siRNA mechanism | short interfering RNA mediated gene silencing

[@jovialdyalsingh4106]

Suman, I have watched all the videos of rnai, you are a true genius Suman, God bless you always

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I have been following you from last years of my clg life, I'm a Micro stud and wanted to tell you that a lot and lot of students of NBU are crazy about you - it's like if we want to know any topic in deep ,we simply log in to you ...just to let you know that a hell lot of clg studs watch you and we talk about you and we all have seen the journey of your channel.... Thank you so much for being with us from our first yr to last one ❤

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PLEASE BECOME A PROFESSOR. I learn more from your channel then from my professors.

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+Scott Amon thank you. Glad you liked my lectures

[@nutribox4535]

agree

[@anamikamazumdar6147]

Totally agree.

[@thetravellingengineer3735]

All the 4 videos of RNAi are awesome, it cleared my doubts regarding RNAi, thank you Sir.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@periophthalmus3740]

I'm a med student and you helped me a lot about understanding the siRNA. Many thanks.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts to help

[@zunairakiran7636]

You are a competent teacher.. And know how to convey your information in easier ways.. Thank you

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[@mayukhroy5393]

Sir,You made me fall in love with Life Science.

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[@thio2620]

I am learning all my topics from one and only yours channel seriiously. First thing first that comes in mind when i have a problem in any topic is "shomus biology". Thank you so much. 😍 love from Pakistan! Stay blessed 🤞

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Thanks to you I am doing better in my QBM class at UCF.

[@pineapple4311]

4 years ago, when I began to look for jobs, I studied on your channel. Now I am looking to change my job, I studied on your channel again. Thank you!

[@shomusbiologyofficial]

You're welcome. I am glad to hear that you're getting benefit from my lectures

[@ramisharahman8813]

In your RNAi video you mentioned Argonaut to be RNAse type 3 but here you said Argonaut is RNAse H type. So which one is correct?

[@MindandAyurveda]

khub bhalo...sir ...this is the first time i am relying on any video lecture ...and its really fruitful.

[@shomusbiologyofficial]

+SHIPRA GOSWAMI glad I can help. Stay tuned

[@kirancu4713]

Sir, thank u very much you saved my time and made very easy to learn these things and watched your lectures more to study my molecular pharmacology subject.

[@shomusbiologyofficial]

Glad to hear that you are getting benefit from the videos

[@narayanyadav3132]

Highly admirable teaching approach Suman, Keep it up. Very easy to understand such a complex process particularly for a beginner, applauds!!!!!!!

[@swatichauhan2531]

THANK YOU SIR for teaching this difficult topic in such a simple way .

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@yuchuntsao0609]

Thank you very very much for your detail introduction of RNAi machanism! you are the best teacher.

[@anuhyavalluri4881]

Hi Suman sir...I feel the extreme joy of learning while watching your videos....Can you please make a video on RNA i antagonist process .....how the plant viruses suppress the RNA i ....

[@shomusbiologyofficial]

Thank you. I will do that in a separate video

[@anuhyavalluri4881]

Thank you sir....your efforts are much appreciated...

[@harshoza1399]

Your videos are really a great help. Wanted to ask: Can we Use RNAi in immunising the cell against the RNA/ genetic information of HIV viruses?

[@vijayasrivastava1928]

Brilliantly explain the topic...👏👏👏

[@rajeshkakkar8024]

Crystal clear. Great job.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@dstan16224]

Sir...If Dicer has endonuclease activity then why is it chopping off the siRNA from the sides as 9:30? Please explain it Sir. Thank you.

[@tribenisharma1879]

Best video I have seen on this topic... thanks a lot

[@shomusbiologyofficial]

You're welcome

[@Light_in_Dark00]

No doubt great teacher you are😊..but plz make it clear that..only siRNA need NPC complex for its transportation (from nucleus to cytoplasm) or miRNA and shRNA also need????

[@snehajoby3150]

Once I had dumped this rna interference part coz it was so hard..but now I understood everything and its easy..thanku so much sir..u are really awesome.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@sairam41053]

Thanku so much sir for your nice explanation 🙏

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@dharampalkamboj6324]

Thanku so much for such a valuable lacture.😀🤗

[@shomusbiologyofficial]

You're welcome

[@HaidaSOD]

Thank you so much, this is such a wonderful and clear lecture! You really explained everything perfectly well. Thanks for your effort!

