need growers

[@TheWastiddaze]

I grow

[@joshuarossi7855]

I do reply if you want details

[@culinaryue9727]

Hey I got those same gloves… lol😂😂😂

[@MikeSmith-zv9tw]

I ❤ Willy

[@loyaltyisroyalty5616]

Just a tip for people that live in a newly constructed home: your house, most likely, doesn’t have enough contaminants built up to affect your grows. So you don’t even need a SAB or flow hood. Just do your sterile work in open air. But make sure you change your home air filters regularly and to turn off the HVAC system about an hour prior to working. Also, have good sanitation practices!

[@woody6791]

😂

[@geekay101]

Unfortunately, this is bad advice and even worse procedure. The air, wherever your room is, contains microbes of all kinds which cause contamination. Lysol is a disinfectant, not a steriliser, does not result in asceptic workspace, especially open to air movement like your open plastic box.

[@matthewgibson1953]

This should be filed under satire.

[@TroyKorody-yx7nn]

This ain't rocket science. Make it make sense. Dollar store pool noodle cut to circumference of the arm holes, then cut long ways to the center of the noodles. Place around the arm hole edges. Skin cutting Problem solved...

[@rickm1041]

Great videos butTake that Filter off you face man! I'm sure I'm not the only one that feels that way.

[@DangerRussDayZ6533]

FYI shroomery has long advised against using lysol because it is petroleum based and is believed to potentially cause mutations. Lots of people still use it, but I think bleach or 70% ISO is a better option. Remember, there's zero chance of actually creating a sterile environment like this, you're just trying to reduce the chances of potential contamination.

[@ballzzd33p29]

600$ pro guard air filter system. Cleans air with uvc light and carbon filter. Your welcome.

[@ianwade8499]

This dude is like peak intelligence bo from super store. He needs to airhorn outro. Bher bher bherrr!

[@OwlDeV1l]

🍄

[@SK-er2es]

Bro.... cut your videos down. You repeat yourself 20 times at the beginning of every video

[@RasMajnouni]

You need to add wide sunglasses, we KNOW who you are, the MASK...wait...are these videos the same ones i saw 3 years ago..you know Pre -Corona?

[@DanSetzer]

Put ur damn mask on