Empty vector is linear, which means that it cannot replicate inside E.coli. Normally vector cannot ligate onto itself because it is cut with different enzymes, so it has mismatching sticky ends. Sometimes vector digestion is incomplete, so it does ligate onto itself and replicates. This causes high background levels. That's why we have control plate when we do transformation.
@mahboob135919809 жыл бұрын
Thanks Andriy.
@K64JT11 жыл бұрын
Great! Thank you!
@samidastpeyman83410 жыл бұрын
Thanks. It is really useful and you explain well.
@CloningStrategies10 жыл бұрын
Thanks for watching ;)
@Sid953211 жыл бұрын
Great !!!!
@MityayKo11 жыл бұрын
Can you again please explain why we do not have colonies on the control plate, as you didn't use any antibiotics. Or the competence cells that you use do not grow without presence of the vector? Thank you.
@K64JT11 жыл бұрын
Just a little confused here. The vector has an antibiotic resistance gene right? So it empty vector is taken up it will still grow?