[@muslihakamarudheen9800]

Sir, which books are u referred ? Plz tell me sir....it will help for my research work

[@AA-ne1fv]

Suman, thank you so much for your videos. They really help to understand the concepts thoroughly!

[@irfanbashir4958]

Ariba jo

[@markrao9216]

Great, practice a bit on process flow fluency avoiding corrections and hesitations you have mastery over the subject

[@rumiirum1143]

Dada u r just too awesome,pls keep helping us n god bless u

[@lovelybarbhuiya9554]

Thank you sir.Your videos help me a lot😊

[@shomusbiologyofficial]

You're welcome

[@n-sh2014]

Many thanks for the video. Very informative as usual. However, if I'm not wrong, there is a mistake. In the RNAi introductory video, you say Dicer is RNase H, but you say Argonaut is RNase H in this video.

[@preeti4786]

sir, RNase III is endonuclease so how can it cleave from ends (sides)??

[@mohammadkhashan7425]

Thank you very much, you are amazing. I have been following your tutorials for a long time. But in this video I think there is a mistake, you said RISC is constructed of slicer and Argonaut, this is a mistake because the slicer is Argonaut, but other parts are components like GW182.

[@reenukashyap1068]

Hey you are amazing in teaching and make concept so clear it help me a lot in my net preparation. Do you have ecology videos also If so please reply??

[@vandnakapil889]

U r best teacher.... thnkuuu so much fr making this concept so easy😊😊

[@shomusbiologyofficial]

Thank you for appreciating my efforts. Glad to hear that you are getting benefit from the videos

[@vandnakapil889]

Shomu's Biology .. this is ur hard work... u gave us best video lectures on every topic...

[@aiswaryaganga7646]

Sir whether the double stranded rna is produced from the target sequence and it will cleave the same target mrna

[@rajlaxmiprasad7087]

Respect for you.

[@niazmughal3394]

no wrds for ur works ......really impressive ☺☺

[@mikesinh]

gr8 how to do it practically on any cell..please show practical video or give us link

[@anjalisingh6036]

sir, its Very knowledge full video, it makes my whole concept clear about siRNA but i wanna know about the various types of siRNA like survivin and many more. So can u pls help me sir

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@BioVossy]

dude you rock this was such an immense help!!!!

[@psrivastava6993]

really amazing very clear concept.

[@shomusbiologyofficial]

Thank you

[@myworld4972]

Thank u so much sir , this topic is to easy to understand and thank u again

[@shomusbiologyofficial]

Glad to hear that you are getting benefit from the videos

[@nada.h.majeed]

Bigger thanks for you Gad bless you 🙏🙏🙏🙏

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@beech9443]

should it actually be that coding and non-coding strand of DNA is being transcribed, so RNA can base pair not transcription of RNA?

[@ozgunteksoy4398]

thank you for your trainer videos. I want to shoot video about siRNA designing on a specific example

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@Mertule93]

Can anyone explain what is the difference between Dicer and Drosha (also Slicer/Pasha)?

[@darshanjain7113]

Which strand remained attached to the RISC complex if it would of been the antisense one then and only then we get an inhibition effect of target mRNA.. what if that strand is a sense strand.. then the whole process will be futile?.. plz answer the query.. it was a helpful video though..

[@ganeshgr616]

wow.....Great explanation.....tq somuch...... .

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@sneha-zb1yg]

very helpful and very nicely explained.... thank you

[@halimakhatoon6722]

Sir does dsrna produce when the plant get infected with virus or its present already bin plant???

[@jagmeetkaur2190]

Amazing! Thank you ☺️☺️☺️!!

[@shomusbiologyofficial]

You're welcome

[@dondapatisrujan8351]

Very nice presentation

[@shomusbiologyofficial]

Thank you

[@varsharamachandran4043]

RNase III is a endonuclease enzyme and how it able to cut in the sides or in the end of the strands ?????

[@shaz781]

You are outstandingggggg. Love from Pakistan👍subscribed.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

[@nutribox4535]

my overseas professor of all time

[@shomusbiologyofficial]

Thank you

[@pramodkudash2817]

Sir, can u tell me if this can work in acellular organisms

[@dr.balasubramanianv3437]

What will happen to the passenger strand? whether its get degraded or not?

[@vishnudasan3711]

bro you say that in miRNA after forming RISC there are two domains one is paz that attach with single stranded mRNA & another is pivi domain have RNase activity..OK in all type of so,sh,mi RNA strands are unwinds by helicase?? are some factors involved??

[@akashmishra1285]

What are the intranuclear processing of a ds rna before leaving the nucleus??

[@Ale93Difo]

You made my day

[@shomusbiologyofficial]

Thank you

[@affbhaskar]

No words to express 🤗

[@shomusbiologyofficial]

Thank you

[@bdhome2010]

you are just fabulous.

[@shomusbiologyofficial]

Thank you for watching the lecture

[@gokulkrishnan1371]

very nice teaching sir.....

[@peacemorepeace2000]

have u cleared net jrf but very nicely and intelligently done

[@jilmilmahanta3669]

u r d best.. sir thank you so mch fr ds amazng descriptn abt Rnai

[@BalaBiologyWorld]

auspicious video dada....

[@karanamraghavendra6848]

thank you very much. very good explanation.

[@abhiluitsharma1355]

doesn't the helicase come into play in mi RNA processing?? pls specify as I'm having doubt

[@ritikasingh8809]

After cleaving the mRNA , whats the fate of guide strand further??

[@saidharani6731]

Excellent sir tq sooo much 🙏🙏🙏🙏🙏🙏🙏🙏🙏

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@firdausgulashan9228]

Super very understanding thanks

[@akanshasingh1746]

sir most of the plant viruses are single standed so what is the use of rna silencing in case of plant-virus system

[@ravleenpannu1573]

Amazing explanation 👏🏻

[@shomusbiologyofficial]

Thank you

[@rameshv8429]

Amazing explanation about interference mechanism thanks sir

[@selvamselvam1225]

This method use in Corona disease ??????

[@bharatnagesh9859]

RISC , (RNA induce silencing complex) not RNA interfering silencing complex

[@shomusbiologyofficial]

Yes. Thanks for the correction.

[@Megha-ij8eo]

Yes...u r correct...its RNA induced silencing complex...👍

[@vijayanagpurkar3875]

If so rna block the expression of m-RNA then how normal translational takes place

[@shakilakhtar5505]

Sir Can you please recommend some molecular biology books.

[@shomusbiologyofficial]

Gene xii

[@umashankarpatel4366]

Very useful sir, thank you sir

[@shrutireddy6151]

what about the guide rna strand?? after cleaving the target rna strand what happens to the guide rna

[@maryshelly6163]

very clear

[@shomusbiologyofficial]

Thank you

[@nimomalik3866]

thank you so much sir really helpful

[@ArivomThelivom]

How double stranded Rna is introduced

[@esmeraldasish]

Cloud you please do videos on MLS 4550 Clinical Immunohematology?

[@SUMITKUMAR-ws7rs]

very helpful 😊

[@srijamukherjee1394]

Very nice

[@vishnudasan3711]

dicer(RNase III) cleave ds RNA in pre mi RNA in cytoplasm and drasha cleave mi RNA in pri mi RNA form..

[@PankajSinghNishad9691]

Thanks sir ........

[@arpanchakrabarty2464]

fabulous sir

[@shomusbiologyofficial]

+Arpan Chakrabarty you're welcome

[@sijiphilip9618]

Thank you dude

[@FakhrurrazieJaffar]

Could it potentially cure HIV in the future? RNAi seems to be able to tamper with RNA replication of viral RNA in cells.

[@shomusbiologyofficial]

It has huge potential along with crispr cas 9 mechanism

[@sagarmovaliya9006]

One requesting for make pi or Piwi rna

[@wycliffenyandika9017]

nice!

[@shomusbiologyofficial]

Thank you

[@amruthamavily]

9.23 endonuclease...how??dicer is cutting from the end...then how?

[@nayanasarath3368]

sir I need online study of my Pg medical microbiology course

[@shomusbiologyofficial]

Please contact us at 9804654228 over WhatsApp for details

[@savitakamble4854]

very useful thnks

[@shomusbiologyofficial]

+sau Kamble you're welcome

[@savitakamble4854]

☺

[@aizaibelalima1064]

Thank you so much!!!!! :)